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The map label for this gene is pncA [H]

Identifier: 108811789

GI number: 108811789

Start: 1837996

End: 1838643

Strand: Reverse

Name: pncA [H]

Synonym: YPN_1626

Alternate gene names: 108811789

Gene position: 1838643-1837996 (Counterclockwise)

Preceding gene: 108811790

Following gene: 108811786

Centisome position: 40.55

GC content: 49.69

Gene sequence:

>648_bases
ATGAAAGCAGCCTTGCTCTTAATCGATTTACAAAACGACTTTTGTCCTGGTGGAGCGTTGGCTGTTAGTGAAGGTGATGA
GGTCATTAGCGTGGCTAACCGCATGATTGAAGCCTGTCTCGCAAAAAACATACCGGTGATTGCCAGCCAAGATTGGCACC
CAATTGAGCACCGTAGCTTTGCGGTGAACGCCAAGGCGGAGCCGGGCACAGTTGGGGAACTAGAAGGGTTAGTACAAGTA
TGGTGGCCGGTACATTGTGTTCAGGAACAACCAGGGGCAGCATTGCACCCACAGCTGAAACAAGGCGCTATTACAGCTAT
TTTTCGCAAAGGGCAAAACCCTGATATCGACAGTTACAGCACTTTCTTCGACAATGGCCGGCGAGCTAAAACAGCATTAG
ATAGCTGGCTACAACAGCATAGCATTGACCACCTGTATGTTATGGGGCTGGCCACAGATTACTGTGTGAAATATAGCGTA
CTGGATGCACTCGCTTTGGGTTATCAAACCACGGTTATCAGTGATGGGTGTCGTGGGGTAAACTTGCAACCACAAGACAG
CCAACTGGCTTTCGATGCAATGAGCGATGCAGGCGCCAACTTACTGGCTCTTGAACAATGCCTGGCTGAAGTCGTTTCAG
CCGACTGA

Upstream 100 bases:

>100_bases
ATTTGTTGAGCCAATCTTTATCTCCTAACGAGATTCGCCAACTGATGCAGCAAAATTTACGTGGTGAATTAACCGATACT
CAATAACTTTTGGGGTTTTA

Downstream 100 bases:

>100_bases
TCGCTTTCTGCGCAAAAATGGGCGCCTGCCTCGGCGCCCATTTTTTGTATTCAGAAAACCCTCTGCCAGGCAGCAATGCC
GGAGGATATTGATTATGTGA

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKAALLLIDLQNDFCPGGALAVSEGDEVISVANRMIEACLAKNIPVIASQDWHPIEHRSFAVNAKAEPGTVGELEGLVQV
WWPVHCVQEQPGAALHPQLKQGAITAIFRKGQNPDIDSYSTFFDNGRRAKTALDSWLQQHSIDHLYVMGLATDYCVKYSV
LDALALGYQTTVISDGCRGVNLQPQDSQLAFDAMSDAGANLLALEQCLAEVVSAD

Sequences:

>Translated_215_residues
MKAALLLIDLQNDFCPGGALAVSEGDEVISVANRMIEACLAKNIPVIASQDWHPIEHRSFAVNAKAEPGTVGELEGLVQV
WWPVHCVQEQPGAALHPQLKQGAITAIFRKGQNPDIDSYSTFFDNGRRAKTALDSWLQQHSIDHLYVMGLATDYCVKYSV
LDALALGYQTTVISDGCRGVNLQPQDSQLAFDAMSDAGANLLALEQCLAEVVSAD
>Mature_215_residues
MKAALLLIDLQNDFCPGGALAVSEGDEVISVANRMIEACLAKNIPVIASQDWHPIEHRSFAVNAKAEPGTVGELEGLVQV
WWPVHCVQEQPGAALHPQLKQGAITAIFRKGQNPDIDSYSTFFDNGRRAKTALDSWLQQHSIDHLYVMGLATDYCVKYSV
LDALALGYQTTVISDGCRGVNLQPQDSQLAFDAMSDAGANLLALEQCLAEVVSAD

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=201, Percent_Identity=65.1741293532338, Blast_Score=272, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI193206735, Length=215, Percent_Identity=31.6279069767442, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17543130, Length=215, Percent_Identity=31.6279069767442, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71999339, Length=202, Percent_Identity=27.7227722772277, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321401, Length=198, Percent_Identity=33.8383838383838, Blast_Score=118, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648247, Length=203, Percent_Identity=34.9753694581281, Blast_Score=118, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23252; Mature: 23252

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAALLLIDLQNDFCPGGALAVSEGDEVISVANRMIEACLAKNIPVIASQDWHPIEHRSF
CCEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEE
AVNAKAEPGTVGELEGLVQVWWPVHCVQEQPGAALHPQLKQGAITAIFRKGQNPDIDSYS
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHH
TFFDNGRRAKTALDSWLQQHSIDHLYVMGLATDYCVKYSVLDALALGYQTTVISDGCRGV
HHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCHHHEEECCCCCCC
NLQPQDSQLAFDAMSDAGANLLALEQCLAEVVSAD
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAALLLIDLQNDFCPGGALAVSEGDEVISVANRMIEACLAKNIPVIASQDWHPIEHRSF
CCEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEE
AVNAKAEPGTVGELEGLVQVWWPVHCVQEQPGAALHPQLKQGAITAIFRKGQNPDIDSYS
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHH
TFFDNGRRAKTALDSWLQQHSIDHLYVMGLATDYCVKYSVLDALALGYQTTVISDGCRGV
HHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCHHHEEECCCCCCC
NLQPQDSQLAFDAMSDAGANLLALEQCLAEVVSAD
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]