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The map label for this gene is nth [H]

Identifier: 108801779

GI number: 108801779

Start: 5100408

End: 5101187

Strand: Reverse

Name: nth [H]

Synonym: Mmcs_4816

Alternate gene names: 108801779

Gene position: 5101187-5100408 (Counterclockwise)

Preceding gene: 108801782

Following gene: 108801778

Centisome position: 89.41

GC content: 67.18

Gene sequence:

>780_bases
GTGACCGCAGGTACGGCCGACCCGAAGCCGGCGCGGCGTGCTGCCGCCAAGAAGTGGGACAACGAGACGCAGCTGGGCCT
GGTGCGGCGCGCGCGACGGATGAATCGTGCTCTGGCCCAAGCGTTTCCACACGTGTACTGCGAGCTCGACTTCACCAATC
CGCTCGAACTCGCGGTGGCCACCATTCTGTCCGCCCAGAGCACCGACAAGCGGGTCAACCTGACCACCCCCGCGTTGTTC
AAGAAGTACCGCACCGCACTCGACTACGCCCAGGCCGACCGCACCGAACTCGAGGAGTTGATCCGGCCCACCGGTTTCTA
CCGGAACAAGGCCAATTCGCTGATCCGGCTCGGCCAGGAACTCGTCGAACGCTTCGACGGTCAGGTGCCCGCCGACATCG
ACGACCTGGTGACGCTGCCGGGGGTGGGCCGCAAAACGGCCAACGTCATCCTCGGCAACGCCTTCGAGGTCCCAGGAATC
ACCGTCGACACCCACTTCGGCCGGTTGGTGCGCCGGTGGCGGTGGACCGCCGAAGAGGATCCGGTGAAGGTCGAGCATGC
GATCGGCAAACTCATCGAGCGCAGCGAATGGACCCTGCTGAGCCATCGCGTGATCTTCCACGGCCGCCGCGTATGCCACG
CCCGCAAGCCGGCCTGTGGGGTGTGCGTACTGGCCAAGGACTGCCCGTCGTTCGGCATCGGCCCGACCGACCGCGAAGCC
GCCGCGGCGCTGGTCAAGGGGCCGGAGACCGAGCACCTACTCGCCCTCGCGGGCCTGTAG

Upstream 100 bases:

>100_bases
AAGTAAACCGGGCCAAGGTCTCAGCGTGATCACGAATCGCCACCGGTATCGAGCACCTTGATCACGGTCGCCGTCAGGGC
TCTCGCCTAGTCTGGTTGCC

Downstream 100 bases:

>100_bases
CCCGACCCTTCTCCCACCATGAGCACCTCGACCCGCTGGACTGTCGCCGTGCTGGCCGTGGTGCTCGCGCTGGTGGTGGC
GCTGTCGATGCAGCTGGCCG

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTAGTADPKPARRAAAKKWDNETQLGLVRRARRMNRALAQAFPHVYCELDFTNPLELAVATILSAQSTDKRVNLTTPALF
KKYRTALDYAQADRTELEELIRPTGFYRNKANSLIRLGQELVERFDGQVPADIDDLVTLPGVGRKTANVILGNAFEVPGI
TVDTHFGRLVRRWRWTAEEDPVKVEHAIGKLIERSEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGIGPTDREA
AAALVKGPETEHLLALAGL

Sequences:

>Translated_259_residues
MTAGTADPKPARRAAAKKWDNETQLGLVRRARRMNRALAQAFPHVYCELDFTNPLELAVATILSAQSTDKRVNLTTPALF
KKYRTALDYAQADRTELEELIRPTGFYRNKANSLIRLGQELVERFDGQVPADIDDLVTLPGVGRKTANVILGNAFEVPGI
TVDTHFGRLVRRWRWTAEEDPVKVEHAIGKLIERSEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGIGPTDREA
AAALVKGPETEHLLALAGL
>Mature_258_residues
TAGTADPKPARRAAAKKWDNETQLGLVRRARRMNRALAQAFPHVYCELDFTNPLELAVATILSAQSTDKRVNLTTPALFK
KYRTALDYAQADRTELEELIRPTGFYRNKANSLIRLGQELVERFDGQVPADIDDLVTLPGVGRKTANVILGNAFEVPGIT
VDTHFGRLVRRWRWTAEEDPVKVEHAIGKLIERSEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGIGPTDREAA
AALVKGPETEHLLALAGL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=219, Percent_Identity=28.7671232876712, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1787920, Length=191, Percent_Identity=38.2198952879581, Blast_Score=134, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=33.7078651685393, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=31.5789473684211, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319304, Length=191, Percent_Identity=27.2251308900524, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550361, Length=175, Percent_Identity=33.7142857142857, Blast_Score=104, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 28768; Mature: 28637

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGTADPKPARRAAAKKWDNETQLGLVRRARRMNRALAQAFPHVYCELDFTNPLELAVA
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHH
TILSAQSTDKRVNLTTPALFKKYRTALDYAQADRTELEELIRPTGFYRNKANSLIRLGQE
HHHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVERFDGQVPADIDDLVTLPGVGRKTANVILGNAFEVPGITVDTHFGRLVRRWRWTAEED
HHHHHCCCCCCCHHHHHCCCCCCCHHHHEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCC
PVKVEHAIGKLIERSEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGIGPTDREA
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHH
AAALVKGPETEHLLALAGL
HHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure 
TAGTADPKPARRAAAKKWDNETQLGLVRRARRMNRALAQAFPHVYCELDFTNPLELAVA
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHH
TILSAQSTDKRVNLTTPALFKKYRTALDYAQADRTELEELIRPTGFYRNKANSLIRLGQE
HHHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVERFDGQVPADIDDLVTLPGVGRKTANVILGNAFEVPGITVDTHFGRLVRRWRWTAEED
HHHHHCCCCCCCHHHHHCCCCCCCHHHHEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCC
PVKVEHAIGKLIERSEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGIGPTDREA
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHH
AAALVKGPETEHLLALAGL
HHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]