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The map label for this gene is sigF [H]

Identifier: 108798252

GI number: 108798252

Start: 1388668

End: 1389459

Strand: Direct

Name: sigF [H]

Synonym: Mmcs_1281

Alternate gene names: 108798252

Gene position: 1388668-1389459 (Clockwise)

Preceding gene: 108798251

Following gene: 108798254

Centisome position: 24.34

GC content: 67.55

Gene sequence:

>792_bases
GTGACGCCATCGTCGGCACAGGGTTCGTCGCCACGACAGAACTCTGAGTACTCGGACGTGGCCCCCATGTTCCGGGAACT
TCAGGGGCTCACCCCGAGCACACCTCAGTTCCAGCGTCAGCGCGACCGGATCGTCGAACGCTGCCTTCCGCTTGCCGATC
ACATCGCCCGTCGCTTCGACGGCCGCGGCGAGCCGCGCGACGACCTCGTCCAGGTCGCCCGGGTCGGCCTGGTCAACGCG
GTGATCCGCTTCGACGTCGACGCCGGATCGGATTTCGTGTCGTTCGCGGTGCCCACCATCATGGGTGAGGTCCGGCGGCA
CTTCCGGGACAACAGCTGGTCGGTCAAGGTGCCCCGCCGGCTCAAGGAACTGCACCTGCGCCTCGGGTCGGCGACCGCCG
AACTGTCCCAGCGCCTGGGCCGCGCGCCGACGGCCAGCGAACTCGCCGAGGAACTCGGCATGGACCGCGACGAGGTGGTC
GAGGGCCTGGTCGCAGGCAGTTCATACAACACGCTGTCGATCGACAGCGGCGGCAACAGCGGTAACGAGGATGCGCCCGC
GATCGCTGACACCCTCGGCGATGTGGACCTGGGTCTCGACCAGATCGAGAACCGCGAGGCACTGCGACCGCTGCTGGCCG
CGCTTCCGGAGCGCGAGCGCATGGTCCTGCTGCTGCGGTTCTTCGAGAACCTCACCCAGACCCAGATCGCCGAACGGGTC
GGCATCTCGCAGATGCACGTGTCCAGGCTGCTGGCCAAATCGCTAACGCGGCTCCGCGACCAGTTGCAGTAG

Upstream 100 bases:

>100_bases
CGCTGACCGATGAGGTGCGTACCTTCTCCAACGGTCATGCTCCCGGAGACGGTCAGGTATTCGGCATCTCGATGACGACG
AGACGAGCGAGTTCGCTGCG

Downstream 100 bases:

>100_bases
CGGGCGCGGTTCACCCTGCAGCGCGGTGAGCGCCGTGTCGACGCTTTCCGACCACGGCAGGGTGGCGTCCGGATCGCAGA
TCCGCAGCAGCCGGCTGACC

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTPSSAQGSSPRQNSEYSDVAPMFRELQGLTPSTPQFQRQRDRIVERCLPLADHIARRFDGRGEPRDDLVQVARVGLVNA
VIRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGSATAELSQRLGRAPTASELAEELGMDRDEVV
EGLVAGSSYNTLSIDSGGNSGNEDAPAIADTLGDVDLGLDQIENREALRPLLAALPERERMVLLLRFFENLTQTQIAERV
GISQMHVSRLLAKSLTRLRDQLQ

Sequences:

>Translated_263_residues
MTPSSAQGSSPRQNSEYSDVAPMFRELQGLTPSTPQFQRQRDRIVERCLPLADHIARRFDGRGEPRDDLVQVARVGLVNA
VIRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGSATAELSQRLGRAPTASELAEELGMDRDEVV
EGLVAGSSYNTLSIDSGGNSGNEDAPAIADTLGDVDLGLDQIENREALRPLLAALPERERMVLLLRFFENLTQTQIAERV
GISQMHVSRLLAKSLTRLRDQLQ
>Mature_262_residues
TPSSAQGSSPRQNSEYSDVAPMFRELQGLTPSTPQFQRQRDRIVERCLPLADHIARRFDGRGEPRDDLVQVARVGLVNAV
IRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGSATAELSQRLGRAPTASELAEELGMDRDEVVE
GLVAGSSYNTLSIDSGGNSGNEDAPAIADTLGDVDLGLDQIENREALRPLLAALPERERMVLLLRFFENLTQTQIAERVG
ISQMHVSRLLAKSLTRLRDQLQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=231, Percent_Identity=29.4372294372294, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=27.8761061946903, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789098, Length=236, Percent_Identity=28.8135593220339, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29214; Mature: 29083

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSSAQGSSPRQNSEYSDVAPMFRELQGLTPSTPQFQRQRDRIVERCLPLADHIARRFD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GRGEPRDDLVQVARVGLVNAVIRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRR
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
LKELHLRLGSATAELSQRLGRAPTASELAEELGMDRDEVVEGLVAGSSYNTLSIDSGGNS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCC
GNEDAPAIADTLGDVDLGLDQIENREALRPLLAALPERERMVLLLRFFENLTQTQIAERV
CCCCCHHHHHHHHHHHHCHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GISQMHVSRLLAKSLTRLRDQLQ
CHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPSSAQGSSPRQNSEYSDVAPMFRELQGLTPSTPQFQRQRDRIVERCLPLADHIARRFD
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GRGEPRDDLVQVARVGLVNAVIRFDVDAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRR
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
LKELHLRLGSATAELSQRLGRAPTASELAEELGMDRDEVVEGLVAGSSYNTLSIDSGGNS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCC
GNEDAPAIADTLGDVDLGLDQIENREALRPLLAALPERERMVLLLRFFENLTQTQIAERV
CCCCCHHHHHHHHHHHHCHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GISQMHVSRLLAKSLTRLRDQLQ
CHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]