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The map label for this gene is bioB [H]

Identifier: 108800039

GI number: 108800039

Start: 3247893

End: 3248888

Strand: Reverse

Name: bioB [H]

Synonym: Mmcs_3073

Alternate gene names: 108800039

Gene position: 3248888-3247893 (Counterclockwise)

Preceding gene: 108800041

Following gene: 108800038

Centisome position: 56.94

GC content: 68.07

Gene sequence:

>996_bases
GTGAGCGACATTCTGGCGGTGGCGCGCGAACAGGTGCTCGAGCGCGGTGTCGGTCTGAACCAGGACCAGACGCTGCAGGT
GCTGCAACTGCCCGACGATCGTCTCGACGATCTGCTCGCCCTCGCGCACGAAGTCCGGATGGCCTGGTGCGGCCCCGATG
TCGAGGTCGAGGGCATCATCAGCCTCAAGACCGGCGGCTGCCCCGAGGACTGCCACTTCTGTTCACAATCCGGGCTCTTC
GCCTCGCCCGTGCGCAGCGCGTGGCTCGACGTGCCGAGCCTGGTGGAAGCCGCCAAACAGACCGCCAAGACCGGCGCGAC
GGAATTCTGCATCGTCGCCGCCGTCCGCGGCCCCGACGAACGCCTGCTGGCCCAGGTGGCCGCCGGTATCGAGGCGATCC
GCAACGAGGTCGACATCCAGATCGCGTGTTCGCTCGGCATGCTCACCGCCGAGCAGGTCGAACGCCTCTCGGAGATGGGC
GTGCACCGGTACAACCACAACCTCGAGACGGCGCGGTCGTTCTTCACCAACGTGGTGACCACCCACACCTGGGAGGAACG
GTGGGACACCCTGCGCATGGTGCGCGAAGCCGGTATGGAGGTGTGCTGTGGCGGCATCCTCGGGATGGGGGAGACGCTCG
AGCAGCGCGCCGAGTTCGCGGCCAATCTGGCCGAACTCGATCCGCACGAGGTGCCGCTGAACTTCCTCAACCCGCGGCCG
GGCACCCCGTTCGGGGACCTCGAGGTACTTCCTGCCGCCGAGGCGCTCAAGGCTGTGGCCGCCTTCCGGTTGGCGCTGCC
CCGCACCATGCTGCGCTTCGCGGGCGGCCGCGAGATCACCCTCGGTGACCTGGGGGCCAAGAAGGGCATCCTGGGCGGCA
TCAACGCCGTCATCGTCGGCAACTACCTGACCACGCTCGGGCGACCGGCGGAGGCCGATCTCGAACTGCTCGACGACCTG
CAGATGCCGATCAAGGCGCTCAACGCCAGCCTGTGA

Upstream 100 bases:

>100_bases
CGACCACATCGCGTTTGTGCAGTTGCACGCCGCTACCCTAACCTGAACGGTGTTCAATTGTCGGCTCGGCCGACGTGTTC
AAGAGTCGAGGAGGCAACGG

Downstream 100 bases:

>100_bases
TGGCGCCGAAATTCAACGTCTACACTGGCGAACCCGCCGGCGGCGTCGTCCCGACCGCCGCGCAACTGGGTCTGGAACCA
CCGCGGTTCTGCGCCGAGTG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSDILAVAREQVLERGVGLNQDQTLQVLQLPDDRLDDLLALAHEVRMAWCGPDVEVEGIISLKTGGCPEDCHFCSQSGLF
ASPVRSAWLDVPSLVEAAKQTAKTGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTAEQVERLSEMG
VHRYNHNLETARSFFTNVVTTHTWEERWDTLRMVREAGMEVCCGGILGMGETLEQRAEFAANLAELDPHEVPLNFLNPRP
GTPFGDLEVLPAAEALKAVAAFRLALPRTMLRFAGGREITLGDLGAKKGILGGINAVIVGNYLTTLGRPAEADLELLDDL
QMPIKALNASL

Sequences:

>Translated_331_residues
MSDILAVAREQVLERGVGLNQDQTLQVLQLPDDRLDDLLALAHEVRMAWCGPDVEVEGIISLKTGGCPEDCHFCSQSGLF
ASPVRSAWLDVPSLVEAAKQTAKTGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTAEQVERLSEMG
VHRYNHNLETARSFFTNVVTTHTWEERWDTLRMVREAGMEVCCGGILGMGETLEQRAEFAANLAELDPHEVPLNFLNPRP
GTPFGDLEVLPAAEALKAVAAFRLALPRTMLRFAGGREITLGDLGAKKGILGGINAVIVGNYLTTLGRPAEADLELLDDL
QMPIKALNASL
>Mature_330_residues
SDILAVAREQVLERGVGLNQDQTLQVLQLPDDRLDDLLALAHEVRMAWCGPDVEVEGIISLKTGGCPEDCHFCSQSGLFA
SPVRSAWLDVPSLVEAAKQTAKTGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTAEQVERLSEMGV
HRYNHNLETARSFFTNVVTTHTWEERWDTLRMVREAGMEVCCGGILGMGETLEQRAEFAANLAELDPHEVPLNFLNPRPG
TPFGDLEVLPAAEALKAVAAFRLALPRTMLRFAGGREITLGDLGAKKGILGGINAVIVGNYLTTLGRPAEADLELLDDLQ
MPIKALNASL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=303, Percent_Identity=36.3036303630363, Blast_Score=192, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321725, Length=305, Percent_Identity=28.8524590163934, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 35822; Mature: 35690

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDILAVAREQVLERGVGLNQDQTLQVLQLPDDRLDDLLALAHEVRMAWCGPDVEVEGII
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
SLKTGGCPEDCHFCSQSGLFASPVRSAWLDVPSLVEAAKQTAKTGATEFCIVAAVRGPDE
EECCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHH
RLLAQVAAGIEAIRNEVDIQIACSLGMLTAEQVERLSEMGVHRYNHNLETARSFFTNVVT
HHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
THTWEERWDTLRMVREAGMEVCCGGILGMGETLEQRAEFAANLAELDPHEVPLNFLNPRP
CCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GTPFGDLEVLPAAEALKAVAAFRLALPRTMLRFAGGREITLGDLGAKKGILGGINAVIVG
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHH
NYLTTLGRPAEADLELLDDLQMPIKALNASL
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDILAVAREQVLERGVGLNQDQTLQVLQLPDDRLDDLLALAHEVRMAWCGPDVEVEGII
CHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
SLKTGGCPEDCHFCSQSGLFASPVRSAWLDVPSLVEAAKQTAKTGATEFCIVAAVRGPDE
EECCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHH
RLLAQVAAGIEAIRNEVDIQIACSLGMLTAEQVERLSEMGVHRYNHNLETARSFFTNVVT
HHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
THTWEERWDTLRMVREAGMEVCCGGILGMGETLEQRAEFAANLAELDPHEVPLNFLNPRP
CCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GTPFGDLEVLPAAEALKAVAAFRLALPRTMLRFAGGREITLGDLGAKKGILGGINAVIVG
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHH
NYLTTLGRPAEADLELLDDLQMPIKALNASL
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA