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The map label for this gene is priA [H]

Identifier: 109947025

GI number: 109947025

Start: 364848

End: 366707

Strand: Reverse

Name: priA [H]

Synonym: Hac_0421

Alternate gene names: 109947025

Gene position: 366707-364848 (Counterclockwise)

Preceding gene: 109947026

Following gene: 109947022

Centisome position: 23.6

GC content: 37.04

Gene sequence:

>1860_bases
ATGTTCTACTACTTAATCGCTCCCTTAAAAAATAAAACCCCCCCTTTAACCTATTTTTCTAAAGAACAACACCAAAAAGG
AGTGTTAGTCAATATTAATTTAAGGAATAAAACGCTTTTAGGCGTCATTCTTGAAGAAGTTTCAAAACCCTCTTTTGAAT
GCTTGGAGCTAGAAAAAACCCCTTATTTTTTACTCCCCATTCAAATAGAACTTGCTGAATTTATCGCTCAATATTACTCT
GCTAGTCTCTCTTTAGTCTTAAGCCTTTTTGCCCCCTTTAAAGAATGCGATGTTGCAAAATTAGAAAAAATTAAGCCCAA
TCTTAATGGGTTAAGCCAAACGCAAACCAATGCTTTAAACGCATTGCAAAAACATTCCGCAAGCTTACTCTTTGGTGATA
CAGGTAGTGGGAAAACCGAAATTTATATGCATTTAATCGCTCAAATGTTAGAGCAAAAAAAAAGCGCTTTATTATTGGTG
CCAGAAATCGCTCTCACCCCTCAAATACAACAGCGCCTTAAAAAAGTCTTTAAAGAAAATTTAGGCTTGTGGCATAGCAA
ACTCTCTTTAACTCAAAAAAAACAATTTTTAGAAAAGCTTTACTCACAAGAAATAAAATTAGTGGTAGGCACACGAAGCG
CGTTATTCTTACCCCTCAAAGAGCTAGGCTTGATTATTGTAGATGAAGAGCATGACTTTTCTTATAAATCCCAGCAAAGC
CCTATGTATAACGCTAGGGATTTATGCTTGTATTTATCCCATAAATTCCCTATTCAAGTGGTTTTAGGCTCTGCTACGCC
AAGTTTGAGTAGCTACAAACGCTTTAAAGATAAGGCTTTAGTGCGCCTAAAGGGGCGCTACATCCCCACACAAAAAAACA
TTATTTTTGAAAAAACCCCAAATTTTATCACGCCAAAACTCCTAGAAGCGTTAAAACAAGTGATAGACAAAAACGAGCAA
GCCATTATTTTTGTGCCTACAAGAGCTAATTTCAAAACCTTGTTGTGTCAAAATTGTTACAAAAGCGTTCAATGCCCCTT
TTGCAGCGTGAATATGAGTTTGCATTTGAGAACTAACAAACTCATGTGCCATTATTGCCATTATTCAAGCCCTATCCCTA
AAATTTGTAGCGCGTGTCAAAGCGAAGTCTTAGTGGGTAAAAGGATAGGCACCATGCAAGTGTTAAAGGAATTAGAAAGC
CTTTTAGAGGGGGTTAAAATAGCGATTTTAGATAAAGATCACACTAGCACGCCAAAAAAACTCCATAATATTTTGAATGA
CTTCAACGCCCAAAAAACCAATATTTTAATCGGCACGCAAATGATAAGCAAAGGGCATGATTACGCTAAAGTGAGTTTAG
CGGTTATTTTAGGCATAGACAATATTATTAAATCTAGTAGTTATAGGGCTTTAGAAGAAGGCGTATCACTGTTGCATCAA
ATCGCCGGAAGGAGCGCGAGACAAATTTCTGGCCAAGTGTTCATTCAAAGCATTGAAGGGGGTTTGTTAGAGAGCTTTTT
AGAAGATTATGAAGATTTTCTACAATACGAATTGCAAGAAAGATGCGAACTCTACCCGCCTTTTTCAAGGCTATGTTTGT
TGGAATTTAAGCATAAAAACGAAGAAAAAGCCCAACAATTAAGCCTAAAAGCTTCTCAAATCCTTTCTTTGTGTTTAGAA
AAGGGCGTAACGCTCTCTAGCTTTAAAGCCCCCATTGAAAAAATCGCTTCCTCTTATCGCTACCTTATTTTATTGCGCTC
CAAAAACCCTTTAAGCCTAGTCAAAAGCGTGCATGCGTTTTTAAAAGCCACCCCCAATATCCCTTGCAGCGTGAATATGG
ATCCTATGGATATTTTTTAA

Upstream 100 bases:

>100_bases
AATTAAACGCATTAGAGCTTTTAAAGGATTATTTACATGAACGTTGTCAAAACGAGTACTTGCACAATGAACTCCAAAAG
CTTTTAGAAAAAATCTCATC

Downstream 100 bases:

>100_bases
AAAATTTAGGTTTTTCTGGCGACTAACAGCGTGAAATTCACCCATTTAAACAACGCTTCCACATGCTTAAACCCCACGCT
TTCTAAAAGAGCGATATTTT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MFYYLIAPLKNKTPPLTYFSKEQHQKGVLVNINLRNKTLLGVILEEVSKPSFECLELEKTPYFLLPIQIELAEFIAQYYS
ASLSLVLSLFAPFKECDVAKLEKIKPNLNGLSQTQTNALNALQKHSASLLFGDTGSGKTEIYMHLIAQMLEQKKSALLLV
PEIALTPQIQQRLKKVFKENLGLWHSKLSLTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSQQS
PMYNARDLCLYLSHKFPIQVVLGSATPSLSSYKRFKDKALVRLKGRYIPTQKNIIFEKTPNFITPKLLEALKQVIDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLRTNKLMCHYCHYSSPIPKICSACQSEVLVGKRIGTMQVLKELES
LLEGVKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVILGIDNIIKSSSYRALEEGVSLLHQ
IAGRSARQISGQVFIQSIEGGLLESFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQILSLCLE
KGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLVKSVHAFLKATPNIPCSVNMDPMDIF

Sequences:

>Translated_619_residues
MFYYLIAPLKNKTPPLTYFSKEQHQKGVLVNINLRNKTLLGVILEEVSKPSFECLELEKTPYFLLPIQIELAEFIAQYYS
ASLSLVLSLFAPFKECDVAKLEKIKPNLNGLSQTQTNALNALQKHSASLLFGDTGSGKTEIYMHLIAQMLEQKKSALLLV
PEIALTPQIQQRLKKVFKENLGLWHSKLSLTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSQQS
PMYNARDLCLYLSHKFPIQVVLGSATPSLSSYKRFKDKALVRLKGRYIPTQKNIIFEKTPNFITPKLLEALKQVIDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLRTNKLMCHYCHYSSPIPKICSACQSEVLVGKRIGTMQVLKELES
LLEGVKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVILGIDNIIKSSSYRALEEGVSLLHQ
IAGRSARQISGQVFIQSIEGGLLESFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQILSLCLE
KGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLVKSVHAFLKATPNIPCSVNMDPMDIF
>Mature_619_residues
MFYYLIAPLKNKTPPLTYFSKEQHQKGVLVNINLRNKTLLGVILEEVSKPSFECLELEKTPYFLLPIQIELAEFIAQYYS
ASLSLVLSLFAPFKECDVAKLEKIKPNLNGLSQTQTNALNALQKHSASLLFGDTGSGKTEIYMHLIAQMLEQKKSALLLV
PEIALTPQIQQRLKKVFKENLGLWHSKLSLTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSQQS
PMYNARDLCLYLSHKFPIQVVLGSATPSLSSYKRFKDKALVRLKGRYIPTQKNIIFEKTPNFITPKLLEALKQVIDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLRTNKLMCHYCHYSSPIPKICSACQSEVLVGKRIGTMQVLKELES
LLEGVKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVILGIDNIIKSSSYRALEEGVSLLHQ
IAGRSARQISGQVFIQSIEGGLLESFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQILSLCLE
KGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLVKSVHAFLKATPNIPCSVNMDPMDIF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=554, Percent_Identity=33.5740072202166, Blast_Score=287, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 70179; Mature: 70179

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYYLIAPLKNKTPPLTYFSKEQHQKGVLVNINLRNKTLLGVILEEVSKPSFECLELEKT
CEEEEEECCCCCCCCCCCCCCHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCC
PYFLLPIQIELAEFIAQYYSASLSLVLSLFAPFKECDVAKLEKIKPNLNGLSQTQTNALN
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHCCCCCCCCHHHHHHHHH
ALQKHSASLLFGDTGSGKTEIYMHLIAQMLEQKKSALLLVPEIALTPQIQQRLKKVFKEN
HHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHCCHHHHHHHHHHHHHH
LGLWHSKLSLTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSQQS
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHCCEEEEECCCCCCCCCCCC
PMYNARDLCLYLSHKFPIQVVLGSATPSLSSYKRFKDKALVRLKGRYIPTQKNIIFEKTP
CCCCHHHEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEECCC
NFITPKLLEALKQVIDKNEQAIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLRTNK
CCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECEEEEEEECC
LMCHYCHYSSPIPKICSACQSEVLVGKRIGTMQVLKELESLLEGVKIAILDKDHTSTPKK
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
LHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVILGIDNIIKSSSYRALEEGVSLLHQ
HHHHHHHCCCCCCEEEEEHHHHHCCCCHHHEEEHHEECHHHHHHCCCHHHHHHHHHHHHH
IAGRSARQISGQVFIQSIEGGLLESFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKN
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
EEKAQQLSLKASQILSLCLEKGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLVKSVHAF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
LKATPNIPCSVNMDPMDIF
HHCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MFYYLIAPLKNKTPPLTYFSKEQHQKGVLVNINLRNKTLLGVILEEVSKPSFECLELEKT
CEEEEEECCCCCCCCCCCCCCHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCC
PYFLLPIQIELAEFIAQYYSASLSLVLSLFAPFKECDVAKLEKIKPNLNGLSQTQTNALN
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHCCCCCCCCHHHHHHHHH
ALQKHSASLLFGDTGSGKTEIYMHLIAQMLEQKKSALLLVPEIALTPQIQQRLKKVFKEN
HHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHCCHHHHHHHHHHHHHH
LGLWHSKLSLTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSQQS
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHCCEEEEECCCCCCCCCCCC
PMYNARDLCLYLSHKFPIQVVLGSATPSLSSYKRFKDKALVRLKGRYIPTQKNIIFEKTP
CCCCHHHEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEECCC
NFITPKLLEALKQVIDKNEQAIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLRTNK
CCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECEEEEEEECC
LMCHYCHYSSPIPKICSACQSEVLVGKRIGTMQVLKELESLLEGVKIAILDKDHTSTPKK
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
LHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVILGIDNIIKSSSYRALEEGVSLLHQ
HHHHHHHCCCCCCEEEEEHHHHHCCCCHHHEEEHHEECHHHHHHCCCHHHHHHHHHHHHH
IAGRSARQISGQVFIQSIEGGLLESFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKN
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
EEKAQQLSLKASQILSLCLEKGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLVKSVHAF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
LKATPNIPCSVNMDPMDIF
HHCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]