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The map label for this gene is ydiS [H]

Identifier: 109946970

GI number: 109946970

Start: 306660

End: 308003

Strand: Direct

Name: ydiS [H]

Synonym: Hac_0362

Alternate gene names: 109946970

Gene position: 306660-308003 (Clockwise)

Preceding gene: 109946969

Following gene: 109946971

Centisome position: 19.73

GC content: 30.65

Gene sequence:

>1344_bases
ATGAAAATTTATTTAAGGAAAGTTGACAATCAATGTATTGTCAAACAAATCAGCATTGCAAGAGGTATTTTAGAACATTT
TTTTGATAACGCCAACGACAAAGATGGAGTAAATATGAAAGGCGTGCTGTCTGGTTATAGTGATAAGGTTAGCATTCTTT
TAGCAACAGATCCTAGACTTGGTGGAGGAATTAAGAAAATTATCAGTGCGGAAGTCAATCAGCTAAAAGAACACAGATTA
GACCGTGAATTAAAAATTGATGATATTCTACTTTTTACTTGCATATCTTCCAAAGAATACACATTAGAAATCATTTTACC
AACTGATACAAAATATAATATTTTAAATAGTTTAATAAACAATAGTAGACATTTATTGGTTTTTAGTGAGAATGAAACAA
GATCTTTAGATGATAAAATGGACGAAAGCATTAGAATAGATGGAGGAAAAAATATAATCCTTTATGGTGTTCCTGGTAGC
GGCAAAAGCTACGCTTTGCAAAGAGATTATTGCAATGATAACAGCGTGGTAGAAAAGATAGTGTTCCATCCTGACTATTC
TTATAGTGATTTTGTGGGACAAATTATGCCGAGTATAGATGATAGCGGTGTTGTAAGCTATAAATTTAATCCTGGACCAT
TTACAAACATTCTTAAAAAGGCATATCATAACCCACAAACTAAGCATATTTTAGTGATTGATGAAATAAATCGTGGTAAT
GCGCCTGCCATATTTGGAGAAATTTTTCAGCTTCTTGACAGACTTAAACATGACAAAGATGGTTTTAAAAAAGGCTCAAG
CGAGTATGCTATCAATAATATGGATATTGCAAAGGTTGTATATAATGATAAAGACGCAAAAATAAGGATCCCATCAAATT
TATGGATAATAGCCACAATGAACACGAGCGATCAAAATGTATTCACGCTAGATACTGCCTTTCAGCGTAGATTTTCTATG
CAACTTATTGAAAATTCCTTTGAAAATGTCAGTGATGATTTTAAAAATATGAAAATTTTAGATACTGATATTACTTGGCA
AAAATTTTGCACCACTATGAATGAAAAAATAGCTCAAAATAACGAAGGACTGAACTCTATGGAAGACAAGCGATTTGGCG
TTTATTTTGTCAATATTGATGATTTAAAGAATAAAGAAAATTTTGCGCACAAAGTCATAAAATACCTTTGGGACGATGTG
TTTAAATTTGATAGAAATATAATATTTAATACTATAAAGTATAATACACTTGAAGCTGTTGTAAAGAATTTTACCAAAGA
AAAAGGTAGAACCCAATTTGATATTTTTCATGATGAGATAAAAGAACTTTTATTCAATGTTTAA

Upstream 100 bases:

>100_bases
CGTCAAATTTGGAGGAGCAAAACATTCCCAGGATTTGCAAAAGCGATGGCAGAGCAGTGGGGGGAGTTTGTAAAAAATGA
GATGTTTAAAAAGGTGAAAA

Downstream 100 bases:

>100_bases
TTTAAAAGCGTGTTGTTTTACAGACAATGAATTGAATGATGATTTTGTAGGTGTTAGAAGCATCAATAATGACTTGCAAA
TTTGTTTCCCACTAGGATTT

Product: type II restriction enzyme

Products: NA

Alternate protein names: R.BsuM; R.BsuMI; Endonuclease BsuMI component ydiS; Type II restriction enzyme BsuMI component ydiS [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKIYLRKVDNQCIVKQISIARGILEHFFDNANDKDGVNMKGVLSGYSDKVSILLATDPRLGGGIKKIISAEVNQLKEHRL
DRELKIDDILLFTCISSKEYTLEIILPTDTKYNILNSLINNSRHLLVFSENETRSLDDKMDESIRIDGGKNIILYGVPGS
GKSYALQRDYCNDNSVVEKIVFHPDYSYSDFVGQIMPSIDDSGVVSYKFNPGPFTNILKKAYHNPQTKHILVIDEINRGN
APAIFGEIFQLLDRLKHDKDGFKKGSSEYAINNMDIAKVVYNDKDAKIRIPSNLWIIATMNTSDQNVFTLDTAFQRRFSM
QLIENSFENVSDDFKNMKILDTDITWQKFCTTMNEKIAQNNEGLNSMEDKRFGVYFVNIDDLKNKENFAHKVIKYLWDDV
FKFDRNIIFNTIKYNTLEAVVKNFTKEKGRTQFDIFHDEIKELLFNV

Sequences:

>Translated_447_residues
MKIYLRKVDNQCIVKQISIARGILEHFFDNANDKDGVNMKGVLSGYSDKVSILLATDPRLGGGIKKIISAEVNQLKEHRL
DRELKIDDILLFTCISSKEYTLEIILPTDTKYNILNSLINNSRHLLVFSENETRSLDDKMDESIRIDGGKNIILYGVPGS
GKSYALQRDYCNDNSVVEKIVFHPDYSYSDFVGQIMPSIDDSGVVSYKFNPGPFTNILKKAYHNPQTKHILVIDEINRGN
APAIFGEIFQLLDRLKHDKDGFKKGSSEYAINNMDIAKVVYNDKDAKIRIPSNLWIIATMNTSDQNVFTLDTAFQRRFSM
QLIENSFENVSDDFKNMKILDTDITWQKFCTTMNEKIAQNNEGLNSMEDKRFGVYFVNIDDLKNKENFAHKVIKYLWDDV
FKFDRNIIFNTIKYNTLEAVVKNFTKEKGRTQFDIFHDEIKELLFNV
>Mature_447_residues
MKIYLRKVDNQCIVKQISIARGILEHFFDNANDKDGVNMKGVLSGYSDKVSILLATDPRLGGGIKKIISAEVNQLKEHRL
DRELKIDDILLFTCISSKEYTLEIILPTDTKYNILNSLINNSRHLLVFSENETRSLDDKMDESIRIDGGKNIILYGVPGS
GKSYALQRDYCNDNSVVEKIVFHPDYSYSDFVGQIMPSIDDSGVVSYKFNPGPFTNILKKAYHNPQTKHILVIDEINRGN
APAIFGEIFQLLDRLKHDKDGFKKGSSEYAINNMDIAKVVYNDKDAKIRIPSNLWIIATMNTSDQNVFTLDTAFQRRFSM
QLIENSFENVSDDFKNMKILDTDITWQKFCTTMNEKIAQNNEGLNSMEDKRFGVYFVNIDDLKNKENFAHKVIKYLWDDV
FKFDRNIIFNTIKYNTLEAVVKNFTKEKGRTQFDIFHDEIKELLFNV

Specific function: Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown [H]

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=266, Percent_Identity=31.203007518797, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011704 [H]

Pfam domain/function: PF07728 AAA_5 [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 51530; Mature: 51530

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLRKVDNQCIVKQISIARGILEHFFDNANDKDGVNMKGVLSGYSDKVSILLATDPRL
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEECCCC
GGGIKKIISAEVNQLKEHRLDRELKIDDILLFTCISSKEYTLEIILPTDTKYNILNSLIN
CCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHHHC
NSRHLLVFSENETRSLDDKMDESIRIDGGKNIILYGVPGSGKSYALQRDYCNDNSVVEKI
CCCEEEEEECCCCCCHHHHCCCCEEECCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHE
VFHPDYSYSDFVGQIMPSIDDSGVVSYKFNPGPFTNILKKAYHNPQTKHILVIDEINRGN
EECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
APAIFGEIFQLLDRLKHDKDGFKKGSSEYAINNMDIAKVVYNDKDAKIRIPSNLWIIATM
CCHHHHHHHHHHHHHHCCHHHHHCCCCCEEECCCEEEEEEECCCCCEEEECCCEEEEEEE
NTSDQNVFTLDTAFQRRFSMQLIENSFENVSDDFKNMKILDTDITWQKFCTTMNEKIAQN
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCC
NEGLNSMEDKRFGVYFVNIDDLKNKENFAHKVIKYLWDDVFKFDRNIIFNTIKYNTLEAV
CCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
VKNFTKEKGRTQFDIFHDEIKELLFNV
HHHHHHHCCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIYLRKVDNQCIVKQISIARGILEHFFDNANDKDGVNMKGVLSGYSDKVSILLATDPRL
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEECCCC
GGGIKKIISAEVNQLKEHRLDRELKIDDILLFTCISSKEYTLEIILPTDTKYNILNSLIN
CCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHHHC
NSRHLLVFSENETRSLDDKMDESIRIDGGKNIILYGVPGSGKSYALQRDYCNDNSVVEKI
CCCEEEEEECCCCCCHHHHCCCCEEECCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHE
VFHPDYSYSDFVGQIMPSIDDSGVVSYKFNPGPFTNILKKAYHNPQTKHILVIDEINRGN
EECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
APAIFGEIFQLLDRLKHDKDGFKKGSSEYAINNMDIAKVVYNDKDAKIRIPSNLWIIATM
CCHHHHHHHHHHHHHHCCHHHHHCCCCCEEECCCEEEEEEECCCCCEEEECCCEEEEEEE
NTSDQNVFTLDTAFQRRFSMQLIENSFENVSDDFKNMKILDTDITWQKFCTTMNEKIAQN
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCC
NEGLNSMEDKRFGVYFVNIDDLKNKENFAHKVIKYLWDDVFKFDRNIIFNTIKYNTLEAV
CCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
VKNFTKEKGRTQFDIFHDEIKELLFNV
HHHHHHHCCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]