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The map label for this gene is ysgA [H]

Identifier: 108799944

GI number: 108799944

Start: 3144975

End: 3145721

Strand: Reverse

Name: ysgA [H]

Synonym: Mmcs_2978

Alternate gene names: 108799944

Gene position: 3145721-3144975 (Counterclockwise)

Preceding gene: 108799945

Following gene: 108799941

Centisome position: 55.14

GC content: 72.56

Gene sequence:

>747_bases
GTGGCGGCAGCGGTCAAACTCCACCGACACGTCGGTCGCCGCCGCGCCGCACGTTTCCTCGCCGAGGGGCCCAACCTCGT
CGAGGCCGCGTTGCGGCGCGGTGTCGTCTCCGAGGTCTTCGCCACCGAGGACGCCGCGGCGCGGTTCGCGGCGCTGCTCG
CCGGCGCCGATGTCCACCTCGTCACCGAGCGCGCGGCGAAAGCACTGTCGGACACGGTGACTCCGGTCGGCCTCGTCGCC
GTGTGTCGGATGCCCGAGGTGTCGCTCGACGAAGTGCTCGGTGCGTCCCCACGACTCGTGGCGGTCGCGGTCGAGACGTC
CGAACCCGGGAATGCCGGCACTCTGATCCGGCTCGCGGATGCGATGGGCGCCGACGCGGTGATCCTGGCAGGCAACAGCG
TGGACCCCTACAACGGGAAGTGTCTGCGTGCCTCGGCGGGCAGCATCTTCGGGGTGTCCGTCGTCGAGGCACCCGACACC
GCCGGTCTGGTCGCCGCGCTGCACGGCGCCGGGCTGACTGTCCTGGCCACCATGCTCGACGGTGAGGTGAGCCTCGACGA
TGCGGAGCTGTCCGGCCCGACGGCGTGGCTGTTCGGCGCCGAGGCCCACGGACTCGCCCCAGATGTCGCCGCGCTCGCCG
ATGCCCGGGTCACCATCCCGATGGCGGGCAGCGCCGAGAGCCTCAACGTCGCCGCCGCCGCCGCGATCTGCCTGTACCAG
AGCGCCCGCGCGCTGCGCTCGAGCTGA

Upstream 100 bases:

>100_bases
TCACCGCACTGGTGGAGGTCGCCAAGGCCGCGCTGCCCGAGGACGTCAATGCGCCGTCCGGAGAGGCCGCCTGAGCCTGC
TCAGTGAGCGCTCCGCCCGG

Downstream 100 bases:

>100_bases
CTTCGTCGGTCGCGCCGCGAGACTGAACCCACGCGGGCCCCCACCCGCACTCTTGCTGCGTGGTCTCAGTCTCGCGGAAC
TGCGCGCGAGCCGCTACCCG

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MAAAVKLHRHVGRRRAARFLAEGPNLVEAALRRGVVSEVFATEDAAARFAALLAGADVHLVTERAAKALSDTVTPVGLVA
VCRMPEVSLDEVLGASPRLVAVAVETSEPGNAGTLIRLADAMGADAVILAGNSVDPYNGKCLRASAGSIFGVSVVEAPDT
AGLVAALHGAGLTVLATMLDGEVSLDDAELSGPTAWLFGAEAHGLAPDVAALADARVTIPMAGSAESLNVAAAAAICLYQ
SARALRSS

Sequences:

>Translated_248_residues
MAAAVKLHRHVGRRRAARFLAEGPNLVEAALRRGVVSEVFATEDAAARFAALLAGADVHLVTERAAKALSDTVTPVGLVA
VCRMPEVSLDEVLGASPRLVAVAVETSEPGNAGTLIRLADAMGADAVILAGNSVDPYNGKCLRASAGSIFGVSVVEAPDT
AGLVAALHGAGLTVLATMLDGEVSLDDAELSGPTAWLFGAEAHGLAPDVAALADARVTIPMAGSAESLNVAAAAAICLYQ
SARALRSS
>Mature_247_residues
AAAVKLHRHVGRRRAARFLAEGPNLVEAALRRGVVSEVFATEDAAARFAALLAGADVHLVTERAAKALSDTVTPVGLVAV
CRMPEVSLDEVLGASPRLVAVAVETSEPGNAGTLIRLADAMGADAVILAGNSVDPYNGKCLRASAGSIFGVSVVEAPDTA
GLVAALHGAGLTVLATMLDGEVSLDDAELSGPTAWLFGAEAHGLAPDVAALADARVTIPMAGSAESLNVAAAAAICLYQS
ARALRSS

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=158, Percent_Identity=34.8101265822785, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790623, Length=137, Percent_Identity=29.1970802919708, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24666840, Length=266, Percent_Identity=25.9398496240602, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 25011; Mature: 24880

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAVKLHRHVGRRRAARFLAEGPNLVEAALRRGVVSEVFATEDAAARFAALLAGADVHL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEE
VTERAAKALSDTVTPVGLVAVCRMPEVSLDEVLGASPRLVAVAVETSEPGNAGTLIRLAD
HHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEC
AMGADAVILAGNSVDPYNGKCLRASAGSIFGVSVVEAPDTAGLVAALHGAGLTVLATMLD
CCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHC
GEVSLDDAELSGPTAWLFGAEAHGLAPDVAALADARVTIPMAGSAESLNVAAAAAICLYQ
CCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
SARALRSS
HHHHHHCC
>Mature Secondary Structure 
AAAVKLHRHVGRRRAARFLAEGPNLVEAALRRGVVSEVFATEDAAARFAALLAGADVHL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEE
VTERAAKALSDTVTPVGLVAVCRMPEVSLDEVLGASPRLVAVAVETSEPGNAGTLIRLAD
HHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEC
AMGADAVILAGNSVDPYNGKCLRASAGSIFGVSVVEAPDTAGLVAALHGAGLTVLATMLD
CCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHC
GEVSLDDAELSGPTAWLFGAEAHGLAPDVAALADARVTIPMAGSAESLNVAAAAAICLYQ
CCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
SARALRSS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]