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The map label for this gene is nfdA [H]

Identifier: 108797721

GI number: 108797721

Start: 787429

End: 789039

Strand: Direct

Name: nfdA [H]

Synonym: Mmcs_0742

Alternate gene names: 108797721

Gene position: 787429-789039 (Clockwise)

Preceding gene: 108797719

Following gene: 108797722

Centisome position: 13.8

GC content: 69.09

Gene sequence:

>1611_bases
ATGCCCGCCGCCGATCTCGTCCTGACCGGGACCGTGCTCACCGTCAACGACGCACAACCGACGGCCGAAGCGCTCGCGGT
GGCCGACGGGCGCATCGTCGCCGTGGGGGACCGCTCCGACGTCGAGACCTGGATCGGGCCCGGCACCGAGGTCCTCGACG
TGGGCGATGGCTGCGTGCTGCCCGGTCTTGTGGAGGCGCACGGGCATCCGCTGATGGAGGCGATCGCGCTGTCCGAGCGG
ATGGTCGACATCCGTCCCGTGACGATGAGGTCCGCCGACGAGGTCGTCGCCGCCGTGCGCAGCGAGGTGGCGCGGCGCGG
ACGACAGGGTGCCTACCTCAACGGGTGGGATCCGCTGCTGCAGGACGGGCTGCCGGACCCGACGTTGACGTGGCTCGACG
GCATCGCACCGGACAGCCCGCTGGTGATCCTGCACAACTCCGGACACAAGGCGTTCTTCAACAGCGTCGCGGCCAAACGG
GCGGGGCTCAACCGCGACGTCCCGGATCCGAAAGGCGCACGCTTCGGCCGCGACGCCGACGGCGAACTCGACGGCACCGC
CGAGGAGACCGCCGCGGTGTTCCCGCTGCTCGCCGGCGTCGTCTCACCGAGTGACTATCCGTCGATGCTGGTGGCCGAGT
GCGCGCGACTCAACCGCGCGGGGCTCACGACGTGTTCGGACATGGCGTTCGATCCGGTGTTCCGGCCCATGCTCGACGGT
CTGCGCGACGAGCTCACGATCCGGTTGCGCGCCTATGAGGTCTCCAACCCGCAGCTGACCAGCACCGCGCACCCGGGCGA
CGGCGACGACCTGGTGCGCCAGATCGGCATCAAGATCTGGGTTGACGGTTCGCCGTGGATCGGCAACGTCGACCTGACGT
TTCCCTATCTGGACACCGCGGCCACCCGGACCATCGGGGTGGTGCCGGGCTCGTGCGGTCATGCGAATTACACCCGCGAG
CAATTGCGCGAGATCGTCGGCGCCTACTTTCCGTTGGGCTGGCAGATGGCCTGTCACGTGCACGGCGACGCCGGGGTCGA
CACGATCCTCGATGTCTACGAGGAGGTGCTCGAGGCCCATCCCCGCCCCGATCATCGGCTGCGCCTCGAACACGTCGGCG
CCATCACCGACGAGCAGCTCGAGCGTGCGCACGCCCTCGGCGTCACCTGCAGCATCTTCGTCGACCAGATCCACTACTGG
GGGGACGTCATCGTCGACGGGCTGTTCGGCCCGGAACGCGGAAACCGTTGGATGCCTTGCGGATCGGCCGTCGCGACCGG
TATGCGGATCTCACTGCACAACGATCCGCCGGTCACCCCGGAGGAGCCGTTGCGCAACATCAGCGTCGCGGTGACGCGCA
CGGCGCCCAGCGGGCGGGTGCTCGCGCCCGAGGAGCGGCTGACCGTCGATCAGGCGATCCGCGCCCAGACCCTCGACGCC
GCGTGGCAGCTGTTCGCCGACGACGTGATCGGCTCGATCGAGGTGGGCAAGTACGGCGATCTGGTGGTGCTGTCGGCGGA
TCCGCGCAGCGTGCCGCCGGAGCGGATCGCCGAACTCGACGTGCTGGCCACCTATCTCTCGGGCCGCTGCGTTTACGCCA
AACAACTCTGA

Upstream 100 bases:

>100_bases
CGTCGAACGGCACCCCCGGAAAATCGACACGAAAAGAAATTTTTCTCGAAGTTTCTCGCGACTACCGAGAAACCGCAGGT
CATCGCATTAGCATTCGCCC

Downstream 100 bases:

>100_bases
CAACCACCGTTGTCATGGCAGGCTCGTAGGTATGCCCGGACTGCCTGACCCCCGCTTCCTCGACGACCGGCTCAACCACT
GGGCGCACACCACGCCCGAC

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MPAADLVLTGTVLTVNDAQPTAEALAVADGRIVAVGDRSDVETWIGPGTEVLDVGDGCVLPGLVEAHGHPLMEAIALSER
MVDIRPVTMRSADEVVAAVRSEVARRGRQGAYLNGWDPLLQDGLPDPTLTWLDGIAPDSPLVILHNSGHKAFFNSVAAKR
AGLNRDVPDPKGARFGRDADGELDGTAEETAAVFPLLAGVVSPSDYPSMLVAECARLNRAGLTTCSDMAFDPVFRPMLDG
LRDELTIRLRAYEVSNPQLTSTAHPGDGDDLVRQIGIKIWVDGSPWIGNVDLTFPYLDTAATRTIGVVPGSCGHANYTRE
QLREIVGAYFPLGWQMACHVHGDAGVDTILDVYEEVLEAHPRPDHRLRLEHVGAITDEQLERAHALGVTCSIFVDQIHYW
GDVIVDGLFGPERGNRWMPCGSAVATGMRISLHNDPPVTPEEPLRNISVAVTRTAPSGRVLAPEERLTVDQAIRAQTLDA
AWQLFADDVIGSIEVGKYGDLVVLSADPRSVPPERIAELDVLATYLSGRCVYAKQL

Sequences:

>Translated_536_residues
MPAADLVLTGTVLTVNDAQPTAEALAVADGRIVAVGDRSDVETWIGPGTEVLDVGDGCVLPGLVEAHGHPLMEAIALSER
MVDIRPVTMRSADEVVAAVRSEVARRGRQGAYLNGWDPLLQDGLPDPTLTWLDGIAPDSPLVILHNSGHKAFFNSVAAKR
AGLNRDVPDPKGARFGRDADGELDGTAEETAAVFPLLAGVVSPSDYPSMLVAECARLNRAGLTTCSDMAFDPVFRPMLDG
LRDELTIRLRAYEVSNPQLTSTAHPGDGDDLVRQIGIKIWVDGSPWIGNVDLTFPYLDTAATRTIGVVPGSCGHANYTRE
QLREIVGAYFPLGWQMACHVHGDAGVDTILDVYEEVLEAHPRPDHRLRLEHVGAITDEQLERAHALGVTCSIFVDQIHYW
GDVIVDGLFGPERGNRWMPCGSAVATGMRISLHNDPPVTPEEPLRNISVAVTRTAPSGRVLAPEERLTVDQAIRAQTLDA
AWQLFADDVIGSIEVGKYGDLVVLSADPRSVPPERIAELDVLATYLSGRCVYAKQL
>Mature_535_residues
PAADLVLTGTVLTVNDAQPTAEALAVADGRIVAVGDRSDVETWIGPGTEVLDVGDGCVLPGLVEAHGHPLMEAIALSERM
VDIRPVTMRSADEVVAAVRSEVARRGRQGAYLNGWDPLLQDGLPDPTLTWLDGIAPDSPLVILHNSGHKAFFNSVAAKRA
GLNRDVPDPKGARFGRDADGELDGTAEETAAVFPLLAGVVSPSDYPSMLVAECARLNRAGLTTCSDMAFDPVFRPMLDGL
RDELTIRLRAYEVSNPQLTSTAHPGDGDDLVRQIGIKIWVDGSPWIGNVDLTFPYLDTAATRTIGVVPGSCGHANYTREQ
LREIVGAYFPLGWQMACHVHGDAGVDTILDVYEEVLEAHPRPDHRLRLEHVGAITDEQLERAHALGVTCSIFVDQIHYWG
DVIVDGLFGPERGNRWMPCGSAVATGMRISLHNDPPVTPEEPLRNISVAVTRTAPSGRVLAPEERLTVDQAIRAQTLDAA
WQLFADDVIGSIEVGKYGDLVVLSADPRSVPPERIAELDVLATYLSGRCVYAKQL

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 57746; Mature: 57615

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAADLVLTGTVLTVNDAQPTAEALAVADGRIVAVGDRSDVETWIGPGTEVLDVGDGCVL
CCCCCEEEEEEEEEECCCCCHHHHHEECCCEEEEECCCCCCHHHCCCCCEEEECCCCCCC
PGLVEAHGHPLMEAIALSERMVDIRPVTMRSADEVVAAVRSEVARRGRQGAYLNGWDPLL
CCHHHHCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
QDGLPDPTLTWLDGIAPDSPLVILHNSGHKAFFNSVAAKRAGLNRDVPDPKGARFGRDAD
HCCCCCCHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GELDGTAEETAAVFPLLAGVVSPSDYPSMLVAECARLNRAGLTTCSDMAFDPVFRPMLDG
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
LRDELTIRLRAYEVSNPQLTSTAHPGDGDDLVRQIGIKIWVDGSPWIGNVDLTFPYLDTA
CCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCC
ATRTIGVVPGSCGHANYTREQLREIVGAYFPLGWQMACHVHGDAGVDTILDVYEEVLEAH
CCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCC
PRPDHRLRLEHVGAITDEQLERAHALGVTCSIFVDQIHYWGDVIVDGLFGPERGNRWMPC
CCCCCEEEHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
GSAVATGMRISLHNDPPVTPEEPLRNISVAVTRTAPSGRVLAPEERLTVDQAIRAQTLDA
CHHHHCCEEEEECCCCCCCHHHHHCCEEEEEEEECCCCCEECCHHHHHHHHHHHHHHHHH
AWQLFADDVIGSIEVGKYGDLVVLSADPRSVPPERIAELDVLATYLSGRCVYAKQL
HHHHHHHHHHCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
PAADLVLTGTVLTVNDAQPTAEALAVADGRIVAVGDRSDVETWIGPGTEVLDVGDGCVL
CCCCEEEEEEEEEECCCCCHHHHHEECCCEEEEECCCCCCHHHCCCCCEEEECCCCCCC
PGLVEAHGHPLMEAIALSERMVDIRPVTMRSADEVVAAVRSEVARRGRQGAYLNGWDPLL
CCHHHHCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
QDGLPDPTLTWLDGIAPDSPLVILHNSGHKAFFNSVAAKRAGLNRDVPDPKGARFGRDAD
HCCCCCCHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GELDGTAEETAAVFPLLAGVVSPSDYPSMLVAECARLNRAGLTTCSDMAFDPVFRPMLDG
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
LRDELTIRLRAYEVSNPQLTSTAHPGDGDDLVRQIGIKIWVDGSPWIGNVDLTFPYLDTA
CCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCC
ATRTIGVVPGSCGHANYTREQLREIVGAYFPLGWQMACHVHGDAGVDTILDVYEEVLEAH
CCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCC
PRPDHRLRLEHVGAITDEQLERAHALGVTCSIFVDQIHYWGDVIVDGLFGPERGNRWMPC
CCCCCEEEHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
GSAVATGMRISLHNDPPVTPEEPLRNISVAVTRTAPSGRVLAPEERLTVDQAIRAQTLDA
CHHHHCCEEEEECCCCCCCHHHHHCCEEEEEEEECCCCCEECCHHHHHHHHHHHHHHHHH
AWQLFADDVIGSIEVGKYGDLVVLSADPRSVPPERIAELDVLATYLSGRCVYAKQL
HHHHHHHHHHCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA