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The map label for this gene is leuS

Identifier: 109946907

GI number: 109946907

Start: 245972

End: 248392

Strand: Direct

Name: leuS

Synonym: Hac_0293

Alternate gene names: 109946907

Gene position: 245972-248392 (Clockwise)

Preceding gene: 109946906

Following gene: 109946908

Centisome position: 15.83

GC content: 39.24

Gene sequence:

>2421_bases
ATGGATTTTGTGAGTATAGAAAAAAAATGGCAAGAATTTTGGCACCAAAATGAGAGTTTTGAACCTAAAGACGATTTTAA
CCTCCCTAAAAAATACATTTTAAGCATGCTGCCTTATCCTAGTGGGGAAATCCACATGGGGCATGTGCGCAATTACACCA
TTGGCGATGCTTTGGTGCGCTATTATCGTTTGCACCATTACAATGTATTACACCCTATGGGGTTTGATTCTTTTGGGATG
CCTGCAGAAAACGCTGCCATTAAGCATGGTATCCACCCTAAAACCTGGACTTATGAAAATATTGAAAACATGCAAAAAGA
GTTTGAAGCTCTAGGGTTTTCTTTTTCTAAAAATAGGGAATTTGCCACTTCAGATCCCAATTACACGGAATTTGAACAAC
AATTTTTCATTGACTTGTGGGAAAAAGGATTGATTTATCGTAAAAAGGCCATGCTCAATTGGTGTCCTAACGATAAAACC
GTTTTAGCTAACGAGCAAGTCATAGAGGGGAGGTGCTGGCGTTGCGATACAGAAGTCGTTCAAAAAGAACTCTACCAATA
TTATTTAAAAATCACAAACTACGCTGAAGAATTGTTAAAAGACTTAGAAACTTTAAAAAATCATTGGCCTTCTCAAGTCT
TGCTCATGCAAAAAAATTGGATAGGGAAATCTAGCGGGTTGCAATTTCGTTTTAAAATCGCTGATGAGTGCTTGAAAGCT
TGCAATGACATTCAAGAAATTGAAGTCTTTACCACAAGAGCGGATACCATTTATGGCGTAACTTACATTGCAATCGCCCC
AGAACACCCTTTAGTAGAGCATGCCATCAAGCAAGTGAATCAAGAGGATTTAAAGACTATTAAAGCGATTTTAAACACGA
CTCCAAGAGAAAGAACTTTGGAGAAAAAGGGGGCGTTTTTAGGCATTTACGCTATCCACCCTTTAACGAAGCAAAAAATC
CCTATTTGGGTGGCCAATTTTGCATTAGCCAATTACGGCTCTGGGGCGTTAATGGGCGTGCCTGCTTGCGATGAAAGGGA
TTTTGAATTCGCTAATCTTTATCATATCCCTATCAAAGTGATCACTCAAAGCCCTCAAAATTTGCCCCACACCAAAGAAG
AAACTTTAAAAAATAGTGGGGAGTGGAGCGACCTTCCTAGCTCAGTGGCTAGAGAAAAAATCATCGCTTATTTTGAAAAA
GAAAATCTCGGTAAAAGAGTGATAAACTACCGCTTGCAAGATTGGGGGGTAAGCCGTCAAAGGTATTGGGGAGCGCCCAT
TCCTATGATTCATTGTAAGAATTGCGGGATCGTGCCTGAAACCCAACTACCGGTAACTTTACCCGAAGATATTGTGATTG
ATGGGGAGGGCAATCCTTTAGAAAAGCATGCCAGTTGGAAATTCACTCAATGCCCTAAATGCCACAAAGACGCTTTAAGA
GAAACAGATACCATGGATACTTTCATTCAGTCTAGCTGGTATTTCTTGCGCTACACCACCCCTAAAAATCAGCGTGAAAA
TCAAGCGTTTGATAAAAACTACTTGAAGTATTTCATGCCAGTGGATACTTATATTGGTGGCATTGAACATGCGATTTTAC
ACTTATTATACGCGCGCTTTTTCACTAAGGCTTTAAGGGATTTGGGCTATATTGATTTAGATGAGCCTTTCAAACAGCTT
ATCACTCAAGGCATGGTCTTAAAAGATGGCGTTAAAATGAGCAAGTCTAAAGGCAATGTCGTTAGCCCTAAAGAAATACT
CAAAAAATATGGGGCGGATGCGGCAAGGCTTTTTATCCTTTTTGCTGCCCCACCGGCTAAAGAATTGGAATGGAATGACA
GTGCTTTAGAGGGGGCACATCGGTTTATCAAGCGCTTATACGACAAAGCGAACGCTATTAACCCAACCACTTATAAGCCT
GAATTTAAAGAAGCGAACTTGAATGAAGCGGAAAAATTAGCTCGCAAAAAAGTCTATGAGGCGTTGAAAAAATCGCATGA
AATTTTCAATAATCCTGAAAGCACTTACGCGTTTAACACTTTGATCGCAAGTTGCATGGAAGCTTTAAACGCTTTAAATA
CGCAAAATAATGAGCGAATTTTATGCGAAGGCTATTTTGTGTTATTACAAGTTTTAGAGCCTATGATCCCACACACCGCA
TGGGAATTGAGCGAAAGGCTTTTTAAAAGAGCGAATTTTAAGCCTATAACAATAGATGAGAGCGCTTTAATAGAAGACTC
TATGACTTTAGCACTCACGATCAATGGCAAAAGGCGTGCGGAATTGAAAGTCCATATTAACGCCAGTAAAGAAGAAATTC
TCGCTTTGGCTAAAAAAGAATTGGAGAAATATTTAGAAAATGCGAGCGTTAAAAAAGAAATTTATGTGCCTAATAAGCTT
GTTAATTTTGTTATCGCATGA

Upstream 100 bases:

>100_bases
TTACATGCTCATGATTGGGGCGTTGATTAGTAACACTTTTAGTTTGATCTTTTTATTGATTGAAAGCGTTGTAACGAGTG
AAACAGACTAAGGGATAGCG

Downstream 100 bases:

>100_bases
TTTTTAGGGCTTTATTTTTCTTTATTGTGTTGTTTTTGTTAAAGGGCTGTGGGTATAAGCCCATCGCTACTTACGCCCAA
AACGCGCTTGGCAATAGCGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MDFVSIEKKWQEFWHQNESFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALVRYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNREFATSDPNYTEFEQQFFIDLWEKGLIYRKKAMLNWCPNDKT
VLANEQVIEGRCWRCDTEVVQKELYQYYLKITNYAEELLKDLETLKNHWPSQVLLMQKNWIGKSSGLQFRFKIADECLKA
CNDIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVNQEDLKTIKAILNTTPRERTLEKKGAFLGIYAIHPLTKQKI
PIWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEETLKNSGEWSDLPSSVAREKIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCKNCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKDALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDKNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYIDLDEPFKQL
ITQGMVLKDGVKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTYKP
EFKEANLNEAEKLARKKVYEALKKSHEIFNNPESTYAFNTLIASCMEALNALNTQNNERILCEGYFVLLQVLEPMIPHTA
WELSERLFKRANFKPITIDESALIEDSMTLALTINGKRRAELKVHINASKEEILALAKKELEKYLENASVKKEIYVPNKL
VNFVIA

Sequences:

>Translated_806_residues
MDFVSIEKKWQEFWHQNESFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALVRYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNREFATSDPNYTEFEQQFFIDLWEKGLIYRKKAMLNWCPNDKT
VLANEQVIEGRCWRCDTEVVQKELYQYYLKITNYAEELLKDLETLKNHWPSQVLLMQKNWIGKSSGLQFRFKIADECLKA
CNDIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVNQEDLKTIKAILNTTPRERTLEKKGAFLGIYAIHPLTKQKI
PIWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEETLKNSGEWSDLPSSVAREKIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCKNCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKDALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDKNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYIDLDEPFKQL
ITQGMVLKDGVKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTYKP
EFKEANLNEAEKLARKKVYEALKKSHEIFNNPESTYAFNTLIASCMEALNALNTQNNERILCEGYFVLLQVLEPMIPHTA
WELSERLFKRANFKPITIDESALIEDSMTLALTINGKRRAELKVHINASKEEILALAKKELEKYLENASVKKEIYVPNKL
VNFVIA
>Mature_806_residues
MDFVSIEKKWQEFWHQNESFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALVRYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNREFATSDPNYTEFEQQFFIDLWEKGLIYRKKAMLNWCPNDKT
VLANEQVIEGRCWRCDTEVVQKELYQYYLKITNYAEELLKDLETLKNHWPSQVLLMQKNWIGKSSGLQFRFKIADECLKA
CNDIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVNQEDLKTIKAILNTTPRERTLEKKGAFLGIYAIHPLTKQKI
PIWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEETLKNSGEWSDLPSSVAREKIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCKNCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKDALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDKNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYIDLDEPFKQL
ITQGMVLKDGVKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTYKP
EFKEANLNEAEKLARKKVYEALKKSHEIFNNPESTYAFNTLIASCMEALNALNTQNNERILCEGYFVLLQVLEPMIPHTA
WELSERLFKRANFKPITIDESALIEDSMTLALTINGKRRAELKVHINASKEEILALAKKELEKYLENASVKKEIYVPNKL
VNFVIA

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=877, Percent_Identity=32.8392246294185, Blast_Score=448, Evalue=1e-126,
Organism=Escherichia coli, GI1786861, Length=871, Percent_Identity=42.2502870264064, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=413, Percent_Identity=23.4866828087167, Blast_Score=93, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71997510, Length=728, Percent_Identity=30.4945054945055, Blast_Score=281, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71997517, Length=728, Percent_Identity=30.4945054945055, Blast_Score=281, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI212645227, Length=271, Percent_Identity=32.4723247232472, Blast_Score=115, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323414, Length=883, Percent_Identity=36.806342015855, Blast_Score=513, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6321531, Length=873, Percent_Identity=20.6185567010309, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355409, Length=666, Percent_Identity=35.7357357357357, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281366294, Length=141, Percent_Identity=29.0780141843972, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_HELAH (Q17YZ0)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664135.1
- ProteinModelPortal:   Q17YZ0
- SMR:   Q17YZ0
- STRING:   Q17YZ0
- GeneID:   4177613
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0293
- NMPDR:   fig|382638.8.peg.282
- eggNOG:   COG0495
- HOGENOM:   HBG693845
- OMA:   YARYCSP
- PhylomeDB:   Q17YZ0
- ProtClustDB:   PRK00390
- BioCyc:   HACI382638:HAC_0293-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 93376; Mature: 93376

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 575-575

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFVSIEKKWQEFWHQNESFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALVR
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
YYRLHHYNVLHPMGFDSFGMPAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNRE
HHHHHHCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
FATSDPNYTEFEQQFFIDLWEKGLIYRKKAMLNWCPNDKTVLANEQVIEGRCWRCDTEVV
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCHHHCCCCCCCCHHHH
QKELYQYYLKITNYAEELLKDLETLKNHWPSQVLLMQKNWIGKSSGLQFRFKIADECLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHH
CNDIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVNQEDLKTIKAILNTTPRERTL
HCCHHHHHEEEECCCEEEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
EKKGAFLGIYAIHPLTKQKIPIWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKV
HHCCCEEEEEEECCCCCCCCCEEEEHHHHHCCCCCCEECCCCCCCCCCCEEEEEEEEEEE
ITQSPQNLPHTKEETLKNSGEWSDLPSSVAREKIIAYFEKENLGKRVINYRLQDWGVSRQ
EECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RYWGAPIPMIHCKNCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKDALR
HCCCCCCCEEEECCCCCCCCCCCCEECCCCEEECCCCCCCHHHCCCCCCCCCHHHHHHHH
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDKNYLKYFMPVDTYIGGIEHAILHLLYARF
HHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FTKALRDLGYIDLDEPFKQLITQGMVLKDGVKMSKSKGNVVSPKEILKKYGADAARLFIL
HHHHHHHCCCCCCCHHHHHHHHCCCEECCCCHHCCCCCCCCCHHHHHHHHCCCCEEEEEE
FAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTYKPEFKEANLNEAEKLARKKVYE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALKKSHEIFNNPESTYAFNTLIASCMEALNALNTQNNERILCEGYFVLLQVLEPMIPHTA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCHHH
WELSERLFKRANFKPITIDESALIEDSMTLALTINGKRRAELKVHINASKEEILALAKKE
HHHHHHHHHHCCCCEEEECHHHHHCCCEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHH
LEKYLENASVKKEIYVPNKLVNFVIA
HHHHHHCCCCCEEEECCHHHHHHHCC
>Mature Secondary Structure
MDFVSIEKKWQEFWHQNESFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALVR
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
YYRLHHYNVLHPMGFDSFGMPAENAAIKHGIHPKTWTYENIENMQKEFEALGFSFSKNRE
HHHHHHCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
FATSDPNYTEFEQQFFIDLWEKGLIYRKKAMLNWCPNDKTVLANEQVIEGRCWRCDTEVV
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCHHHCCCCCCCCHHHH
QKELYQYYLKITNYAEELLKDLETLKNHWPSQVLLMQKNWIGKSSGLQFRFKIADECLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHH
CNDIQEIEVFTTRADTIYGVTYIAIAPEHPLVEHAIKQVNQEDLKTIKAILNTTPRERTL
HCCHHHHHEEEECCCEEEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
EKKGAFLGIYAIHPLTKQKIPIWVANFALANYGSGALMGVPACDERDFEFANLYHIPIKV
HHCCCEEEEEEECCCCCCCCCEEEEHHHHHCCCCCCEECCCCCCCCCCCEEEEEEEEEEE
ITQSPQNLPHTKEETLKNSGEWSDLPSSVAREKIIAYFEKENLGKRVINYRLQDWGVSRQ
EECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RYWGAPIPMIHCKNCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFTQCPKCHKDALR
HCCCCCCCEEEECCCCCCCCCCCCEECCCCEEECCCCCCCHHHCCCCCCCCCHHHHHHHH
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDKNYLKYFMPVDTYIGGIEHAILHLLYARF
HHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FTKALRDLGYIDLDEPFKQLITQGMVLKDGVKMSKSKGNVVSPKEILKKYGADAARLFIL
HHHHHHHCCCCCCCHHHHHHHHCCCEECCCCHHCCCCCCCCCHHHHHHHHCCCCEEEEEE
FAAPPAKELEWNDSALEGAHRFIKRLYDKANAINPTTYKPEFKEANLNEAEKLARKKVYE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALKKSHEIFNNPESTYAFNTLIASCMEALNALNTQNNERILCEGYFVLLQVLEPMIPHTA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCHHH
WELSERLFKRANFKPITIDESALIEDSMTLALTINGKRRAELKVHINASKEEILALAKKE
HHHHHHHHHHCCCCEEEECHHHHHCCCEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHH
LEKYLENASVKKEIYVPNKLVNFVIA
HHHHHHCCCCCEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA