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The map label for this gene is mraW [H]

Identifier: 108800230

GI number: 108800230

Start: 3461922

End: 3462896

Strand: Reverse

Name: mraW [H]

Synonym: Mmcs_3264

Alternate gene names: 108800230

Gene position: 3462896-3461922 (Counterclockwise)

Preceding gene: 108800231

Following gene: 108800229

Centisome position: 60.69

GC content: 71.28

Gene sequence:

>975_bases
ATGGAGCACATACCGGTCCTACTGGATCGTTGCGTCGAGCTGCTCACCCCCGCGCTGACCCGCCGCAATCCCGACGGCCG
CGGAGCCGTCCTGGTCGACGCCACGCTCGGCGCGGGTGGCCACGCCCACCGGTTCCTCAGTGACCTGCCCGGGCTGCATC
TCATCGGGCTGGACCGCGACCCGCAGGCGCTGCAGATCGCCGGCGAGCGGCTCGCGCCGTTCGGTGACCGGGTGTCGCTG
GTGCGCACCCGCTACGACGGGATCGACGACGCCCTGGCGCAGACCGGCGTGGCCGAGGTCAGCGGATTCCTCTTCGACCT
CGGGGTGTCCTCGATGCAACTCGACCGCACCGAGCGCGGGTTCTCCTACAGCGCCGACGCCCCGCTGGACATGCGCATGG
ACAGCGACGCTCCGCTCACCGCGGCCGACGTGGTCAACACGTTCGCGGAGAAGGACATCACCCGGATTCTCCGGGAATTC
GGTGAGGAGCGGTTCGCCGCCCGCATCGCCAAGCACATCGTGCGCCGCCGGCCACTGAACACCACCGGTGAGCTGGTCGA
ACTGCTCTACGATGCCATCCCGGCACCCGCCCGGCGGACCGGCGGGCATCCCGCCAAGCGCACCTTCCAGGCCCTGCGGA
TCGCGGTGAACAGTGAACTGGACTCGCTGCGCGCGGCGGTCCCCGCCGCGCTGGCCGCCCTCGAGACCGGCGGCCGGATC
GTCGTGATGGCCTATCAGTCGCTCGAGGACCGCATCGTCAAGACCGAGTTCGCCGCGGCCACCGCGTCGCGCACACCCCC
CGGGCTGCCCGTCGAACTACCCGGCCACGAACCCGAGTTCGTCGCACTCACCCGCGGGGCCGAGCGGGCCACCCCGGAGG
AGATCGAACGCAATCCGCGCAGTGCGCCGGTGCGGTTGCGCGCACTGGAGAAGGTCGCCGGCAGGCCGACAACGGCCAGG
AGGGACGCCCGATGA

Upstream 100 bases:

>100_bases
AGCGTGGCCTCTGTCCGAACCGGCCCTGGCGTACTTCCCCGACGCCAGGTCCGCCTCGGACAGGGACCTCGCTGCAGGGG
CGTACAGCCCGGGGGTGGCG

Downstream 100 bases:

>100_bases
AGGCAAAGCGATCTGCACCCGTGCGCGGTGAGGAGCGAAGGCGGGCGCCCCGCCAGCCGGTCCGCAAGACGGCCCGCCGG
GGTGTCGAGGCGGCTCCGCG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MEHIPVLLDRCVELLTPALTRRNPDGRGAVLVDATLGAGGHAHRFLSDLPGLHLIGLDRDPQALQIAGERLAPFGDRVSL
VRTRYDGIDDALAQTGVAEVSGFLFDLGVSSMQLDRTERGFSYSADAPLDMRMDSDAPLTAADVVNTFAEKDITRILREF
GEERFAARIAKHIVRRRPLNTTGELVELLYDAIPAPARRTGGHPAKRTFQALRIAVNSELDSLRAAVPAALAALETGGRI
VVMAYQSLEDRIVKTEFAAATASRTPPGLPVELPGHEPEFVALTRGAERATPEEIERNPRSAPVRLRALEKVAGRPTTAR
RDAR

Sequences:

>Translated_324_residues
MEHIPVLLDRCVELLTPALTRRNPDGRGAVLVDATLGAGGHAHRFLSDLPGLHLIGLDRDPQALQIAGERLAPFGDRVSL
VRTRYDGIDDALAQTGVAEVSGFLFDLGVSSMQLDRTERGFSYSADAPLDMRMDSDAPLTAADVVNTFAEKDITRILREF
GEERFAARIAKHIVRRRPLNTTGELVELLYDAIPAPARRTGGHPAKRTFQALRIAVNSELDSLRAAVPAALAALETGGRI
VVMAYQSLEDRIVKTEFAAATASRTPPGLPVELPGHEPEFVALTRGAERATPEEIERNPRSAPVRLRALEKVAGRPTTAR
RDAR
>Mature_324_residues
MEHIPVLLDRCVELLTPALTRRNPDGRGAVLVDATLGAGGHAHRFLSDLPGLHLIGLDRDPQALQIAGERLAPFGDRVSL
VRTRYDGIDDALAQTGVAEVSGFLFDLGVSSMQLDRTERGFSYSADAPLDMRMDSDAPLTAADVVNTFAEKDITRILREF
GEERFAARIAKHIVRRRPLNTTGELVELLYDAIPAPARRTGGHPAKRTFQALRIAVNSELDSLRAAVPAALAALETGGRI
VVMAYQSLEDRIVKTEFAAATASRTPPGLPVELPGHEPEFVALTRGAERATPEEIERNPRSAPVRLRALEKVAGRPTTAR
RDAR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=268, Percent_Identity=39.9253731343284, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI165377202, Length=196, Percent_Identity=38.265306122449, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1786270, Length=321, Percent_Identity=39.2523364485981, Blast_Score=185, Evalue=3e-48,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=35.1190476190476, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=35.1190476190476, Blast_Score=174, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35225; Mature: 35225

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHIPVLLDRCVELLTPALTRRNPDGRGAVLVDATLGAGGHAHRFLSDLPGLHLIGLDRD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCC
PQALQIAGERLAPFGDRVSLVRTRYDGIDDALAQTGVAEVSGFLFDLGVSSMQLDRTERG
CHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCC
FSYSADAPLDMRMDSDAPLTAADVVNTFAEKDITRILREFGEERFAARIAKHIVRRRPLN
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTGELVELLYDAIPAPARRTGGHPAKRTFQALRIAVNSELDSLRAAVPAALAALETGGRI
CHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VVMAYQSLEDRIVKTEFAAATASRTPPGLPVELPGHEPEFVALTRGAERATPEEIERNPR
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHHCCCC
SAPVRLRALEKVAGRPTTARRDAR
CCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEHIPVLLDRCVELLTPALTRRNPDGRGAVLVDATLGAGGHAHRFLSDLPGLHLIGLDRD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCC
PQALQIAGERLAPFGDRVSLVRTRYDGIDDALAQTGVAEVSGFLFDLGVSSMQLDRTERG
CHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCC
FSYSADAPLDMRMDSDAPLTAADVVNTFAEKDITRILREFGEERFAARIAKHIVRRRPLN
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTGELVELLYDAIPAPARRTGGHPAKRTFQALRIAVNSELDSLRAAVPAALAALETGGRI
CHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VVMAYQSLEDRIVKTEFAAATASRTPPGLPVELPGHEPEFVALTRGAERATPEEIERNPR
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHHCCCC
SAPVRLRALEKVAGRPTTARRDAR
CCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA