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The map label for this gene is fadE25 [H]

Identifier: 108800783

GI number: 108800783

Start: 4065009

End: 4066055

Strand: Reverse

Name: fadE25 [H]

Synonym: Mmcs_3819

Alternate gene names: 108800783

Gene position: 4066055-4065009 (Counterclockwise)

Preceding gene: 108800784

Following gene: 108800782

Centisome position: 71.27

GC content: 67.91

Gene sequence:

>1047_bases
TTGAGGAACTGGCTGACCGACAACGCCAAGGCCTTCGCGGCCTCCGGTGACGACTACTGGGCGCGGCAGGGGGAGTGGCA
TCAGGCGCTGTACTCCGCCGGGTTCTTCGGCACCTCGTGGCCGCGCGAGTTCGGCGGGCAGGACCTCCCGCCGGTCTACG
ACGTCATCGTCGACGAGGAGCTCGCACGCGCCGGCGCCCCGCCGCGGCCCAGCCTGGGTTACCTCGTCGTCGGCCTGGGA
CACCACGGCAGCAAGGAACTGCAGCAGCGGTTCCTGCCGGGGATGATCAACGGCACCGAGCGTTGGTGCCAGGGCTTCAG
CGAACCGGGCGCGGGCTCGGACCTGGCGTCCTTGACCACCACCGCCACGCGCGACGGTGACAACTACCTCATCCACGGCC
ACAAGATCTGGACGAGCTACTCCGACATCGCCGACTGGTGTCTGGTGCTCGCCCGCACGGACAAGGAAGTGCCGCGGCAC
AAGGGCATCTCGGCGTTCATCGTCGACATGCACCAGCCGGGGATCGAACAGCGACCGCTGAAGATGATCAACGGCGTGAC
AACCGAATTCGGACAGGTGGCATTCGACGGCGCCGTGGTGCCCGCCGACCAGATGATCGGCGCGCCGGGGGAGGGTTGGG
CGCTGGCGATGACGGTCGTCAGCCACGAACGCGAACCGTCGACGCTGGGCTATTCGGCCAGGTACGGAAAGCTGGTGCGG
CAGATGGCCGCCCGGGTCGACGGCCGGCCGCCGGAGGATCTGGTGTGGGCGGCGGTCGAGGCCGAGATGCTGCGGCTGCA
CGTCCGCCGCCGGCTGTCCGAGCAGCTCGACGGCATCACCCACGGCCCGCAGGGCTCGCTGGACAAGCTGCTGATGACGT
GGACCGAACAGTCGGTGGGCCACGCCGCGCTCTCGGTCAGCGGAACCAAGGATCCGGACCTGTTGAGCGCCTACCTCTAC
AGTCGCGCGCAAAGCGTGATGGGTGGGACGTCGCAGATCCAGAAGAACATCATCGCGTCACGAATCCTGGGACTGGGAGT
GCGATGA

Upstream 100 bases:

>100_bases
CGGCGCGTTCTGACTTCGACCGAATTGTGGCCGGTACGTCTGGAGGACATCGATCTTGGATTTTCGTGATTCACCCGAAG
AGACAGCCTTCCGGGACAGG

Downstream 100 bases:

>100_bases
GCGCCCGCGCGAAGAGCAGAGGAGCCTGACATGTACGACATGCCTGACGAAATCCAAGTGACCGCGGACGGTCCGCTGCG
GATCATCACGCTGAACCGGC

Product: acyl-CoA dehydrogenase-like protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MRNWLTDNAKAFAASGDDYWARQGEWHQALYSAGFFGTSWPREFGGQDLPPVYDVIVDEELARAGAPPRPSLGYLVVGLG
HHGSKELQQRFLPGMINGTERWCQGFSEPGAGSDLASLTTTATRDGDNYLIHGHKIWTSYSDIADWCLVLARTDKEVPRH
KGISAFIVDMHQPGIEQRPLKMINGVTTEFGQVAFDGAVVPADQMIGAPGEGWALAMTVVSHEREPSTLGYSARYGKLVR
QMAARVDGRPPEDLVWAAVEAEMLRLHVRRRLSEQLDGITHGPQGSLDKLLMTWTEQSVGHAALSVSGTKDPDLLSAYLY
SRAQSVMGGTSQIQKNIIASRILGLGVR

Sequences:

>Translated_348_residues
MRNWLTDNAKAFAASGDDYWARQGEWHQALYSAGFFGTSWPREFGGQDLPPVYDVIVDEELARAGAPPRPSLGYLVVGLG
HHGSKELQQRFLPGMINGTERWCQGFSEPGAGSDLASLTTTATRDGDNYLIHGHKIWTSYSDIADWCLVLARTDKEVPRH
KGISAFIVDMHQPGIEQRPLKMINGVTTEFGQVAFDGAVVPADQMIGAPGEGWALAMTVVSHEREPSTLGYSARYGKLVR
QMAARVDGRPPEDLVWAAVEAEMLRLHVRRRLSEQLDGITHGPQGSLDKLLMTWTEQSVGHAALSVSGTKDPDLLSAYLY
SRAQSVMGGTSQIQKNIIASRILGLGVR
>Mature_348_residues
MRNWLTDNAKAFAASGDDYWARQGEWHQALYSAGFFGTSWPREFGGQDLPPVYDVIVDEELARAGAPPRPSLGYLVVGLG
HHGSKELQQRFLPGMINGTERWCQGFSEPGAGSDLASLTTTATRDGDNYLIHGHKIWTSYSDIADWCLVLARTDKEVPRH
KGISAFIVDMHQPGIEQRPLKMINGVTTEFGQVAFDGAVVPADQMIGAPGEGWALAMTVVSHEREPSTLGYSARYGKLVR
QMAARVDGRPPEDLVWAAVEAEMLRLHVRRRLSEQLDGITHGPQGSLDKLLMTWTEQSVGHAALSVSGTKDPDLLSAYLY
SRAQSVMGGTSQIQKNIIASRILGLGVR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958414, Length=209, Percent_Identity=30.622009569378, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI226958412, Length=206, Percent_Identity=29.6116504854369, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI4557233, Length=334, Percent_Identity=26.9461077844311, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4501857, Length=357, Percent_Identity=25.4901960784314, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI187960098, Length=340, Percent_Identity=25.5882352941176, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI4557231, Length=340, Percent_Identity=25.5882352941176, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI4501859, Length=205, Percent_Identity=29.7560975609756, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI21361497, Length=209, Percent_Identity=25.8373205741627, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4557235, Length=212, Percent_Identity=29.2452830188679, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI76496475, Length=233, Percent_Identity=28.3261802575107, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI48976061, Length=314, Percent_Identity=25.796178343949, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI211971008, Length=314, Percent_Identity=25.796178343949, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI7656849, Length=235, Percent_Identity=26.8085106382979, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786223, Length=222, Percent_Identity=28.8288288288288, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081958, Length=338, Percent_Identity=25.4437869822485, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538396, Length=213, Percent_Identity=30.5164319248826, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17570075, Length=367, Percent_Identity=25.0681198910082, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17534899, Length=227, Percent_Identity=31.7180616740088, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17569725, Length=371, Percent_Identity=25.0673854447439, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17506239, Length=367, Percent_Identity=26.4305177111717, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551932, Length=236, Percent_Identity=27.5423728813559, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508101, Length=375, Percent_Identity=24.8, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17505929, Length=216, Percent_Identity=28.2407407407407, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32563615, Length=197, Percent_Identity=31.4720812182741, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985184, Length=160, Percent_Identity=30.625, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71985192, Length=160, Percent_Identity=30.625, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71990804, Length=203, Percent_Identity=28.0788177339901, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982178, Length=154, Percent_Identity=29.8701298701299, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17533517, Length=354, Percent_Identity=23.728813559322, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646207, Length=336, Percent_Identity=27.6785714285714, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356377, Length=336, Percent_Identity=27.6785714285714, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24666513, Length=204, Percent_Identity=31.8627450980392, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24660351, Length=206, Percent_Identity=29.6116504854369, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355753, Length=205, Percent_Identity=31.219512195122, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281363737, Length=234, Percent_Identity=26.0683760683761, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 38112; Mature: 38112

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNWLTDNAKAFAASGDDYWARQGEWHQALYSAGFFGTSWPREFGGQDLPPVYDVIVDEE
CCCCCCCCCCEEECCCCHHHHCCCHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHH
LARAGAPPRPSLGYLVVGLGHHGSKELQQRFLPGMINGTERWCQGFSEPGAGSDLASLTT
HHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHH
TATRDGDNYLIHGHKIWTSYSDIADWCLVLARTDKEVPRHKGISAFIVDMHQPGIEQRPL
HHCCCCCCEEEECCEECCCHHHHHHHHHHHHHCCHHCHHHCCCEEEEEECCCCCCCCCHH
KMINGVTTEFGQVAFDGAVVPADQMIGAPGEGWALAMTVVSHEREPSTLGYSARYGKLVR
HHHHCCHHHHHHHEECCCCCCHHHHCCCCCCCCEEEEEEHHCCCCCCCCCCHHHHHHHHH
QMAARVDGRPPEDLVWAAVEAEMLRLHVRRRLSEQLDGITHGPQGSLDKLLMTWTEQSVG
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
HAALSVSGTKDPDLLSAYLYSRAQSVMGGTSQIQKNIIASRILGLGVR
CEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRNWLTDNAKAFAASGDDYWARQGEWHQALYSAGFFGTSWPREFGGQDLPPVYDVIVDEE
CCCCCCCCCCEEECCCCHHHHCCCHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHH
LARAGAPPRPSLGYLVVGLGHHGSKELQQRFLPGMINGTERWCQGFSEPGAGSDLASLTT
HHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHH
TATRDGDNYLIHGHKIWTSYSDIADWCLVLARTDKEVPRHKGISAFIVDMHQPGIEQRPL
HHCCCCCCEEEECCEECCCHHHHHHHHHHHHHCCHHCHHHCCCEEEEEECCCCCCCCCHH
KMINGVTTEFGQVAFDGAVVPADQMIGAPGEGWALAMTVVSHEREPSTLGYSARYGKLVR
HHHHCCHHHHHHHEECCCCCCHHHHCCCCCCCCEEEEEEHHCCCCCCCCCCHHHHHHHHH
QMAARVDGRPPEDLVWAAVEAEMLRLHVRRRLSEQLDGITHGPQGSLDKLLMTWTEQSVG
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
HAALSVSGTKDPDLLSAYLYSRAQSVMGGTSQIQKNIIASRILGLGVR
CEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]