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The map label for this gene is insB [H]

Identifier: 108813493

GI number: 108813493

Start: 3800781

End: 3801083

Strand: Reverse

Name: insB [H]

Synonym: YPN_3333

Alternate gene names: 108813493

Gene position: 3801083-3800781 (Counterclockwise)

Preceding gene: 108813494

Following gene: 108813491

Centisome position: 83.82

GC content: 43.23

Gene sequence:

>303_bases
GTGCTGCTTACGCCCTTCAATATCGGTATGATCACCCGCGATGACTGGGGTAGCTATGCTAGGCTCGTACCGCAAGATAG
CCATCTGACTGGCAAAATCTTTACTCAGCGCATTGAACGCAACAACCTGACTTTACGTACATACATCAAAAGGCTCGCGC
GCAAAACTATCTGCTTCTCACGCTCTACTGAGCTACATGAAAAAGTTATCGAAACCTGCATTGAAAAATACATGTTCTAC
TCATTGGAGGTATCACCCAAAAAATCAGACAAATTGAATCTTTTTAGTCGAATATGGCTTTAG

Upstream 100 bases:

>100_bases
AGTAACTTAGGTATAAGCCTATTAAGTTAGAGGTGATTTAATTTATGTACTGGCCTATACTTTCGGACCGTGCACGAATG
AAACTTGTCGTGAATTACTG

Downstream 100 bases:

>100_bases
AAATAACAAAACCCGCACATTGGCGGGTTCGTTGACATTAAAAGGAAACATTCACTTTAATGTCAATGCACGGTAAATTT
ATGTTTTTTTCTATCCGGGC

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 100; Mature: 100

Protein sequence:

>100_residues
MLLTPFNIGMITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFSRSTELHEKVIETCIEKYMFY
SLEVSPKKSDKLNLFSRIWL

Sequences:

>Translated_100_residues
MLLTPFNIGMITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFSRSTELHEKVIETCIEKYMFY
SLEVSPKKSDKLNLFSRIWL
>Mature_100_residues
MLLTPFNIGMITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFSRSTELHEKVIETCIEKYMFY
SLEVSPKKSDKLNLFSRIWL

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=69, Percent_Identity=46.3768115942029, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI1788203, Length=69, Percent_Identity=46.3768115942029, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI1786203, Length=69, Percent_Identity=46.3768115942029, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI1787223, Length=69, Percent_Identity=46.3768115942029, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1786470, Length=69, Percent_Identity=44.9275362318841, Blast_Score=65, Evalue=6e-13,
Organism=Escherichia coli, GI1786459, Length=69, Percent_Identity=44.9275362318841, Blast_Score=65, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 11917; Mature: 11917

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTPFNIGMITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFS
CEECCCCCCEEECCCCCCCEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
RSTELHEKVIETCIEKYMFYSLEVSPKKSDKLNLFSRIWL
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MLLTPFNIGMITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFS
CEECCCCCCEEECCCCCCCEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
RSTELHEKVIETCIEKYMFYSLEVSPKKSDKLNLFSRIWL
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]