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The map label for this gene is nuoI

Identifier: 109946837

GI number: 109946837

Start: 186141

End: 186803

Strand: Reverse

Name: nuoI

Synonym: Hac_0216

Alternate gene names: 109946837

Gene position: 186803-186141 (Counterclockwise)

Preceding gene: 109946838

Following gene: 109946836

Centisome position: 12.02

GC content: 43.89

Gene sequence:

>663_bases
ATGGCTAAACAAGAATACAAACAACTTCCTAAACGAGCCGAAATCCATAGCGCGACAGAGCAATTTAAAGACACCATTAA
AACAAGCTTGGGTTTGGATCTATTCAAAGGGCTAGGCCTTACGATCAAGGAATTTTTTAGCCCTAGCGTAACCATCCATT
ATCCCATGGAGCAACTCCCTTTAAGCCCACGCTATCGTGCGGTGCATAATTTGCAACGGCTTTTAGACTCAGGCTCTGAA
AGGTGTATTGGCTGTGGGTTGTGCGAAAAGATTTGCACGAGCAATTGCATAAGGATCATCACGCATAAGGGCGAAGACAA
CCGCAAAAAGATTGATTCTTACACAATCAATTTGGGGCGCTGTATTTATTGCGGATTGTGTGCAGAAGTTTGCCCCGAAT
TAGCGATCGTGATGGGGAATCGTTTTGAAAACGCTAGTACCCAACGCTCCCAATACGGATCTAAAAGCGAGTTTTTAACG
AGCGAGCAAGACGCTAAAGATTGCTCGCATGCCGAGTTTTTAGGCTTTGGTTTTGTAAGCCCTAACTATAACGAACGCAT
GCAAGCCACCCCTTTAGATTATGTCCAAGAGCCTTCAAAAGAAGAATCAAAAAAAGAAACTCCAACAAGCCCAGAGGCCA
ACAAGGGAGATGAAAATGTTTGA

Upstream 100 bases:

>100_bases
CCCAGACCAACTGATGAACATGTGTTGGAAAATCATGTTGCCTTTAGCGCTATTGAACATTGTGCTAACAGGCGTTATTA
TTTTAATTTAAGGAGGTTTT

Downstream 100 bases:

>100_bases
AACCATTGCCTTTTATTTCTTTGCGATCCTTACTTTGAGCATGGCGTTAGTGGTGATCACGACCACAAACATTCTCTATG
CCATTACCGCTCTTGCTAGT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKQEYKQLPKRAEIHSATEQFKDTIKTSLGLDLFKGLGLTIKEFFSPSVTIHYPMEQLPLSPRYRAVHNLQRLLDSGSE
RCIGCGLCEKICTSNCIRIITHKGEDNRKKIDSYTINLGRCIYCGLCAEVCPELAIVMGNRFENASTQRSQYGSKSEFLT
SEQDAKDCSHAEFLGFGFVSPNYNERMQATPLDYVQEPSKEESKKETPTSPEANKGDENV

Sequences:

>Translated_220_residues
MAKQEYKQLPKRAEIHSATEQFKDTIKTSLGLDLFKGLGLTIKEFFSPSVTIHYPMEQLPLSPRYRAVHNLQRLLDSGSE
RCIGCGLCEKICTSNCIRIITHKGEDNRKKIDSYTINLGRCIYCGLCAEVCPELAIVMGNRFENASTQRSQYGSKSEFLT
SEQDAKDCSHAEFLGFGFVSPNYNERMQATPLDYVQEPSKEESKKETPTSPEANKGDENV
>Mature_219_residues
AKQEYKQLPKRAEIHSATEQFKDTIKTSLGLDLFKGLGLTIKEFFSPSVTIHYPMEQLPLSPRYRAVHNLQRLLDSGSER
CIGCGLCEKICTSNCIRIITHKGEDNRKKIDSYTINLGRCIYCGLCAEVCPELAIVMGNRFENASTQRSQYGSKSEFLTS
EQDAKDCSHAEFLGFGFVSPNYNERMQATPLDYVQEPSKEESKKETPTSPEANKGDENV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=138, Percent_Identity=42.0289855072464, Blast_Score=114, Evalue=5e-26,
Organism=Escherichia coli, GI1788617, Length=124, Percent_Identity=36.2903225806452, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555194, Length=114, Percent_Identity=44.7368421052632, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17864306, Length=111, Percent_Identity=45.045045045045, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_HELAH (Q17Z60)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664065.1
- ProteinModelPortal:   Q17Z60
- SMR:   Q17Z60
- STRING:   Q17Z60
- GeneID:   4176455
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0216
- NMPDR:   fig|382638.8.peg.210
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   NCIRIIT
- PhylomeDB:   Q17Z60
- ProtClustDB:   PRK05888
- BioCyc:   HACI382638:HAC_0216-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 24776; Mature: 24645

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQEYKQLPKRAEIHSATEQFKDTIKTSLGLDLFKGLGLTIKEFFSPSVTIHYPMEQLP
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEECCHHHCC
LSPRYRAVHNLQRLLDSGSERCIGCGLCEKICTSNCIRIITHKGEDNRKKIDSYTINLGR
CCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHEECHHH
CIYCGLCAEVCPELAIVMGNRFENASTQRSQYGSKSEFLTSEQDAKDCSHAEFLGFGFVS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCHHHHHCCHHHHHCCCCCC
PNYNERMQATPLDYVQEPSKEESKKETPTSPEANKGDENV
CCCCCCCCCCCHHHHHCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKQEYKQLPKRAEIHSATEQFKDTIKTSLGLDLFKGLGLTIKEFFSPSVTIHYPMEQLP
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEECCHHHCC
LSPRYRAVHNLQRLLDSGSERCIGCGLCEKICTSNCIRIITHKGEDNRKKIDSYTINLGR
CCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHEECHHH
CIYCGLCAEVCPELAIVMGNRFENASTQRSQYGSKSEFLTSEQDAKDCSHAEFLGFGFVS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCHHHHHCCHHHHHCCCCCC
PNYNERMQATPLDYVQEPSKEESKKETPTSPEANKGDENV
CCCCCCCCCCCHHHHHCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA