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The map label for this gene is fadD21 [H]

Identifier: 108801974

GI number: 108801974

Start: 5309897

End: 5311786

Strand: Reverse

Name: fadD21 [H]

Synonym: Mmcs_5011

Alternate gene names: 108801974

Gene position: 5311786-5309897 (Counterclockwise)

Preceding gene: 108801975

Following gene: 108801973

Centisome position: 93.1

GC content: 68.57

Gene sequence:

>1890_bases
ATGCCGTTCCACAACCCGTTCATCAAGGACGGCCTCATCAAGTTCCCCGACAACGGGAACCTGGTGCGTCACGTCGAGCG
ATGGGCGAAGGTGCGTGGCGACAAACTGGCGTACCGCTTCATCGACTTCTCCACCGAACGCGACGGGGTCGAGCGCGACC
TCACCTGGGCCGACTTCGGCGGCCGCAACCGCGCCGTCGGCGCCCGCCTGCAGCAGGTGACCCAGCCCGGCGACCGCGTC
GCGATCCTGTGCCCGCAGAACCTCGACTACCTCGTCGCATTCTTCGGCACGCTGTACTCCGGCCGCATCGCCGTGCCGCT
GTTCGACCCGAACGAGCCCGGCCACGTCGGACGCCTGCACGCCGTCCTCGACGACTGCACACCGTCGGCGATCCTGACCA
CCACCGAGGCCGCCGAGGGTGTGCGCAAGTTCTTCCGCACCCGGCCGGCCAACCAGCGTCCCCGCGTCATCGCCGTCGAC
GCGGTCCCCAACGAGGTCGGTCAGACCTGGGAGCCCGTCGACGTCCACCAGGAGACCATCGCGTACCTGCAGTACACCTC
CGGTTCCACGCGTATCCCGACCGGTGTGCAGATCACCCACCTGAACCTGGCCACCAACGTGGTGCAGGTGATCGAGGCGC
TCGAGGGTGAGGAAGGCGACCGGGGTGTGTCCTGGCTGCCGTTCTTCCACGACATGGGTCTGATCACGATCATGATCTCG
CCGATGATCGGGCACTACATCTCGTTCATGACGCCCGCCGCGTTCGTCCGCCGCCCCGGCCGCTGGATCCGCGAGATGGC
CCGCAAGGAGGGCGAGACCGGCGGCGTCATCTCCGTCGCGCCGAACTTCGCGTTCGACCACGCCGCCGCCCGCGGAGTGC
CCCGCGAGGGCGAGCCGGCGCTGGACCTGTCCAACGTCAAGGCGCTCCTCAACGGCAGTGAACCGATCTCGGCGGCGACG
GTCCGCAACTTCAACGAGGCGTTCGGGCCGTTCGGGTTCAAACCGCAGGCCATCAAACCGTCCTACGGGCTGGCCGAGGC
CACGCTGTTCGTGTCGACGACCCCGGTCAACGAATCGCCGAAGATCGTCACCGTCGACCGCGACGAACTGAACAGCCACC
GCTTCGTCGAGGTGCCGCCGGATTCGCCGAAGGCCGTCGCGCAGGCGTCGGCGGGCAAGGTCGGCGTCGCGGAATGGGCG
GTCATCGTCGACCACGACACCGCCACCGAACTGCCCGACGGCCAGATCGGCGAGATCTGGATCTCCGGGCAGAACATGGG
CACCGGCTACTGGGGCAAGGAAGAGGAGACCCAGGCGACATTCCGCAACATCCTCAAGTCGCGGACGAACCCGAGCCACG
CCGAAGGCGCGCCCGACGACGCGATGTGGGTGCGCACCGGTGACCTCGGCGCCTTCCACGACGGCGACCTCTACATCACC
GGCCGCGTCAAGGACCTGGTGATCATCGACGGCCGCAACCACTATCCGCAGGATCTCGAGTACTCGGCGCAGGAGGCCAC
CAAGGCGCTGCGCACGGGATTCGTCGCGGCGTTCTCGGTGCCGGCCAACCAGCTTCCCGACGAGGTCTTCGACAACGCGC
ACGCCGGTCTCAAGCGCGACCCCTCCGACACCTCCGAACAGCTCGTCATCGTCGGTGAGCGGGCGCCCGGTGCCCACAAG
CTCGACATGGGCCCGATCGCCGACGACATCCGCGCCGCGATCGCCGTGCGCCACGGCGTCACGGTGCGCGACGTGCTGCT
GACCCCGGCCGGAGCGATCCCGCGTACGTCGAGCGGCAAGATCGGCCGCCGGGCGTGCCGCTCGGCCTACCTCGACGGAA
GCCTCCGCAGCGGGAAGGTCGCCAACGCCTTCCCCGACGAGACGGACTGA

Upstream 100 bases:

>100_bases
GAGTAACATTTTGAGGTTCGGGTCCGCGCCGTCCGCCGAGAGACTGCAGCGCCCGTGAGAAAGCCGTGCAGAAAGCCGCG
GTTTGACAGGAGAGTGTGAA

Downstream 100 bases:

>100_bases
CGAGACGGACTGACGCCCCCTCACACATACACCGCCGCGGGCGGCGAAAGAGATGAACATGTCTGAAACACCGAACAATT
CGCCCTCCGCTGAGAATCAG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MPFHNPFIKDGLIKFPDNGNLVRHVERWAKVRGDKLAYRFIDFSTERDGVERDLTWADFGGRNRAVGARLQQVTQPGDRV
AILCPQNLDYLVAFFGTLYSGRIAVPLFDPNEPGHVGRLHAVLDDCTPSAILTTTEAAEGVRKFFRTRPANQRPRVIAVD
AVPNEVGQTWEPVDVHQETIAYLQYTSGSTRIPTGVQITHLNLATNVVQVIEALEGEEGDRGVSWLPFFHDMGLITIMIS
PMIGHYISFMTPAAFVRRPGRWIREMARKEGETGGVISVAPNFAFDHAAARGVPREGEPALDLSNVKALLNGSEPISAAT
VRNFNEAFGPFGFKPQAIKPSYGLAEATLFVSTTPVNESPKIVTVDRDELNSHRFVEVPPDSPKAVAQASAGKVGVAEWA
VIVDHDTATELPDGQIGEIWISGQNMGTGYWGKEEETQATFRNILKSRTNPSHAEGAPDDAMWVRTGDLGAFHDGDLYIT
GRVKDLVIIDGRNHYPQDLEYSAQEATKALRTGFVAAFSVPANQLPDEVFDNAHAGLKRDPSDTSEQLVIVGERAPGAHK
LDMGPIADDIRAAIAVRHGVTVRDVLLTPAGAIPRTSSGKIGRRACRSAYLDGSLRSGKVANAFPDETD

Sequences:

>Translated_629_residues
MPFHNPFIKDGLIKFPDNGNLVRHVERWAKVRGDKLAYRFIDFSTERDGVERDLTWADFGGRNRAVGARLQQVTQPGDRV
AILCPQNLDYLVAFFGTLYSGRIAVPLFDPNEPGHVGRLHAVLDDCTPSAILTTTEAAEGVRKFFRTRPANQRPRVIAVD
AVPNEVGQTWEPVDVHQETIAYLQYTSGSTRIPTGVQITHLNLATNVVQVIEALEGEEGDRGVSWLPFFHDMGLITIMIS
PMIGHYISFMTPAAFVRRPGRWIREMARKEGETGGVISVAPNFAFDHAAARGVPREGEPALDLSNVKALLNGSEPISAAT
VRNFNEAFGPFGFKPQAIKPSYGLAEATLFVSTTPVNESPKIVTVDRDELNSHRFVEVPPDSPKAVAQASAGKVGVAEWA
VIVDHDTATELPDGQIGEIWISGQNMGTGYWGKEEETQATFRNILKSRTNPSHAEGAPDDAMWVRTGDLGAFHDGDLYIT
GRVKDLVIIDGRNHYPQDLEYSAQEATKALRTGFVAAFSVPANQLPDEVFDNAHAGLKRDPSDTSEQLVIVGERAPGAHK
LDMGPIADDIRAAIAVRHGVTVRDVLLTPAGAIPRTSSGKIGRRACRSAYLDGSLRSGKVANAFPDETD
>Mature_628_residues
PFHNPFIKDGLIKFPDNGNLVRHVERWAKVRGDKLAYRFIDFSTERDGVERDLTWADFGGRNRAVGARLQQVTQPGDRVA
ILCPQNLDYLVAFFGTLYSGRIAVPLFDPNEPGHVGRLHAVLDDCTPSAILTTTEAAEGVRKFFRTRPANQRPRVIAVDA
VPNEVGQTWEPVDVHQETIAYLQYTSGSTRIPTGVQITHLNLATNVVQVIEALEGEEGDRGVSWLPFFHDMGLITIMISP
MIGHYISFMTPAAFVRRPGRWIREMARKEGETGGVISVAPNFAFDHAAARGVPREGEPALDLSNVKALLNGSEPISAATV
RNFNEAFGPFGFKPQAIKPSYGLAEATLFVSTTPVNESPKIVTVDRDELNSHRFVEVPPDSPKAVAQASAGKVGVAEWAV
IVDHDTATELPDGQIGEIWISGQNMGTGYWGKEEETQATFRNILKSRTNPSHAEGAPDDAMWVRTGDLGAFHDGDLYITG
RVKDLVIIDGRNHYPQDLEYSAQEATKALRTGFVAAFSVPANQLPDEVFDNAHAGLKRDPSDTSEQLVIVGERAPGAHKL
DMGPIADDIRAAIAVRHGVTVRDVLLTPAGAIPRTSSGKIGRRACRSAYLDGSLRSGKVANAFPDETD

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=575, Percent_Identity=25.2173913043478, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI44888818, Length=587, Percent_Identity=23.679727427598, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI225735629, Length=644, Percent_Identity=22.0496894409938, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI45827692, Length=644, Percent_Identity=22.0496894409938, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI225735625, Length=644, Percent_Identity=22.0496894409938, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI45827694, Length=544, Percent_Identity=22.2426470588235, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI225735627, Length=525, Percent_Identity=21.7142857142857, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45827696, Length=525, Percent_Identity=21.7142857142857, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788107, Length=597, Percent_Identity=24.7906197654941, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI193202679, Length=584, Percent_Identity=23.972602739726, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=481, Percent_Identity=24.5322245322245, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24654656, Length=475, Percent_Identity=22.7368421052632, Blast_Score=97, Evalue=5e-20,
Organism=Drosophila melanogaster, GI18859661, Length=588, Percent_Identity=23.9795918367347, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24581924, Length=435, Percent_Identity=23.6781609195402, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 68608; Mature: 68477

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFHNPFIKDGLIKFPDNGNLVRHVERWAKVRGDKLAYRFIDFSTERDGVERDLTWADFG
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEECCC
GRNRAVGARLQQVTQPGDRVAILCPQNLDYLVAFFGTLYSGRIAVPLFDPNEPGHVGRLH
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHH
AVLDDCTPSAILTTTEAAEGVRKFFRTRPANQRPRVIAVDAVPNEVGQTWEPVDVHQETI
HHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCCCCCCCHHHHHEE
AYLQYTSGSTRIPTGVQITHLNLATNVVQVIEALEGEEGDRGVSWLPFFHDMGLITIMIS
EEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEECCHHHHCCEEEHHHH
PMIGHYISFMTPAAFVRRPGRWIREMARKEGETGGVISVAPNFAFDHAAARGVPREGEPA
HHHHHHHHHHCHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCCCCCC
LDLSNVKALLNGSEPISAATVRNFNEAFGPFGFKPQAIKPSYGLAEATLFVSTTPVNESP
CCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCC
KIVTVDRDELNSHRFVEVPPDSPKAVAQASAGKVGVAEWAVIVDHDTATELPDGQIGEIW
CEEEEEHHHCCCCEEEECCCCCCHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEE
ISGQNMGTGYWGKEEETQATFRNILKSRTNPSHAEGAPDDAMWVRTGDLGAFHDGDLYIT
EECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEECCCEEEE
GRVKDLVIIDGRNHYPQDLEYSAQEATKALRTGFVAAFSVPANQLPDEVFDNAHAGLKRD
ECEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHHCCHHHHHCCCCCCCCC
PSDTSEQLVIVGERAPGAHKLDMGPIADDIRAAIAVRHGVTVRDVLLTPAGAIPRTSSGK
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHEECCCCCCCCCCCCC
IGRRACRSAYLDGSLRSGKVANAFPDETD
HHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PFHNPFIKDGLIKFPDNGNLVRHVERWAKVRGDKLAYRFIDFSTERDGVERDLTWADFG
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEECCC
GRNRAVGARLQQVTQPGDRVAILCPQNLDYLVAFFGTLYSGRIAVPLFDPNEPGHVGRLH
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHH
AVLDDCTPSAILTTTEAAEGVRKFFRTRPANQRPRVIAVDAVPNEVGQTWEPVDVHQETI
HHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCCCCCCCHHHHHEE
AYLQYTSGSTRIPTGVQITHLNLATNVVQVIEALEGEEGDRGVSWLPFFHDMGLITIMIS
EEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEECCHHHHCCEEEHHHH
PMIGHYISFMTPAAFVRRPGRWIREMARKEGETGGVISVAPNFAFDHAAARGVPREGEPA
HHHHHHHHHHCHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCCCCCC
LDLSNVKALLNGSEPISAATVRNFNEAFGPFGFKPQAIKPSYGLAEATLFVSTTPVNESP
CCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCC
KIVTVDRDELNSHRFVEVPPDSPKAVAQASAGKVGVAEWAVIVDHDTATELPDGQIGEIW
CEEEEEHHHCCCCEEEECCCCCCHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEE
ISGQNMGTGYWGKEEETQATFRNILKSRTNPSHAEGAPDDAMWVRTGDLGAFHDGDLYIT
EECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEECCCEEEE
GRVKDLVIIDGRNHYPQDLEYSAQEATKALRTGFVAAFSVPANQLPDEVFDNAHAGLKRD
ECEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHHCCHHHHHCCCCCCCCC
PSDTSEQLVIVGERAPGAHKLDMGPIADDIRAAIAVRHGVTVRDVLLTPAGAIPRTSSGK
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHEECCCCCCCCCCCCC
IGRRACRSAYLDGSLRSGKVANAFPDETD
HHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]