Click here to switch to the map view.

The map label for this gene is purA

Identifier: 109947862

GI number: 109947862

Start: 1172295

End: 1173530

Strand: Reverse

Name: purA

Synonym: Hac_1353

Alternate gene names: 109947862

Gene position: 1173530-1172295 (Counterclockwise)

Preceding gene: 109947863

Following gene: 109947861

Centisome position: 75.52

GC content: 42.39

Gene sequence:

>1236_bases
ATGGCAGATGTCGTTGTAGGGATCCAGTGGGGAGATGAGGGGAAGGGAAAAATTGTGGATAGAATCGCTAAAGATTATGA
CTTTGTGGTGCGCTATCAAGGTGGGCATAACGCAGGGCATACCATTGTGCATAAGGGGGTTAAGCATTCTTTGCATTTAA
TGCCCTCAGGGGTGCTCTACTCTCAATGCAAGAATATCATTTCTAGCGCGGTGGTCGTGAGCGTTAAGGATTTGTGCGAA
GAAATCAGCATGTTTGAAAATTTAGAAAATCGTTTGTTTGTTAGCGATAGAGCCCATGTGATCTTGCCCTACCATGTGCA
AAAAGACGCTTTTAAAGAAAAATCTCAAAACATCGGCACGACTAAAAAAGGCATAGGCCCTTGCTATGAGGATAAAATGG
CAAGGAGCGGAATAAGAATGGGGGATTTATTAGACGATAGAATTTTAGAAGAAAAGTTAAAGGTGCATTTCAAAGCCATT
GAGCCTTTTAAAGAAGCGTATGATTTGAATGAAACTTACGAAAAAGATTTGAGGGAGTATTTTGAACAATATGCCCCAAA
AATCCGCCCCTTTATCAAAGACACGACCAGCATGCTGATAGAAGCCAATCAAAAGGGCGAAAAAATCTTACTAGAAGGGG
CGCAAGGCACGCTTTTAGACATTGATTTGGGGACTTACCCTTTTGTCACAAGCTCTAACACCACGAGCGCTAGTGCATGC
GTGAGCACCGGCTTAAACCCTAAAGCGATCAATGAGATTATAGGTATCACAAAAGCTTATTCCACTCGTGTGGGGAATGG
GCCTTTCCCTAGTGAAGACACTACGCCTATGGGGGATCATTTAAGAATTGGGGGTGCTGAGTTTGGTACGACGACAAAAC
GCCCAAGGCGTTGCGGTTGGTTGGATTTGGTGGCTTTAAAATACGCTTGCGCCTTGAATGGTTGCACGCAATTAGCTTTA
ATGAAATTAGATGTTTTAGACGGAATCAATGAAATTAAGGTGTGCGTGGCTTATGAAAGAAGGGGTGAAAAATTAGAGAC
TTTCCCTAGCGATTTGAAAGATTGCGTGCCGATTTATCAAACTTTTAAGGGCTGGGAAAAAAGCGTGGGGGTGAGAAAAT
TAGACGATTTAGAGCAAAACACTAGAGAGTATATCCGTTTTATTGAAGAAGAAGTGGGGGTAAAAATCCGCCTTATTTCT
ACAAGCCCTGAAAGAGAAGACACGATTTTTCTATGA

Upstream 100 bases:

>100_bases
TATCAATTACACGATAGGTTTTTAAGCTCTTTTTGGGGCTTTGCTTTTATTCTAATAAGCTTTTAAACAATCCAATCTAC
TTTATTTTAAGGGTAATTTT

Downstream 100 bases:

>100_bases
AAAAATTCGCTTCTGTGTTGGTGCAATTAAAGACTCTTGCGTTAGAAAAAATAGAGCAAAAGCTTCAAAACAAACGCCTG
GAGTTGCAACAAAATGAACA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MADVVVGIQWGDEGKGKIVDRIAKDYDFVVRYQGGHNAGHTIVHKGVKHSLHLMPSGVLYSQCKNIISSAVVVSVKDLCE
EISMFENLENRLFVSDRAHVILPYHVQKDAFKEKSQNIGTTKKGIGPCYEDKMARSGIRMGDLLDDRILEEKLKVHFKAI
EPFKEAYDLNETYEKDLREYFEQYAPKIRPFIKDTTSMLIEANQKGEKILLEGAQGTLLDIDLGTYPFVTSSNTTSASAC
VSTGLNPKAINEIIGITKAYSTRVGNGPFPSEDTTPMGDHLRIGGAEFGTTTKRPRRCGWLDLVALKYACALNGCTQLAL
MKLDVLDGINEIKVCVAYERRGEKLETFPSDLKDCVPIYQTFKGWEKSVGVRKLDDLEQNTREYIRFIEEEVGVKIRLIS
TSPEREDTIFL

Sequences:

>Translated_411_residues
MADVVVGIQWGDEGKGKIVDRIAKDYDFVVRYQGGHNAGHTIVHKGVKHSLHLMPSGVLYSQCKNIISSAVVVSVKDLCE
EISMFENLENRLFVSDRAHVILPYHVQKDAFKEKSQNIGTTKKGIGPCYEDKMARSGIRMGDLLDDRILEEKLKVHFKAI
EPFKEAYDLNETYEKDLREYFEQYAPKIRPFIKDTTSMLIEANQKGEKILLEGAQGTLLDIDLGTYPFVTSSNTTSASAC
VSTGLNPKAINEIIGITKAYSTRVGNGPFPSEDTTPMGDHLRIGGAEFGTTTKRPRRCGWLDLVALKYACALNGCTQLAL
MKLDVLDGINEIKVCVAYERRGEKLETFPSDLKDCVPIYQTFKGWEKSVGVRKLDDLEQNTREYIRFIEEEVGVKIRLIS
TSPEREDTIFL
>Mature_410_residues
ADVVVGIQWGDEGKGKIVDRIAKDYDFVVRYQGGHNAGHTIVHKGVKHSLHLMPSGVLYSQCKNIISSAVVVSVKDLCEE
ISMFENLENRLFVSDRAHVILPYHVQKDAFKEKSQNIGTTKKGIGPCYEDKMARSGIRMGDLLDDRILEEKLKVHFKAIE
PFKEAYDLNETYEKDLREYFEQYAPKIRPFIKDTTSMLIEANQKGEKILLEGAQGTLLDIDLGTYPFVTSSNTTSASACV
STGLNPKAINEIIGITKAYSTRVGNGPFPSEDTTPMGDHLRIGGAEFGTTTKRPRRCGWLDLVALKYACALNGCTQLALM
KLDVLDGINEIKVCVAYERRGEKLETFPSDLKDCVPIYQTFKGWEKSVGVRKLDDLEQNTREYIRFIEEEVGVKIRLIST
SPEREDTIFL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=44.6511627906977, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=42.3887587822014, Blast_Score=339, Evalue=4e-93,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=40.7035175879397, Blast_Score=298, Evalue=4e-81,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=46.9483568075117, Blast_Score=355, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI25146555, Length=423, Percent_Identity=41.1347517730496, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI25146553, Length=423, Percent_Identity=41.1347517730496, Blast_Score=323, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=42.6573426573427, Blast_Score=355, Evalue=8e-99,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=44.9411764705882, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_HELAH (Q17W85)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_665090.1
- ProteinModelPortal:   Q17W85
- SMR:   Q17W85
- STRING:   Q17W85
- GeneID:   4177742
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1353
- NMPDR:   fig|382638.8.peg.1312
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q17W85
- ProtClustDB:   PRK01117
- BioCyc:   HACI382638:HAC_1353-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46147; Mature: 46016

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 12-12 ACT_SITE 40-40 BINDING 121-121 BINDING 135-135 BINDING 215-215 BINDING 230-230 BINDING 294-294 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVVVGIQWGDEGKGKIVDRIAKDYDFVVRYQGGHNAGHTIVHKGVKHSLHLMPSGVLY
CCCEEEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHH
SQCKNIISSAVVVSVKDLCEEISMFENLENRLFVSDRAHVILPYHVQKDAFKEKSQNIGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEEECCHHHHHHHHCCCCC
TKKGIGPCYEDKMARSGIRMGDLLDDRILEEKLKVHFKAIEPFKEAYDLNETYEKDLREY
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FEQYAPKIRPFIKDTTSMLIEANQKGEKILLEGAQGTLLDIDLGTYPFVTSSNTTSASAC
HHHHCHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCEEEEECCCCCEEECCCCCCHHHH
VSTGLNPKAINEIIGITKAYSTRVGNGPFPSEDTTPMGDHLRIGGAEFGTTTKRPRRCGW
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCH
LDLVALKYACALNGCTQLALMKLDVLDGINEIKVCVAYERRGEKLETFPSDLKDCVPIYQ
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEHHCCCHHHHCHHHHHHHHHHHH
TFKGWEKSVGVRKLDDLEQNTREYIRFIEEEVGVKIRLISTSPEREDTIFL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ADVVVGIQWGDEGKGKIVDRIAKDYDFVVRYQGGHNAGHTIVHKGVKHSLHLMPSGVLY
CCEEEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHH
SQCKNIISSAVVVSVKDLCEEISMFENLENRLFVSDRAHVILPYHVQKDAFKEKSQNIGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEEECCHHHHHHHHCCCCC
TKKGIGPCYEDKMARSGIRMGDLLDDRILEEKLKVHFKAIEPFKEAYDLNETYEKDLREY
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FEQYAPKIRPFIKDTTSMLIEANQKGEKILLEGAQGTLLDIDLGTYPFVTSSNTTSASAC
HHHHCHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCEEEEECCCCCEEECCCCCCHHHH
VSTGLNPKAINEIIGITKAYSTRVGNGPFPSEDTTPMGDHLRIGGAEFGTTTKRPRRCGW
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCH
LDLVALKYACALNGCTQLALMKLDVLDGINEIKVCVAYERRGEKLETFPSDLKDCVPIYQ
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEHHCCCHHHHCHHHHHHHHHHHH
TFKGWEKSVGVRKLDDLEQNTREYIRFIEEEVGVKIRLISTSPEREDTIFL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA