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The map label for this gene is outE [H]

Identifier: 108813179

GI number: 108813179

Start: 3390038

End: 3391399

Strand: Reverse

Name: outE [H]

Synonym: YPN_3019

Alternate gene names: 108813179

Gene position: 3391399-3390038 (Counterclockwise)

Preceding gene: 108813180

Following gene: 108813178

Centisome position: 74.79

GC content: 38.99

Gene sequence:

>1362_bases
GTGGCTCATAGTCCAATACTTTTTAATTTAGTTACTGATGAAGAGTTTGAAAATAATTTAACTGAACGCTACCAAAATAA
TTCGGCTGACCCATATCAGACAATGAGCGTTATCAGCAATGAGATCGACATTTACTCTTTTGCTAACGAATTATCTGACG
ATGATGATTTATTGGATATAAATGATGAAGCACCTGTAATAAAATTAATCAATAGCATTCTGGTCGAAGCAATTAAAGAG
TTAGCATCTGATATACACATAGAGTCTTTCGATAAAAAACTTACTGTAAGATTTCGTATTGATGGTGTGTTAAGGAAAAT
ATTGGAATTACAGCGCCATGTTGCACTATTATTAGTGTCGCGTATAAAAGTCATGGCCAAATTAGATATTGCAGAAAAAC
GTATTCCACAAGATGGGCGCATAGCTCTAAATTTAGCGGGTAGAGCATTGGATGTTCGTGTTTCGGTATTACCATCTAGC
CATGGTGAACGGGTCGTTATGCGTTTGCTTGACAAAAATAGCATTAAATTGGATTTACCCTCATTAGGTATGTCGAAAGA
TAACTGTATTTCTATGAATACGCAGGTACATAAACCCCATGGCATTATCTTGGTTGTTGGCCCAACGGGTTCGGGTAAAA
GCACTACGTTATATGCTGCTTTAATGGGGATTGACGCGAATGAGAAAAATATTATGACTGTAGAAGACCCTATTGAGTTC
GACATACCTGGTATATCCCAAACGCAAGTAAACCCAAAAATTGAAATGACATTTGCTCGTAGCTTAAGGGCAATATTACG
CCAAGATCCTGACGTTATTCTTATCGGTGAAATTCGTGATATTGAAACGGCACAGATAGCGGTCCAAGCTTCTCTAACCG
GCCATTTAGTTCTATCTACATTACATGCCAACAGTGCGATAGGTGCAGTGACCAGACTGAAGGATATGAATGTAGAGGCA
TTTATGCTTTCAAGTTCATTATTGGCCATCATATCTCAGCGGTTGGTTAGAAGATTATGTATAGCGTGTCGCCAAGAGTC
GTCAGTGACGGAGAAGGTATTACAACGTCTGAATATTGATGATAAATGCTCTTTGGTTGGTTCGGTGTACCGGGCTAAAG
GTTGTAACAAATGTAATCTTACTGGTTATCGTGGACGTATTGCTCTCCATGAATTTCTCGTAGTGGATAACTTATTGCGG
GGGGCTATTTATAAAGGGCTAGGAGAGTTTGAACTGGCTAAATTGGCCAGTGGGGCTATAAACAGCCTTCTATCTGATGG
GATAAGCAAAGTTATTGCAGGGTTAACCACTATAGAGGAACTGGTAAGAATATGCCAAGAGGATGATAATGGCTGTATTT
AA

Upstream 100 bases:

>100_bases
GTCAAAGAGTCATGGTGTGCTTATTCTGCCTATGGCACAGGGCAGTCAGTTGATTTGTCGTAAATCAGCGACATTGGAGG
CTATACTTGAAGGGAATCGG

Downstream 100 bases:

>100_bases
ATATGTTGCCATCAGCAGAAGTGGAACCAAGATAACAGGTGATATTGATGCGGAAAACATACGTATAGCCCGTTATCTCC
TTTACAAAAAAAACATGCAT

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MAHSPILFNLVTDEEFENNLTERYQNNSADPYQTMSVISNEIDIYSFANELSDDDDLLDINDEAPVIKLINSILVEAIKE
LASDIHIESFDKKLTVRFRIDGVLRKILELQRHVALLLVSRIKVMAKLDIAEKRIPQDGRIALNLAGRALDVRVSVLPSS
HGERVVMRLLDKNSIKLDLPSLGMSKDNCISMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEF
DIPGISQTQVNPKIEMTFARSLRAILRQDPDVILIGEIRDIETAQIAVQASLTGHLVLSTLHANSAIGAVTRLKDMNVEA
FMLSSSLLAIISQRLVRRLCIACRQESSVTEKVLQRLNIDDKCSLVGSVYRAKGCNKCNLTGYRGRIALHEFLVVDNLLR
GAIYKGLGEFELAKLASGAINSLLSDGISKVIAGLTTIEELVRICQEDDNGCI

Sequences:

>Translated_453_residues
MAHSPILFNLVTDEEFENNLTERYQNNSADPYQTMSVISNEIDIYSFANELSDDDDLLDINDEAPVIKLINSILVEAIKE
LASDIHIESFDKKLTVRFRIDGVLRKILELQRHVALLLVSRIKVMAKLDIAEKRIPQDGRIALNLAGRALDVRVSVLPSS
HGERVVMRLLDKNSIKLDLPSLGMSKDNCISMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEF
DIPGISQTQVNPKIEMTFARSLRAILRQDPDVILIGEIRDIETAQIAVQASLTGHLVLSTLHANSAIGAVTRLKDMNVEA
FMLSSSLLAIISQRLVRRLCIACRQESSVTEKVLQRLNIDDKCSLVGSVYRAKGCNKCNLTGYRGRIALHEFLVVDNLLR
GAIYKGLGEFELAKLASGAINSLLSDGISKVIAGLTTIEELVRICQEDDNGCI
>Mature_452_residues
AHSPILFNLVTDEEFENNLTERYQNNSADPYQTMSVISNEIDIYSFANELSDDDDLLDINDEAPVIKLINSILVEAIKEL
ASDIHIESFDKKLTVRFRIDGVLRKILELQRHVALLLVSRIKVMAKLDIAEKRIPQDGRIALNLAGRALDVRVSVLPSSH
GERVVMRLLDKNSIKLDLPSLGMSKDNCISMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEFD
IPGISQTQVNPKIEMTFARSLRAILRQDPDVILIGEIRDIETAQIAVQASLTGHLVLSTLHANSAIGAVTRLKDMNVEAF
MLSSSLLAIISQRLVRRLCIACRQESSVTEKVLQRLNIDDKCSLVGSVYRAKGCNKCNLTGYRGRIALHEFLVVDNLLRG
AIYKGLGEFELAKLASGAINSLLSDGISKVIAGLTTIEELVRICQEDDNGCI

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=435, Percent_Identity=54.0229885057471, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=43.455497382199, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI87082188, Length=207, Percent_Identity=40.0966183574879, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 49936; Mature: 49804

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHSPILFNLVTDEEFENNLTERYQNNSADPYQTMSVISNEIDIYSFANELSDDDDLLDI
CCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEC
NDEAPVIKLINSILVEAIKELASDIHIESFDKKLTVRFRIDGVLRKILELQRHVALLLVS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
RIKVMAKLDIAEKRIPQDGRIALNLAGRALDVRVSVLPSSHGERVVMRLLDKNSIKLDLP
HHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEECC
SLGMSKDNCISMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEF
CCCCCCCCEEECCCEEECCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEECCCCEE
DIPGISQTQVNPKIEMTFARSLRAILRQDPDVILIGEIRDIETAQIAVQASLTGHLVLST
CCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEE
LHANSAIGAVTRLKDMNVEAFMLSSSLLAIISQRLVRRLCIACRQESSVTEKVLQRLNID
HHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
DKCSLVGSVYRAKGCNKCNLTGYRGRIALHEFLVVDNLLRGAIYKGLGEFELAKLASGAI
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NSLLSDGISKVIAGLTTIEELVRICQEDDNGCI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AHSPILFNLVTDEEFENNLTERYQNNSADPYQTMSVISNEIDIYSFANELSDDDDLLDI
CCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEC
NDEAPVIKLINSILVEAIKELASDIHIESFDKKLTVRFRIDGVLRKILELQRHVALLLVS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
RIKVMAKLDIAEKRIPQDGRIALNLAGRALDVRVSVLPSSHGERVVMRLLDKNSIKLDLP
HHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEECC
SLGMSKDNCISMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEF
CCCCCCCCEEECCCEEECCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEECCCCEE
DIPGISQTQVNPKIEMTFARSLRAILRQDPDVILIGEIRDIETAQIAVQASLTGHLVLST
CCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEE
LHANSAIGAVTRLKDMNVEAFMLSSSLLAIISQRLVRRLCIACRQESSVTEKVLQRLNID
HHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
DKCSLVGSVYRAKGCNKCNLTGYRGRIALHEFLVVDNLLRGAIYKGLGEFELAKLASGAI
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NSLLSDGISKVIAGLTTIEELVRICQEDDNGCI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]