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The map label for this gene is dop [H]

Identifier: 108800097

GI number: 108800097

Start: 3323232

End: 3324785

Strand: Reverse

Name: dop [H]

Synonym: Mmcs_3131

Alternate gene names: 108800097

Gene position: 3324785-3323232 (Counterclockwise)

Preceding gene: 108800098

Following gene: 108800096

Centisome position: 58.27

GC content: 69.5

Gene sequence:

>1554_bases
GTGCCCGGCACCGCTGCCCGCCCCGCCCCGATCCGCGTCACCTACGCTGAGGTCATGCAGCGGATCATCGGAACCGAGGT
CGAATACGGTATCTCCTCACCGTCCGATCCGACCGCCAACCCGATTCTGACGTCGACGCAGGCAGTGCTGGCCTACGCCG
CCGCGGCCGGCATCCAGCGCGCCAAGCGCACCCGGTGGGACTACGAGGTGGAGTCGCCACTGCGCGACGCCCGCGGGTTC
GACCTGAGCCGTGCCTCCGGCCCGCCGCCGGTGGTCGACGCCGACGAGGTGGGCGCGGCCAACATGATCCTGACCAACGG
CGCACGGCTCTACGTCGACCACGCACACCCCGAGTACTCCGCACCCGAGTGCACCGATCCGCTGGACGCGGTGATCTGGG
ACAAGGCCGGGGAGCGGGTGATGGAGGCCGCGGCGCGCCACGTCGCGAGCGTGCCGGGTGCGGCCAAGCTGCAGCTGTAC
AAGAACAACGTGGACGGCAAGGGCGCCTCCTACGGGTCACACGAGAACTACCTGATGAGCCGGCAGACCCCGTTCTCGGC
GGTGATCGCGGGTCTGACCCCGTTCCTGGTGTCACGCCAGGTGGTCACCGGATCGGGCCGCGTCGGTATCGGGCCCTCGG
GTGACGAACCCGGATTCCAGCTGTCCCAGCGCGCCGACTACATCGAGGTCGAGGTCGGGCTGGAGACCACGCTCAAACGC
GGCATCATCAACACCCGCGACGAACCGCACGCCGACGCCGACAAGTACCGGCGCCTGCACGTCATCATCGGCGACGCCAA
CCTCGCCGAGACGTCGACCTATCTCAAGCTCGGTGCCACCTCCCTGGTCCTCGACCTCATCGAGGAGGGAATGGATCTGT
CGGACCTCGCGCTGGCCCGCCCGGTTCACGCCGTGCACGTGATCAGCCGGGATCCGTCGCTGCGGGCGACGGTCGCGATG
GCCGACGGCCGTGAGCTGACCGCACTGGCGCTGCAGCGCATCTACCTCGACCGGGTGGCCAAGCTGGTGGACAGCCGCGA
CCCGGATCCGCGGGCCGCGCACGTCGTCGAGACGTGGGCCGAGGTGCTGGACCTGCTCGAGCGCGACCCGATGGAGTGCG
CCGAGATCCTCGACTGGCCGGCCAAACTGCGGCTGCTCGAAGGGTTCCGGCACCGGGAGAACCTGGGGTGGTCGGCGCCC
CGGCTGCACCTGGTGGATCTGCAGTACTCCGATGTCCGACTCGACAAGGGGCTGTACAACCGACTGGTCGCCCGCGGGTC
GATGAAGCGTCTGGTCACCGAACAGCAGGTGCTCGACGCCGTCGACAGCCCGCCCACCGACACCCGTGCCTACTTCCGCG
GTGAGTGCCTGCGCCGCTTCGGCGCCGACATCGCCGCGGCCAGTTGGGACTCGGTCATCTTCGACCTGGGCGGCGATTCG
CTGGTGCGGATCCCGACGCTGGAACCGCTGCGCGGCAGCAAGGCGCACGTCGGGGCACTGCTCGACTCGGTCGACAGCGC
CGTCCAGCTCGTCGAGCAGCTCACCGCCACATAG

Upstream 100 bases:

>100_bases
GGCGATCGACACCGAGTCGAATCTCGGCCAGTACCTGTAGCGTTCGACCGTCGGCGGCCCGTCACCGGATTCCGGTGGCG
GGCCGCTTCCCGTTTCAGGG

Downstream 100 bases:

>100_bases
GCGTCCATCCCCAACGCCCGGCTATCCCGGGCATTTCGGGCGTCGACCGGTAGTGTGGAGGAACCGGCGGGTGGCTGTGA
GCCCGTCGATGACAAGTGCA

Product: hypothetical protein

Products: NA

Alternate protein names: Deamidase of protein Pup [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MPGTAARPAPIRVTYAEVMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVESPLRDARGF
DLSRASGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLY
KNNVDGKGASYGSHENYLMSRQTPFSAVIAGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRADYIEVEVGLETTLKR
GIINTRDEPHADADKYRRLHVIIGDANLAETSTYLKLGATSLVLDLIEEGMDLSDLALARPVHAVHVISRDPSLRATVAM
ADGRELTALALQRIYLDRVAKLVDSRDPDPRAAHVVETWAEVLDLLERDPMECAEILDWPAKLRLLEGFRHRENLGWSAP
RLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEQQVLDAVDSPPTDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDS
LVRIPTLEPLRGSKAHVGALLDSVDSAVQLVEQLTAT

Sequences:

>Translated_517_residues
MPGTAARPAPIRVTYAEVMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVESPLRDARGF
DLSRASGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLY
KNNVDGKGASYGSHENYLMSRQTPFSAVIAGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRADYIEVEVGLETTLKR
GIINTRDEPHADADKYRRLHVIIGDANLAETSTYLKLGATSLVLDLIEEGMDLSDLALARPVHAVHVISRDPSLRATVAM
ADGRELTALALQRIYLDRVAKLVDSRDPDPRAAHVVETWAEVLDLLERDPMECAEILDWPAKLRLLEGFRHRENLGWSAP
RLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEQQVLDAVDSPPTDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDS
LVRIPTLEPLRGSKAHVGALLDSVDSAVQLVEQLTAT
>Mature_516_residues
PGTAARPAPIRVTYAEVMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVESPLRDARGFD
LSRASGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLYK
NNVDGKGASYGSHENYLMSRQTPFSAVIAGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRADYIEVEVGLETTLKRG
IINTRDEPHADADKYRRLHVIIGDANLAETSTYLKLGATSLVLDLIEEGMDLSDLALARPVHAVHVISRDPSLRATVAMA
DGRELTALALQRIYLDRVAKLVDSRDPDPRAAHVVETWAEVLDLLERDPMECAEILDWPAKLRLLEGFRHRENLGWSAPR
LHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEQQVLDAVDSPPTDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDSL
VRIPTLEPLRGSKAHVGALLDSVDSAVQLVEQLTAT

Specific function: Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022366
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 56335; Mature: 56204

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTAARPAPIRVTYAEVMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQR
CCCCCCCCCCEEEEHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AKRTRWDYEVESPLRDARGFDLSRASGPPPVVDADEVGAANMILTNGARLYVDHAHPEYS
HHHCCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCC
APECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYLMS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEC
RQTPFSAVIAGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRADYIEVEVGLETTLKR
CCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHCCCCEEEEEECHHHHHHH
GIINTRDEPHADADKYRRLHVIIGDANLAETSTYLKLGATSLVLDLIEEGMDLSDLALAR
CCCCCCCCCCCCHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
PVHAVHVISRDPSLRATVAMADGRELTALALQRIYLDRVAKLVDSRDPDPRAAHVVETWA
CCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EVLDLLERDPMECAEILDWPAKLRLLEGFRHRENLGWSAPRLHLVDLQYSDVRLDKGLYN
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHH
RLVARGSMKRLVTEQQVLDAVDSPPTDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDS
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCC
LVRIPTLEPLRGSKAHVGALLDSVDSAVQLVEQLTAT
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PGTAARPAPIRVTYAEVMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQR
CCCCCCCCCEEEEHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AKRTRWDYEVESPLRDARGFDLSRASGPPPVVDADEVGAANMILTNGARLYVDHAHPEYS
HHHCCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCC
APECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYLMS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEC
RQTPFSAVIAGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRADYIEVEVGLETTLKR
CCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHCCCCEEEEEECHHHHHHH
GIINTRDEPHADADKYRRLHVIIGDANLAETSTYLKLGATSLVLDLIEEGMDLSDLALAR
CCCCCCCCCCCCHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
PVHAVHVISRDPSLRATVAMADGRELTALALQRIYLDRVAKLVDSRDPDPRAAHVVETWA
CCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EVLDLLERDPMECAEILDWPAKLRLLEGFRHRENLGWSAPRLHLVDLQYSDVRLDKGLYN
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHH
RLVARGSMKRLVTEQQVLDAVDSPPTDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDS
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCC
LVRIPTLEPLRGSKAHVGALLDSVDSAVQLVEQLTAT
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]