Click here to switch to the map view.

The map label for this gene is ksgA

Identifier: 109946738

GI number: 109946738

Start: 102975

End: 103790

Strand: Direct

Name: ksgA

Synonym: Hac_0113

Alternate gene names: 109946738

Gene position: 102975-103790 (Clockwise)

Preceding gene: 109946736

Following gene: 109946739

Centisome position: 6.63

GC content: 36.64

Gene sequence:

>816_bases
ATGGTAGTAGCTAAAAAGTCTTTAGGGCAGCATTTTTTAACGGACGAGTCGTTTTTAGATCGTATTGTTAGTGCTTTGCC
CCCCTTAAATTCGTTAAGATTGATTGAAATTGGCGTGGGGCTAGGGGATTTGACTCTTAAATTGTTAGATCGCTATCCTT
TAAAGACTTATGAGATAGATAGCAGTTTGTGCGAGAAAATGCGAGAAAGGCTAAAAAAGCAAAAAAAACCTTTTGAGTTG
GAATTAGCAGAAAAAGACGCTCTTTTTTTAAAAGAAGAAGAGCCTTATTTTTTGATCTCTAATTTGCCTTATTATATCGC
TACTAGGCTTGTTTTAAATGCGTTTAAAGACCCTAAATGCAGGGGTTTATTGGTGATGACACAAAAGGAAGTGGCGTTAA
AATTTTGCACTAAAGATTCACAGAATGCTTTAAGCGTTTTGGCTCACGCCATAGGGAATGTTACCCTTTTATTTGATGTG
CCACCTAGCGCGTTTAGCCCATCTCCAAAGGTGTTTTCTAGCATGTTTGAAGTGATCAAAGAGCCGTTGAAAGAAAAGGC
GTTAGCGTCATTAATTCCAACGCTATCTTTTGAAGAAGCCCTACAAAAAGGGTTTGAAACATTAGAAGATTTTTTGAAAG
CTTGTTTCTCATCTCCTAGGAAGACGCTTTCAAACAATCTTAAAAAAAGCGTTTCTTATAAAGAAAAGCTTGATAAGGTG
TTAGATTTTTTAGCGTTAGAAAACCAACCAACAAGCGTGAGAGCGTCTGAGATACAAGATTATCTCAAGCTCTTAAAATA
CCTTTTAAAAGGCTAA

Upstream 100 bases:

>100_bases
AGCGATCTAGCTTTTAGGGTTATCAAAACTTTAAATCAAAAAAGCTATAATGGAAGAGTTTTAAATTTTAGGGTATAATA
TTAGGAATTAAAAGCGTGCT

Downstream 100 bases:

>100_bases
TACCTTTTAAAAAATTGAAATGAAGGAAAGAAAGTCATGAGTGATAATGACCATTTTGAAAACAACAGACACAATGAACA
TAATGAACATAGTGAAAACT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVVAKKSLGQHFLTDESFLDRIVSALPPLNSLRLIEIGVGLGDLTLKLLDRYPLKTYEIDSSLCEKMRERLKKQKKPFEL
ELAEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKCRGLLVMTQKEVALKFCTKDSQNALSVLAHAIGNVTLLFDV
PPSAFSPSPKVFSSMFEVIKEPLKEKALASLIPTLSFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYKEKLDKV
LDFLALENQPTSVRASEIQDYLKLLKYLLKG

Sequences:

>Translated_271_residues
MVVAKKSLGQHFLTDESFLDRIVSALPPLNSLRLIEIGVGLGDLTLKLLDRYPLKTYEIDSSLCEKMRERLKKQKKPFEL
ELAEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKCRGLLVMTQKEVALKFCTKDSQNALSVLAHAIGNVTLLFDV
PPSAFSPSPKVFSSMFEVIKEPLKEKALASLIPTLSFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYKEKLDKV
LDFLALENQPTSVRASEIQDYLKLLKYLLKG
>Mature_271_residues
MVVAKKSLGQHFLTDESFLDRIVSALPPLNSLRLIEIGVGLGDLTLKLLDRYPLKTYEIDSSLCEKMRERLKKQKKPFEL
ELAEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKCRGLLVMTQKEVALKFCTKDSQNALSVLAHAIGNVTLLFDV
PPSAFSPSPKVFSSMFEVIKEPLKEKALASLIPTLSFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYKEKLDKV
LDFLALENQPTSVRASEIQDYLKLLKYLLKG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=292, Percent_Identity=24.6575342465753, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786236, Length=283, Percent_Identity=29.3286219081272, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25146882, Length=288, Percent_Identity=26.3888888888889, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324989, Length=265, Percent_Identity=27.5471698113208, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357273, Length=205, Percent_Identity=29.7560975609756, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21358017, Length=181, Percent_Identity=29.2817679558011, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_HELAH (Q17ZF9)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_663966.1
- ProteinModelPortal:   Q17ZF9
- SMR:   Q17ZF9
- STRING:   Q17ZF9
- GeneID:   4176138
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0113
- NMPDR:   fig|382638.8.peg.110
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   YYIATRL
- ProtClustDB:   PRK00274
- BioCyc:   HACI382638:HAC_0113-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30702; Mature: 30702

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 11-11 BINDING 13-13 BINDING 38-38 BINDING 58-58 BINDING 86-86 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVAKKSLGQHFLTDESFLDRIVSALPPLNSLRLIEIGVGLGDLTLKLLDRYPLKTYEID
CCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEECC
SSLCEKMRERLKKQKKPFELELAEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKC
HHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHCCCCCC
RGLLVMTQKEVALKFCTKDSQNALSVLAHAIGNVTLLFDVPPSAFSPSPKVFSSMFEVIK
CEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EPLKEKALASLIPTLSFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYKEKLDKV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LDFLALENQPTSVRASEIQDYLKLLKYLLKG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVAKKSLGQHFLTDESFLDRIVSALPPLNSLRLIEIGVGLGDLTLKLLDRYPLKTYEID
CCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEECC
SSLCEKMRERLKKQKKPFELELAEKDALFLKEEEPYFLISNLPYYIATRLVLNAFKDPKC
HHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHCCCCCC
RGLLVMTQKEVALKFCTKDSQNALSVLAHAIGNVTLLFDVPPSAFSPSPKVFSSMFEVIK
CEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EPLKEKALASLIPTLSFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYKEKLDKV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LDFLALENQPTSVRASEIQDYLKLLKYLLKG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA