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The map label for this gene is ddl

Identifier: 109947256

GI number: 109947256

Start: 596866

End: 597918

Strand: Reverse

Name: ddl

Synonym: Hac_0681

Alternate gene names: 109947256

Gene position: 597918-596866 (Counterclockwise)

Preceding gene: 109947257

Following gene: 109947255

Centisome position: 38.48

GC content: 34.09

Gene sequence:

>1053_bases
ATGGTGGAATTTTGCGTTTTATTTGGTGGGGCGAGTTTTGAACATGAAATCAGTATTGTGAGTGCAATCGCACTTAAAGA
AGTGTTGAAAGATCAAATTAAATATTTTATTTTTTTAGATGAAAACCATCATTTTTACTTGATTGAAGCATCTAACATGC
ATTCAAAATACTTCGCTCAAATCAAAGAAAAAAAATTACCCCCCCTAATGCTGACACACAATGGTTTACTTAAAAACTCG
TTTTTAGGCGCTAAAACAATAGAATTACCTTTAATGATTAACCTTGTGCATGGAGGCGATGGCGAAGACGGAAAATTAGC
GAGCTTGTTAGAATTTTATCGTATCGCTTTTATAGGCTCTAGAGTTGAAGCGAGCGTGTTGAGCTATAACAAACATTTAA
CCAAGCTTTATGCTAAAAATTTAGGGGTAAAGACTTTAGATTGCATTCTTTTGAATGAAAAAAACCGTGCTAACGCCCTG
GATTTGATCAAGCCTAGTTTTAATTTTCCCTTTATTGTCAAACCCAATAACGCTGGGAGCTCTTTAGGGGTGAGTGTTGT
GAAAGAAGAAAAAGAATTAGCCTACGCTTTAGATGGCGCGTTTGAATATTCTAAAGAAGTCTTAATAGAACCTTTCATTC
AAAGAGTGAAAGAATACAATTTAGCCGGTTGTAAGATAAAAGAGGATTTTTGTTTTTCCTATGTTGAAGAGCCTAACAAA
CAAGAGTTTTTGGATTTCAAACAAAAATATTTGGATTTTTCACGCACCAAAGCCCCTAAAGCTAACATTCCTAACGCCCT
AGAAGAACAATTGAGAGAAAATTTTAAAAAACTCTATAACGATTTGTTTGATGGTGCGATCATTCGTTGCGATTTTTTTG
TCATAGATAACGAAGTGTATCTTAATGAGATCAACCCCATTCCTGGCAGTTTAGCCAATTATTTGTTTGATGATTTTAAA
ACAACGCTAGAAAATTTAGCCCAATCATTACCCAAAACCCCTAAAATCCAAGTTAAAAACTCTTATTTGTTACAGATCCA
AAAGAATAAGTAA

Upstream 100 bases:

>100_bases
TGGGGTTTTTTAACATTAAACTTTAATTTTAAGAAAATTCAAAAGCCTTTTTTTAGGTAAATATAGATAGACTCTATTTT
AATCGTTTTTAGGGAGTTTT

Downstream 100 bases:

>100_bases
TGGCTAAACGCAGCGTCGCTTATTTAGGGAGCGTTTTTGATATTTCCTACACTTTTATAGACAACCATAGCCCTTTAAAC
GCCTTGTTTTTGCATGGCTG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MVEFCVLFGGASFEHEISIVSAIALKEVLKDQIKYFIFLDENHHFYLIEASNMHSKYFAQIKEKKLPPLMLTHNGLLKNS
FLGAKTIELPLMINLVHGGDGEDGKLASLLEFYRIAFIGSRVEASVLSYNKHLTKLYAKNLGVKTLDCILLNEKNRANAL
DLIKPSFNFPFIVKPNNAGSSLGVSVVKEEKELAYALDGAFEYSKEVLIEPFIQRVKEYNLAGCKIKEDFCFSYVEEPNK
QEFLDFKQKYLDFSRTKAPKANIPNALEEQLRENFKKLYNDLFDGAIIRCDFFVIDNEVYLNEINPIPGSLANYLFDDFK
TTLENLAQSLPKTPKIQVKNSYLLQIQKNK

Sequences:

>Translated_350_residues
MVEFCVLFGGASFEHEISIVSAIALKEVLKDQIKYFIFLDENHHFYLIEASNMHSKYFAQIKEKKLPPLMLTHNGLLKNS
FLGAKTIELPLMINLVHGGDGEDGKLASLLEFYRIAFIGSRVEASVLSYNKHLTKLYAKNLGVKTLDCILLNEKNRANAL
DLIKPSFNFPFIVKPNNAGSSLGVSVVKEEKELAYALDGAFEYSKEVLIEPFIQRVKEYNLAGCKIKEDFCFSYVEEPNK
QEFLDFKQKYLDFSRTKAPKANIPNALEEQLRENFKKLYNDLFDGAIIRCDFFVIDNEVYLNEINPIPGSLANYLFDDFK
TTLENLAQSLPKTPKIQVKNSYLLQIQKNK
>Mature_350_residues
MVEFCVLFGGASFEHEISIVSAIALKEVLKDQIKYFIFLDENHHFYLIEASNMHSKYFAQIKEKKLPPLMLTHNGLLKNS
FLGAKTIELPLMINLVHGGDGEDGKLASLLEFYRIAFIGSRVEASVLSYNKHLTKLYAKNLGVKTLDCILLNEKNRANAL
DLIKPSFNFPFIVKPNNAGSSLGVSVVKEEKELAYALDGAFEYSKEVLIEPFIQRVKEYNLAGCKIKEDFCFSYVEEPNK
QEFLDFKQKYLDFSRTKAPKANIPNALEEQLRENFKKLYNDLFDGAIIRCDFFVIDNEVYLNEINPIPGSLANYLFDDFK
TTLENLAQSLPKTPKIQVKNSYLLQIQKNK

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=319, Percent_Identity=29.4670846394984, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786280, Length=225, Percent_Identity=31.1111111111111, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_HELAH (Q17XZ1)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664484.1
- ProteinModelPortal:   Q17XZ1
- SMR:   Q17XZ1
- STRING:   Q17XZ1
- GeneID:   4177122
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0681
- NMPDR:   fig|382638.8.peg.663
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   KYMDFSR
- ProtClustDB:   PRK01966
- BioCyc:   HACI382638:HAC_0681-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39998; Mature: 39998

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEFCVLFGGASFEHEISIVSAIALKEVLKDQIKYFIFLDENHHFYLIEASNMHSKYFAQ
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEEECCCCCHHHHHH
IKEKKLPPLMLTHNGLLKNSFLGAKTIELPLMINLVHGGDGEDGKLASLLEFYRIAFIGS
HHHCCCCCEEEECCCCHHCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
RVEASVLSYNKHLTKLYAKNLGVKTLDCILLNEKNRANALDLIKPSFNFPFIVKPNNAGS
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHEECCCCCCCEEEECCCCCC
SLGVSVVKEEKELAYALDGAFEYSKEVLIEPFIQRVKEYNLAGCKIKEDFCFSYVEEPNK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCCCCH
QEFLDFKQKYLDFSRTKAPKANIPNALEEQLRENFKKLYNDLFDGAIIRCDFFVIDNEVY
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECEEE
LNEINPIPGSLANYLFDDFKTTLENLAQSLPKTPKIQVKNSYLLQIQKNK
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEECCC
>Mature Secondary Structure
MVEFCVLFGGASFEHEISIVSAIALKEVLKDQIKYFIFLDENHHFYLIEASNMHSKYFAQ
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEEECCCCCHHHHHH
IKEKKLPPLMLTHNGLLKNSFLGAKTIELPLMINLVHGGDGEDGKLASLLEFYRIAFIGS
HHHCCCCCEEEECCCCHHCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
RVEASVLSYNKHLTKLYAKNLGVKTLDCILLNEKNRANALDLIKPSFNFPFIVKPNNAGS
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHEECCCCCCCEEEECCCCCC
SLGVSVVKEEKELAYALDGAFEYSKEVLIEPFIQRVKEYNLAGCKIKEDFCFSYVEEPNK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCCCCH
QEFLDFKQKYLDFSRTKAPKANIPNALEEQLRENFKKLYNDLFDGAIIRCDFFVIDNEVY
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECEEE
LNEINPIPGSLANYLFDDFKTTLENLAQSLPKTPKIQVKNSYLLQIQKNK
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA