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The map label for this gene is pyrB

Identifier: 109947358

GI number: 109947358

Start: 695400

End: 696326

Strand: Direct

Name: pyrB

Synonym: Hac_0799

Alternate gene names: 109947358

Gene position: 695400-696326 (Clockwise)

Preceding gene: 109947348

Following gene: 109947359

Centisome position: 44.75

GC content: 38.83

Gene sequence:

>927_bases
ATGCCAAAACAATGCCGACACTTGCTCCAAACTAGCGATTTGAGCATGGATGAAATCAAGCTTTTATTAAAAAAAGCGAG
CGTTTATGCGAACGATTTTAACGCGGTTTCTTTAGAGGTAAAAGAAAAAATACATAATAAAATCATCGTGGCTTTATTTT
TTGAAAATTCCACTAGAACGGTGTCTAGCTTTGAAATCGCAAGCTTGAGGTTGGGGGCAAAAATGGTGAAATTAAACATG
CAAACAAGCTCCACTTCAAAGGGTGAAACTTTAATAGACACTTTTAAAAATATCCATGCTATGCAACCTGATGCTATTAT
TATGCGCCATGCCTTTTCAAGCGCACCTTTTAAATTGGTAGAATTTTCACAATGCCCCTTAATCAATGCAGGGAGTGGTA
CAAGCGCTCATCCTACCCAAGCGTTATTGGATTTACTCACTCTTTATCAACATTTTGGTAGTTTAGAAAATTTGAAAGGG
AAAAAAATCGCTTTCATAGGCGATGTGAAAAATTCAAGAGTGGCTAATAGCAATATCAAATTGCTCCAAAGACTAGGGCT
TGAGATAATGCTATGCGCTCCAAGCTCCATGCTCCCTAGCACTCCTTTAATAAAAACGACGCACCACATTGGAGAAGCGA
TAGAGTTTGCAGATATCTTAATGAGCTTGAGAACCCAAACCGAACGCCACAATACGCCTATTTTTGCGAGCTTGAAAGAT
TATGCTAACGCCTATTGTATCACTCAAAAACGCTTATATGATCACGCTAAAAATAAAGAAGTGGTTATCCTGCATCCAGG
TCCGGTGCATAGGGATATTGATATAGAAAGCACTATGTTAGAAGATAGGCGATCTAAAGTCTTAGAGCAAGTCAAAAATG
GCGTGGCAATGCGCATGGCGGTGTTGGAGTTTTTATTGGTAGATTAA

Upstream 100 bases:

>100_bases
ATACTAGAAGTATTGAGAGATTTTTCAAGCCCATTCCTTGCTAAAACCCATGCCATTTTTAGCTAAAATAAAAGCCATAT
TATAACCAAAAGGGGGTTTT

Downstream 100 bases:

>100_bases
GTAACAACAAACCTAAAAACTTCAATTTTGACTCTATTTTTACATAAGTGTGAATGCTAAATTTTTTATAGTTTTAGATT
TAATTTTATAGTAAAACTCA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MPKQCRHLLQTSDLSMDEIKLLLKKASVYANDFNAVSLEVKEKIHNKIIVALFFENSTRTVSSFEIASLRLGAKMVKLNM
QTSSTSKGETLIDTFKNIHAMQPDAIIMRHAFSSAPFKLVEFSQCPLINAGSGTSAHPTQALLDLLTLYQHFGSLENLKG
KKIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSTPLIKTTHHIGEAIEFADILMSLRTQTERHNTPIFASLKD
YANAYCITQKRLYDHAKNKEVVILHPGPVHRDIDIESTMLEDRRSKVLEQVKNGVAMRMAVLEFLLVD

Sequences:

>Translated_308_residues
MPKQCRHLLQTSDLSMDEIKLLLKKASVYANDFNAVSLEVKEKIHNKIIVALFFENSTRTVSSFEIASLRLGAKMVKLNM
QTSSTSKGETLIDTFKNIHAMQPDAIIMRHAFSSAPFKLVEFSQCPLINAGSGTSAHPTQALLDLLTLYQHFGSLENLKG
KKIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSTPLIKTTHHIGEAIEFADILMSLRTQTERHNTPIFASLKD
YANAYCITQKRLYDHAKNKEVVILHPGPVHRDIDIESTMLEDRRSKVLEQVKNGVAMRMAVLEFLLVD
>Mature_307_residues
PKQCRHLLQTSDLSMDEIKLLLKKASVYANDFNAVSLEVKEKIHNKIIVALFFENSTRTVSSFEIASLRLGAKMVKLNMQ
TSSTSKGETLIDTFKNIHAMQPDAIIMRHAFSSAPFKLVEFSQCPLINAGSGTSAHPTQALLDLLTLYQHFGSLENLKGK
KIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSTPLIKTTHHIGEAIEFADILMSLRTQTERHNTPIFASLKDY
ANAYCITQKRLYDHAKNKEVVILHPGPVHRDIDIESTMLEDRRSKVLEQVKNGVAMRMAVLEFLLVD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=276, Percent_Identity=32.2463768115942, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI2367364, Length=320, Percent_Identity=33.4375, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1790703, Length=291, Percent_Identity=27.1477663230241, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786469, Length=213, Percent_Identity=28.169014084507, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994908, Length=214, Percent_Identity=31.3084112149533, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=289, Percent_Identity=33.5640138408304, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=321, Percent_Identity=34.5794392523364, Blast_Score=145, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=24.6951219512195, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24642586, Length=283, Percent_Identity=33.2155477031802, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_HELAH (Q17XN9)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664586.1
- ProteinModelPortal:   Q17XN9
- SMR:   Q17XN9
- STRING:   Q17XN9
- GeneID:   4177749
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0799
- NMPDR:   fig|382638.8.peg.775
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   HACI382638:HAC_0799-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34488; Mature: 34357

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQCRHLLQTSDLSMDEIKLLLKKASVYANDFNAVSLEVKEKIHNKIIVALFFENSTRT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCEEEEEEEECCCCCE
VSSFEIASLRLGAKMVKLNMQTSSTSKGETLIDTFKNIHAMQPDAIIMRHAFSSAPFKLV
EHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEE
EFSQCPLINAGSGTSAHPTQALLDLLTLYQHFGSLENLKGKKIAFIGDVKNSRVANSNIK
ECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCHHH
LLQRLGLEIMLCAPSSMLPSTPLIKTTHHIGEAIEFADILMSLRTQTERHNTPIFASLKD
HHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
YANAYCITQKRLYDHAKNKEVVILHPGPVHRDIDIESTMLEDRRSKVLEQVKNGVAMRMA
HCCEEEEEHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
VLEFLLVD
HHHHHHCC
>Mature Secondary Structure 
PKQCRHLLQTSDLSMDEIKLLLKKASVYANDFNAVSLEVKEKIHNKIIVALFFENSTRT
CHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCEEEEEEEECCCCCE
VSSFEIASLRLGAKMVKLNMQTSSTSKGETLIDTFKNIHAMQPDAIIMRHAFSSAPFKLV
EHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEE
EFSQCPLINAGSGTSAHPTQALLDLLTLYQHFGSLENLKGKKIAFIGDVKNSRVANSNIK
ECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCHHH
LLQRLGLEIMLCAPSSMLPSTPLIKTTHHIGEAIEFADILMSLRTQTERHNTPIFASLKD
HHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
YANAYCITQKRLYDHAKNKEVVILHPGPVHRDIDIESTMLEDRRSKVLEQVKNGVAMRMA
HCCEEEEEHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
VLEFLLVD
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA