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The map label for this gene is emrB [C]

Identifier: 108800934

GI number: 108800934

Start: 4232814

End: 4234754

Strand: Reverse

Name: emrB [C]

Synonym: Mmcs_3970

Alternate gene names: 108800934

Gene position: 4234754-4232814 (Counterclockwise)

Preceding gene: 108800935

Following gene: 108800931

Centisome position: 74.22

GC content: 70.43

Gene sequence:

>1941_bases
ATGTTCTCCACGTCCGAAGCGCGACCGGTGACCGGTGACCCATGGCATGCGCTGTGGGCCATGATGGTCGGCTTCTTCAT
GATCCTGGTCGACGCCACCATCGTGGCGGTCGCCAACCCGGTGCTCATGGAGAAGCTGGGCGCCGACTACGACGCGGTGA
TCTGGGTGACCAGCGCCTATCTGCTGGCCTACGCGGTCCCGCTGCTGGTAGCGGGTCGGCTGGGGGACCGGTTCGGGCCC
AAGAACGTCTACCTGGTCGGTCTGGCCGTATTCACCGCCGCCTCGCTGTGGTGCGGGCTGGCCGGGTCCATCGACACGCT
GATCGCCGCACGGGTCGTCCAGGGCGTCGGCGCGGCACTGCTGACGCCGCAGACCCTGTCGACGATCACCCGCATCTTCC
CGCCCGAGCGGCGCGGTGTGGCGATGAGTGTGTGGGGTGCGACGGCCGGGGTGGCCACGCTGGTGGGGCCGCTGGCGGGT
GGCGTGCTGGTGGACCACCTCGGTTGGCAGTGGATCTTCTTCGTCAACGTGCCGGTCGGGGTGGCGGGCCTGGCGTTGGC
GTTCTGGCGGGTGCCCGCGCTGACCACCACCGCGCACCGGTTCGACCTGCTCGGGGTGCTGCTGTCCGGGGTCGGGATGT
TCGCGATCGTCTTCGGGCTGCAGGAGGGGCAGTCCCACGGCTGGCAGCCCTGGATCTGGGTGGTCATCGTGGCCGGCGTC
GCGGTGATGGCCGGGTTCGTCTACTGGCAGTCGGTCAACCCCCACGAACCGTTGATCCCGTTGCGGATCTTCGGTGACCG
CGACTTCTCGCTGTCCAGCTTCGGCGTCGCCGTCATCGGCTTCGTGGTGACCGGCATGATCGTGCCCGCGATGTTCTTCG
CCCAGGCGGTGTGTGGGCTGTCGCCGACCGAGTCGGCGCTGCTGACCGCGCCGATGGCCATCACCAGTGGAGTGCTGGCA
CCCGCGGTCGGGCGAATCGTCGACCGCGCCCATCCGCGACCGATCGTCGGGTTCGGGTTCTCGGCGCTGGCGATCGGGCT
GACCTGGTTGTCGATCGAGATGACGCCGGATACGCCGATCTGGCGGCTGGTCCTGCCGTTCCTCGTGATGGGTATCGGTA
TGGCGTTCATCTGGTCGCCGCTGGCGGCCACCGCGACCCGGAATCTGGCACCGCATCTCGCCGGCGCCGGGTCCGGCGTC
TACAACGCGACGCGTCAGGTCGGGTCGGTGCTGGGCAGCGCCGGCATGGCGGCGTTCATGACGTCGCGGATCAGCGCGGA
GATGCCGTCGGCGCAGGGCGGGGCGCCGCGCGGGGAGGGGGCGGTGTCGGCGCTCCCCGAATTCCTTCAGGTGCCGTTCG
CCGCCGCGATGTCGCAGTCGCTGCTGCTGCCGGCGTTCGTGGCGTTGATCGGGGTGGTCGCGGCGATCTTCCTGCGCGGC
TTCGGCGAGGTATCGGCGCCGGTTGTCGCCGCGCCGGTGGCGCGCGCCGACCCCCGGGACGACTCCGTCGACGACAGCCA
CGGCTATGACGACGACGACTACCTCGAGTACGCCGTCAGCTGGGACGATCTTCAGTTCACCGAACCCATCTCGACCCGTC
CTGAGGTCGGCGCCGACGACAGTGTCACGACGCCGCTCGCAACGCGGGGCCGCCGCGCGGAGGCGGCCGCCGAGCCGTCC
GGTGCCGACGATCCGTGGCGCCGTGTGCTCGACGAACTGCTACCGGAGCCGCCGGCCCGGCCCGAGGCCGAGCCGATCGG
CTTCGCGCACAACGGTTTCCATGTGGAGGGAGAGGAGACGCCGGTCGACGACAGACGGGGCCGGCGATACCGAGACGACG
GCGACGCTGCACCCGCATGGCTGCGCGAGTTCGGTGAACGCTCGCGCCGCGGAACGGACACCCCCTCCGGCGGCCGTCAT
TCACGCCGCGACGGCGACTGA

Upstream 100 bases:

>100_bases
GAACCCGCCTGAGAACCCGGCCACCTTTGGACTTGTTTCCTCGGGTGCCGGGTACGTAGCGTCGGCTGGCTGGATCCTTC
GATAGGCGAGTCGACCCGAT

Downstream 100 bases:

>100_bases
CTCAACGACAGGAACGCGCCGAGTTCGACCAGTCCCTTCGGCGTGGAAGGCAGGTACTCGGTGAGGGCCTCCGAGCGCAC
GATCACGGCGGCGTACTTCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MFSTSEARPVTGDPWHALWAMMVGFFMILVDATIVAVANPVLMEKLGADYDAVIWVTSAYLLAYAVPLLVAGRLGDRFGP
KNVYLVGLAVFTAASLWCGLAGSIDTLIAARVVQGVGAALLTPQTLSTITRIFPPERRGVAMSVWGATAGVATLVGPLAG
GVLVDHLGWQWIFFVNVPVGVAGLALAFWRVPALTTTAHRFDLLGVLLSGVGMFAIVFGLQEGQSHGWQPWIWVVIVAGV
AVMAGFVYWQSVNPHEPLIPLRIFGDRDFSLSSFGVAVIGFVVTGMIVPAMFFAQAVCGLSPTESALLTAPMAITSGVLA
PAVGRIVDRAHPRPIVGFGFSALAIGLTWLSIEMTPDTPIWRLVLPFLVMGIGMAFIWSPLAATATRNLAPHLAGAGSGV
YNATRQVGSVLGSAGMAAFMTSRISAEMPSAQGGAPRGEGAVSALPEFLQVPFAAAMSQSLLLPAFVALIGVVAAIFLRG
FGEVSAPVVAAPVARADPRDDSVDDSHGYDDDDYLEYAVSWDDLQFTEPISTRPEVGADDSVTTPLATRGRRAEAAAEPS
GADDPWRRVLDELLPEPPARPEAEPIGFAHNGFHVEGEETPVDDRRGRRYRDDGDAAPAWLREFGERSRRGTDTPSGGRH
SRRDGD

Sequences:

>Translated_646_residues
MFSTSEARPVTGDPWHALWAMMVGFFMILVDATIVAVANPVLMEKLGADYDAVIWVTSAYLLAYAVPLLVAGRLGDRFGP
KNVYLVGLAVFTAASLWCGLAGSIDTLIAARVVQGVGAALLTPQTLSTITRIFPPERRGVAMSVWGATAGVATLVGPLAG
GVLVDHLGWQWIFFVNVPVGVAGLALAFWRVPALTTTAHRFDLLGVLLSGVGMFAIVFGLQEGQSHGWQPWIWVVIVAGV
AVMAGFVYWQSVNPHEPLIPLRIFGDRDFSLSSFGVAVIGFVVTGMIVPAMFFAQAVCGLSPTESALLTAPMAITSGVLA
PAVGRIVDRAHPRPIVGFGFSALAIGLTWLSIEMTPDTPIWRLVLPFLVMGIGMAFIWSPLAATATRNLAPHLAGAGSGV
YNATRQVGSVLGSAGMAAFMTSRISAEMPSAQGGAPRGEGAVSALPEFLQVPFAAAMSQSLLLPAFVALIGVVAAIFLRG
FGEVSAPVVAAPVARADPRDDSVDDSHGYDDDDYLEYAVSWDDLQFTEPISTRPEVGADDSVTTPLATRGRRAEAAAEPS
GADDPWRRVLDELLPEPPARPEAEPIGFAHNGFHVEGEETPVDDRRGRRYRDDGDAAPAWLREFGERSRRGTDTPSGGRH
SRRDGD
>Mature_646_residues
MFSTSEARPVTGDPWHALWAMMVGFFMILVDATIVAVANPVLMEKLGADYDAVIWVTSAYLLAYAVPLLVAGRLGDRFGP
KNVYLVGLAVFTAASLWCGLAGSIDTLIAARVVQGVGAALLTPQTLSTITRIFPPERRGVAMSVWGATAGVATLVGPLAG
GVLVDHLGWQWIFFVNVPVGVAGLALAFWRVPALTTTAHRFDLLGVLLSGVGMFAIVFGLQEGQSHGWQPWIWVVIVAGV
AVMAGFVYWQSVNPHEPLIPLRIFGDRDFSLSSFGVAVIGFVVTGMIVPAMFFAQAVCGLSPTESALLTAPMAITSGVLA
PAVGRIVDRAHPRPIVGFGFSALAIGLTWLSIEMTPDTPIWRLVLPFLVMGIGMAFIWSPLAATATRNLAPHLAGAGSGV
YNATRQVGSVLGSAGMAAFMTSRISAEMPSAQGGAPRGEGAVSALPEFLQVPFAAAMSQSLLLPAFVALIGVVAAIFLRG
FGEVSAPVVAAPVARADPRDDSVDDSHGYDDDDYLEYAVSWDDLQFTEPISTRPEVGADDSVTTPLATRGRRAEAAAEPS
GADDPWRRVLDELLPEPPARPEAEPIGFAHNGFHVEGEETPVDDRRGRRYRDDGDAAPAWLREFGERSRRGTDTPSGGRH
SRRDGD

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=435, Percent_Identity=28.2758620689655, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788710, Length=424, Percent_Identity=29.2452830188679, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1790195, Length=428, Percent_Identity=29.2056074766355, Blast_Score=162, Evalue=8e-41,
Organism=Escherichia coli, GI1788392, Length=400, Percent_Identity=32, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=28.9156626506024, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6322958, Length=422, Percent_Identity=23.4597156398104, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=217, Percent_Identity=26.2672811059908, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=391, Percent_Identity=24.8081841432225, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=155, Percent_Identity=30.9677419354839, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323520, Length=238, Percent_Identity=27.3109243697479, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=436, Percent_Identity=22.4770642201835, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=347, Percent_Identity=23.342939481268, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 68518; Mature: 68518

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSTSEARPVTGDPWHALWAMMVGFFMILVDATIVAVANPVLMEKLGADYDAVIWVTSAY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHH
LLAYAVPLLVAGRLGDRFGPKNVYLVGLAVFTAASLWCGLAGSIDTLIAARVVQGVGAAL
HHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
LTPQTLSTITRIFPPERRGVAMSVWGATAGVATLVGPLAGGVLVDHLGWQWIFFVNVPVG
CCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHH
VAGLALAFWRVPALTTTAHRFDLLGVLLSGVGMFAIVFGLQEGQSHGWQPWIWVVIVAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AVMAGFVYWQSVNPHEPLIPLRIFGDRDFSLSSFGVAVIGFVVTGMIVPAMFFAQAVCGL
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPTESALLTAPMAITSGVLAPAVGRIVDRAHPRPIVGFGFSALAIGLTWLSIEMTPDTPI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHEEEEEEECCCCCH
WRLVLPFLVMGIGMAFIWSPLAATATRNLAPHLAGAGSGVYNATRQVGSVLGSAGMAAFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TSRISAEMPSAQGGAPRGEGAVSALPEFLQVPFAAAMSQSLLLPAFVALIGVVAAIFLRG
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGEVSAPVVAAPVARADPRDDSVDDSHGYDDDDYLEYAVSWDDLQFTEPISTRPEVGADD
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCCC
SVTTPLATRGRRAEAAAEPSGADDPWRRVLDELLPEPPARPEAEPIGFAHNGFHVEGEET
CCCCCHHHCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCC
PVDDRRGRRYRDDGDAAPAWLREFGERSRRGTDTPSGGRHSRRDGD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFSTSEARPVTGDPWHALWAMMVGFFMILVDATIVAVANPVLMEKLGADYDAVIWVTSAY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHH
LLAYAVPLLVAGRLGDRFGPKNVYLVGLAVFTAASLWCGLAGSIDTLIAARVVQGVGAAL
HHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
LTPQTLSTITRIFPPERRGVAMSVWGATAGVATLVGPLAGGVLVDHLGWQWIFFVNVPVG
CCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHH
VAGLALAFWRVPALTTTAHRFDLLGVLLSGVGMFAIVFGLQEGQSHGWQPWIWVVIVAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AVMAGFVYWQSVNPHEPLIPLRIFGDRDFSLSSFGVAVIGFVVTGMIVPAMFFAQAVCGL
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPTESALLTAPMAITSGVLAPAVGRIVDRAHPRPIVGFGFSALAIGLTWLSIEMTPDTPI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHEEEEEEECCCCCH
WRLVLPFLVMGIGMAFIWSPLAATATRNLAPHLAGAGSGVYNATRQVGSVLGSAGMAAFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TSRISAEMPSAQGGAPRGEGAVSALPEFLQVPFAAAMSQSLLLPAFVALIGVVAAIFLRG
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGEVSAPVVAAPVARADPRDDSVDDSHGYDDDDYLEYAVSWDDLQFTEPISTRPEVGADD
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCCC
SVTTPLATRGRRAEAAAEPSGADDPWRRVLDELLPEPPARPEAEPIGFAHNGFHVEGEET
CCCCCHHHCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCC
PVDDRRGRRYRDDGDAAPAWLREFGERSRRGTDTPSGGRHSRRDGD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]