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The map label for this gene is pksI [H]

Identifier: 108812680

GI number: 108812680

Start: 2830836

End: 2831612

Strand: Reverse

Name: pksI [H]

Synonym: YPN_2519

Alternate gene names: 108812680

Gene position: 2831612-2830836 (Counterclockwise)

Preceding gene: 108812681

Following gene: 108812679

Centisome position: 62.44

GC content: 39.51

Gene sequence:

>777_bases
ATGATGAATATGCGGATGACTAATATGAAAAAAATTTATTTAAGAAAAAGTGATGAAGGTATTGCTTTTATGCATATTAA
CGATCCAGTCAACAAAAATGCCATTGATGATGAGTTTTGTTTACAGTTACATGGTATTGTCGACACGATAAAGCAAGACA
AAACCATCAAAGTGCTTATTTTGGTTGGATTGCCTGATATATTTTGTACCGGTGGAACCGAAGCGTTTTTACAAAGCCTA
ATCACACACTACAAAACTAATATTGAACACGAGTATGCCAATCACCTCAAACATTTTCTGAATATACCGATCCCGGTTAT
TGCGGCGATGGAAGGCAGTGCTACAGGGGGGGGATTAGTCTTAGGTTTATATGCAGATATTTTAGTCGCGGCGGAAGAAA
GTCGCTATGGCATGTCATTTATGAACATGGGTTTCACCCCTGGGATGGGTTCAACTGCCATGAGTAAGGAGGCGTTTGGT
TATTACACCGGTTTTGAGATGATGGTCACCGGTAACTATATTAGGGGCAGTGAGCTGAAAGGAAAATGTTGTTTCAACTA
TATTTTGCCCCGCGCCATGGTCATGGAAAAGGCCATTGAACTGGCAACCTCTATTGCTGAAAAACCTCGCCATAGTTTGG
AATTATTAAAAAAATACATGTCAATCGACCGGCGTAAAATGCTAGAGGAAACAAGCACTATCGAGGCGTTTATGCACCAT
ATTGCATTTAATCAAAGCAATATCGAACAACAAATACAAGATAATTACACGGGTTAG

Upstream 100 bases:

>100_bases
GAAAGTTTGGATTTAGGTAGCCATCTTCTGGTGGAGAGACTAAATGATGCTCAGACCATAGAAAAGATACGTCACTACTT
AGAAGACATTAAGTTATTTA

Downstream 100 bases:

>100_bases
ATATGCTGAATAACTTGAGTGAAATGACCGTATTGGTAACTGGTGGCACAAAAGGTATAGGCCGAGCCACTGTTGAAAGC
TTTGTGAAGGCGGGAGCAAA

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MMNMRMTNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLILVGLPDIFCTGGTEAFLQSL
ITHYKTNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLVLGLYADILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFG
YYTGFEMMVTGNYIRGSELKGKCCFNYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHH
IAFNQSNIEQQIQDNYTG

Sequences:

>Translated_258_residues
MMNMRMTNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLILVGLPDIFCTGGTEAFLQSL
ITHYKTNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLVLGLYADILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFG
YYTGFEMMVTGNYIRGSELKGKCCFNYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHH
IAFNQSNIEQQIQDNYTG
>Mature_258_residues
MMNMRMTNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLILVGLPDIFCTGGTEAFLQSL
ITHYKTNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLVLGLYADILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFG
YYTGFEMMVTGNYIRGSELKGKCCFNYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHH
IAFNQSNIEQQIQDNYTG

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3-methylglutaryl group attached to pksL [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=229, Percent_Identity=25.764192139738, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4502327, Length=178, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=194, Percent_Identity=26.2886597938144, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=215, Percent_Identity=28.3720930232558, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=22.5806451612903, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=240, Percent_Identity=25, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=27.8606965174129, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=203, Percent_Identity=25.615763546798, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=202, Percent_Identity=25.7425742574257, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653139, Length=214, Percent_Identity=26.1682242990654, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=24.2798353909465, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=24.2798353909465, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24650670, Length=239, Percent_Identity=25.1046025104602, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 29080; Mature: 29080

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
7.4 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
7.4 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNMRMTNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLI
CCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
LVGLPDIFCTGGTEAFLQSLITHYKTNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLV
EECCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHEEEECCCCCCCHHH
LGLYADILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFGYYTGFEMMVTGNYIRGSELK
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCEECCEEEEEECCEEECCCCC
GKCCFNYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHH
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFNQSNIEQQIQDNYTG
HHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MMNMRMTNMKKIYLRKSDEGIAFMHINDPVNKNAIDDEFCLQLHGIVDTIKQDKTIKVLI
CCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
LVGLPDIFCTGGTEAFLQSLITHYKTNIEHEYANHLKHFLNIPIPVIAAMEGSATGGGLV
EECCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHEEEECCCCCCCHHH
LGLYADILVAAEESRYGMSFMNMGFTPGMGSTAMSKEAFGYYTGFEMMVTGNYIRGSELK
HHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCEECCEEEEEECCEEECCCCC
GKCCFNYILPRAMVMEKAIELATSIAEKPRHSLELLKKYMSIDRRKMLEETSTIEAFMHH
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFNQSNIEQQIQDNYTG
HHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]