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The map label for this gene is nifS [H]

Identifier: 109947990

GI number: 109947990

Start: 1300769

End: 1301932

Strand: Reverse

Name: nifS [H]

Synonym: Hac_1498

Alternate gene names: 109947990

Gene position: 1301932-1300769 (Counterclockwise)

Preceding gene: 109947991

Following gene: 109947989

Centisome position: 83.78

GC content: 43.81

Gene sequence:

>1164_bases
TTGTTACAACGAATTTATTTAGACAATAACGCTACAACGAAAATTGACCCTAAAGTTAGAGCAATCATGGATCCCTTTTT
AAGGGATCACTATGGGAATCCTAGCTCTTTGCACCAATTTGGCACAGAAACCCACCCAGCCATTGCTGAAGCTTTAGACA
AGCTTTATAGGGGCATTAACGCTAGAGATATAGACGATGTGATCATCACTTCTTGTGCAACGGAGAGCAATAATTGGGTT
TTAAAGGGTGTGTATTTTGATGAATGCTTGAAAAAAGGCAAGAATCATATTGTTACCACGGTTGCAGAACATCCGGCGGT
GCGATCCACTTGTAATTTTTTAGAAAGCTTGGGAGTAAAGGTAACTTACTTACCTATCAATGAGCATGGGAGTATCACCG
CAGAGCAAGTCAAAGAAGCGATCACAGACAAAACCGCTTTAGTGAGCGTGATGTGGGCGAATAATGAAACCGGTCTCATT
TTCCCTATTGAAGAAATTGGGGTTATTTGTAAAGAAAAGGGCGTGTTATTCCATACCGATGCGGTGCAAGCGATTGGTAA
AATCCCTGTAGATGTGATAAAAGCGAATGCGGATTTCCTTTCTTTTAGCGCACATAAGTTTCATGGGCCTAAAGGCGTTG
GGGGGTTATATATTAGAAGTGGGGTGGAATTGACCCCTCTTTTTCATGGTGGGGAGCATATGAATGGCAGGCGCAGTGGG
ACTTTGAATGTGCCTTATATTGTGGGCATGGGCGAAGCGATGAGATTAGCCGTAGAGCATTTAGACTATGAAAAAGAAGT
GGTAGGGAAATTGCGCGACAAATTAGAAGGAGCGCTTTTGAAAATCCCTGATGTGATGGTGGTAGGCGATCGAGTCCATC
GTGTGCCTAACACGACTTTAATCAGCGTGAGAGGGATTGAAGGGGAGGCTATGTTATGGGATTTAAACCGCGCTAATATC
GCCGCTTCCACAGGGAGCGCGTGCGCGAGTGAGGATTTGGAGGCTAATCCTGTAATGGTGGCGATTGGGGCGAGTAAGGA
GTTAGCTCATACCGCTATCAGGCTTTCATTGAGCCGTTTTAACACGGAAGCTGAAATTGACAAAACGATTGAAGTTTTCT
CTCAAGCGGCTACAAGATTGAGAAATATTTCAAGCTCTTATTAA

Upstream 100 bases:

>100_bases
AATTCATCTTATTTTTAAGTTAAAATTAAGAAAATATCTTGTATATCACTAACCGACTTGGATAAGAAATCTATGCAAAA
TTTTTAAGGAGAACGCTCAT

Downstream 100 bases:

>100_bases
AATACCAATAAAGGAATCAAAATGGCAAAACATGATTTAGTGGGATCAGTCCTTTGGGATGCGTATTCTAAAGAAGTTCA
AAGACGCATGGATAACCCTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLQRIYLDNNATTKIDPKVRAIMDPFLRDHYGNPSSLHQFGTETHPAIAEALDKLYRGINARDIDDVIITSCATESNNWV
LKGVYFDECLKKGKNHIVTTVAEHPAVRSTCNFLESLGVKVTYLPINEHGSITAEQVKEAITDKTALVSVMWANNETGLI
FPIEEIGVICKEKGVLFHTDAVQAIGKIPVDVIKANADFLSFSAHKFHGPKGVGGLYIRSGVELTPLFHGGEHMNGRRSG
TLNVPYIVGMGEAMRLAVEHLDYEKEVVGKLRDKLEGALLKIPDVMVVGDRVHRVPNTTLISVRGIEGEAMLWDLNRANI
AASTGSACASEDLEANPVMVAIGASKELAHTAIRLSLSRFNTEAEIDKTIEVFSQAATRLRNISSSY

Sequences:

>Translated_387_residues
MLQRIYLDNNATTKIDPKVRAIMDPFLRDHYGNPSSLHQFGTETHPAIAEALDKLYRGINARDIDDVIITSCATESNNWV
LKGVYFDECLKKGKNHIVTTVAEHPAVRSTCNFLESLGVKVTYLPINEHGSITAEQVKEAITDKTALVSVMWANNETGLI
FPIEEIGVICKEKGVLFHTDAVQAIGKIPVDVIKANADFLSFSAHKFHGPKGVGGLYIRSGVELTPLFHGGEHMNGRRSG
TLNVPYIVGMGEAMRLAVEHLDYEKEVVGKLRDKLEGALLKIPDVMVVGDRVHRVPNTTLISVRGIEGEAMLWDLNRANI
AASTGSACASEDLEANPVMVAIGASKELAHTAIRLSLSRFNTEAEIDKTIEVFSQAATRLRNISSSY
>Mature_387_residues
MLQRIYLDNNATTKIDPKVRAIMDPFLRDHYGNPSSLHQFGTETHPAIAEALDKLYRGINARDIDDVIITSCATESNNWV
LKGVYFDECLKKGKNHIVTTVAEHPAVRSTCNFLESLGVKVTYLPINEHGSITAEQVKEAITDKTALVSVMWANNETGLI
FPIEEIGVICKEKGVLFHTDAVQAIGKIPVDVIKANADFLSFSAHKFHGPKGVGGLYIRSGVELTPLFHGGEHMNGRRSG
TLNVPYIVGMGEAMRLAVEHLDYEKEVVGKLRDKLEGALLKIPDVMVVGDRVHRVPNTTLISVRGIEGEAMLWDLNRANI
AASTGSACASEDLEANPVMVAIGASKELAHTAIRLSLSRFNTEAEIDKTIEVFSQAATRLRNISSSY

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=388, Percent_Identity=42.7835051546392, Blast_Score=299, Evalue=2e-81,
Organism=Homo sapiens, GI156713448, Length=423, Percent_Identity=33.096926713948, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=42.8571428571429, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1787970, Length=392, Percent_Identity=23.7244897959184, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1789175, Length=345, Percent_Identity=24.9275362318841, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=387, Percent_Identity=42.3772609819121, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=34.4512195121951, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=383, Percent_Identity=43.6031331592689, Blast_Score=289, Evalue=7e-79,
Organism=Drosophila melanogaster, GI20129463, Length=387, Percent_Identity=42.8940568475452, Blast_Score=290, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017773
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42387; Mature: 42387

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQRIYLDNNATTKIDPKVRAIMDPFLRDHYGNPSSLHQFGTETHPAIAEALDKLYRGIN
CCEEEEECCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCC
ARDIDDVIITSCATESNNWVLKGVYFDECLKKGKNHIVTTVAEHPAVRSTCNFLESLGVK
CCCCHHHHHEEECCCCCCEEEEEEEHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCE
VTYLPINEHGSITAEQVKEAITDKTALVSVMWANNETGLIFPIEEIGVICKEKGVLFHTD
EEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEEEHHHHCHHCCCCCEEEECH
AVQAIGKIPVDVIKANADFLSFSAHKFHGPKGVGGLYIRSGVELTPLFHGGEHMNGRRSG
HHHHHHCCCCEEEECCCCEEEECHHHCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCC
TLNVPYIVGMGEAMRLAVEHLDYEKEVVGKLRDKLEGALLKIPDVMVVGDRVHRVPNTTL
CCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCEEEECCHHHCCCCCEE
ISVRGIEGEAMLWDLNRANIAASTGSACASEDLEANPVMVAIGASKELAHTAIRLSLSRF
EEEECCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHC
NTEAEIDKTIEVFSQAATRLRNISSSY
CCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLQRIYLDNNATTKIDPKVRAIMDPFLRDHYGNPSSLHQFGTETHPAIAEALDKLYRGIN
CCEEEEECCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCC
ARDIDDVIITSCATESNNWVLKGVYFDECLKKGKNHIVTTVAEHPAVRSTCNFLESLGVK
CCCCHHHHHEEECCCCCCEEEEEEEHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCE
VTYLPINEHGSITAEQVKEAITDKTALVSVMWANNETGLIFPIEEIGVICKEKGVLFHTD
EEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEEEHHHHCHHCCCCCEEEECH
AVQAIGKIPVDVIKANADFLSFSAHKFHGPKGVGGLYIRSGVELTPLFHGGEHMNGRRSG
HHHHHHCCCCEEEECCCCEEEECHHHCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCC
TLNVPYIVGMGEAMRLAVEHLDYEKEVVGKLRDKLEGALLKIPDVMVVGDRVHRVPNTTL
CCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCEEEECCHHHCCCCCEE
ISVRGIEGEAMLWDLNRANIAASTGSACASEDLEANPVMVAIGASKELAHTAIRLSLSRF
EEEECCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHC
NTEAEIDKTIEVFSQAATRLRNISSSY
CCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA