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The map label for this gene is hypF [H]

Identifier: 108799103

GI number: 108799103

Start: 2279583

End: 2281820

Strand: Reverse

Name: hypF [H]

Synonym: Mmcs_2136

Alternate gene names: 108799103

Gene position: 2281820-2279583 (Counterclockwise)

Preceding gene: 108799104

Following gene: 108799102

Centisome position: 39.99

GC content: 72.65

Gene sequence:

>2238_bases
GTGGTCCAGGGGGTCGGCTTCCGGCCCTTCGTGTACACCACCGCGACGGCGCTCGGACTGACCGGGTCGGTGCGCAACGA
CAGCGCGGGCGCGGTCGTCGAGATCGAGGGCGACCCGGACGCGCTGACCCGCTTCGTCGAGCGGTTGCACACCCGGCCGC
CACCGCTCGCGGTCATCGAATCCGTTGACATCGAAGACCTTCCGCCCGTCGGCGGCACCGGGTTCTTCATCGCCGACACC
TCTCGCGGCCGCGGCCGCACGCTGGCCTCACCGGATGTCGCGATGTGCGCGCAGTGCGCCGCCGAACAGCGCGACCCCGG
CGACCGGCGCTACCGGCACCCGTTCGTCAACTGCACCGACTGCGGACCCCGGTTCACCATCATCGCGTCGCTGCCCTACG
ACCGGGCCGCCACGTCGATGGCCGGTTTCGCGATGTGCGCGGACTGCGCCCGCGAGTACGCCGACCCCACCGACCGCCGC
TTCCACGCGCAACCCGTGTGCTGCCCGCACTGCGGCCCGACCCTGACCTTCCGCGACGCCGACGGACGCCACACTGCGGG
CGAGGACGGTCTGCGCCGGGCGCGGCGGCTGCTCCGCGACGGGGGTGTCGTCGCGGTGAAGGGCATCGGCGGCTACCACC
TGGCCTGCGATGCCGAGAACAACACCGCCGTGGCCGAACTACGCCGCCGGAAACAGCGCGGCGACAAGCCCTTCGCGGTC
ATGGTGCCCGATGCGGCCGCGGCCGGCCGGATCGCCGTGCTCGACGAGGTGGCACAGCACCTGCTGGGCGGGCCGCAGCG
GCCGATCGTGCTGGCGCCGCGACGGACACCGACCACGGTGGCGGCCGCGGTGGCGCCCGGGAACCCGGACCTGGGCGTGC
TGCTGGCCTATTCGCCGCTGCACACGCTGCTGTTCGGGCTGCCCGGTGACGAACCGGGCCCCCAGGTGCTGGTGATGACG
TCGGGGAACCTCGCGGGCGAACCCCTCTGCTACGACGACGACGACGCCGTCACCCGGCTTCGCGCACTGGCCGACGGCTG
GCTGATGCACGACCGCGAGATCCTGGTGCCCTGTGACGATTCGGTGGTCCGGGTGGTCGACGGCGTCGAACTGCCGATCC
GGCGCTCCCGGGGGTACGCCCCGTTGCCGGTGGCCCTGCCCCTCGCGGTACCGCCGACACTCGCGGTCGGTGCGGACCTG
AAGAACACCATGGCTGTCGCCGACGGCCGATACGCCTGGCTGAGCCAGCACATCGGCGACATGGACGACATCGCGACACT
GTCGGCGTTCACCGCCGCGGAACGGCATCTGCGCGAACTCACCGGCGTGGCCCCGCAGATCCTCGCGACCGACGCCCACC
CGCGGTACCGCTCGACCGGATGGGCCCAGCGACACGCCGGTGACCGGCCGGTGCGGGCGGTACAGCACCACCACGCCCAC
ATCGCCGCGGTGATGGCCGAGCACGGGCTCGACGGTTCGGCGCCCGTACTCGGATTCGCCTTCGACGGAACCGGATACGG
GCCCGACGGCGCGGTGTGGGGCGGGGAGGTCCTGCTCGCCGACTACAAGGGCTTCCGCAGGATCGCGGCGCTGGAGTACG
TGCCACTGGCCGGCGGCGATGTCAGCGTCCTGCGGCCGTACCGGATGGCGCTCGCACATCTGTGGGCCGCCGGGTTGCCG
TGGGCCGGCGACCTGCCGCCGGTGCAGGCGTGTCCGGAAGACGAGCGGCGGGTGCTGCGCCACCAACTGGAGACCGGCCT
GAACTGTGTGCCCACCTCCAGCATGGGGCGGTTGTTCGACTGCGTCGCCGCGCTCGCGGGGGTCCGGCAGACCGTCGCCT
ACGAGGCGCAGGCGGCGATCGAGCTCGAGGCGCTGGCGCGCACCGTCGACGACGATCCCGCGCCCTACGCCTTCACCGTC
ACCGAGTCCTCGGGGACGGCGATGATCGGCGCCGCCGCGGTGGTCGAGGCGATCGTCGCCGACCGCAGGCGCGGCACGGC
GGCCGCGGTGATCGGATGGCGATTCCACCATGCGGTGGCCGACCTCGTCGTACGGTTGGCCGAGGGAGCCCGCGACCCCG
TGGCGCTGTCGGGCGGGGTGTTCCAAAACGCCCTGCTCCTGCGCATGACGTTGCGCGCGTTGACCGAACGCGGTGTCGAG
GTGCTGACCCACCGCCGGGTGCCGCCGAACGACGGCGGCATCGCACTGGGTCAGTTGATGGTGGCCAACGCAGGGTGA

Upstream 100 bases:

>100_bases
ACGCGGCGATGGCCGGACTCGAACTCATGGGCCGCGGCGGACCGCCGGACCCGGAGGCCGCACCGTGACCCGCCGTCGGA
TGCGGTTGTGTGTGCGCGGC

Downstream 100 bases:

>100_bases
GGCCGGAGTAGAGGAGGACGCGGATGTGTTTGGCGGTACCGGGAAAGATCGACAGCGTCACCGACCGTGACGGCACGCTG
ATGGCCGTCGTCGACTTCGG

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MVQGVGFRPFVYTTATALGLTGSVRNDSAGAVVEIEGDPDALTRFVERLHTRPPPLAVIESVDIEDLPPVGGTGFFIADT
SRGRGRTLASPDVAMCAQCAAEQRDPGDRRYRHPFVNCTDCGPRFTIIASLPYDRAATSMAGFAMCADCAREYADPTDRR
FHAQPVCCPHCGPTLTFRDADGRHTAGEDGLRRARRLLRDGGVVAVKGIGGYHLACDAENNTAVAELRRRKQRGDKPFAV
MVPDAAAAGRIAVLDEVAQHLLGGPQRPIVLAPRRTPTTVAAAVAPGNPDLGVLLAYSPLHTLLFGLPGDEPGPQVLVMT
SGNLAGEPLCYDDDDAVTRLRALADGWLMHDREILVPCDDSVVRVVDGVELPIRRSRGYAPLPVALPLAVPPTLAVGADL
KNTMAVADGRYAWLSQHIGDMDDIATLSAFTAAERHLRELTGVAPQILATDAHPRYRSTGWAQRHAGDRPVRAVQHHHAH
IAAVMAEHGLDGSAPVLGFAFDGTGYGPDGAVWGGEVLLADYKGFRRIAALEYVPLAGGDVSVLRPYRMALAHLWAAGLP
WAGDLPPVQACPEDERRVLRHQLETGLNCVPTSSMGRLFDCVAALAGVRQTVAYEAQAAIELEALARTVDDDPAPYAFTV
TESSGTAMIGAAAVVEAIVADRRRGTAAAVIGWRFHHAVADLVVRLAEGARDPVALSGGVFQNALLLRMTLRALTERGVE
VLTHRRVPPNDGGIALGQLMVANAG

Sequences:

>Translated_745_residues
MVQGVGFRPFVYTTATALGLTGSVRNDSAGAVVEIEGDPDALTRFVERLHTRPPPLAVIESVDIEDLPPVGGTGFFIADT
SRGRGRTLASPDVAMCAQCAAEQRDPGDRRYRHPFVNCTDCGPRFTIIASLPYDRAATSMAGFAMCADCAREYADPTDRR
FHAQPVCCPHCGPTLTFRDADGRHTAGEDGLRRARRLLRDGGVVAVKGIGGYHLACDAENNTAVAELRRRKQRGDKPFAV
MVPDAAAAGRIAVLDEVAQHLLGGPQRPIVLAPRRTPTTVAAAVAPGNPDLGVLLAYSPLHTLLFGLPGDEPGPQVLVMT
SGNLAGEPLCYDDDDAVTRLRALADGWLMHDREILVPCDDSVVRVVDGVELPIRRSRGYAPLPVALPLAVPPTLAVGADL
KNTMAVADGRYAWLSQHIGDMDDIATLSAFTAAERHLRELTGVAPQILATDAHPRYRSTGWAQRHAGDRPVRAVQHHHAH
IAAVMAEHGLDGSAPVLGFAFDGTGYGPDGAVWGGEVLLADYKGFRRIAALEYVPLAGGDVSVLRPYRMALAHLWAAGLP
WAGDLPPVQACPEDERRVLRHQLETGLNCVPTSSMGRLFDCVAALAGVRQTVAYEAQAAIELEALARTVDDDPAPYAFTV
TESSGTAMIGAAAVVEAIVADRRRGTAAAVIGWRFHHAVADLVVRLAEGARDPVALSGGVFQNALLLRMTLRALTERGVE
VLTHRRVPPNDGGIALGQLMVANAG
>Mature_745_residues
MVQGVGFRPFVYTTATALGLTGSVRNDSAGAVVEIEGDPDALTRFVERLHTRPPPLAVIESVDIEDLPPVGGTGFFIADT
SRGRGRTLASPDVAMCAQCAAEQRDPGDRRYRHPFVNCTDCGPRFTIIASLPYDRAATSMAGFAMCADCAREYADPTDRR
FHAQPVCCPHCGPTLTFRDADGRHTAGEDGLRRARRLLRDGGVVAVKGIGGYHLACDAENNTAVAELRRRKQRGDKPFAV
MVPDAAAAGRIAVLDEVAQHLLGGPQRPIVLAPRRTPTTVAAAVAPGNPDLGVLLAYSPLHTLLFGLPGDEPGPQVLVMT
SGNLAGEPLCYDDDDAVTRLRALADGWLMHDREILVPCDDSVVRVVDGVELPIRRSRGYAPLPVALPLAVPPTLAVGADL
KNTMAVADGRYAWLSQHIGDMDDIATLSAFTAAERHLRELTGVAPQILATDAHPRYRSTGWAQRHAGDRPVRAVQHHHAH
IAAVMAEHGLDGSAPVLGFAFDGTGYGPDGAVWGGEVLLADYKGFRRIAALEYVPLAGGDVSVLRPYRMALAHLWAAGLP
WAGDLPPVQACPEDERRVLRHQLETGLNCVPTSSMGRLFDCVAALAGVRQTVAYEAQAAIELEALARTVDDDPAPYAFTV
TESSGTAMIGAAAVVEAIVADRRRGTAAAVIGWRFHHAVADLVVRLAEGARDPVALSGGVFQNALLLRMTLRALTERGVE
VLTHRRVPPNDGGIALGQLMVANAG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=760, Percent_Identity=40.5263157894737, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 79285; Mature: 79285

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQGVGFRPFVYTTATALGLTGSVRNDSAGAVVEIEGDPDALTRFVERLHTRPPPLAVIE
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEE
SVDIEDLPPVGGTGFFIADTSRGRGRTLASPDVAMCAQCAAEQRDPGDRRYRHPFVNCTD
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCEECC
CGPRFTIIASLPYDRAATSMAGFAMCADCAREYADPTDRRFHAQPVCCPHCGPTLTFRDA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECC
DGRHTAGEDGLRRARRLLRDGGVVAVKGIGGYHLACDAENNTAVAELRRRKQRGDKPFAV
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEE
MVPDAAAAGRIAVLDEVAQHLLGGPQRPIVLAPRRTPTTVAAAVAPGNPDLGVLLAYSPL
EECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCCCEEEEEEECHH
HTLLFGLPGDEPGPQVLVMTSGNLAGEPLCYDDDDAVTRLRALADGWLMHDREILVPCDD
HHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEECCC
SVVRVVDGVELPIRRSRGYAPLPVALPLAVPPTLAVGADLKNTMAVADGRYAWLSQHIGD
CHHHHHCCCCCCHHHCCCCCCCCEEECCCCCCCCEECCCCCHHEEECCCCHHHHHHHCCC
MDDIATLSAFTAAERHLRELTGVAPQILATDAHPRYRSTGWAQRHAGDRPVRAVQHHHAH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHHHHHH
IAAVMAEHGLDGSAPVLGFAFDGTGYGPDGAVWGGEVLLADYKGFRRIAALEYVPLAGGD
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHEEEECCCCC
VSVLRPYRMALAHLWAAGLPWAGDLPPVQACPEDERRVLRHQLETGLNCVPTSSMGRLFD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
CVAALAGVRQTVAYEAQAAIELEALARTVDDDPAPYAFTVTESSGTAMIGAAAVVEAIVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHH
DRRRGTAAAVIGWRFHHAVADLVVRLAEGARDPVALSGGVFQNALLLRMTLRALTERGVE
HCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCHH
VLTHRRVPPNDGGIALGQLMVANAG
HHHCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MVQGVGFRPFVYTTATALGLTGSVRNDSAGAVVEIEGDPDALTRFVERLHTRPPPLAVIE
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEE
SVDIEDLPPVGGTGFFIADTSRGRGRTLASPDVAMCAQCAAEQRDPGDRRYRHPFVNCTD
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCEECC
CGPRFTIIASLPYDRAATSMAGFAMCADCAREYADPTDRRFHAQPVCCPHCGPTLTFRDA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECC
DGRHTAGEDGLRRARRLLRDGGVVAVKGIGGYHLACDAENNTAVAELRRRKQRGDKPFAV
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEE
MVPDAAAAGRIAVLDEVAQHLLGGPQRPIVLAPRRTPTTVAAAVAPGNPDLGVLLAYSPL
EECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCCCEEEEEEECHH
HTLLFGLPGDEPGPQVLVMTSGNLAGEPLCYDDDDAVTRLRALADGWLMHDREILVPCDD
HHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEECCC
SVVRVVDGVELPIRRSRGYAPLPVALPLAVPPTLAVGADLKNTMAVADGRYAWLSQHIGD
CHHHHHCCCCCCHHHCCCCCCCCEEECCCCCCCCEECCCCCHHEEECCCCHHHHHHHCCC
MDDIATLSAFTAAERHLRELTGVAPQILATDAHPRYRSTGWAQRHAGDRPVRAVQHHHAH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHHHHHH
IAAVMAEHGLDGSAPVLGFAFDGTGYGPDGAVWGGEVLLADYKGFRRIAALEYVPLAGGD
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHEEEECCCCC
VSVLRPYRMALAHLWAAGLPWAGDLPPVQACPEDERRVLRHQLETGLNCVPTSSMGRLFD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
CVAALAGVRQTVAYEAQAAIELEALARTVDDDPAPYAFTVTESSGTAMIGAAAVVEAIVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHH
DRRRGTAAAVIGWRFHHAVADLVVRLAEGARDPVALSGGVFQNALLLRMTLRALTERGVE
HCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCHH
VLTHRRVPPNDGGIALGQLMVANAG
HHHCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]