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The map label for this gene is trpB [H]

Identifier: 108800016

GI number: 108800016

Start: 3227545

End: 3228807

Strand: Reverse

Name: trpB [H]

Synonym: Mmcs_3050

Alternate gene names: 108800016

Gene position: 3228807-3227545 (Counterclockwise)

Preceding gene: 108800017

Following gene: 108800015

Centisome position: 56.59

GC content: 68.65

Gene sequence:

>1263_bases
GTGGCCGAACTCGTCGACTCGGACCTGCCGCGCTCCAGTGCGGCCGTCGCCGAACCCACCAGCCACGACCCGGACGCCCG
CGGCCACTTCGGCCCGTACGGCGGACGCCTCGTTCCCGAGGCGCTGATGGCGGTCATCGAGGAGGTCACCGCGGCCTACG
AGAAGGCCCGTGGCGATCAGACGTTCCTCGACGAACTCGATCGGCTGCAGCGCCACTACAGCGGCCGTCCTTCGCCGCTG
TACGAGGCCACCCGGCTCAGCGAGCACGCCGGCGGGACGCGCATCTTCCTCAAACGAGAAGACCTCAACCACACGGGTTC
CCACAAGATCAACAATGTGCTCGGGCAGGCGCTGCTCGCACGTCAGATGGGCAAGACCCGGGTCATCGCCGAGACCGGCG
CCGGCCAGCACGGTGTCGCGACGGCCACCGCCTGCGCACTGCTCGGCCTGGACTGCGTGATCTACATGGGCGCGGTCGAC
ACGGCCCGTCAGGCGCTGAATGTGGCGCGCATGCGCCTGCTCGGCGCCGAGGTGGTCTCCGTGGAGGCCGGTTCGAAGAC
GCTCAAGGACGCGATCAACGAGGCCTTTCGCGACTGGGTCACCAACGCCGACGAGACGTACTACTGCTTCGGCACCGCGG
CGGGCCCGCACCCGTTCCCGTTGATGGTGCGCGATTTCCAGCGCATCATCGGCCTCGAAGCGCGGGTGCAGATCCAGGCC
CAGGCCGGCCGGCTCCCGGATGCGGTGACGGCGTGCGTCGGCGGCGGCTCCAATGCGATCGGCGTCTTCCACGCCTTCAT
CGACGATCCCCGTGTGCGGTTGATCGGTTACGAGGCGGCCGGAGACGGGGTCGAAACGGGCCGGCACGCCGCCACGTTCA
CCGGCGGCTCACCCGGTGCGTTCCAGGGCTCCTTCTCCTACCTGCTGCAGGACGAGGACGGCCAGACGATCGAATCGCAT
TCGATCTCGGCCGGTCTGGACTATCCAGGCGTGGGGCCCGAGCACGCGCTGCTCAAGGACATCGGTCGCGCCGAGTACCG
GCCCATCACCGATACCGAGGCCATGGACGCGTTCTCGCTGCTGTGCCGCACCGAGGGGATCATCCCGGCCATCGAGTCCG
CGCACGCGGTCGCCGGTGCGCTCAAGCTCGGCCCGGAACTCGGGCCGGGTTCGGTGATCCTGGTCAACCTGTCCGGGCGC
GGTGACAAGGACGTCGAGACCGCAGCGAAATGGTTCGGTCTGCTGGACAAAGGCGGTTCATGA

Upstream 100 bases:

>100_bases
GTCGGCGAAGGCCTGGTGACCAGCCGCGACCCCCGCAGTGCGGTGGCCGACCTGGTGACCGCCGGAACCCATCCGTCCTG
CCCGAAACCGTCCCGATAGC

Downstream 100 bases:

>100_bases
GTCGGTTGGGTGGCTTGTTCGACGCTTGCCGAGCGGAGCGACGCGCGGCGCTCATCGGATACCTGCCAACCGGATTCCCC
GACGTGCCGGCATCGATCTC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MAELVDSDLPRSSAAVAEPTSHDPDARGHFGPYGGRLVPEALMAVIEEVTAAYEKARGDQTFLDELDRLQRHYSGRPSPL
YEATRLSEHAGGTRIFLKREDLNHTGSHKINNVLGQALLARQMGKTRVIAETGAGQHGVATATACALLGLDCVIYMGAVD
TARQALNVARMRLLGAEVVSVEAGSKTLKDAINEAFRDWVTNADETYYCFGTAAGPHPFPLMVRDFQRIIGLEARVQIQA
QAGRLPDAVTACVGGGSNAIGVFHAFIDDPRVRLIGYEAAGDGVETGRHAATFTGGSPGAFQGSFSYLLQDEDGQTIESH
SISAGLDYPGVGPEHALLKDIGRAEYRPITDTEAMDAFSLLCRTEGIIPAIESAHAVAGALKLGPELGPGSVILVNLSGR
GDKDVETAAKWFGLLDKGGS

Sequences:

>Translated_420_residues
MAELVDSDLPRSSAAVAEPTSHDPDARGHFGPYGGRLVPEALMAVIEEVTAAYEKARGDQTFLDELDRLQRHYSGRPSPL
YEATRLSEHAGGTRIFLKREDLNHTGSHKINNVLGQALLARQMGKTRVIAETGAGQHGVATATACALLGLDCVIYMGAVD
TARQALNVARMRLLGAEVVSVEAGSKTLKDAINEAFRDWVTNADETYYCFGTAAGPHPFPLMVRDFQRIIGLEARVQIQA
QAGRLPDAVTACVGGGSNAIGVFHAFIDDPRVRLIGYEAAGDGVETGRHAATFTGGSPGAFQGSFSYLLQDEDGQTIESH
SISAGLDYPGVGPEHALLKDIGRAEYRPITDTEAMDAFSLLCRTEGIIPAIESAHAVAGALKLGPELGPGSVILVNLSGR
GDKDVETAAKWFGLLDKGGS
>Mature_419_residues
AELVDSDLPRSSAAVAEPTSHDPDARGHFGPYGGRLVPEALMAVIEEVTAAYEKARGDQTFLDELDRLQRHYSGRPSPLY
EATRLSEHAGGTRIFLKREDLNHTGSHKINNVLGQALLARQMGKTRVIAETGAGQHGVATATACALLGLDCVIYMGAVDT
ARQALNVARMRLLGAEVVSVEAGSKTLKDAINEAFRDWVTNADETYYCFGTAAGPHPFPLMVRDFQRIIGLEARVQIQAQ
AGRLPDAVTACVGGGSNAIGVFHAFIDDPRVRLIGYEAAGDGVETGRHAATFTGGSPGAFQGSFSYLLQDEDGQTIESHS
ISAGLDYPGVGPEHALLKDIGRAEYRPITDTEAMDAFSLLCRTEGIIPAIESAHAVAGALKLGPELGPGSVILVNLSGRG
DKDVETAAKWFGLLDKGGS

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=55.3805774278215, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321412, Length=382, Percent_Identity=57.3298429319372, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 44508; Mature: 44376

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELVDSDLPRSSAAVAEPTSHDPDARGHFGPYGGRLVPEALMAVIEEVTAAYEKARGDQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
TFLDELDRLQRHYSGRPSPLYEATRLSEHAGGTRIFLKREDLNHTGSHKINNVLGQALLA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
RQMGKTRVIAETGAGQHGVATATACALLGLDCVIYMGAVDTARQALNVARMRLLGAEVVS
HHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEE
VEAGSKTLKDAINEAFRDWVTNADETYYCFGTAAGPHPFPLMVRDFQRIIGLEARVQIQA
ECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEE
QAGRLPDAVTACVGGGSNAIGVFHAFIDDPRVRLIGYEAAGDGVETGRHAATFTGGSPGA
CCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCC
FQGSFSYLLQDEDGQTIESHSISAGLDYPGVGPEHALLKDIGRAEYRPITDTEAMDAFSL
CCCCEEEEEECCCCCEEHHCCCCCCCCCCCCCCHHHHHHHHCHHCCCCCCCHHHHHHHHH
LCRTEGIIPAIESAHAVAGALKLGPELGPGSVILVNLSGRGDKDVETAAKWFGLLDKGGS
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AELVDSDLPRSSAAVAEPTSHDPDARGHFGPYGGRLVPEALMAVIEEVTAAYEKARGDQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
TFLDELDRLQRHYSGRPSPLYEATRLSEHAGGTRIFLKREDLNHTGSHKINNVLGQALLA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
RQMGKTRVIAETGAGQHGVATATACALLGLDCVIYMGAVDTARQALNVARMRLLGAEVVS
HHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEE
VEAGSKTLKDAINEAFRDWVTNADETYYCFGTAAGPHPFPLMVRDFQRIIGLEARVQIQA
ECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEE
QAGRLPDAVTACVGGGSNAIGVFHAFIDDPRVRLIGYEAAGDGVETGRHAATFTGGSPGA
CCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCC
FQGSFSYLLQDEDGQTIESHSISAGLDYPGVGPEHALLKDIGRAEYRPITDTEAMDAFSL
CCCCEEEEEECCCCCEEHHCCCCCCCCCCCCCCHHHHHHHHCHHCCCCCCCHHHHHHHHH
LCRTEGIIPAIESAHAVAGALKLGPELGPGSVILVNLSGRGDKDVETAAKWFGLLDKGGS
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]