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The map label for this gene is fadD26 [H]

Identifier: 108799802

GI number: 108799802

Start: 2999716

End: 3001458

Strand: Reverse

Name: fadD26 [H]

Synonym: Mmcs_2836

Alternate gene names: 108799802

Gene position: 3001458-2999716 (Counterclockwise)

Preceding gene: 108799806

Following gene: 108799801

Centisome position: 52.61

GC content: 67.7

Gene sequence:

>1743_bases
ATGACTGAATCGTCTGTGACAGCCCTGCTGCAGGAGCGGGCGCTGCACCAGCCCGACGAGCCCGCGTACACGTTCATCGA
CTACGACGTCGACCCGAAGGGGTACGCCGAGACCCTCACCTGGGCTCAGCTGCACAACCGGGCACAGGTGCTGGCCGAGG
AGCTGATGCTGTTCGCCGGCGCCGGTGACCGCGCCGCGATCCTCGCTCCCCAGGGCCTGGACTACATCGTCGCCTTCTTC
GGCGCGCTGCAGGCGGGACTCATCGCCGCTCCACTCTCGGTCCCGGCGTACGGCGTGCACGACGAGCGGATCTCGAGCGT
GCTGCGGGACTGCGCACCGTCGGTCATCCTGACGACATCGAGCGTCGCGGGTGAAGTCGGCCGGTATGCACTGGGCGGAG
GCGGCCGAGCGGCAACGGTGGTCGAGGTCGACTCGCTGGATCTCGACGCCCCCGCACAGTCTGCTGCGCCCCCGGCCCAG
AAGTCCAAGATCGCGTACTTGCAGTACACCTCGGGGTCGACGCGGGTGCCGGCCGGTGTGGTCGTCACCCACGACAACGT
GGTGGTCAACGTCGACCAGGCGTTCACCGACTACTTCGGTCACTCGGGGGCGACGCCTCCGGGCATGACCTTCGTATCGT
GGCTGCCCTTCTACCACGACATGGGTCTGATCAAGGGTGTGTGCGCGCCGTTGGTGAGCGGACGTTCCGCGGTCCTGATG
AGCCCCATGGCATTCCTGCAGCGCCCCGCCCGCTGGATCCAGCAGATCGCGATCAATCCCGGATCATTCACGGCGGCACC
GAATTTCGCGTTCGAGTTGGCGGTCCGGCGGACCACCGACGAGGACATGGCCGGGCTCGATCTCGGCGCGGTGCTCGGAA
TCTGCAGCGGAAGTGAACGGGTCCACGCGGCGACGGTCAACCGTTTCGTCGAACGCTTCAGCCGCTTCGGCCTGAATGCG
GCGTCGGTCAAACCCTCGTACGGGCTTGCCGAGGCCACCGTCTATGTGGCGACGTCCGACCGAGGCCACGCACCGAACAT
CGTCCGGTTCGACTACGAGAAGCTGTCGAGCGGGCACGCGATGCGCTGTGAGAGCGAAGCCGCCGGTGGCACGGATCTGC
TCGGTCACGGGACGGCCCGGTCGACCACACTGCGCATCGTCGATCCGGAGAGCGGCACCGAGAACCCCGCCGGCCAGGTC
GGGGAGATCTGGGTCCATGGGGCGAACGTCGCCCGGGGTTACTGGCAGCGCCCGAAGGAGACCGAGCGGGTGTTCTCAGG
GCGCCTGGTCAAGCCGTCGGCGGGGACCGCATCGGGACCGTGGCTGCGCACCGGCGACCTCGGCGTCATCTCCGACGGTG
AACTGTTCATCATCGGCCGGATCAAGGACCTCCTGATCGTGGACGGGTCGAACCACTACCCGGACGACATCGAGGCCACG
ATCCAGGAACTCTCCGGCGGTCGGGTGGCCGCGATCTCGGTGCCCAGCGGGGAGACCGAACATCTCGTCACCATCGTCGA
GGTCAAGAAGCGCGGCTCCGACGACGAGCACCGCGAACGGCTGCGCACCCTCAAACGCCGGATCACCGCGGCTGTTTCGA
CCACCCACCGGGTTCGGGCCGCCGACGTCGTGTTCGTCGCGCCCGGCGCCATCCCGATCACCACGAGCGGCAAGATCCGG
CGTTCCACCTGTGTGGAGCGCTACGGGCGCGGGGAGTTCGAGCGTCTGGATCAGTCGGCATGA

Upstream 100 bases:

>100_bases
GGGGAGGACGCGCCGCGACGCCGGGCCGCTTGCAGCGTCGTCGCGTGACGAGCACATACGGACTTCTCAACGGACGGCCA
CTGAAGAAACGAGTAGGACC

Downstream 100 bases:

>100_bases
CACCGGCCACGCTCGACGACGCGAGGCTGCGCGACTGGTTGGTCACCTATCTGACCACGCACGTCGAGTGCTCACCCGAG
AGCATCGACTTCGACGCGTC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MTESSVTALLQERALHQPDEPAYTFIDYDVDPKGYAETLTWAQLHNRAQVLAEELMLFAGAGDRAAILAPQGLDYIVAFF
GALQAGLIAAPLSVPAYGVHDERISSVLRDCAPSVILTTSSVAGEVGRYALGGGGRAATVVEVDSLDLDAPAQSAAPPAQ
KSKIAYLQYTSGSTRVPAGVVVTHDNVVVNVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLM
SPMAFLQRPARWIQQIAINPGSFTAAPNFAFELAVRRTTDEDMAGLDLGAVLGICSGSERVHAATVNRFVERFSRFGLNA
ASVKPSYGLAEATVYVATSDRGHAPNIVRFDYEKLSSGHAMRCESEAAGGTDLLGHGTARSTTLRIVDPESGTENPAGQV
GEIWVHGANVARGYWQRPKETERVFSGRLVKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEAT
IQELSGGRVAAISVPSGETEHLVTIVEVKKRGSDDEHRERLRTLKRRITAAVSTTHRVRAADVVFVAPGAIPITTSGKIR
RSTCVERYGRGEFERLDQSA

Sequences:

>Translated_580_residues
MTESSVTALLQERALHQPDEPAYTFIDYDVDPKGYAETLTWAQLHNRAQVLAEELMLFAGAGDRAAILAPQGLDYIVAFF
GALQAGLIAAPLSVPAYGVHDERISSVLRDCAPSVILTTSSVAGEVGRYALGGGGRAATVVEVDSLDLDAPAQSAAPPAQ
KSKIAYLQYTSGSTRVPAGVVVTHDNVVVNVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLM
SPMAFLQRPARWIQQIAINPGSFTAAPNFAFELAVRRTTDEDMAGLDLGAVLGICSGSERVHAATVNRFVERFSRFGLNA
ASVKPSYGLAEATVYVATSDRGHAPNIVRFDYEKLSSGHAMRCESEAAGGTDLLGHGTARSTTLRIVDPESGTENPAGQV
GEIWVHGANVARGYWQRPKETERVFSGRLVKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEAT
IQELSGGRVAAISVPSGETEHLVTIVEVKKRGSDDEHRERLRTLKRRITAAVSTTHRVRAADVVFVAPGAIPITTSGKIR
RSTCVERYGRGEFERLDQSA
>Mature_579_residues
TESSVTALLQERALHQPDEPAYTFIDYDVDPKGYAETLTWAQLHNRAQVLAEELMLFAGAGDRAAILAPQGLDYIVAFFG
ALQAGLIAAPLSVPAYGVHDERISSVLRDCAPSVILTTSSVAGEVGRYALGGGGRAATVVEVDSLDLDAPAQSAAPPAQK
SKIAYLQYTSGSTRVPAGVVVTHDNVVVNVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLMS
PMAFLQRPARWIQQIAINPGSFTAAPNFAFELAVRRTTDEDMAGLDLGAVLGICSGSERVHAATVNRFVERFSRFGLNAA
SVKPSYGLAEATVYVATSDRGHAPNIVRFDYEKLSSGHAMRCESEAAGGTDLLGHGTARSTTLRIVDPESGTENPAGQVG
EIWVHGANVARGYWQRPKETERVFSGRLVKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEATI
QELSGGRVAAISVPSGETEHLVTIVEVKKRGSDDEHRERLRTLKRRITAAVSTTHRVRAADVVFVAPGAIPITTSGKIRR
STCVERYGRGEFERLDQSA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=590, Percent_Identity=25.4237288135593, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI225735629, Length=617, Percent_Identity=23.9870340356564, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI45827692, Length=617, Percent_Identity=23.9870340356564, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI225735625, Length=617, Percent_Identity=23.9870340356564, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI55749758, Length=575, Percent_Identity=25.2173913043478, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI45827694, Length=559, Percent_Identity=24.5080500894454, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI225735627, Length=517, Percent_Identity=24.9516441005803, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI45827696, Length=517, Percent_Identity=24.7582205029014, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI45827698, Length=352, Percent_Identity=25.2840909090909, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788107, Length=597, Percent_Identity=25.963149078727, Blast_Score=138, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI193202679, Length=602, Percent_Identity=23.0897009966777, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=561, Percent_Identity=24.0641711229947, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=409, Percent_Identity=22.2493887530562, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24654656, Length=621, Percent_Identity=23.6714975845411, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24581924, Length=554, Percent_Identity=25.0902527075812, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281365686, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653035, Length=184, Percent_Identity=30.9782608695652, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161076582, Length=175, Percent_Identity=30.2857142857143, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62044; Mature: 61912

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESSVTALLQERALHQPDEPAYTFIDYDVDPKGYAETLTWAQLHNRAQVLAEELMLFAG
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEC
AGDRAAILAPQGLDYIVAFFGALQAGLIAAPLSVPAYGVHDERISSVLRDCAPSVILTTS
CCCCEEEECCCCHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
SVAGEVGRYALGGGGRAATVVEVDSLDLDAPAQSAAPPAQKSKIAYLQYTSGSTRVPAGV
HHHHHHCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHCEEEEEEECCCCCCCCCEE
VVTHDNVVVNVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLM
EEECCCEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SPMAFLQRPARWIQQIAINPGSFTAAPNFAFELAVRRTTDEDMAGLDLGAVLGICSGSER
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHCCCCHHHHHHHCCCCCC
VHAATVNRFVERFSRFGLNAASVKPSYGLAEATVYVATSDRGHAPNIVRFDYEKLSSGHA
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECHHHHCCCCC
MRCESEAAGGTDLLGHGTARSTTLRIVDPESGTENPAGQVGEIWVHGANVARGYWQRPKE
EEECCCCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHCCCHH
TERVFSGRLVKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEAT
HHHHHCCEEECCCCCCCCCCEEECCCEEEECCCCEEEEEEEEEEEEECCCCCCCCHHHHH
IQELSGGRVAAISVPSGETEHLVTIVEVKKRGSDDEHRERLRTLKRRITAAVSTTHRVRA
HHHCCCCEEEEEECCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEE
ADVVFVAPGAIPITTSGKIRRSTCVERYGRGEFERLDQSA
EEEEEECCCCEEECCCCCCHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
TESSVTALLQERALHQPDEPAYTFIDYDVDPKGYAETLTWAQLHNRAQVLAEELMLFAG
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEC
AGDRAAILAPQGLDYIVAFFGALQAGLIAAPLSVPAYGVHDERISSVLRDCAPSVILTTS
CCCCEEEECCCCHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
SVAGEVGRYALGGGGRAATVVEVDSLDLDAPAQSAAPPAQKSKIAYLQYTSGSTRVPAGV
HHHHHHCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHCEEEEEEECCCCCCCCCEE
VVTHDNVVVNVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLM
EEECCCEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SPMAFLQRPARWIQQIAINPGSFTAAPNFAFELAVRRTTDEDMAGLDLGAVLGICSGSER
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHCCCCHHHHHHHCCCCCC
VHAATVNRFVERFSRFGLNAASVKPSYGLAEATVYVATSDRGHAPNIVRFDYEKLSSGHA
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECHHHHCCCCC
MRCESEAAGGTDLLGHGTARSTTLRIVDPESGTENPAGQVGEIWVHGANVARGYWQRPKE
EEECCCCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHCCCHH
TERVFSGRLVKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEAT
HHHHHCCEEECCCCCCCCCCEEECCCEEEECCCCEEEEEEEEEEEEECCCCCCCCHHHHH
IQELSGGRVAAISVPSGETEHLVTIVEVKKRGSDDEHRERLRTLKRRITAAVSTTHRVRA
HHHCCCCEEEEEECCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEE
ADVVFVAPGAIPITTSGKIRRSTCVERYGRGEFERLDQSA
EEEEEECCCCEEECCCCCCHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]