Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 108800023

GI number: 108800023

Start: 3233738

End: 3234523

Strand: Reverse

Name: hisF

Synonym: Mmcs_3057

Alternate gene names: 108800023

Gene position: 3234523-3233738 (Counterclockwise)

Preceding gene: 108800024

Following gene: 108800022

Centisome position: 56.69

GC content: 69.85

Gene sequence:

>786_bases
ATGGCCGCCGACAGAGGACTCGCGGTCCGCGTGATCCCGTGCCTCGACGTCGACGCCGGCCGCGTGGTCAAGGGCGTGAA
CTTCGAGAACCTGCGCGACGCCGGTGATCCGGTCGAACTCGCCGCCGTCTATGACGCCGAGGGTGCCGACGAGTTGACCT
TCCTCGACGTCACCGCATCGTCGTCCGGTCGCTCCACGATGCTCGACGTGGTGCGCCGCACCGCAGAACAGGTCTTCATC
CCGTTGACCGTCGGCGGCGGGGTCCGCGCGGTCGCCGACGTCGACGCGTTGCTGCGGGCCGGTGCGGACAAGGTGTCGGT
GAACACCGCGGCCATCGCCCGCCCGGAACTGCTGGCCGAACTCGCCCGTCAGTTCGGCTCCCAGTGCATCGTGTTGTCGG
TGGACGCCAGAACTGTGCCGCAGGGTGAACAGCCCACCCCGTCCGGGTGGGAGGTGACCACCCACGGTGGTCGTCGCGGC
ACCGGGATCGACGCGGTCGAATGGGCAACGCGCGGAGCCGAACTCGGCGTCGGGGAGATCCTGCTGAACTCGATGGACTT
CGACGGCACCAAGGCCGGGTTCGACCTGCCGATGCTGCGCGCGGTCCGCGGTGCGGTCACCGTGCCCGTGATCGCCAGCG
GCGGGGCCGGTGCGGTCGAGCATTTCGCCCCCGCCGTCCACGCCGGCGCCGACGCCGTGTTGGCGGCCAGCGTCTTCCAC
TTCAAGGAACTCACGATCGGTCAGGTCAAGGCGGCGATGGCGGCAGAAGGGATCACGGTGCGGTAG

Upstream 100 bases:

>100_bases
AGTGGACCCCGGATTCGAAGTCGGTGCTGGCCGGCTCCCCGCAGGTGCACGGTGAGATCCTCGAGATCGTGGCGTCGGTC
GGCGCCCCGGAGGAGTTCTG

Downstream 100 bases:

>100_bases
TGGCTCTCGATCCGGAGATCGCCTCGCGGCTCAAACGCAACGCCGACGGTCTGTTCACCGCCGTCGCGCAGGAACGCGGC
ACCGGTCAGGTGCTGATGGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MAADRGLAVRVIPCLDVDAGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRSTMLDVVRRTAEQVFI
PLTVGGGVRAVADVDALLRAGADKVSVNTAAIARPELLAELARQFGSQCIVLSVDARTVPQGEQPTPSGWEVTTHGGRRG
TGIDAVEWATRGAELGVGEILLNSMDFDGTKAGFDLPMLRAVRGAVTVPVIASGGAGAVEHFAPAVHAGADAVLAASVFH
FKELTIGQVKAAMAAEGITVR

Sequences:

>Translated_261_residues
MAADRGLAVRVIPCLDVDAGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRSTMLDVVRRTAEQVFI
PLTVGGGVRAVADVDALLRAGADKVSVNTAAIARPELLAELARQFGSQCIVLSVDARTVPQGEQPTPSGWEVTTHGGRRG
TGIDAVEWATRGAELGVGEILLNSMDFDGTKAGFDLPMLRAVRGAVTVPVIASGGAGAVEHFAPAVHAGADAVLAASVFH
FKELTIGQVKAAMAAEGITVR
>Mature_260_residues
AADRGLAVRVIPCLDVDAGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRSTMLDVVRRTAEQVFIP
LTVGGGVRAVADVDALLRAGADKVSVNTAAIARPELLAELARQFGSQCIVLSVDARTVPQGEQPTPSGWEVTTHGGRRGT
GIDAVEWATRGAELGVGEILLNSMDFDGTKAGFDLPMLRAVRGAVTVPVIASGGAGAVEHFAPAVHAGADAVLAASVFHF
KELTIGQVKAAMAAEGITVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.9143968871595, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI87082028, Length=252, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=37.1069182389937, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_MYCSJ (A3Q125)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001071343.1
- ProteinModelPortal:   A3Q125
- SMR:   A3Q125
- STRING:   A3Q125
- EnsemblBacteria:   EBMYCT00000051079
- GeneID:   4878786
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_3073
- eggNOG:   COG0107
- GeneTree:   EBGT00050000015857
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   A3Q125
- ProtClustDB:   PRK02083
- BioCyc:   MSP164757:MJLS_3073-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26927; Mature: 26796

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: ACT_SITE 16-16 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADRGLAVRVIPCLDVDAGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTAS
CCCCCCCEEEEEEEECCCCCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEEECC
SSGRSTMLDVVRRTAEQVFIPLTVGGGVRAVADVDALLRAGADKVSVNTAAIARPELLAE
CCCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEECHHHHCCHHHHHH
LARQFGSQCIVLSVDARTVPQGEQPTPSGWEVTTHGGRRGTGIDAVEWATRGAELGVGEI
HHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCCCCHHHH
LLNSMDFDGTKAGFDLPMLRAVRGAVTVPVIASGGAGAVEHFAPAVHAGADAVLAASVFH
HHCCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHH
FKELTIGQVKAAMAAEGITVR
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AADRGLAVRVIPCLDVDAGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTAS
CCCCCCEEEEEEEECCCCCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEEECC
SSGRSTMLDVVRRTAEQVFIPLTVGGGVRAVADVDALLRAGADKVSVNTAAIARPELLAE
CCCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEECHHHHCCHHHHHH
LARQFGSQCIVLSVDARTVPQGEQPTPSGWEVTTHGGRRGTGIDAVEWATRGAELGVGEI
HHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCCCCHHHH
LLNSMDFDGTKAGFDLPMLRAVRGAVTVPVIASGGAGAVEHFAPAVHAGADAVLAASVFH
HHCCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHH
FKELTIGQVKAAMAAEGITVR
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA