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The map label for this gene is pgk [H]

Identifier: 109946890

GI number: 109946890

Start: 225345

End: 226553

Strand: Reverse

Name: pgk [H]

Synonym: Hac_0274

Alternate gene names: 109946890

Gene position: 226553-225345 (Counterclockwise)

Preceding gene: 109946891

Following gene: 109946889

Centisome position: 14.58

GC content: 40.36

Gene sequence:

>1209_bases
ATGTTAGCTAAAATGTCGTTTATGCAAAATGTTAAAAACATTCAAGAAGTGGATGTCAATCATAAAAAAGTGCTTATTAG
AGTGGATTTTAATGTGCCTTTAGATGAAAATTTGAATATTACTGATGATACGCGCATTAGAGAGAGCTTGCCTACCATTC
AATATTGTATTGACAACAAGGCTAAAGATATTATTTTAGTGAGCCATTTGGGTCGCCCTAAAGGGGTTGAAGAAAAATTG
AGCTTAAAGCCCTTTTTAAAGCGCCTTGAAAGATTGCTTAATCATGAGGTGATTTTTTCTCAGAATATCGTGCAATTGAA
ACAAGCTTTAAACGAAAACGCACCCACAAGGATCTTTCTTTTAGAAAATATCCGCTTTTTAAAAGGCGAAGAAGAAAATG
ATGAAAACTTGGCTAAAGATTTAGCAAGTTTGTGCGATGTGTTTGTGAATGACGCTTTTGGCACTAGCCACAGAAAGCAT
GCGAGTACTTATGGCACCGCTAAATTCGCTCCCATTAAAGTGAGCGGGTTTTTACTCAAAAAAGAAATTGATTCGTTTTA
TCAAGCGTTTAACCACCCCTTACGCCCCTTATTGTTGATTGTAGGGGGGGCTAAGGTTAGCTCCAAACTCACCTTATTAA
AAAACATTTTAGATCTCATTGACAAGCTCATTATTGCCGGAGCAATGAGTAACACTTTCTTAAAAGCCTTAGGCTATGAT
GTGCAAGATTCTTTGGTGGAAGACTCCTTACTAAATGACGCCTTGGAGCTGTTAAAAAGCGCGAAAGAAAAAAAAGTCAA
AGTCTATTTACCCATAGACGCTGTCACTACCGATGACATTCTTAACCCCAAGCACATTAAAATCTCGCCCGTCCAAGACA
TTGAGCCCAAGCACAAAATCGCTGACATAGGGCCTGCGAGCGTGAAATTGTTCTCTGAAGTCATAGAGAGTGCACCCACG
ATCATATGGAATGGCCCTTTAGGCGTGCATGAAAAACAAGAATTCGCTAGAGGCACGACCTTTTTAGCCCACAAAATCGC
TGACACTTACGCCTTTTCGCTCGTGGGTGGGGGCGATACCATTGATGCGATCAACCGCGTAGGCGAAAAGGATAACATGA
GCTTTATTTCCACCGGTGGAGGGGCGAGTTTGGAATTATTGGAGGGCAAAATTTTACCTTGTTTTGAGGTTTTGGATAAA
CGCCATTGA

Upstream 100 bases:

>100_bases
ATGCTAAAGTATTGGCATGGTATGATAATGAAATAGGTTATAGCGAGCGCTTGATAGACATGGTGCAATATATAACGCAA
AATTAAAGAAAAGGATTTTT

Downstream 100 bases:

>100_bases
CTTTAAGGATCACTTATGGTGAATGTGTTTTTCAAGCAGCAAAAATTTGTCATTAAGAAACGCTTTAACGATTTTAATGG
TTTTGATATAGAAGAAAATG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLAKMSFMQNVKNIQEVDVNHKKVLIRVDFNVPLDENLNITDDTRIRESLPTIQYCIDNKAKDIILVSHLGRPKGVEEKL
SLKPFLKRLERLLNHEVIFSQNIVQLKQALNENAPTRIFLLENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKH
ASTYGTAKFAPIKVSGFLLKKEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
VQDSLVEDSLLNDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKIADIGPASVKLFSEVIESAPT
IIWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLVGGGDTIDAINRVGEKDNMSFISTGGGASLELLEGKILPCFEVLDK
RH

Sequences:

>Translated_402_residues
MLAKMSFMQNVKNIQEVDVNHKKVLIRVDFNVPLDENLNITDDTRIRESLPTIQYCIDNKAKDIILVSHLGRPKGVEEKL
SLKPFLKRLERLLNHEVIFSQNIVQLKQALNENAPTRIFLLENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKH
ASTYGTAKFAPIKVSGFLLKKEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
VQDSLVEDSLLNDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKIADIGPASVKLFSEVIESAPT
IIWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLVGGGDTIDAINRVGEKDNMSFISTGGGASLELLEGKILPCFEVLDK
RH
>Mature_402_residues
MLAKMSFMQNVKNIQEVDVNHKKVLIRVDFNVPLDENLNITDDTRIRESLPTIQYCIDNKAKDIILVSHLGRPKGVEEKL
SLKPFLKRLERLLNHEVIFSQNIVQLKQALNENAPTRIFLLENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKH
ASTYGTAKFAPIKVSGFLLKKEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
VQDSLVEDSLLNDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKIADIGPASVKLFSEVIESAPT
IIWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLVGGGDTIDAINRVGEKDNMSFISTGGGASLELLEGKILPCFEVLDK
RH

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=41.5647921760391, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=39.5180722891566, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=41.8781725888325, Blast_Score=306, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17508823, Length=408, Percent_Identity=40.6862745098039, Blast_Score=295, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6319857, Length=411, Percent_Identity=39.9026763990268, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=38.9830508474576, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI17136394, Length=417, Percent_Identity=39.0887290167866, Blast_Score=254, Evalue=9e-68,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 44785; Mature: 44785

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKMSFMQNVKNIQEVDVNHKKVLIRVDFNVPLDENLNITDDTRIRESLPTIQYCIDNK
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHCCHHHHHHCCC
AKDIILVSHLGRPKGVEEKLSLKPFLKRLERLLNHEVIFSQNIVQLKQALNENAPTRIFL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEE
LENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKHASTYGTAKFAPIKVSGFLLK
ECCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEECEEEEHHHHHH
KEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VQDSLVEDSLLNDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKI
HHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHH
ADIGPASVKLFSEVIESAPTIIWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLVGGGDT
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCH
IDAINRVGEKDNMSFISTGGGASLELLEGKILPCFEVLDKRH
HHHHHHCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MLAKMSFMQNVKNIQEVDVNHKKVLIRVDFNVPLDENLNITDDTRIRESLPTIQYCIDNK
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHCCHHHHHHCCC
AKDIILVSHLGRPKGVEEKLSLKPFLKRLERLLNHEVIFSQNIVQLKQALNENAPTRIFL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEE
LENIRFLKGEEENDENLAKDLASLCDVFVNDAFGTSHRKHASTYGTAKFAPIKVSGFLLK
ECCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEECEEEEHHHHHH
KEIDSFYQAFNHPLRPLLLIVGGAKVSSKLTLLKNILDLIDKLIIAGAMSNTFLKALGYD
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VQDSLVEDSLLNDALELLKSAKEKKVKVYLPIDAVTTDDILNPKHIKISPVQDIEPKHKI
HHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHH
ADIGPASVKLFSEVIESAPTIIWNGPLGVHEKQEFARGTTFLAHKIADTYAFSLVGGGDT
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCH
IDAINRVGEKDNMSFISTGGGASLELLEGKILPCFEVLDKRH
HHHHHHCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]