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The map label for this gene is vhcA [H]

Identifier: 108800382

GI number: 108800382

Start: 3625549

End: 3626841

Strand: Reverse

Name: vhcA [H]

Synonym: Mmcs_3416

Alternate gene names: 108800382

Gene position: 3626841-3625549 (Counterclockwise)

Preceding gene: 108800383

Following gene: 108800381

Centisome position: 63.57

GC content: 68.83

Gene sequence:

>1293_bases
GTGAATCCCGAGATCCGCACCCTGACTGTCGGCGCGCTGACCCGTGTGGAAGGTGAAGGGGCGCTGCACGTCACGTTGAC
CGACGGCGTGGTGGAAAGCGTCGAACTGAACATCTACGAGCCGCCGCGGTTCTTCGAGGCGTTCCTGCGGGGACGGGCCT
ACACCGAACCGCCCGACATCACCGCGCGGGTCTGCGGCATCTGCCCGGTGGCCTATCAGGTCAGTGCCTGCAACGCGATC
GAGGATGCGTGCGGCGTGACGCTCGATCCCGAACTGATCGAGCTGCGGCGGCTGCTGTACTGCGGGGAGTGGATCCACAG
TCACGCCCTGCACATCTATCTGCTGCACGCACCGGACTTCCTGGGATACCCGGACGCCATCAGCTTCGCCAAGGACCACC
GAGAGTTCCTCGAACGCGGGCTGGCCCTGAAGAAGGCCGGGAACCGGCTGATGGAGCAGATCGGCGGGCGGGCCATCCAC
CCGATCAACGTCCGATTGGGCGGCTTCTACTCGGTTCCGCGCCGCGACGAGCTCGGACCGCTGGCCGAGGAACTGCGCCG
CGCGCTGGACGACGCCCTGTTCACGCTGCGGTCGGTGGCCGAATTCGACTTCCCCGACGTGGAATTCGACCACGAGTTCC
TCGCGCTGACGCAACCGGACCGGTACGCGATCGAGGACGGCACGATCGTGCGCAGCGCCGGGCCCGCATTCCCGGTGGCC
GACTTCAGCGAGCACGTCAGGGAGATGCAGGTGCCGCACTCCACGGCGCTGCACGCGACGCTCGACGGGGAGCGCTATCT
GACCGGGCCGCTCGCGCGGTACTCGCTGAGCTCGGCCGCCCTCTCACCGACCGCCCGGCAGGCCGCCGCACAGGCGGGGC
TGGGTGCGCAGTGCCGAAACCCGTTCCGCAGCATCGTCGTCCGTGCTGTGGAGGTCGTGTACGCGATCGAGGAGGCGCTG
CGGATCATCGAGGACTACGAGCGGCCCGCCCGGCCGTTCGTCGACGTGCCGGCCCGCGCCGGCGTCGGCCACGGCGTCAG
CGAGGCGCCGCGCGGGCTGCTGTACCACCGCTACGAGATCGGCGACGACGGTCTGGTCAGCGCGGCGACGATGGTGCCAC
CGACCGCGCAGAACCAGGCCGCCATCGAATACGAGATGGGCCGGCTGGTGGCCGGCAACCTCACGCTCGACGATGCGGAG
TTGACGTCGTTGTGCGAGCGGTCGATCCGCAACCACGACCCGTGCATCTCGTGTTCGGCGCACTTCCTGACGCTCACCGT
CGAGCGCGGATGA

Upstream 100 bases:

>100_bases
TCATCCCGGTGCTGCGCCGTGACGGGATGTCGGGCGGTGACGTCGACCGGGTGTTCTCGACGTTCAACGTCGCGAAGTTC
GCCGAGCACCGGGACGATAC

Downstream 100 bases:

>100_bases
GCGACATCGTCGTGATCGGCGTCGGCAACAGCTGTCGCCGGGACGACGGTGTGGGCCCGGCCGTCGCTTCGGCCGTCGAT
GCCCGCGGGACTCCCGGCGT

Product: nickel-dependent hydrogenase, large subunit

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNPEIRTLTVGALTRVEGEGALHVTLTDGVVESVELNIYEPPRFFEAFLRGRAYTEPPDITARVCGICPVAYQVSACNAI
EDACGVTLDPELIELRRLLYCGEWIHSHALHIYLLHAPDFLGYPDAISFAKDHREFLERGLALKKAGNRLMEQIGGRAIH
PINVRLGGFYSVPRRDELGPLAEELRRALDDALFTLRSVAEFDFPDVEFDHEFLALTQPDRYAIEDGTIVRSAGPAFPVA
DFSEHVREMQVPHSTALHATLDGERYLTGPLARYSLSSAALSPTARQAAAQAGLGAQCRNPFRSIVVRAVEVVYAIEEAL
RIIEDYERPARPFVDVPARAGVGHGVSEAPRGLLYHRYEIGDDGLVSAATMVPPTAQNQAAIEYEMGRLVAGNLTLDDAE
LTSLCERSIRNHDPCISCSAHFLTLTVERG

Sequences:

>Translated_430_residues
MNPEIRTLTVGALTRVEGEGALHVTLTDGVVESVELNIYEPPRFFEAFLRGRAYTEPPDITARVCGICPVAYQVSACNAI
EDACGVTLDPELIELRRLLYCGEWIHSHALHIYLLHAPDFLGYPDAISFAKDHREFLERGLALKKAGNRLMEQIGGRAIH
PINVRLGGFYSVPRRDELGPLAEELRRALDDALFTLRSVAEFDFPDVEFDHEFLALTQPDRYAIEDGTIVRSAGPAFPVA
DFSEHVREMQVPHSTALHATLDGERYLTGPLARYSLSSAALSPTARQAAAQAGLGAQCRNPFRSIVVRAVEVVYAIEEAL
RIIEDYERPARPFVDVPARAGVGHGVSEAPRGLLYHRYEIGDDGLVSAATMVPPTAQNQAAIEYEMGRLVAGNLTLDDAE
LTSLCERSIRNHDPCISCSAHFLTLTVERG
>Mature_430_residues
MNPEIRTLTVGALTRVEGEGALHVTLTDGVVESVELNIYEPPRFFEAFLRGRAYTEPPDITARVCGICPVAYQVSACNAI
EDACGVTLDPELIELRRLLYCGEWIHSHALHIYLLHAPDFLGYPDAISFAKDHREFLERGLALKKAGNRLMEQIGGRAIH
PINVRLGGFYSVPRRDELGPLAEELRRALDDALFTLRSVAEFDFPDVEFDHEFLALTQPDRYAIEDGTIVRSAGPAFPVA
DFSEHVREMQVPHSTALHATLDGERYLTGPLARYSLSSAALSPTARQAAAQAGLGAQCRNPFRSIVVRAVEVVYAIEEAL
RIIEDYERPARPFVDVPARAGVGHGVSEAPRGLLYHRYEIGDDGLVSAATMVPPTAQNQAAIEYEMGRLVAGNLTLDDAE
LTSLCERSIRNHDPCISCSAHFLTLTVERG

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=128, Percent_Identity=28.125, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 47327; Mature: 47327

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEIRTLTVGALTRVEGEGALHVTLTDGVVESVELNIYEPPRFFEAFLRGRAYTEPPDI
CCCCEEEEEECCEEEECCCCEEEEEEECCCEEEEEEEEECCHHHHHHHHCCCCCCCCCCC
TARVCGICPVAYQVSACNAIEDACGVTLDPELIELRRLLYCGEWIHSHALHIYLLHAPDF
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
LGYPDAISFAKDHREFLERGLALKKAGNRLMEQIGGRAIHPINVRLGGFYSVPRRDELGP
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEECEEEEEECCEECCCCCCCCCH
LAEELRRALDDALFTLRSVAEFDFPDVEFDHEFLALTQPDRYAIEDGTIVRSAGPAFPVA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEECCCEEEECCCCCCCHH
DFSEHVREMQVPHSTALHATLDGERYLTGPLARYSLSSAALSPTARQAAAQAGLGAQCRN
HHHHHHHHHCCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHCC
PFRSIVVRAVEVVYAIEEALRIIEDYERPARPFVDVPARAGVGHGVSEAPRGLLYHRYEI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
GDDGLVSAATMVPPTAQNQAAIEYEMGRLVAGNLTLDDAELTSLCERSIRNHDPCISCSA
CCCCCEEHHEECCCCCCCCCEEEEECCCEEECCCEECHHHHHHHHHHHHCCCCCCEECCE
HFLTLTVERG
EEEEEEEECC
>Mature Secondary Structure
MNPEIRTLTVGALTRVEGEGALHVTLTDGVVESVELNIYEPPRFFEAFLRGRAYTEPPDI
CCCCEEEEEECCEEEECCCCEEEEEEECCCEEEEEEEEECCHHHHHHHHCCCCCCCCCCC
TARVCGICPVAYQVSACNAIEDACGVTLDPELIELRRLLYCGEWIHSHALHIYLLHAPDF
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
LGYPDAISFAKDHREFLERGLALKKAGNRLMEQIGGRAIHPINVRLGGFYSVPRRDELGP
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEECEEEEEECCEECCCCCCCCCH
LAEELRRALDDALFTLRSVAEFDFPDVEFDHEFLALTQPDRYAIEDGTIVRSAGPAFPVA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEECCCEEEECCCCCCCHH
DFSEHVREMQVPHSTALHATLDGERYLTGPLARYSLSSAALSPTARQAAAQAGLGAQCRN
HHHHHHHHHCCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHCC
PFRSIVVRAVEVVYAIEEALRIIEDYERPARPFVDVPARAGVGHGVSEAPRGLLYHRYEI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
GDDGLVSAATMVPPTAQNQAAIEYEMGRLVAGNLTLDDAELTSLCERSIRNHDPCISCSA
CCCCCEEHHEECCCCCCCCCEEEEECCCEEECCCEECHHHHHHHHHHHHCCCCCCEECCE
HFLTLTVERG
EEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1603063 [H]