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The map label for this gene is dnaB [H]

Identifier: 109946815

GI number: 109946815

Start: 162000

End: 163460

Strand: Direct

Name: dnaB [H]

Synonym: Hac_0193

Alternate gene names: 109946815

Gene position: 162000-163460 (Clockwise)

Preceding gene: 109946814

Following gene: 109946816

Centisome position: 10.43

GC content: 39.22

Gene sequence:

>1461_bases
ATGAATAGTGCAGAACAATTGCAACGATTGCTAGAAATTGAAAGGATCGTGCTTTCAGGCATTGTGTTTGCGAATCATAA
GATTGAAGAGATCCATAGCGTTTTAGAGCCTAGCGATTTTTACTATCCGCCCCATAGATTGTTTTTTGAAATCGCTTTTA
AACTCCATGAAGAAAATTACCCTGTTGATGAGAATTTTATCCGCCAAAAAATGCCTAAAGACAAGCAAATCAATGAAGAA
GATCTAGTCGCTATTTTTGCTGCAAGCCCTATAGACAATATTGAAGCCTATGTGGAAGAGATTAAAAACGCCTCCATTAA
ACGAAAACTTCTTGCTTTAGCCAACACCCTTAGAGAGCAATCCCTAGAAAACTCGCAAAAATCTAGCGATATTTTAAACG
CCATAGAGCGAGAAGTCTATACGCTTTTAAATGGCAGCACGATAGAGGGCTTTAGGGACATTCAAGAAGTGCTTGCAAGC
ACGATGAATATTATTACAGAAAACCAAAAAAAAGGGAGTTTAGAAGTTACCGGCATACCGACCGGCTTTGCCCAATTAGA
TAATTACACGAGTGGTTTTAATAAGGGGAGTTTAGTCATTATAGGTGCAAGGCCGTCTATGGGTAAGACTAGTTTGATGA
TGAACATGGTCTTATCTGCACTCAATGACGATAAAGGGGTAGCGGTTTTTAGCTTGGAAATGTCAGCGGAGCAACTCGCT
TTAAGGGCGTTATCAGGTCTCACTTCCATTAACATGCATGATTTAGAGAGCGGGAGGCTTGATGATGATCAGTGGGAAAA
TTTAGCCAAATGTTTTGATGATATTTCTAGGAAAAAACTTTTCTTCTATGATAAAAGTTATTTAAGAATAAATCAAGTCC
GCTTGCAGCTACGAAAGCTTAAATCCCAACACAAGGAATTAGGTATCGCTTTTATTGACTATTTGCAACTCATGTCAGGG
AGCAAAGCCAATACAGAACGCCATGAGCAAATCGCTGAAATTTCAAGGGAGCTTAAAACTTTAGCCAGAGAATTGGAAAT
TCCTATTATAGCGTTAGTCCAGCTTAACCGCAGCCTAGAAAACAGAGACGATAAGCGCCCCATTCTTTCGGATATTAAAG
ACAGTGGGGGGATTGAACAAGATGCGGATATTGTCTTATTTTTGTATAGAGGCTATATCTATCAAATGAGGGCTGAAGAC
AACAAAATAGACAAGCTTAAAAAAGAAGGCAAGATTGAAGAGGCACAAGAGTTGCGTTTAAAGGTCAATGAAGAAAGGCG
TATCCACAAACAAAATGGCAGCATTGAAGAGGCTGAAATCATTGTAGCTAAAAACAGGAATGGGGCTACAGGAACGGTAT
ATACGCGCTTTAACGCTCCTTTCACGCGCTATGAAGACATGCCTATAGATTCTCATTTAGAAGAAGGGCAAGAAACTAAA
TTTGAAATACCCATAACTTGA

Upstream 100 bases:

>100_bases
GCTTAGCGCATGCTATAGCGAGTTTAGAGTTTAAGAATCATTACGCTTTAACGCCATTGGATTTGATAGAAAAGATTAAA
TTATTATAAAAGGATAGATA

Downstream 100 bases:

>100_bases
AAGATAAAACCTTTCAAGGGGCGTTTGATATTCTTTCAAGCCCCAAAGAATACTTATGGTGTGGGGTTGTTTTAAGCCTT
TTGTTTGTCATTAACCTCTA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MNSAEQLQRLLEIERIVLSGIVFANHKIEEIHSVLEPSDFYYPPHRLFFEIAFKLHEENYPVDENFIRQKMPKDKQINEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLLALANTLREQSLENSQKSSDILNAIEREVYTLLNGSTIEGFRDIQEVLAS
TMNIITENQKKGSLEVTGIPTGFAQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDKGVAVFSLEMSAEQLA
LRALSGLTSINMHDLESGRLDDDQWENLAKCFDDISRKKLFFYDKSYLRINQVRLQLRKLKSQHKELGIAFIDYLQLMSG
SKANTERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEAQELRLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEEGQETK
FEIPIT

Sequences:

>Translated_486_residues
MNSAEQLQRLLEIERIVLSGIVFANHKIEEIHSVLEPSDFYYPPHRLFFEIAFKLHEENYPVDENFIRQKMPKDKQINEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLLALANTLREQSLENSQKSSDILNAIEREVYTLLNGSTIEGFRDIQEVLAS
TMNIITENQKKGSLEVTGIPTGFAQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDKGVAVFSLEMSAEQLA
LRALSGLTSINMHDLESGRLDDDQWENLAKCFDDISRKKLFFYDKSYLRINQVRLQLRKLKSQHKELGIAFIDYLQLMSG
SKANTERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEAQELRLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEEGQETK
FEIPIT
>Mature_486_residues
MNSAEQLQRLLEIERIVLSGIVFANHKIEEIHSVLEPSDFYYPPHRLFFEIAFKLHEENYPVDENFIRQKMPKDKQINEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLLALANTLREQSLENSQKSSDILNAIEREVYTLLNGSTIEGFRDIQEVLAS
TMNIITENQKKGSLEVTGIPTGFAQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDKGVAVFSLEMSAEQLA
LRALSGLTSINMHDLESGRLDDDQWENLAKCFDDISRKKLFFYDKSYLRINQVRLQLRKLKSQHKELGIAFIDYLQLMSG
SKANTERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEAQELRLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEEGQETK
FEIPIT

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=466, Percent_Identity=32.8326180257511, Blast_Score=262, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55623; Mature: 55623

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAEQLQRLLEIERIVLSGIVFANHKIEEIHSVLEPSDFYYPPHRLFFEIAFKLHEENY
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
PVDENFIRQKMPKDKQINEEDLVAIFAASPIDNIEAYVEEIKNASIKRKLLALANTLREQ
CCCHHHHHHHCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
SLENSQKSSDILNAIEREVYTLLNGSTIEGFRDIQEVLASTMNIITENQKKGSLEVTGIP
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGFAQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDKGVAVFSLEMSAEQLA
CCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
LRALSGLTSINMHDLESGRLDDDQWENLAKCFDDISRKKLFFYDKSYLRINQVRLQLRKL
HHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHH
KSQHKELGIAFIDYLQLMSGSKANTERHEQIAEISRELKTLARELEIPIIALVQLNRSLE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEAQELRL
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCHHEEECCCCCHHHHHHHCCCHHHHHHHHH
KVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEEGQETK
HCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCCC
FEIPIT
EECCCC
>Mature Secondary Structure
MNSAEQLQRLLEIERIVLSGIVFANHKIEEIHSVLEPSDFYYPPHRLFFEIAFKLHEENY
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
PVDENFIRQKMPKDKQINEEDLVAIFAASPIDNIEAYVEEIKNASIKRKLLALANTLREQ
CCCHHHHHHHCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
SLENSQKSSDILNAIEREVYTLLNGSTIEGFRDIQEVLASTMNIITENQKKGSLEVTGIP
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGFAQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDKGVAVFSLEMSAEQLA
CCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
LRALSGLTSINMHDLESGRLDDDQWENLAKCFDDISRKKLFFYDKSYLRINQVRLQLRKL
HHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHH
KSQHKELGIAFIDYLQLMSGSKANTERHEQIAEISRELKTLARELEIPIIALVQLNRSLE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEAQELRL
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCHHEEECCCCCHHHHHHHCCCHHHHHHHHH
KVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEEGQETK
HCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCCC
FEIPIT
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]