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The map label for this gene is uvrD [H]

Identifier: 108798380

GI number: 108798380

Start: 1524956

End: 1527100

Strand: Direct

Name: uvrD [H]

Synonym: Mmcs_1409

Alternate gene names: 108798380

Gene position: 1524956-1527100 (Clockwise)

Preceding gene: 108798378

Following gene: 108798381

Centisome position: 26.73

GC content: 72.49

Gene sequence:

>2145_bases
TTGTCGGTCCGCCCTGCCAAGATGGGTCGCATGCCAGTCGAGGTCCCGACCTCGTCGCGGGAGCGGCTGCTTGCCGACCT
CGACGAGGAACAGCGTGAGGCCGTCCTGGCGCCTCGGGGGCCGGTCTGCGTGCTGGCCGGCGCCGGCACCGGCAAGACCC
GCACCATCACCCGCCGGATCGCCCACCTTGTCGCGGCGGGCCATGTCGCACCCGGGCAGGTGCTGGCCGTGACGTTCACC
TCCCGCGCCGCGGGGGAGATGCGGGCCCGGCTGCGGGCGCTCGACCAGGAGGCCGGCGGCGTCGGCACCGGGTCGGTACA
GGCGATGACGTTCCACGCCGCCGCCCGCCGCCAGCTGCGGTACTTCTGGCCCCGCGTGGTCGGCGACACCTCCTGGCAGC
TGCTCGACAGCAAGTTCTCCGTCGTGGCGCAGGCCGCCAACCGGGCTCGCGTGCAGGCCGCCACCGACGACGTCCGCGAC
CTGGCCGGTGAGATCGAGTGGGCGAAGGCGTCGCTGATCAGCCCGGAGGCCTACCCCGGCGCCGTCGCGCAGGCCGGCCG
CGACATCCCGATGGACGCCGCGAAGGTGGCCGCCGTCTACGCCGGTTACGAGGCGCTCAAGGCCCGCCACGACGGCACCG
CGCTGCTCGATTTCGACGATCTGCTGCTGCACACCGCGGCGGCGATCGAGAACGACGCGGCGGTGGCCGCGGAGTTCCGT
GACCGCTACCGCTGTTTCGTCGTCGACGAGTACCAGGACGTGACACCCCTGCAACAGCGCGTGCTCGACGCGTGGCTCGG
CCCCCGTGACGACCTGACCGTCGTCGGGGACGCCAACCAGACCATCTACTCGTTCACCGGGGCCACGCCGCGCTACCTGC
TGGACTTCTCCCGCCGGTTCCCCGACGCCGCGGTGATCCGGCTCGAACGCGACTACCGCTCCACCCCGCAGGTGGTGTCG
CTGGCCAACCGGGTGATCGCCGCCGCGCGCGGCCGCATGGCCGGCAGCAAGCTGCACCTGGTCGGCCAGCGCCCGGCGGG
GCCCACACCGGCGTTCGCCGAGCACACCGACGAGGTGGCCGAGGCCGCCGCCGTCGCCAAGGACATCCGCAGGCTGCTCG
ACGCCGGCACCCCCGCGGCGGAGATCGCGGTGCTGTACCGCATCAACGCGCAGTCGGAGGTCTACGAGGAGGCGCTCACC
GAGGCCGGCATCGCGTTCCAGGTGCGCGGTGGTGAGGGCTTCTTCAGCCGTCAGGAGATCCGCCAGGCGCTCGTGGCCCT
GCAGCGGGCCGCCGAACGCGACGTCGACGGTGAGGTGGCCGACGTCGTGCGGGCCGTGCTCGAACCGCTCGGGCTCACCG
CGGAGCCGCCGGCGGGCACCCGCGCCAGGGAACGCTGGGAGGCGCTCAGCGCGCTGGCCGAACTCGTCGACGAAGAGGTC
GTGCTGCGCCCGGGTCTTGACCTGCGCGGTCTGGTCACCGAGCTGCGCCAGCGCGCCGACGCCCGGCACCCACCGGTGGT
CCAGGGCGTCACGCTCGCGTCGCTGCATGCGGCGAAGGGGTTGGAGTGGGATGCGGTCTATCTCGTCGGGCTCGCCGACG
GCACCCTGCCGATCTCGCACGCGCTGACCCACGGCCCCGACAGTGAGCCGGTCGAGGAGGAGCGCCGGTTGCTCTATGTC
GGAATCACAAGGGCGCGAGTGCATCTCACATTGAGCTGGGCGCTGGCGCGGGCACCGGGGGGCCGCCAGGGTCGACGGCC
GTCGCGGTTCCTCAACGGTGTGGCGCCGCACTCGGCACCCGAGGCGTCGGTGAACAAACCGCGTCGCGCGCGGGGCGCGA
CGCCGCGCTGCCGGGTCTGCAACCAGGTGTTGACCACCCCCGCGGCAATCATGTTGCGCCGCTGCGAGACCTGCTCGTCC
GATATCGACGAACAACTGCTCGCCGCGCTCAAGGAGTGGCGGCTGCGGACCTCCAAGGAGCTCGGCGTCCCGGCCTTCGT
GGTGTTCACCGACAACACGCTGATCGCGATCGCCGAATCGCTGCCCACCGACGAGGCGGCGCTGGTCGCCATCCCCGGAA
TCGGCGCCCGCAAGCTCGAGCAGTACGGCGCCGACGTTCTCGCCTTGGTCAACGCACGCGGCTGA

Upstream 100 bases:

>100_bases
GCCGCACCACGTGGTGGTGTACATCGTCAGGGTGTCAGCAGCACTCATAAGGCATGCAACGTTACCGACCGCCCCGACAT
GCCCGACACGGGCCGGGCGG

Downstream 100 bases:

>100_bases
GACTGCCAACCACCGCAAAGATCGCGCCAAAAGCGCTGCTAGAAAATAGGTTGTGCGGTTCCGCTGCTAGGCTCTAGCCT
CAAGATCACACGTTGGGCGG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSVRPAKMGRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRIAHLVAAGHVAPGQVLAVTFT
SRAAGEMRARLRALDQEAGGVGTGSVQAMTFHAAARRQLRYFWPRVVGDTSWQLLDSKFSVVAQAANRARVQAATDDVRD
LAGEIEWAKASLISPEAYPGAVAQAGRDIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFR
DRYRCFVVDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRFPDAAVIRLERDYRSTPQVVS
LANRVIAAARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVAEAAAVAKDIRRLLDAGTPAAEIAVLYRINAQSEVYEEALT
EAGIAFQVRGGEGFFSRQEIRQALVALQRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEV
VLRPGLDLRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISHALTHGPDSEPVEEERRLLYV
GITRARVHLTLSWALARAPGGRQGRRPSRFLNGVAPHSAPEASVNKPRRARGATPRCRVCNQVLTTPAAIMLRRCETCSS
DIDEQLLAALKEWRLRTSKELGVPAFVVFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG

Sequences:

>Translated_714_residues
MSVRPAKMGRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRIAHLVAAGHVAPGQVLAVTFT
SRAAGEMRARLRALDQEAGGVGTGSVQAMTFHAAARRQLRYFWPRVVGDTSWQLLDSKFSVVAQAANRARVQAATDDVRD
LAGEIEWAKASLISPEAYPGAVAQAGRDIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFR
DRYRCFVVDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRFPDAAVIRLERDYRSTPQVVS
LANRVIAAARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVAEAAAVAKDIRRLLDAGTPAAEIAVLYRINAQSEVYEEALT
EAGIAFQVRGGEGFFSRQEIRQALVALQRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEV
VLRPGLDLRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISHALTHGPDSEPVEEERRLLYV
GITRARVHLTLSWALARAPGGRQGRRPSRFLNGVAPHSAPEASVNKPRRARGATPRCRVCNQVLTTPAAIMLRRCETCSS
DIDEQLLAALKEWRLRTSKELGVPAFVVFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG
>Mature_713_residues
SVRPAKMGRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRIAHLVAAGHVAPGQVLAVTFTS
RAAGEMRARLRALDQEAGGVGTGSVQAMTFHAAARRQLRYFWPRVVGDTSWQLLDSKFSVVAQAANRARVQAATDDVRDL
AGEIEWAKASLISPEAYPGAVAQAGRDIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFRD
RYRCFVVDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRFPDAAVIRLERDYRSTPQVVSL
ANRVIAAARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVAEAAAVAKDIRRLLDAGTPAAEIAVLYRINAQSEVYEEALTE
AGIAFQVRGGEGFFSRQEIRQALVALQRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEVV
LRPGLDLRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISHALTHGPDSEPVEEERRLLYVG
ITRARVHLTLSWALARAPGGRQGRRPSRFLNGVAPHSAPEASVNKPRRARGATPRCRVCNQVLTTPAAIMLRRCETCSSD
IDEQLLAALKEWRLRTSKELGVPAFVVFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=685, Percent_Identity=32.2627737226277, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI48994965, Length=444, Percent_Identity=30.4054054054054, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322369, Length=437, Percent_Identity=26.0869565217391, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324477, Length=395, Percent_Identity=21.2658227848101, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR002121
- InterPro:   IPR014016
- InterPro:   IPR010997 [H]

Pfam domain/function: PF00570 HRDC; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77152; Mature: 77020

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRPAKMGRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRI
CCCCCCHHCCCCEECCCCHHHHHHHHCCHHHHHEECCCCCCEEEEECCCCCCHHHHHHHH
AHLVAAGHVAPGQVLAVTFTSRAAGEMRARLRALDQEAGGVGTGSVQAMTFHAAARRQLR
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHH
YFWPRVVGDTSWQLLDSKFSVVAQAANRARVQAATDDVRDLAGEIEWAKASLISPEAYPG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
AVAQAGRDIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHH
DRYRCFVVDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRF
CCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHC
PDAAVIRLERDYRSTPQVVSLANRVIAAARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
EAAAVAKDIRRLLDAGTPAAEIAVLYRINAQSEVYEEALTEAGIAFQVRGGEGFFSRQEI
HHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
RQALVALQRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
VLRPGLDLRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISH
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHH
ALTHGPDSEPVEEERRLLYVGITRARVHLTLSWALARAPGGRQGRRPSRFLNGVAPHSAP
HHCCCCCCCCHHHCCCEEEEEEEEEEEEEEEEEHHHCCCCCCCCCCHHHHHCCCCCCCCC
EASVNKPRRARGATPRCRVCNQVLTTPAAIMLRRCETCSSDIDEQLLAALKEWRLRTSKE
CCCCCCCHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGVPAFVVFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG
CCCCEEEEEECCEEEEEECCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure 
SVRPAKMGRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRI
CCCCCHHCCCCEECCCCHHHHHHHHCCHHHHHEECCCCCCEEEEECCCCCCHHHHHHHH
AHLVAAGHVAPGQVLAVTFTSRAAGEMRARLRALDQEAGGVGTGSVQAMTFHAAARRQLR
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHH
YFWPRVVGDTSWQLLDSKFSVVAQAANRARVQAATDDVRDLAGEIEWAKASLISPEAYPG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
AVAQAGRDIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHH
DRYRCFVVDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRF
CCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHC
PDAAVIRLERDYRSTPQVVSLANRVIAAARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
EAAAVAKDIRRLLDAGTPAAEIAVLYRINAQSEVYEEALTEAGIAFQVRGGEGFFSRQEI
HHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
RQALVALQRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
VLRPGLDLRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISH
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHH
ALTHGPDSEPVEEERRLLYVGITRARVHLTLSWALARAPGGRQGRRPSRFLNGVAPHSAP
HHCCCCCCCCHHHCCCEEEEEEEEEEEEEEEEEHHHCCCCCCCCCCHHHHHCCCCCCCCC
EASVNKPRRARGATPRCRVCNQVLTTPAAIMLRRCETCSSDIDEQLLAALKEWRLRTSKE
CCCCCCCHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGVPAFVVFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG
CCCCEEEEEECCEEEEEECCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]