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The map label for this gene is tfdA [H]

Identifier: 108800673

GI number: 108800673

Start: 3961517

End: 3962362

Strand: Reverse

Name: tfdA [H]

Synonym: Mmcs_3708

Alternate gene names: 108800673

Gene position: 3962362-3961517 (Counterclockwise)

Preceding gene: 108800674

Following gene: 108800672

Centisome position: 69.45

GC content: 68.2

Gene sequence:

>846_bases
ATGACTGTGCTGACGATCAACAAGCTCACCGCATCTGTCGGCGCGGAGGTGACCGGGCTGGATCCGGATGCGCTGGCCGG
CGACGAGGCACTGGGTGCCGCGGTGCTCGAGGCTCTCGAGGACAACGGTGTGCTGGTCTTCCCGGGTCTGCACCTCGACC
CGCAGGCACAGGTCGAGTTCTGCCGGCGCCTCGGCGAGGTCGACCACTCCTCCGACGGACACCATCCGGTGGCGGGGATC
TATCCGGTCACCCTCGACAAGTCGAAGAACTCCTCGGCGGCCTATCTGCGCGCCACGTTCGACTGGCACATCGACGGGTG
CACCCCGACCGGCGACGAGTACCCACAGATGGCGACGGTGCTCTCGGCGCGGCGGGTGGCCGAGAGTGGCGGGGAGACCG
AGTTCGCCAGTTCCTACGGCGCCTACGATCACCTCGACGACGACGAGAAGCAGCGGTTGGCATCGCTGCGGGTGGTGCAC
TCGCTGGAGGCCTCACAGCGCCGCGTCACCCCCGACCCGTCGCCGGAGTTGTTGGCGCGGTGGCGGTCCCGTCCCACGCA
TGAACATCCCCTCGTCTGGACCCACCGAAGTGGCCGCAAGTCCCTGGTCCTCGGTGCCTCCGCCGACTACATCGTCGGCA
TGGACCTCGACGAGGGCAGGGCCCTGCTGGCCGATCTGCTCGACCGCGCCACGCAACCCGAACTCGTCTACAGCCACACC
TGGTCGGTCGGTGACACCGTCATCTGGGACAACCGCGGCGTACTGCACCGCGCGGCGCCGTATCCGGAGAACTCACCGCG
GGAGATGCTGCGCACCACCGTGCTCGGCGACGAACCGATCCAGTGA

Upstream 100 bases:

>100_bases
GCCGTCGAGATCTTCGGGACGCAGAAACCCACCGACTCCGCCGACGCGGTCATGCATCGCGTCAGCGCGCCGGTGCCATG
ACAACCGGGACGGGACGACC

Downstream 100 bases:

>100_bases
CGCGTCTGACGCCGCTCCCGCCCGACCGCTGGGACGACGACGTCCGGCGGGCGTTGAGCCCGTTGCTCGGCGCCGACCGG
GCGAACCCACGCGATGCGGG

Product: taurine catabolism dioxygenase TauD/TfdA

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTVLTINKLTASVGAEVTGLDPDALAGDEALGAAVLEALEDNGVLVFPGLHLDPQAQVEFCRRLGEVDHSSDGHHPVAGI
YPVTLDKSKNSSAAYLRATFDWHIDGCTPTGDEYPQMATVLSARRVAESGGETEFASSYGAYDHLDDDEKQRLASLRVVH
SLEASQRRVTPDPSPELLARWRSRPTHEHPLVWTHRSGRKSLVLGASADYIVGMDLDEGRALLADLLDRATQPELVYSHT
WSVGDTVIWDNRGVLHRAAPYPENSPREMLRTTVLGDEPIQ

Sequences:

>Translated_281_residues
MTVLTINKLTASVGAEVTGLDPDALAGDEALGAAVLEALEDNGVLVFPGLHLDPQAQVEFCRRLGEVDHSSDGHHPVAGI
YPVTLDKSKNSSAAYLRATFDWHIDGCTPTGDEYPQMATVLSARRVAESGGETEFASSYGAYDHLDDDEKQRLASLRVVH
SLEASQRRVTPDPSPELLARWRSRPTHEHPLVWTHRSGRKSLVLGASADYIVGMDLDEGRALLADLLDRATQPELVYSHT
WSVGDTVIWDNRGVLHRAAPYPENSPREMLRTTVLGDEPIQ
>Mature_280_residues
TVLTINKLTASVGAEVTGLDPDALAGDEALGAAVLEALEDNGVLVFPGLHLDPQAQVEFCRRLGEVDHSSDGHHPVAGIY
PVTLDKSKNSSAAYLRATFDWHIDGCTPTGDEYPQMATVLSARRVAESGGETEFASSYGAYDHLDDDEKQRLASLRVVHS
LEASQRRVTPDPSPELLARWRSRPTHEHPLVWTHRSGRKSLVLGASADYIVGMDLDEGRALLADLLDRATQPELVYSHTW
SVGDTVIWDNRGVLHRAAPYPENSPREMLRTTVLGDEPIQ

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=286, Percent_Identity=29.3706293706294, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322971, Length=302, Percent_Identity=27.1523178807947, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 30735; Mature: 30604

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLTINKLTASVGAEVTGLDPDALAGDEALGAAVLEALEDNGVLVFPGLHLDPQAQVEF
CEEEEEEHHHHHHCCEEECCCCHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
CRRLGEVDHSSDGHHPVAGIYPVTLDKSKNSSAAYLRATFDWHIDGCTPTGDEYPQMATV
HHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHH
LSARRVAESGGETEFASSYGAYDHLDDDEKQRLASLRVVHSLEASQRRVTPDPSPELLAR
HHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
WRSRPTHEHPLVWTHRSGRKSLVLGASADYIVGMDLDEGRALLADLLDRATQPELVYSHT
HHCCCCCCCCEEEEECCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEC
WSVGDTVIWDNRGVLHRAAPYPENSPREMLRTTVLGDEPIQ
CCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TVLTINKLTASVGAEVTGLDPDALAGDEALGAAVLEALEDNGVLVFPGLHLDPQAQVEF
EEEEEEHHHHHHCCEEECCCCHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
CRRLGEVDHSSDGHHPVAGIYPVTLDKSKNSSAAYLRATFDWHIDGCTPTGDEYPQMATV
HHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHH
LSARRVAESGGETEFASSYGAYDHLDDDEKQRLASLRVVHSLEASQRRVTPDPSPELLAR
HHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
WRSRPTHEHPLVWTHRSGRKSLVLGASADYIVGMDLDEGRALLADLLDRATQPELVYSHT
HHCCCCCCCCEEEEECCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEC
WSVGDTVIWDNRGVLHRAAPYPENSPREMLRTTVLGDEPIQ
CCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8779585 [H]