Click here to switch to the map view.

The map label for this gene is nadB [H]

Identifier: 108800033

GI number: 108800033

Start: 3242314

End: 3243960

Strand: Reverse

Name: nadB [H]

Synonym: Mmcs_3067

Alternate gene names: 108800033

Gene position: 3243960-3242314 (Counterclockwise)

Preceding gene: 108800034

Following gene: 108800032

Centisome position: 56.86

GC content: 74.01

Gene sequence:

>1647_bases
ATGACCGGCCCCGGGGGCAGAAGCGCAGCGACCGGGGGGAAGGCGGTTCGTCGCGGTTGCGGCGGCACCACACACTGGCA
GCAGCGTGCCGACGTCGTCGTGATCGGCACCGGCGTCGCCGGTCTGGTGGCGGGGCTGGCGGCACATCGCCGAGGCCGCC
GGGTCATCGTGCTGAGCAAGGCCCCCGAACAGGCCGGATCGACGGCCACCGGATACGCCCAGGGCGGGATCGCGGTGGTG
TTGCCCGGCGGCAGGTGCGATGACGCGGATTCGGTCGACGCCCACGTCGCCGACACCCTCACCGCCGGTGGCGGGTTGTG
CGACCCGGAGGCGGTGCGGTCCATCGTCGCCGACGGGTATCGCGGGGTGCGCGATCTCGTCGACGACGGCGCCCGCTTCG
ACGAGGCCACCCCCGGCCGCTGGGCGCTGACGCGTGAGGGCGGACATTCCCGGCGCCGGATCATCCACGCCGGCGGCGAT
GCCACCGGCGCGGAGGTGCAGCGCGCGCTCGACCATGCGGCACGGGTGCTCGACATCCGACACAACCACGTGGCGGTGCG
GGTGCTGCACGACGACGCCGCGGTGACCGGCGTACTGGTCCTCAGCGAGGACGGACCCGGCATCGTGCACGCCCCGGCGG
TGATCCTCGCGACCGGCGGGCTCGGCCACCTCTATGCGGCCACCACGAACCCGGAGGCCTCCACCGGCGACGGCATCGCG
CTGGCGCTGTGGGCCGGCGTACCGGTCAGCGACATCGAGTTCGTCCAGTTCCACCCCACGATGCTGTTCGACGGCTTCAC
CAACGGCAGGCGTCCGCTGATCACCGAGGCGCTGCGCGGAGAGGGCGCGAGACTCGTCGACGCGCACGGTGACCCGGTTA
CCGCTGGTGTGCATCCGCTGGGTGACCTCGCACCGCGCGATGTGGTCGCCGGCGCGATCGACGCGCGGCTCAAGGCGACC
GGCGACCCGTGCGTCTACCTGGACGCCCGCCACCTGGCCGGGATCGCACACCGTTTCCCGACGGTCACCGCCGCCTGCCG
GCAGGCCGGGATCGACCCCGCTCGCCAGCCCATCCCCGTCGTCCCTGGGGCTCATTACAGCTGCGGCGGTGTCGTCACCG
ACGTCGACGGCCGCACGGCGCTACCCGGTCTGTACGCCGCCGGGGAGGTGGCCCGCACCGGGATGCACGGCGCCAACCGG
CTGGCGTCCAACAGCCTGCTCGAAGGGCTGGTCGTCGGGGCGCGCGCGGGCCGAGGTGCCGCCGAACACGCCGCGGCGGC
GGGCCCGACCCGCGCTGTGGCGCCCGAGCGACTCGTGGGCGAGACCCTCGACCGCCGGGAGCTACAGGCGGCGATGAGCC
GCGACGCGTCGGTGGTGCGCGACGCCGAAGGTCTGATGCGGTTGGGCGACGTGCTCGCCAAGGCGCGTGCCCGTCCGCTG
CGCTCCCGCGCCGATGTCGAGGACGCCGCGCTCACCCTGACCGCGCGGGCGGTTTCGGTCGCCGCGCTGCAGCGCGCGGA
ATCGCGGGGGTGCCACCACCGCGCCGACCATCCGGACACCGACCCGGTGCAGGCGGTCAGCGGGGCGGTCGACCTGGACG
AACACGGACACCTACGACTGAAGATGAGGGCGGACGCGTGCTGCTGA

Upstream 100 bases:

>100_bases
CCTACGGCACCGACGAGGTCGACGTCGACCACGAGACCGCCCGGCTGGCCCGGCGCAGCGTGCAGCGGATGATCGAGATC
GGGCAGCCCGGCGGCGGGGA

Downstream 100 bases:

>100_bases
GTCAGCTTTCGGAGACCGAGATCGCCGAAGCGCGCGCGGTCGTCGCGCGCGGACTCGAGGAGGACCTGCGGTACGGGCCG
GACGTCACCACGCAGGCGAC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTGPGGRSAATGGKAVRRGCGGTTHWQQRADVVVIGTGVAGLVAGLAAHRRGRRVIVLSKAPEQAGSTATGYAQGGIAVV
LPGGRCDDADSVDAHVADTLTAGGGLCDPEAVRSIVADGYRGVRDLVDDGARFDEATPGRWALTREGGHSRRRIIHAGGD
ATGAEVQRALDHAARVLDIRHNHVAVRVLHDDAAVTGVLVLSEDGPGIVHAPAVILATGGLGHLYAATTNPEASTGDGIA
LALWAGVPVSDIEFVQFHPTMLFDGFTNGRRPLITEALRGEGARLVDAHGDPVTAGVHPLGDLAPRDVVAGAIDARLKAT
GDPCVYLDARHLAGIAHRFPTVTAACRQAGIDPARQPIPVVPGAHYSCGGVVTDVDGRTALPGLYAAGEVARTGMHGANR
LASNSLLEGLVVGARAGRGAAEHAAAAGPTRAVAPERLVGETLDRRELQAAMSRDASVVRDAEGLMRLGDVLAKARARPL
RSRADVEDAALTLTARAVSVAALQRAESRGCHHRADHPDTDPVQAVSGAVDLDEHGHLRLKMRADACC

Sequences:

>Translated_548_residues
MTGPGGRSAATGGKAVRRGCGGTTHWQQRADVVVIGTGVAGLVAGLAAHRRGRRVIVLSKAPEQAGSTATGYAQGGIAVV
LPGGRCDDADSVDAHVADTLTAGGGLCDPEAVRSIVADGYRGVRDLVDDGARFDEATPGRWALTREGGHSRRRIIHAGGD
ATGAEVQRALDHAARVLDIRHNHVAVRVLHDDAAVTGVLVLSEDGPGIVHAPAVILATGGLGHLYAATTNPEASTGDGIA
LALWAGVPVSDIEFVQFHPTMLFDGFTNGRRPLITEALRGEGARLVDAHGDPVTAGVHPLGDLAPRDVVAGAIDARLKAT
GDPCVYLDARHLAGIAHRFPTVTAACRQAGIDPARQPIPVVPGAHYSCGGVVTDVDGRTALPGLYAAGEVARTGMHGANR
LASNSLLEGLVVGARAGRGAAEHAAAAGPTRAVAPERLVGETLDRRELQAAMSRDASVVRDAEGLMRLGDVLAKARARPL
RSRADVEDAALTLTARAVSVAALQRAESRGCHHRADHPDTDPVQAVSGAVDLDEHGHLRLKMRADACC
>Mature_547_residues
TGPGGRSAATGGKAVRRGCGGTTHWQQRADVVVIGTGVAGLVAGLAAHRRGRRVIVLSKAPEQAGSTATGYAQGGIAVVL
PGGRCDDADSVDAHVADTLTAGGGLCDPEAVRSIVADGYRGVRDLVDDGARFDEATPGRWALTREGGHSRRRIIHAGGDA
TGAEVQRALDHAARVLDIRHNHVAVRVLHDDAAVTGVLVLSEDGPGIVHAPAVILATGGLGHLYAATTNPEASTGDGIAL
ALWAGVPVSDIEFVQFHPTMLFDGFTNGRRPLITEALRGEGARLVDAHGDPVTAGVHPLGDLAPRDVVAGAIDARLKATG
DPCVYLDARHLAGIAHRFPTVTAACRQAGIDPARQPIPVVPGAHYSCGGVVTDVDGRTALPGLYAAGEVARTGMHGANRL
ASNSLLEGLVVGARAGRGAAEHAAAAGPTRAVAPERLVGETLDRRELQAAMSRDASVVRDAEGLMRLGDVLAKARARPLR
SRADVEDAALTLTARAVSVAALQRAESRGCHHRADHPDTDPVQAVSGAVDLDEHGHLRLKMRADACC

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=510, Percent_Identity=30.9803921568627, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1788928, Length=528, Percent_Identity=41.4772727272727, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1786942, Length=570, Percent_Identity=32.4561403508772, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1790597, Length=526, Percent_Identity=32.8897338403042, Blast_Score=192, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17505833, Length=535, Percent_Identity=30.2803738317757, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17550100, Length=510, Percent_Identity=31.1764705882353, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71986328, Length=424, Percent_Identity=27.5943396226415, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322701, Length=575, Percent_Identity=29.9130434782609, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6322416, Length=539, Percent_Identity=30.0556586270872, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322511, Length=503, Percent_Identity=24.0556660039761, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320788, Length=487, Percent_Identity=26.8993839835729, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137288, Length=532, Percent_Identity=31.015037593985, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24655642, Length=532, Percent_Identity=31.015037593985, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24655647, Length=532, Percent_Identity=31.015037593985, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24663005, Length=560, Percent_Identity=29.4642857142857, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56432; Mature: 56301

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPGGRSAATGGKAVRRGCGGTTHWQQRADVVVIGTGVAGLVAGLAAHRRGRRVIVLSK
CCCCCCCCCCCCCHHHHHCCCCCCCHHCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEEC
APEQAGSTATGYAQGGIAVVLPGGRCDDADSVDAHVADTLTAGGGLCDPEAVRSIVADGY
CCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
RGVRDLVDDGARFDEATPGRWALTREGGHSRRRIIHAGGDATGAEVQRALDHAARVLDIR
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEE
HNHVAVRVLHDDAAVTGVLVLSEDGPGIVHAPAVILATGGLGHLYAATTNPEASTGDGIA
CCEEEEEEEECCCCEEEEEEECCCCCCEEECCEEEEEECCCCEEEEEECCCCCCCCCCEE
LALWAGVPVSDIEFVQFHPTMLFDGFTNGRRPLITEALRGEGARLVDAHGDPVTAGVHPL
EEEECCCCCCCCEEEEECCEEEECCCCCCCCHHHHHHHCCCCCEEEECCCCEEECCCCCC
GDLAPRDVVAGAIDARLKATGDPCVYLDARHLAGIAHRFPTVTAACRQAGIDPARQPIPV
CCCCHHHHHHHHHHCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VPGAHYSCGGVVTDVDGRTALPGLYAAGEVARTGMHGANRLASNSLLEGLVVGARAGRGA
CCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCH
AEHAAAAGPTRAVAPERLVGETLDRRELQAAMSRDASVVRDAEGLMRLGDVLAKARARPL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
RSRADVEDAALTLTARAVSVAALQRAESRGCHHRADHPDTDPVQAVSGAVDLDEHGHLRL
HHHCCCCHHHEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEE
KMRADACC
EECCCCCC
>Mature Secondary Structure 
TGPGGRSAATGGKAVRRGCGGTTHWQQRADVVVIGTGVAGLVAGLAAHRRGRRVIVLSK
CCCCCCCCCCCCHHHHHCCCCCCCHHCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEEC
APEQAGSTATGYAQGGIAVVLPGGRCDDADSVDAHVADTLTAGGGLCDPEAVRSIVADGY
CCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
RGVRDLVDDGARFDEATPGRWALTREGGHSRRRIIHAGGDATGAEVQRALDHAARVLDIR
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEE
HNHVAVRVLHDDAAVTGVLVLSEDGPGIVHAPAVILATGGLGHLYAATTNPEASTGDGIA
CCEEEEEEEECCCCEEEEEEECCCCCCEEECCEEEEEECCCCEEEEEECCCCCCCCCCEE
LALWAGVPVSDIEFVQFHPTMLFDGFTNGRRPLITEALRGEGARLVDAHGDPVTAGVHPL
EEEECCCCCCCCEEEEECCEEEECCCCCCCCHHHHHHHCCCCCEEEECCCCEEECCCCCC
GDLAPRDVVAGAIDARLKATGDPCVYLDARHLAGIAHRFPTVTAACRQAGIDPARQPIPV
CCCCHHHHHHHHHHCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VPGAHYSCGGVVTDVDGRTALPGLYAAGEVARTGMHGANRLASNSLLEGLVVGARAGRGA
CCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCH
AEHAAAAGPTRAVAPERLVGETLDRRELQAAMSRDASVVRDAEGLMRLGDVLAKARARPL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
RSRADVEDAALTLTARAVSVAALQRAESRGCHHRADHPDTDPVQAVSGAVDLDEHGHLRL
HHHCCCCHHHEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEE
KMRADACC
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]