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The map label for this gene is gph [C]

Identifier: 108799712

GI number: 108799712

Start: 2891302

End: 2892024

Strand: Reverse

Name: gph [C]

Synonym: Mmcs_2746

Alternate gene names: 108799712

Gene position: 2892024-2891302 (Counterclockwise)

Preceding gene: 108799713

Following gene: 108799709

Centisome position: 50.69

GC content: 66.53

Gene sequence:

>723_bases
ATGACCAACTCGCACAGCACGGCCGGTTCCGCACCGGCCGTGCTGTTCGATATCGACGGCACCCTGGTCGACTCGAACTA
TCTGCATGTCCACGCTTGGCACCGGTCTTTCGCCGACGAGGGGCTGACGGTTGAATCCTGGCGGGTGCACCGGTGCATCG
GCATGGACGGCTCGACGTTGTTGGACAACCTGGTTCCCGATGCCGACGACGACACGCGAAAGCGTCTGAAGGACGGGCAT
TCTCGCTACTACCTCGACGACAGGGGCCTGTTACGCCTGCTTCCCGGTGCGCGGGCACTCCTCGAGCGGATCAAGGAGCT
CGGCCTACAGGTGGTGCTGGCCACCTCGGCGCCCGAGGACGAACTCGACGTGCTCCGTGAGGTGCTCGACAGTGAAGACG
TCTACTCCGCCATGACGTCCGGGGAGGACGTCGACACCGCCAAACCCCAGCCCGACATCGTCCACATCGCTCTGGAACGC
GCGGGTGTGGACGCCGAGCACGCGGTGTTCGTCGGCGACGCCGTATGGGACATCGAGGCCTGTCGGCGAGCCGGCCTGAC
ATCCATCGGCGTGCTCAGCGGTGGCGTCTCCCGCGGCGAGCTCACCGAGGCGGGTGCCCGCGCGGTCTTCGAGAACGCCG
AAGACCTGCGCGTGCACATCGACGAGAGCCCCGTCGCCGCGCTGCTGAGCCGTGCCGACACGAACAGTCAGGCCAAGGCG
TAA

Upstream 100 bases:

>100_bases
GGTACTCCCAGCAGCCCGGTGGCACCCAGCAGCCCGGCTACCCGCAGCAGCCCGGCGCCGGGCCGGACAACGGTCGCACC
GCGACCGACCGTCCCACCCG

Downstream 100 bases:

>100_bases
CGCAGCACCGCGGCGATTCCGTCGTCCGGGTCGATTCGCTCGTCGGTGCGGACCAGCGCCGCACCGGTGCGTACGGCGGC
AAGGGGAAGCGCCTCGTCGG

Product: HAD family hydrolase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTNSHSTAGSAPAVLFDIDGTLVDSNYLHVHAWHRSFADEGLTVESWRVHRCIGMDGSTLLDNLVPDADDDTRKRLKDGH
SRYYLDDRGLLRLLPGARALLERIKELGLQVVLATSAPEDELDVLREVLDSEDVYSAMTSGEDVDTAKPQPDIVHIALER
AGVDAEHAVFVGDAVWDIEACRRAGLTSIGVLSGGVSRGELTEAGARAVFENAEDLRVHIDESPVAALLSRADTNSQAKA

Sequences:

>Translated_240_residues
MTNSHSTAGSAPAVLFDIDGTLVDSNYLHVHAWHRSFADEGLTVESWRVHRCIGMDGSTLLDNLVPDADDDTRKRLKDGH
SRYYLDDRGLLRLLPGARALLERIKELGLQVVLATSAPEDELDVLREVLDSEDVYSAMTSGEDVDTAKPQPDIVHIALER
AGVDAEHAVFVGDAVWDIEACRRAGLTSIGVLSGGVSRGELTEAGARAVFENAEDLRVHIDESPVAALLSRADTNSQAKA
>Mature_239_residues
TNSHSTAGSAPAVLFDIDGTLVDSNYLHVHAWHRSFADEGLTVESWRVHRCIGMDGSTLLDNLVPDADDDTRKRLKDGHS
RYYLDDRGLLRLLPGARALLERIKELGLQVVLATSAPEDELDVLREVLDSEDVYSAMTSGEDVDTAKPQPDIVHIALERA
GVDAEHAVFVGDAVWDIEACRRAGLTSIGVLSGGVSRGELTEAGARAVFENAEDLRVHIDESPVAALLSRADTNSQAKA

Specific function: Displays high phosphatase activity toward erythrose 4- phosphate, fructose 6-phosphate, 2-deoxyglucose 6-phosphate, and mannose 6-phosphate. May have a role in the intracellular metabolism of many phosphorylated carbohydrates [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=211, Percent_Identity=30.8056872037915, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787576, Length=193, Percent_Identity=23.8341968911917, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 25979; Mature: 25848

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSHSTAGSAPAVLFDIDGTLVDSNYLHVHAWHRSFADEGLTVESWRVHRCIGMDGSTL
CCCCCCCCCCCCEEEEECCCEEECCCEEEEEHHHHHHHCCCCEECCEEEEEEECCCCHHH
LDNLVPDADDDTRKRLKDGHSRYYLDDRGLLRLLPGARALLERIKELGLQVVLATSAPED
HHHHCCCCCCHHHHHHHCCCCEEEECCCCHHHHCCCHHHHHHHHHHCCEEEEEECCCCCH
ELDVLREVLDSEDVYSAMTSGEDVDTAKPQPDIVHIALERAGVDAEHAVFVGDAVWDIEA
HHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHH
CRRAGLTSIGVLSGGVSRGELTEAGARAVFENAEDLRVHIDESPVAALLSRADTNSQAKA
HHHCCCCEEHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TNSHSTAGSAPAVLFDIDGTLVDSNYLHVHAWHRSFADEGLTVESWRVHRCIGMDGSTL
CCCCCCCCCCCEEEEECCCEEECCCEEEEEHHHHHHHCCCCEECCEEEEEEECCCCHHH
LDNLVPDADDDTRKRLKDGHSRYYLDDRGLLRLLPGARALLERIKELGLQVVLATSAPED
HHHHCCCCCCHHHHHHHCCCCEEEECCCCHHHHCCCHHHHHHHHHHCCEEEEEECCCCCH
ELDVLREVLDSEDVYSAMTSGEDVDTAKPQPDIVHIALERAGVDAEHAVFVGDAVWDIEA
HHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHH
CRRAGLTSIGVLSGGVSRGELTEAGARAVFENAEDLRVHIDESPVAALLSRADTNSQAKA
HHHCCCCEEHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]