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The map label for this gene is eda [H]

Identifier: 109947045

GI number: 109947045

Start: 387498

End: 388142

Strand: Direct

Name: eda [H]

Synonym: Hac_0443

Alternate gene names: 109947045

Gene position: 387498-388142 (Clockwise)

Preceding gene: 109947044

Following gene: 109947046

Centisome position: 24.94

GC content: 40.0

Gene sequence:

>645_bases
ATGCAAAATAAAATACAAGATAAAGTAATAGAGGTTTTACAAATTAGCCCCATTGTCCCTGTGGTGGTGATTGAAGATCT
GAAAGACGCTGTGCCTTTAGCGCAAAGCCTAATAGAGGGGGGTATTCCAATCATAGAAGTTACTCTGCGTTCAAACTGCG
CTTTGGAAGCCATAGAGCTTATCGCTAAAAATGTGCCAAAAATGCATGTGGGTGCTGGCACAATTTTAAATCTTAATCAA
TTAGAACAAGCTCAAAATAGGGGGGCTGAATTTTTGATTAGCCCAGGTCTTACGCCTAGCCTTTTAGAATACGCAAAGAA
AAAAGACATGCCCCTAATACCTGGGGTTTCTAGCAGTAGTGAAGTCATGCAAGCTTTAGAGTTGGGCTATAACGCCTTAA
AATTTTTCCCAGCAGAATATTGCGGAGGCGCTAAACTTTTAAACGCTTTTAATGGCCCTTTTAAGGGGGTGAAATTTTGC
CCTACTGGGGGCATTAGCGTGGATAACATGCATGCTTATTTGAAATTAGAAAATGTTTTGTGCGTGGGAGGGAGTTGGCT
TACCCATAGAAATTTAGTGCAAAATAAAGAGTGGGATAAAATCATAGAACTTTGCAAACAAGCGTTGGCTAACATTAAAA
CCTAA

Upstream 100 bases:

>100_bases
TTTGGGCTAGGTAGGGAATTATTTACGAGCTTAAGATTGAATGTCAATACGGCTGAAGAGGGTGCCATGAGTTTTGGCAT
AAAAATATAAAGGATATAAA

Downstream 100 bases:

>100_bases
TTGGCATTTAAAATAATGTTTAAAATAATGGGAGTGTGGTTTTGTATTTGCTTAATAACACTATAATAAGATTTTTAATA
AAAAGGAGAATGATGATGTT

Product: 2-dehydro-3-deoxyphosphogluconate aldolase / 4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MQNKIQDKVIEVLQISPIVPVVVIEDLKDAVPLAQSLIEGGIPIIEVTLRSNCALEAIELIAKNVPKMHVGAGTILNLNQ
LEQAQNRGAEFLISPGLTPSLLEYAKKKDMPLIPGVSSSSEVMQALELGYNALKFFPAEYCGGAKLLNAFNGPFKGVKFC
PTGGISVDNMHAYLKLENVLCVGGSWLTHRNLVQNKEWDKIIELCKQALANIKT

Sequences:

>Translated_214_residues
MQNKIQDKVIEVLQISPIVPVVVIEDLKDAVPLAQSLIEGGIPIIEVTLRSNCALEAIELIAKNVPKMHVGAGTILNLNQ
LEQAQNRGAEFLISPGLTPSLLEYAKKKDMPLIPGVSSSSEVMQALELGYNALKFFPAEYCGGAKLLNAFNGPFKGVKFC
PTGGISVDNMHAYLKLENVLCVGGSWLTHRNLVQNKEWDKIIELCKQALANIKT
>Mature_214_residues
MQNKIQDKVIEVLQISPIVPVVVIEDLKDAVPLAQSLIEGGIPIIEVTLRSNCALEAIELIAKNVPKMHVGAGTILNLNQ
LEQAQNRGAEFLISPGLTPSLLEYAKKKDMPLIPGVSSSSEVMQALELGYNALKFFPAEYCGGAKLLNAFNGPFKGVKFC
PTGGISVDNMHAYLKLENVLCVGGSWLTHRNLVQNKEWDKIIELCKQALANIKT

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=187, Percent_Identity=49.7326203208556, Blast_Score=196, Evalue=7e-52,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23292; Mature: 23292

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKIQDKVIEVLQISPIVPVVVIEDLKDAVPLAQSLIEGGIPIIEVTLRSNCALEAIEL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
IAKNVPKMHVGAGTILNLNQLEQAQNRGAEFLISPGLTPSLLEYAKKKDMPLIPGVSSSS
HHHCCCCEEECCCCEECHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCHH
EVMQALELGYNALKFFPAEYCGGAKLLNAFNGPFKGVKFCPTGGISVDNMHAYLKLENVL
HHHHHHHHCHHHHHHCCHHHCCHHHHHHHCCCCCCCCEECCCCCCCCCCHHEEEEECCEE
CVGGSWLTHRNLVQNKEWDKIIELCKQALANIKT
EECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQNKIQDKVIEVLQISPIVPVVVIEDLKDAVPLAQSLIEGGIPIIEVTLRSNCALEAIEL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
IAKNVPKMHVGAGTILNLNQLEQAQNRGAEFLISPGLTPSLLEYAKKKDMPLIPGVSSSS
HHHCCCCEEECCCCEECHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCHH
EVMQALELGYNALKFFPAEYCGGAKLLNAFNGPFKGVKFCPTGGISVDNMHAYLKLENVL
HHHHHHHHCHHHHHHCCHHHCCHHHHHHHCCCCCCCCEECCCCCCCCCCHHEEEEECCEE
CVGGSWLTHRNLVQNKEWDKIIELCKQALANIKT
EECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]