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The map label for this gene is kshB [H]

Identifier: 108800677

GI number: 108800677

Start: 3964776

End: 3965795

Strand: Reverse

Name: kshB [H]

Synonym: Mmcs_3712

Alternate gene names: 108800677

Gene position: 3965795-3964776 (Counterclockwise)

Preceding gene: 108800679

Following gene: 108800676

Centisome position: 69.51

GC content: 66.86

Gene sequence:

>1020_bases
ATGTCCGAACCAGTCGCCGATTTCTCGCCGCTGCGCGTCAAGCGGGTCGTACGTGAGACCTCTGATGCGGTGTCGCTGGT
GCTCGACGTGCCCCGCCACTGCTCCGGGCGGTTCCGCTACCAGGCCGGTCAATTCCTGACGCTGCGGGTCAACCTCGACG
GCCAGGAGTTGCGTCGCTGCTATTCGATGTCGTCGGCTCCGGTCGAGGAGGAACTCCGGATCACCGTCAAGCGCGATCCC
GGCGGACTCGTGTCGAACTGGCTGAACGACACCGTGTCGGAAGGGGCGGAGATCCACGCCGCGCCGCCGGAGGGTCGGTT
CCTGTTGCGCGACACCGACTCCGAGATTGTCGCCTTCGCCGGCGGTAGCGGGATCACGCCGATCATGTCGCTGATCCGCA
CAGCGCTCGCGACGTCGGCGCGGTCCATCCGGCTGTTCTATGCCAACCGCAACCGGGGATCGGTCATCTTCGCCGATGAC
CTGGCTCGCCTCGTCGAACACAACGCCGACCGACTGGTGCTGACACACCATTACGACGACGACAGCGGCGTCGTCACCCC
TGCGGCGATCGAATCCTTCGTGGCAGGCGCCGGAGATGCCGATTTCTACATCTGCGGACCGGGACCGTTCATGGACACGG
TGGAGGGAGCACTTGCGACGAGGGGCGCACCCCGCGACCGCGTGCACCTCGAGCGCTTCCAGGTGGCGCAGGTGCCCGCC
GGCGCAGACGCATCCGAGGCGCAGGCCACCGAAGAGATCGTCATCGAACTGGACCGCAAGACCACCACCGTGCCCTACCG
GTCCGGCAACACCCTGCTGCAGACCGCGCGCACGGCCGGTCTGCGCGCACCCTCGTCGTGCGAGACCGGTTCCTGCGGAA
CGTGTATGGCCCAGGTGGTGTCCGGCAGCGCGCGGATGCTCAACAACGATGCGCTCGACGACGACGAGGTGGCCGACGGC
TGGGTGCTGACCTGCCAATCGCTGCCGACGAGTCGATCAGTGCACGTGGTCTACGAGTAG

Upstream 100 bases:

>100_bases
CCATGCGATGACCTGATGATGCGGTGTGTCGTTCACGCCGGTATTCGACCACGGCCGGCCCCTTGATCCCAATGACAAGT
ACTTGATACTGTGGGCCACG

Downstream 100 bases:

>100_bases
AGGTGAAGCGATGACACGGGTAGCGGTGGTGACCGGCGGCGCCTCGGGCATGGGGGAGGCGATCTGTCACGAGCTCGGCA
GGCGCGGACACCGGGTCGCC

Product: ferredoxin

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSEPVADFSPLRVKRVVRETSDAVSLVLDVPRHCSGRFRYQAGQFLTLRVNLDGQELRRCYSMSSAPVEEELRITVKRDP
GGLVSNWLNDTVSEGAEIHAAPPEGRFLLRDTDSEIVAFAGGSGITPIMSLIRTALATSARSIRLFYANRNRGSVIFADD
LARLVEHNADRLVLTHHYDDDSGVVTPAAIESFVAGAGDADFYICGPGPFMDTVEGALATRGAPRDRVHLERFQVAQVPA
GADASEAQATEEIVIELDRKTTTVPYRSGNTLLQTARTAGLRAPSSCETGSCGTCMAQVVSGSARMLNNDALDDDEVADG
WVLTCQSLPTSRSVHVVYE

Sequences:

>Translated_339_residues
MSEPVADFSPLRVKRVVRETSDAVSLVLDVPRHCSGRFRYQAGQFLTLRVNLDGQELRRCYSMSSAPVEEELRITVKRDP
GGLVSNWLNDTVSEGAEIHAAPPEGRFLLRDTDSEIVAFAGGSGITPIMSLIRTALATSARSIRLFYANRNRGSVIFADD
LARLVEHNADRLVLTHHYDDDSGVVTPAAIESFVAGAGDADFYICGPGPFMDTVEGALATRGAPRDRVHLERFQVAQVPA
GADASEAQATEEIVIELDRKTTTVPYRSGNTLLQTARTAGLRAPSSCETGSCGTCMAQVVSGSARMLNNDALDDDEVADG
WVLTCQSLPTSRSVHVVYE
>Mature_338_residues
SEPVADFSPLRVKRVVRETSDAVSLVLDVPRHCSGRFRYQAGQFLTLRVNLDGQELRRCYSMSSAPVEEELRITVKRDPG
GLVSNWLNDTVSEGAEIHAAPPEGRFLLRDTDSEIVAFAGGSGITPIMSLIRTALATSARSIRLFYANRNRGSVIFADDL
ARLVEHNADRLVLTHHYDDDSGVVTPAAIESFVAGAGDADFYICGPGPFMDTVEGALATRGAPRDRVHLERFQVAQVPAG
ADASEAQATEEIVIELDRKTTTVPYRSGNTLLQTARTAGLRAPSSCETGSCGTCMAQVVSGSARMLNNDALDDDEVADGW
VLTCQSLPTSRSVHVVYE

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=350, Percent_Identity=31.7142857142857, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1787098, Length=328, Percent_Identity=28.3536585365854, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788903, Length=214, Percent_Identity=28.9719626168224, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788104, Length=293, Percent_Identity=23.8907849829352, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 36645; Mature: 36514

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPVADFSPLRVKRVVRETSDAVSLVLDVPRHCSGRFRYQAGQFLTLRVNLDGQELRRC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCHHHHHHH
YSMSSAPVEEELRITVKRDPGGLVSNWLNDTVSEGAEIHAAPPEGRFLLRDTDSEIVAFA
HCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCEEEEE
GGSGITPIMSLIRTALATSARSIRLFYANRNRGSVIFADDLARLVEHNADRLVLTHHYDD
CCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEEEECC
DSGVVTPAAIESFVAGAGDADFYICGPGPFMDTVEGALATRGAPRDRVHLERFQVAQVPA
CCCEECHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHEEECCC
GADASEAQATEEIVIELDRKTTTVPYRSGNTLLQTARTAGLRAPSSCETGSCGTCMAQVV
CCCCHHHHHCEEEEEEECCCEEECEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SGSARMLNNDALDDDEVADGWVLTCQSLPTSRSVHVVYE
CCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEC
>Mature Secondary Structure 
SEPVADFSPLRVKRVVRETSDAVSLVLDVPRHCSGRFRYQAGQFLTLRVNLDGQELRRC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCHHHHHHH
YSMSSAPVEEELRITVKRDPGGLVSNWLNDTVSEGAEIHAAPPEGRFLLRDTDSEIVAFA
HCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCEEEEE
GGSGITPIMSLIRTALATSARSIRLFYANRNRGSVIFADDLARLVEHNADRLVLTHHYDD
CCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEEEECC
DSGVVTPAAIESFVAGAGDADFYICGPGPFMDTVEGALATRGAPRDRVHLERFQVAQVPA
CCCEECHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHEEECCC
GADASEAQATEEIVIELDRKTTTVPYRSGNTLLQTARTAGLRAPSSCETGSCGTCMAQVV
CCCCHHHHHCEEEEEEECCCEEECEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SGSARMLNNDALDDDEVADGWVLTCQSLPTSRSVHVVYE
CCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA