Click here to switch to the map view.

The map label for this gene is est [H]

Identifier: 108801114

GI number: 108801114

Start: 4415940

End: 4416992

Strand: Reverse

Name: est [H]

Synonym: Mmcs_4150

Alternate gene names: 108801114

Gene position: 4416992-4415940 (Counterclockwise)

Preceding gene: 108801117

Following gene: 108801113

Centisome position: 77.42

GC content: 70.09

Gene sequence:

>1053_bases
ATGACGGCACCGAGTAGGGTCCCGAGCTCCTCGTATGCTGGCCTCCGGGCGGGTCGACCGCAGAAGCCGCGGCGCTTCCC
GGTCAGCGACGGCGCACCGGTCGAGGTGGTCGAAGACGGCCCGAGTATCCCCGCGCGACTCATGTCGCTGGCGGCGATGA
TGACGATCAAGCCCACGCTGACGATCGGTAGCCTCGCACCGAAGATGCCCTGGCCGTGGGGTGCCGTCGACTTTGCCTGC
CGCGCCCTGCGCCCGGCCCCGGGCACCGTCCGCGCCACCATCGGCCTGCAGAACTGCACCGCCCAGCTGGTGCGGGCGCC
CGGCGTCCTGCCGGCCGACGGCAAGCGCAGCGTGATCCTCTACATGCACGGCGGCGCTTTCCTGACGTGTGGCGTCAACT
CGCACAGCCGGCTCGTGACGGCGCTGTCGAAGTTCGCTGACAGCCCGGCGCTGGTCGTCAACTACCGGATGATCCCCAAG
CATTCGGTGGGTCAGGCCGTCGACGACTGCTACGACGCCTACCTGTGGCTGCGCCGCAAGGGCTACGAACCCGACCAGAT
CGTGCTGGCCGGTGACTCGGCGGGTGGATACCTGTCGCTGGCCCTCGCCGAGCGGTTGCAGCTCGAGGACGAGACCCCCG
CGGCGGTGGTCACCATGTCCCCGCTGTTCGAGCTCGACAACGAGGCCCGCGCCAACCACCCGAACGTCCGCAGCGACGCG
ATGTTCCCGCAGCGGGCGTTCCACGCCCTGGTCGAGCTCATCGAGCACGCCGCCGAGAAGCATCTCGTCGACGGCCGTCC
CGAGCCGGTGTACGAACCGCTCGACCACATCGAACCCGGGCTGCCGCGCACGCTGATCCACGTGTCCGGCTCCGAGGTGC
TCATCAGCGACGCGCGTAAGGCGGCGCGTCTGCTGGCCGCCGCCGGCGTCCCGGTCGAGGTCCGGGTGTGGCCCGGGCAG
ATGCACGTCTTCCAGCTCGGGGCGCCGCTGGTGTCGGAGGCGCAGCGTTCGCTGCGCCAGATCGGTGAGTACATACGAGA
GGCCACCTGGTAG

Upstream 100 bases:

>100_bases
AGACCATTGTGATATCGGTTACAAGTGCGAAGCTGAGTTACGGAGATCGGCTGAGTAGCTGAGCACACCTGGCACTACAA
CGGCGTAGAAGGTGGTGGCG

Downstream 100 bases:

>100_bases
TCGTTCAGCGCGCCTGGGACGATGGAGTCATGCGCATCGCCCAGCACGTCAGTGAGCTCATCGGCAACACCCCTCTGGTA
CAGCTGAATTCTGTTGTCCC

Product: alpha/beta hydrolase fold-3

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTAPSRVPSSSYAGLRAGRPQKPRRFPVSDGAPVEVVEDGPSIPARLMSLAAMMTIKPTLTIGSLAPKMPWPWGAVDFAC
RALRPAPGTVRATIGLQNCTAQLVRAPGVLPADGKRSVILYMHGGAFLTCGVNSHSRLVTALSKFADSPALVVNYRMIPK
HSVGQAVDDCYDAYLWLRRKGYEPDQIVLAGDSAGGYLSLALAERLQLEDETPAAVVTMSPLFELDNEARANHPNVRSDA
MFPQRAFHALVELIEHAAEKHLVDGRPEPVYEPLDHIEPGLPRTLIHVSGSEVLISDARKAARLLAAAGVPVEVRVWPGQ
MHVFQLGAPLVSEAQRSLRQIGEYIREATW

Sequences:

>Translated_350_residues
MTAPSRVPSSSYAGLRAGRPQKPRRFPVSDGAPVEVVEDGPSIPARLMSLAAMMTIKPTLTIGSLAPKMPWPWGAVDFAC
RALRPAPGTVRATIGLQNCTAQLVRAPGVLPADGKRSVILYMHGGAFLTCGVNSHSRLVTALSKFADSPALVVNYRMIPK
HSVGQAVDDCYDAYLWLRRKGYEPDQIVLAGDSAGGYLSLALAERLQLEDETPAAVVTMSPLFELDNEARANHPNVRSDA
MFPQRAFHALVELIEHAAEKHLVDGRPEPVYEPLDHIEPGLPRTLIHVSGSEVLISDARKAARLLAAAGVPVEVRVWPGQ
MHVFQLGAPLVSEAQRSLRQIGEYIREATW
>Mature_349_residues
TAPSRVPSSSYAGLRAGRPQKPRRFPVSDGAPVEVVEDGPSIPARLMSLAAMMTIKPTLTIGSLAPKMPWPWGAVDFACR
ALRPAPGTVRATIGLQNCTAQLVRAPGVLPADGKRSVILYMHGGAFLTCGVNSHSRLVTALSKFADSPALVVNYRMIPKH
SVGQAVDDCYDAYLWLRRKGYEPDQIVLAGDSAGGYLSLALAERLQLEDETPAAVVTMSPLFELDNEARANHPNVRSDAM
FPQRAFHALVELIEHAAEKHLVDGRPEPVYEPLDHIEPGLPRTLIHVSGSEVLISDARKAARLLAAAGVPVEVRVWPGQM
HVFQLGAPLVSEAQRSLRQIGEYIREATW

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=237, Percent_Identity=23.6286919831224, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 37950; Mature: 37819

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSRVPSSSYAGLRAGRPQKPRRFPVSDGAPVEVVEDGPSIPARLMSLAAMMTIKPTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCE
TIGSLAPKMPWPWGAVDFACRALRPAPGTVRATIGLQNCTAQLVRAPGVLPADGKRSVIL
EECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCEEEE
YMHGGAFLTCGVNSHSRLVTALSKFADSPALVVNYRMIPKHSVGQAVDDCYDAYLWLRRK
EEECCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GYEPDQIVLAGDSAGGYLSLALAERLQLEDETPAAVVTMSPLFELDNEARANHPNVRSDA
CCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCC
MFPQRAFHALVELIEHAAEKHLVDGRPEPVYEPLDHIEPGLPRTLIHVSGSEVLISDARK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCCEEEHHHHHH
AARLLAAAGVPVEVRVWPGQMHVFQLGAPLVSEAQRSLRQIGEYIREATW
HHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAPSRVPSSSYAGLRAGRPQKPRRFPVSDGAPVEVVEDGPSIPARLMSLAAMMTIKPTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCE
TIGSLAPKMPWPWGAVDFACRALRPAPGTVRATIGLQNCTAQLVRAPGVLPADGKRSVIL
EECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCEEEE
YMHGGAFLTCGVNSHSRLVTALSKFADSPALVVNYRMIPKHSVGQAVDDCYDAYLWLRRK
EEECCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GYEPDQIVLAGDSAGGYLSLALAERLQLEDETPAAVVTMSPLFELDNEARANHPNVRSDA
CCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCC
MFPQRAFHALVELIEHAAEKHLVDGRPEPVYEPLDHIEPGLPRTLIHVSGSEVLISDARK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCCEEEHHHHHH
AARLLAAAGVPVEVRVWPGQMHVFQLGAPLVSEAQRSLRQIGEYIREATW
HHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]