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The map label for this gene is lip2 [H]

Identifier: 108800021

GI number: 108800021

Start: 3232388

End: 3233380

Strand: Reverse

Name: lip2 [H]

Synonym: Mmcs_3055

Alternate gene names: 108800021

Gene position: 3233380-3232388 (Counterclockwise)

Preceding gene: 108800022

Following gene: 108800019

Centisome position: 56.67

GC content: 69.49

Gene sequence:

>993_bases
GTGAAGAGGTCGGCGGCGGCCGTCATCGGCGGGGCCACGGCTGCGGTCGCGGCCGGGCGGTATCTTTTGGCACGAGAAGC
GCTGAGTGCCGTTGCACGCGATCTGCGGAGCCCGGTCCTGCCGTACGTCGCCGCGCCGTCGAGCCGGCGATCGATGTTGG
TGACGCGCGCGTTCACGCGGCTGCCCACTCCGGCCGGTGACGGTGTCACGGTGACGGAGTCACGAGTCGGTGAAACTGCG
GTGCGCGTCGTGATCACCGCTCCGGCCGGAGGCGGTACGTCACGGCCCGCGGTGATGTGGATCCACAGCGGCGGGTTCGT
CGTCGGCTCGCCGCAACTGGAGCGGTTCATCACGGGCCAGATCGCACGCGAACTCGGTGCCACCGTGGTCTCGCCGGACT
ATCGACTGGCGCCTGAACACGCTGTCCCAGCCGCGCTCGACGACTGCATGGCGACGTTGAGGTGGATGCGCACCAACGCC
GACCGGCTCGGTATCGACGCCGACCGGATCGTGGTCGGCGGCGCGAGCGCCGGGGGTGGTCTGGCGGCGACGGTCGCCCA
GCGCAGCCTCGACGAAGGATGTCCGCTGCGCGGGCAGGCGCTCGCGTATCCCATGCTCGACGACCGCAGTGTGCTTCGCG
CCGACCATGGCGGCCGTGGGCGATTCGTGTGGACACCCGCGTCCAACCGCTTCGGCTGGACCGCCTATCTCGGACATCCG
CCCGACACCTCGGAGGCGCCCGCCTATGCCGCGGCGGCTCGCAGGTCAGAACTCAGTGGTCTGGCGCCCGCATGGATCGG
CGTCGGCCAGCTCGACCTGCTTCACGACGACTGCGTCTCCTACGCCGAGCGCCTCGAATCCAGCGGCGTGCCATGCGAAC
TCATCACGGTCCCTGGCATGTATCACGCCGCCGACGCAATCGCGGCACGGGCACTGTCCATGCAGACCTTTTGCGCCAGC
ATGCTGAACTTCATGCGCGCGCGACTGTCCTAG

Upstream 100 bases:

>100_bases
CTGCTCGAGGTGGAGCAGTCCGGTCCGGCCTGCCACACCGGCACACACACCTGCTTCGACGCCGACGTGCTGTTGGCCCC
GGAGGCTTAAGCTTCCCGGT

Downstream 100 bases:

>100_bases
GCGGACTGGCGCTCGCGCAACACCTTCAGCGCCTTGTCGGCGTGGGCCTCCATGTTCAGCTCGCTGGAGAACACCTCGAG
CACCGTGCGGTCGGTGTCGA

Product: alpha/beta hydrolase fold-3

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKRSAAAVIGGATAAVAAGRYLLAREALSAVARDLRSPVLPYVAAPSSRRSMLVTRAFTRLPTPAGDGVTVTESRVGETA
VRVVITAPAGGGTSRPAVMWIHSGGFVVGSPQLERFITGQIARELGATVVSPDYRLAPEHAVPAALDDCMATLRWMRTNA
DRLGIDADRIVVGGASAGGGLAATVAQRSLDEGCPLRGQALAYPMLDDRSVLRADHGGRGRFVWTPASNRFGWTAYLGHP
PDTSEAPAYAAAARRSELSGLAPAWIGVGQLDLLHDDCVSYAERLESSGVPCELITVPGMYHAADAIAARALSMQTFCAS
MLNFMRARLS

Sequences:

>Translated_330_residues
MKRSAAAVIGGATAAVAAGRYLLAREALSAVARDLRSPVLPYVAAPSSRRSMLVTRAFTRLPTPAGDGVTVTESRVGETA
VRVVITAPAGGGTSRPAVMWIHSGGFVVGSPQLERFITGQIARELGATVVSPDYRLAPEHAVPAALDDCMATLRWMRTNA
DRLGIDADRIVVGGASAGGGLAATVAQRSLDEGCPLRGQALAYPMLDDRSVLRADHGGRGRFVWTPASNRFGWTAYLGHP
PDTSEAPAYAAAARRSELSGLAPAWIGVGQLDLLHDDCVSYAERLESSGVPCELITVPGMYHAADAIAARALSMQTFCAS
MLNFMRARLS
>Mature_330_residues
MKRSAAAVIGGATAAVAAGRYLLAREALSAVARDLRSPVLPYVAAPSSRRSMLVTRAFTRLPTPAGDGVTVTESRVGETA
VRVVITAPAGGGTSRPAVMWIHSGGFVVGSPQLERFITGQIARELGATVVSPDYRLAPEHAVPAALDDCMATLRWMRTNA
DRLGIDADRIVVGGASAGGGLAATVAQRSLDEGCPLRGQALAYPMLDDRSVLRADHGGRGRFVWTPASNRFGWTAYLGHP
PDTSEAPAYAAAARRSELSGLAPAWIGVGQLDLLHDDCVSYAERLESSGVPCELITVPGMYHAADAIAARALSMQTFCAS
MLNFMRARLS

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=326, Percent_Identity=27.3006134969325, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI206597554, Length=162, Percent_Identity=32.7160493827161, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI157041239, Length=148, Percent_Identity=33.1081081081081, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21328446, Length=101, Percent_Identity=38.6138613861386, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=239, Percent_Identity=28.4518828451883, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI72001146, Length=276, Percent_Identity=28.2608695652174, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=131, Percent_Identity=38.9312977099237, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17540028, Length=142, Percent_Identity=33.8028169014084, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=129, Percent_Identity=34.8837209302326, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34641; Mature: 34641

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSAAAVIGGATAAVAAGRYLLAREALSAVARDLRSPVLPYVAAPSSRRSMLVTRAFTR
CCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHH
LPTPAGDGVTVTESRVGETAVRVVITAPAGGGTSRPAVMWIHSGGFVVGSPQLERFITGQ
CCCCCCCCEEEEHHHCCCEEEEEEEEECCCCCCCCCEEEEEECCCEEEECHHHHHHHHHH
IARELGATVVSPDYRLAPEHAVPAALDDCMATLRWMRTNADRLGIDADRIVVGGASAGGG
HHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCCC
LAATVAQRSLDEGCPLRGQALAYPMLDDRSVLRADHGGRGRFVWTPASNRFGWTAYLGHP
HHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHCCCCCCCCCEEECCCCCCCCEEEEECCC
PDTSEAPAYAAAARRSELSGLAPAWIGVGQLDLLHDDCVSYAERLESSGVPCELITVPGM
CCCCCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
YHAADAIAARALSMQTFCASMLNFMRARLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRSAAAVIGGATAAVAAGRYLLAREALSAVARDLRSPVLPYVAAPSSRRSMLVTRAFTR
CCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHH
LPTPAGDGVTVTESRVGETAVRVVITAPAGGGTSRPAVMWIHSGGFVVGSPQLERFITGQ
CCCCCCCCEEEEHHHCCCEEEEEEEEECCCCCCCCCEEEEEECCCEEEECHHHHHHHHHH
IARELGATVVSPDYRLAPEHAVPAALDDCMATLRWMRTNADRLGIDADRIVVGGASAGGG
HHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCCC
LAATVAQRSLDEGCPLRGQALAYPMLDDRSVLRADHGGRGRFVWTPASNRFGWTAYLGHP
HHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHCCCCCCCCCEEECCCCCCCCEEEEECCC
PDTSEAPAYAAAARRSELSGLAPAWIGVGQLDLLHDDCVSYAERLESSGVPCELITVPGM
CCCCCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
YHAADAIAARALSMQTFCASMLNFMRARLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]