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The map label for this gene is pit [H]

Identifier: 108797707

GI number: 108797707

Start: 776528

End: 777778

Strand: Direct

Name: pit [H]

Synonym: Mmcs_0728

Alternate gene names: 108797707

Gene position: 776528-777778 (Clockwise)

Preceding gene: 108797704

Following gene: 108797708

Centisome position: 13.61

GC content: 68.75

Gene sequence:

>1251_bases
GTGAGTTCCGAGTGGATCATCTTGGTGCTGCTCATCGCGACAGCACTGGCTTTCGACTTCACCAACGGTTTCCACGACAC
CGGAAACGCGATGGCCACGTCGATCGCCACCGGCGCGTTGAAGCCCAAGACTGCCGTGACGCTGGCCGGCGTGCTCAACC
TGGTCGGCGCGTTCCTCTCGGTGGAAGTGGCCGTCACCGTCACCACCTCGGTGCTCAAGATCCAGGACAGCAAGACCGGA
TCGCTGCTGCCGAACATCGACGCCTCCACCGGCCTGACCATCATCTTCGCCGGGCTGGTCGGCGGCATCCTGTGGAACCT
GCTGACGTGGTTGTTCGGCATCCCGTCGAGTTCGTCGCACGCGCTGTTCGGCGGGCTGATGGGCGCGGGCCTTGCCGCGA
TCGGGCTGGCGGGCGTCAACTGGTCGGGCGTCACCCAGAAGGTGCTCATCCCCGCGATCGCCGCACCCGTCATCGCCGGT
CTGGTCGCCGGATGCGGCACCTGGCTGATCTACCGCATCACCCGAAGGGTGGGACGCAAGCAGCGCGAATCGGGCTTCCG
GTGGGGCCAGATCGCCACCGCGTCGCTGGTCGCGCTGTCCCACGGCACCAACGACGCGCAGAAGACCATGGGCGTCATCG
CGCTGGCCCTGATCACCACCGGCCACCTGACCGGCGACGTCGCCGAGGACGGTCTGCCGTTCTGGATCATCGCCAGCTGC
GCACTGGCCATCGGCCTCGGCACCTACCTCGGAGGTTGGCGGGTCATCCGCACACTCGGCAAGGGTCTGGTCGAGATCGA
GTCGCCGCAGGGCCTGGCCGCCGAGGCGTCGTCCGCCGCGATCATCCTCAGCTCCAGCGCCGCGGGGATGGCGCTGTCGA
CCACGCACGTCGCCACGGGTTCCATCCTCGGCAGCGGTGTCGGCAAGCCGGGTGCGCACGTCCGCTGGGCCGTCGCCGGC
CGGATGGCGGTGGCCTGGCTGATCACCCTGCCCGCCGCGGGTCTCGTCGGGGCGCTGGCCTTCTGGGTCTCCCACTCGGT
GGCCGGGATGACGACCCAGCTCGTGGGCGACGGCCTGATCTTCCTGATCCTGGTCGGCACGTCGTTCGGTCTGTGGTGGC
GCGCCCAGCAGCAGAAGGTCGACCACCGCAACGTCAACGCCGACTGGGACGCCAGCACCAATTCCGTGGTGCCCGTCGAG
GTGCGGGAGCCCGTGCCCGTCGCAAAGGCCAAAGACGTCGCGGCCGTCTGA

Upstream 100 bases:

>100_bases
GTGAACAACCCACCGAAGCCGCCGAAAGCCCAGGACACACCGGAAATTAGGCGCGCTAGTTTTTGGTTTTGGAAGCCATG
GCCCAGCAGAATGTGGACTC

Downstream 100 bases:

>100_bases
GACCCGAGAACGACTGAAGGACATACCGAATGGTTAACCTCGAGAGCATCCTGCGGGTGCTGGTCGTCGGCCTGATCCTC
GGTGCGGGGCTGCCCGCGCT

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSSEWIILVLLIATALAFDFTNGFHDTGNAMATSIATGALKPKTAVTLAGVLNLVGAFLSVEVAVTVTTSVLKIQDSKTG
SLLPNIDASTGLTIIFAGLVGGILWNLLTWLFGIPSSSSHALFGGLMGAGLAAIGLAGVNWSGVTQKVLIPAIAAPVIAG
LVAGCGTWLIYRITRRVGRKQRESGFRWGQIATASLVALSHGTNDAQKTMGVIALALITTGHLTGDVAEDGLPFWIIASC
ALAIGLGTYLGGWRVIRTLGKGLVEIESPQGLAAEASSAAIILSSSAAGMALSTTHVATGSILGSGVGKPGAHVRWAVAG
RMAVAWLITLPAAGLVGALAFWVSHSVAGMTTQLVGDGLIFLILVGTSFGLWWRAQQQKVDHRNVNADWDASTNSVVPVE
VREPVPVAKAKDVAAV

Sequences:

>Translated_416_residues
MSSEWIILVLLIATALAFDFTNGFHDTGNAMATSIATGALKPKTAVTLAGVLNLVGAFLSVEVAVTVTTSVLKIQDSKTG
SLLPNIDASTGLTIIFAGLVGGILWNLLTWLFGIPSSSSHALFGGLMGAGLAAIGLAGVNWSGVTQKVLIPAIAAPVIAG
LVAGCGTWLIYRITRRVGRKQRESGFRWGQIATASLVALSHGTNDAQKTMGVIALALITTGHLTGDVAEDGLPFWIIASC
ALAIGLGTYLGGWRVIRTLGKGLVEIESPQGLAAEASSAAIILSSSAAGMALSTTHVATGSILGSGVGKPGAHVRWAVAG
RMAVAWLITLPAAGLVGALAFWVSHSVAGMTTQLVGDGLIFLILVGTSFGLWWRAQQQKVDHRNVNADWDASTNSVVPVE
VREPVPVAKAKDVAAV
>Mature_415_residues
SSEWIILVLLIATALAFDFTNGFHDTGNAMATSIATGALKPKTAVTLAGVLNLVGAFLSVEVAVTVTTSVLKIQDSKTGS
LLPNIDASTGLTIIFAGLVGGILWNLLTWLFGIPSSSSHALFGGLMGAGLAAIGLAGVNWSGVTQKVLIPAIAAPVIAGL
VAGCGTWLIYRITRRVGRKQRESGFRWGQIATASLVALSHGTNDAQKTMGVIALALITTGHLTGDVAEDGLPFWIIASCA
LAIGLGTYLGGWRVIRTLGKGLVEIESPQGLAAEASSAAIILSSSAAGMALSTTHVATGSILGSGVGKPGAHVRWAVAGR
MAVAWLITLPAAGLVGALAFWVSHSVAGMTTQLVGDGLIFLILVGTSFGLWWRAQQQKVDHRNVNADWDASTNSVVPVEV
REPVPVAKAKDVAAV

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=155, Percent_Identity=32.9032258064516, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI5803173, Length=154, Percent_Identity=33.7662337662338, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789360, Length=226, Percent_Identity=34.070796460177, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789907, Length=226, Percent_Identity=34.070796460177, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71981576, Length=181, Percent_Identity=30.939226519337, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25146401, Length=173, Percent_Identity=31.7919075144509, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=206, Percent_Identity=28.1553398058252, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17539280, Length=152, Percent_Identity=29.6052631578947, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536725, Length=165, Percent_Identity=28.4848484848485, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=153, Percent_Identity=28.7581699346405, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21356511, Length=214, Percent_Identity=28.9719626168224, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 42725; Mature: 42594

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEWIILVLLIATALAFDFTNGFHDTGNAMATSIATGALKPKTAVTLAGVLNLVGAFLS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VEVAVTVTTSVLKIQDSKTGSLLPNIDASTGLTIIFAGLVGGILWNLLTWLFGIPSSSSH
EEEEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ALFGGLMGAGLAAIGLAGVNWSGVTQKVLIPAIAAPVIAGLVAGCGTWLIYRITRRVGRK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRESGFRWGQIATASLVALSHGTNDAQKTMGVIALALITTGHLTGDVAEDGLPFWIIASC
HHHCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHH
ALAIGLGTYLGGWRVIRTLGKGLVEIESPQGLAAEASSAAIILSSSAAGMALSTTHVATG
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEEHHHHHC
SILGSGVGKPGAHVRWAVAGRMAVAWLITLPAAGLVGALAFWVSHSVAGMTTQLVGDGLI
HHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FLILVGTSFGLWWRAQQQKVDHRNVNADWDASTNSVVPVEVREPVPVAKAKDVAAV
EEEEECCCCCCEEEHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSEWIILVLLIATALAFDFTNGFHDTGNAMATSIATGALKPKTAVTLAGVLNLVGAFLS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VEVAVTVTTSVLKIQDSKTGSLLPNIDASTGLTIIFAGLVGGILWNLLTWLFGIPSSSSH
EEEEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ALFGGLMGAGLAAIGLAGVNWSGVTQKVLIPAIAAPVIAGLVAGCGTWLIYRITRRVGRK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRESGFRWGQIATASLVALSHGTNDAQKTMGVIALALITTGHLTGDVAEDGLPFWIIASC
HHHCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHH
ALAIGLGTYLGGWRVIRTLGKGLVEIESPQGLAAEASSAAIILSSSAAGMALSTTHVATG
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEEHHHHHC
SILGSGVGKPGAHVRWAVAGRMAVAWLITLPAAGLVGALAFWVSHSVAGMTTQLVGDGLI
HHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FLILVGTSFGLWWRAQQQKVDHRNVNADWDASTNSVVPVEVREPVPVAKAKDVAAV
EEEEECCCCCCEEEHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]