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The map label for this gene is murF [H]

Identifier: 109947254

GI number: 109947254

Start: 594673

End: 596154

Strand: Reverse

Name: murF [H]

Synonym: Hac_0679

Alternate gene names: 109947254

Gene position: 596154-594673 (Counterclockwise)

Preceding gene: 109947255

Following gene: 109947253

Centisome position: 38.36

GC content: 35.9

Gene sequence:

>1482_bases
ATGCAAAGTCCTAGTTGGCTGGATTTAGCGTTTCGTTGGCTCTTTGTAACAGGGCTTGGCTATTATGTGATAACTTTATT
GCAATGGTATCATTACAGCGTGTTTAGGATCTTAACTAAGCATCATAAAATGCGTTGGCATGGGATTTATTTTGTATTGC
CTTTAGGGGTGTTTATGGGATCGTATGTTTTCAAAATACCGTTTGTTTTTGATTTCTTTTGCGGTGTTATTCAAATACCC
ATGCTCATTGTTTGGGCTAAACGCAACGACAAGCCTTTAGTTTTCACGCCAAGGGTGAAGCGCTTTTTTATTTTTTTATT
GTTATTTTTAATCTTGCATGAAATCTTAAACATAGAATTAGTTTCTTTAAGCGGGATCTCACTCGCGCTTGGCTATTTGT
GTTTATTCATATTCGTTTTAAGCACTTCTTTAATCTTTGAAAAAGTCTTATCCAAGCAGTATTTGCAAATCGCTAAAGAT
AAAATCGCCTCTTTAAAAAGTTTAAAAGTCATCGCCATTACTGGGAGTTTTGGGAAAACTAGCACCAAAAATTTCTTGCA
TCAAATCTTACAAACGAAATTCAATGTGCATGCAAGCCCTAAAAGCGTCAATACCCTTTTAGGCATTGCGAATGATATTA
ACCAAAATTTAGAGGATAGTAGCGAAATTTATATCGCTGAAGCTGGGGCAAGGAATAAGGGCGATATTAAAGAAATCACC
CATCTCATTGAACCACACCTTGCTGTGATCACAGAAGTGGGCGAACAGCATTTAGAATATTTTAAAACTTTAGAAAATAT
TTGCGAGACTAAAGCGGAGTTATTGGATTCAAAACGATTGGAAAACGCCTTTTGTTACTCTATAGAGACCATTAAACCCT
ATGCCCCTAACAACGCCCCTTTAATAGATTATTCTAGCTTGGTTAAAAACATCCAATCCACTTTAAAAGGCACTTTTTTT
GAAATGCTTTTAGATGGCGTTTGGGAAAGTTTTGGAACGAAAGTTTTAGGCAAATTTAACGCTTATAATATCGCTTTAGC
TATTTTAATCGCCAAGCATTTAGGTTTAGAAACAGAAAGGATCAAACAGCTTGTTTTGAATCTCAACCCTATTGCCCATC
GTTTGCAACTTTTGGAAGTGAATCAAAAAGTCATTATAGACGATAGCTTTAATGGGAATTTAAAAGGCATGCTAGAGGGT
ATTCGTTTAGCGAGTTTGTATGAAGGGCGTAAAGTCATTGTAACGCCGGGATTAGTGGAAAGCAATACAGAAAGCAATGA
AACTTTAGCGAAAAAAATCAATGAAGTCTTTGATGTCGCTATCATCACAGGGGAGTTAAATTCTAAAGTGATTGCGTCCC
ATTTAAAGAAACCTCAAAAAATCTTACTCAAAGATAAGGGGCAATTAGAAAATATCTTACAAGCCACTACGATTCAAGGC
GATTTGATTTTATTTGCTAATGACGCCCCCAATTACATTTAA

Upstream 100 bases:

>100_bases
ACCTTATTAAAAAAGAGCAAATTATTTGTTTTAGAAGGGGATCATTTCTTTTTTTTAAACCAAGCAAAAGAGATTGAAAA
ACTAATGGAGAATTATGATC

Downstream 100 bases:

>100_bases
GAAGTGAATATGCAACATTTATATGCCCCTTGGCGCGAAAATTATTTGAAAGAGAAAATTAAGAATTGTGTTTTTTGCGA
GATCTCTCAAAACCCTGTAA

Product: D-alanyl-D-alanine-adding enzyme

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MQSPSWLDLAFRWLFVTGLGYYVITLLQWYHYSVFRILTKHHKMRWHGIYFVLPLGVFMGSYVFKIPFVFDFFCGVIQIP
MLIVWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIELVSLSGISLALGYLCLFIFVLSTSLIFEKVLSKQYLQIAKD
KIASLKSLKVIAITGSFGKTSTKNFLHQILQTKFNVHASPKSVNTLLGIANDINQNLEDSSEIYIAEAGARNKGDIKEIT
HLIEPHLAVITEVGEQHLEYFKTLENICETKAELLDSKRLENAFCYSIETIKPYAPNNAPLIDYSSLVKNIQSTLKGTFF
EMLLDGVWESFGTKVLGKFNAYNIALAILIAKHLGLETERIKQLVLNLNPIAHRLQLLEVNQKVIIDDSFNGNLKGMLEG
IRLASLYEGRKVIVTPGLVESNTESNETLAKKINEVFDVAIITGELNSKVIASHLKKPQKILLKDKGQLENILQATTIQG
DLILFANDAPNYI

Sequences:

>Translated_493_residues
MQSPSWLDLAFRWLFVTGLGYYVITLLQWYHYSVFRILTKHHKMRWHGIYFVLPLGVFMGSYVFKIPFVFDFFCGVIQIP
MLIVWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIELVSLSGISLALGYLCLFIFVLSTSLIFEKVLSKQYLQIAKD
KIASLKSLKVIAITGSFGKTSTKNFLHQILQTKFNVHASPKSVNTLLGIANDINQNLEDSSEIYIAEAGARNKGDIKEIT
HLIEPHLAVITEVGEQHLEYFKTLENICETKAELLDSKRLENAFCYSIETIKPYAPNNAPLIDYSSLVKNIQSTLKGTFF
EMLLDGVWESFGTKVLGKFNAYNIALAILIAKHLGLETERIKQLVLNLNPIAHRLQLLEVNQKVIIDDSFNGNLKGMLEG
IRLASLYEGRKVIVTPGLVESNTESNETLAKKINEVFDVAIITGELNSKVIASHLKKPQKILLKDKGQLENILQATTIQG
DLILFANDAPNYI
>Mature_493_residues
MQSPSWLDLAFRWLFVTGLGYYVITLLQWYHYSVFRILTKHHKMRWHGIYFVLPLGVFMGSYVFKIPFVFDFFCGVIQIP
MLIVWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIELVSLSGISLALGYLCLFIFVLSTSLIFEKVLSKQYLQIAKD
KIASLKSLKVIAITGSFGKTSTKNFLHQILQTKFNVHASPKSVNTLLGIANDINQNLEDSSEIYIAEAGARNKGDIKEIT
HLIEPHLAVITEVGEQHLEYFKTLENICETKAELLDSKRLENAFCYSIETIKPYAPNNAPLIDYSSLVKNIQSTLKGTFF
EMLLDGVWESFGTKVLGKFNAYNIALAILIAKHLGLETERIKQLVLNLNPIAHRLQLLEVNQKVIIDDSFNGNLKGMLEG
IRLASLYEGRKVIVTPGLVESNTESNETLAKKINEVFDVAIITGELNSKVIASHLKKPQKILLKDKGQLENILQATTIQG
DLILFANDAPNYI

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=292, Percent_Identity=25.3424657534247, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 55869; Mature: 55869

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSPSWLDLAFRWLFVTGLGYYVITLLQWYHYSVFRILTKHHKMRWHGIYFVLPLGVFMG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYVFKIPFVFDFFCGVIQIPMLIVWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIEL
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCEEE
VSLSGISLALGYLCLFIFVLSTSLIFEKVLSKQYLQIAKDKIASLKSLKVIAITGSFGKT
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCC
STKNFLHQILQTKFNVHASPKSVNTLLGIANDINQNLEDSSEIYIAEAGARNKGDIKEIT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHH
HLIEPHLAVITEVGEQHLEYFKTLENICETKAELLDSKRLENAFCYSIETIKPYAPNNAP
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCC
LIDYSSLVKNIQSTLKGTFFEMLLDGVWESFGTKVLGKFNAYNIALAILIAKHLGLETER
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHCCCHHH
IKQLVLNLNPIAHRLQLLEVNQKVIIDDSFNGNLKGMLEGIRLASLYEGRKVIVTPGLVE
HHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCC
SNTESNETLAKKINEVFDVAIITGELNSKVIASHLKKPQKILLKDKGQLENILQATTIQG
CCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHCCCCHHHHHCCCHHHHHHHHHHEECC
DLILFANDAPNYI
CEEEEECCCCCCC
>Mature Secondary Structure
MQSPSWLDLAFRWLFVTGLGYYVITLLQWYHYSVFRILTKHHKMRWHGIYFVLPLGVFMG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYVFKIPFVFDFFCGVIQIPMLIVWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIEL
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCEEE
VSLSGISLALGYLCLFIFVLSTSLIFEKVLSKQYLQIAKDKIASLKSLKVIAITGSFGKT
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCC
STKNFLHQILQTKFNVHASPKSVNTLLGIANDINQNLEDSSEIYIAEAGARNKGDIKEIT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHH
HLIEPHLAVITEVGEQHLEYFKTLENICETKAELLDSKRLENAFCYSIETIKPYAPNNAP
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCC
LIDYSSLVKNIQSTLKGTFFEMLLDGVWESFGTKVLGKFNAYNIALAILIAKHLGLETER
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHCCCHHH
IKQLVLNLNPIAHRLQLLEVNQKVIIDDSFNGNLKGMLEGIRLASLYEGRKVIVTPGLVE
HHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCC
SNTESNETLAKKINEVFDVAIITGELNSKVIASHLKKPQKILLKDKGQLENILQATTIQG
CCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHCCCCHHHHHCCCHHHHHHHHHHEECC
DLILFANDAPNYI
CEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]