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The map label for this gene is pdhC [H]

Identifier: 108798065

GI number: 108798065

Start: 1192306

End: 1193505

Strand: Direct

Name: pdhC [H]

Synonym: Mmcs_1093

Alternate gene names: 108798065

Gene position: 1192306-1193505 (Clockwise)

Preceding gene: 108798064

Following gene: 108798066

Centisome position: 20.9

GC content: 70.17

Gene sequence:

>1200_bases
ATGACCGAGTTCCGGATGCCCGCGCTCGGCTCGGACATGGACGAGGGGACCCTCGACCAATGGCTGGTCAAACCGGGCGA
CACCGTCACCAGGGGCCAGGTCGTGGCGGTGGTCGAGACCACCAAGGCCGCCGTCGAGGTCGAATGCTGGCAGGAGGGGA
CCGTCGACCGCCTCCTGGTGCCCGAAGGCCAGACCGTCCGGGTCGGAACGCCGCTGGCCACGCTGCTGGCTCCGGGCGAA
ACACCCGCACCGACTGCACCGGCGGTGCCTCGCACGATGCGGGAATCGCCGGTGGCCGTCGAGAGACCAGAAGGCGCAGG
CAGGCCTGCTCCGGCCGCGGGGCCAGCCATCGCGACCCGGCCGCATCGCCGGTGGGTCTCCCCGGCGGCCCGCCGCGTGG
CAGCGACGCTGGACATCGACGCCGATACCCTCACCGGCACCGGTCCGCAGGGCGCGGTCACCATTCGCGACGTGGAACAG
GCGGCAGCGTCGAGGAAGCAGCCGGCCGACGGGCGAACCGTACGGGATCGGTCCGTGGCGATGCGCGCGTCGATCGCCGC
GGCGATGAGCCGGTCGAAGCGCGAGATTCCGCACTACTACCTGGCCGACGAAGTCCTCATGGACCCGGCGCTGGCATGGC
TGGCTGAGCGCAACGCCGCGCGATCCATCACCGAACGGGTGTTGCCGGCGGTGCTGCAGATCAAGGCCGTCGCGGCGGCA
GCGGACCGCTTTCCCGAGTTCAACGGCTTCTGGCGCGACGACGCGTTCGTCGGTGCCGACGGCGTCCACGTCGGTGTCGC
CATCTCACTTCGCGGTGGGGGCCTGGTCGCACCCGCGATCCACGACGTCCCCGACAGGAGCCTCGACGACCTCATGGGGG
CCCTGACCGACCTGGTGGCGCGCGCCCGGGCCGGCTCGCTGCGCAGTTCGGAGATGTCTGATCCCTCCATCACGATCACC
AACCTGGGCGACCAGGGGGTGGACACGGTGTTCGGCGTCATCTATCCGCCACAGGTCGCCCTGGTGGGCTTCGGCAAGCC
GGTGCAACGGGTATGTGCCGTCGACGGTGGTATTCGTATCGCGACCGCGCTGACCGCCACTCTGGCAGCGGATCACCGGG
CCAGCGATGGACACCGCGGTGCGCTCTTCCTCGCCGCGATCAACGAGATCCTGCAGCAGCCGCAGAAGTTGGAGAAGTGA

Upstream 100 bases:

>100_bases
CAGAAGTCCCGGTGCCCTATGCCAAGCACCTCGAGCAGGCCGCCCTACCGCAGGCCGGTCAGATTGCGACCGCGGTCAGG
GACCTCTGCGGCGGCCCAGC

Downstream 100 bases:

>100_bases
CGCAGTGACGAAGAGCGAGCAGCAGATCCGCGATGACGTCGTGGCCGTGTTGACCAGGATCGCACCGGAGGTGGAGGCCG
ACCAGTTGGAGGAGAATGAG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTEFRMPALGSDMDEGTLDQWLVKPGDTVTRGQVVAVVETTKAAVEVECWQEGTVDRLLVPEGQTVRVGTPLATLLAPGE
TPAPTAPAVPRTMRESPVAVERPEGAGRPAPAAGPAIATRPHRRWVSPAARRVAATLDIDADTLTGTGPQGAVTIRDVEQ
AAASRKQPADGRTVRDRSVAMRASIAAAMSRSKREIPHYYLADEVLMDPALAWLAERNAARSITERVLPAVLQIKAVAAA
ADRFPEFNGFWRDDAFVGADGVHVGVAISLRGGGLVAPAIHDVPDRSLDDLMGALTDLVARARAGSLRSSEMSDPSITIT
NLGDQGVDTVFGVIYPPQVALVGFGKPVQRVCAVDGGIRIATALTATLAADHRASDGHRGALFLAAINEILQQPQKLEK

Sequences:

>Translated_399_residues
MTEFRMPALGSDMDEGTLDQWLVKPGDTVTRGQVVAVVETTKAAVEVECWQEGTVDRLLVPEGQTVRVGTPLATLLAPGE
TPAPTAPAVPRTMRESPVAVERPEGAGRPAPAAGPAIATRPHRRWVSPAARRVAATLDIDADTLTGTGPQGAVTIRDVEQ
AAASRKQPADGRTVRDRSVAMRASIAAAMSRSKREIPHYYLADEVLMDPALAWLAERNAARSITERVLPAVLQIKAVAAA
ADRFPEFNGFWRDDAFVGADGVHVGVAISLRGGGLVAPAIHDVPDRSLDDLMGALTDLVARARAGSLRSSEMSDPSITIT
NLGDQGVDTVFGVIYPPQVALVGFGKPVQRVCAVDGGIRIATALTATLAADHRASDGHRGALFLAAINEILQQPQKLEK
>Mature_398_residues
TEFRMPALGSDMDEGTLDQWLVKPGDTVTRGQVVAVVETTKAAVEVECWQEGTVDRLLVPEGQTVRVGTPLATLLAPGET
PAPTAPAVPRTMRESPVAVERPEGAGRPAPAAGPAIATRPHRRWVSPAARRVAATLDIDADTLTGTGPQGAVTIRDVEQA
AASRKQPADGRTVRDRSVAMRASIAAAMSRSKREIPHYYLADEVLMDPALAWLAERNAARSITERVLPAVLQIKAVAAAA
DRFPEFNGFWRDDAFVGADGVHVGVAISLRGGGLVAPAIHDVPDRSLDDLMGALTDLVARARAGSLRSSEMSDPSITITN
LGDQGVDTVFGVIYPPQVALVGFGKPVQRVCAVDGGIRIATALTATLAADHRASDGHRGALFLAAINEILQQPQKLEK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=426, Percent_Identity=28.8732394366197, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI203098753, Length=462, Percent_Identity=24.025974025974, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI203098816, Length=462, Percent_Identity=24.025974025974, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI19923748, Length=168, Percent_Identity=30.3571428571429, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1786946, Length=420, Percent_Identity=27.1428571428571, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=25.8215962441315, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=29.2343387470998, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=25.4117647058824, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25146366, Length=414, Percent_Identity=26.5700483091787, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17538894, Length=243, Percent_Identity=29.2181069958848, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=25.503355704698, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=25.8536585365854, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20129315, Length=221, Percent_Identity=31.6742081447964, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24582497, Length=221, Percent_Identity=31.6742081447964, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=23.963133640553, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24645909, Length=222, Percent_Identity=28.8288288288288, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 42145; Mature: 42014

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFRMPALGSDMDEGTLDQWLVKPGDTVTRGQVVAVVETTKAAVEVECWQEGTVDRLLV
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEC
PEGQTVRVGTPLATLLAPGETPAPTAPAVPRTMRESPVAVERPEGAGRPAPAAGPAIATR
CCCCEEEECCCHHHHCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
PHRRWVSPAARRVAATLDIDADTLTGTGPQGAVTIRDVEQAAASRKQPADGRTVRDRSVA
CCHHHCCHHHHHEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHH
MRASIAAAMSRSKREIPHYYLADEVLMDPALAWLAERNAARSITERVLPAVLQIKAVAAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRFPEFNGFWRDDAFVGADGVHVGVAISLRGGGLVAPAIHDVPDRSLDDLMGALTDLVA
HHCCCCCCCCCCCCCEECCCCEEEEEEEEECCCCEECCHHHCCCCCCHHHHHHHHHHHHH
RARAGSLRSSEMSDPSITITNLGDQGVDTVFGVIYPPQVALVGFGKPVQRVCAVDGGIRI
HHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHEECCCCEEEEECCHHHHHHHHCCCCEEE
ATALTATLAADHRASDGHRGALFLAAINEILQQPQKLEK
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEFRMPALGSDMDEGTLDQWLVKPGDTVTRGQVVAVVETTKAAVEVECWQEGTVDRLLV
CCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEC
PEGQTVRVGTPLATLLAPGETPAPTAPAVPRTMRESPVAVERPEGAGRPAPAAGPAIATR
CCCCEEEECCCHHHHCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
PHRRWVSPAARRVAATLDIDADTLTGTGPQGAVTIRDVEQAAASRKQPADGRTVRDRSVA
CCHHHCCHHHHHEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHH
MRASIAAAMSRSKREIPHYYLADEVLMDPALAWLAERNAARSITERVLPAVLQIKAVAAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRFPEFNGFWRDDAFVGADGVHVGVAISLRGGGLVAPAIHDVPDRSLDDLMGALTDLVA
HHCCCCCCCCCCCCCEECCCCEEEEEEEEECCCCEECCHHHCCCCCCHHHHHHHHHHHHH
RARAGSLRSSEMSDPSITITNLGDQGVDTVFGVIYPPQVALVGFGKPVQRVCAVDGGIRI
HHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHEECCCCEEEEECCHHHHHHHHCCCCEEE
ATALTATLAADHRASDGHRGALFLAAINEILQQPQKLEK
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA