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The map label for this gene is galE [C]

Identifier: 108797651

GI number: 108797651

Start: 729649

End: 730722

Strand: Direct

Name: galE [C]

Synonym: Mmcs_0671

Alternate gene names: 108797651

Gene position: 729649-730722 (Clockwise)

Preceding gene: 108797650

Following gene: 108797652

Centisome position: 12.79

GC content: 67.78

Gene sequence:

>1074_bases
GTGGTGGATTCGAGCACCCCGGGCGACGTCGGTTCGTACCCGAAGGTGGTCCTGGTCACCGGGGCGTGCCGGTTCCTCGG
CGGCTATCTGATCGCGCGGTTGGCGCAGAACCCCTTGATCAACCATGTGATCGCGGTCGACGCGATCGCGCCGAGCAAGG
ATCTGCTGCGGCGAATGGGCCGCGCCGAGTTCGTCCGCGCGGACATTCGTAATCCGTTCATCGCCAAGGTGATTCGCAAC
GGCGATGTGGACACGGTGGTACACGCGGCCGCCGCGTCGTACGCCCCGCGCGCAGGTGGCCGGGCCGCGTTGAAAGAACT
CAACGTGATGGGCGCGATCCAGCTCTTCGCCGCCTGCCAGAAGGCGCCTGCGGTACGGCGGGTGATCCTGAAGTCCACCT
CGGAGGTGTACGGCTCGAGTCCGCGCGACCCGGTGATGTTCACCGAGGACAGCGACGCGCGCCGGCCACCCGGCGAGGGC
TTCGCCCGCGACAGCATCGACATCGAGGGCTACGCCCGCGGCCTCGGGAGGCGCCGCCCCGACATCGGGGTCACCATCCT
GCGGCTGGCCAACATGATCGGGCCGGCGATGGACACCGCCCTGTCGCGGTATCTGGCCGGACCCGTCGTCCCGACGGTGC
TCGGACAGGACCCGCGGCTGCAGCTGCTGCACGAACAGGACGCACTGGGTGCGCTGGAACGCGCGACGACGGCGGGTAAG
TCCGGCACGTTCAACATCGGCGCCTCCGGAATCATCATGATGAGCCAGGCGATCCGGCGTTCGGGCCGAGTCGCGCTGCC
GGTCCCGCGTTCGGCTCTTTCGCTCGTGGACTCCTTGCGACGGGCAACCCGATACACTGAGCTCGATCGGGAGCAGCTGA
ACTATCTGAGCTACGGCCGGGTCATGGACACCACTCGCATGCGCGAAGTCCTCGAATACCACCCGAAGTGGACCACGGCG
GAGGCTTTCGACGACTACGTGCGTGGCCGTGGATTGACGCCCATCGTGGATCCACGGTGGGTACGGTCGGTGGAAGACCG
CGCCGTGGGGGTGGCGCAACGGTGGGGACGTTAG

Upstream 100 bases:

>100_bases
CGCAAGCTGCTTCGTCGGACACGGGTGCAGCGCAGAAAACTCGGTAAGTAGTCTGCCGGCCGTCGCCCAGGTGGCTCTCA
GCCGCAGCCGTTAGGCTGGC

Downstream 100 bases:

>100_bases
AGACAACTGACGAATCGGTGGGGTGAAGAGTACGTGTCTGGCGAGTCGAAGGCCAAAGTCATCCCGCTGCATGGGACGTC
GGGGCGGAACGCGGCGAGGC

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MVDSSTPGDVGSYPKVVLVTGACRFLGGYLIARLAQNPLINHVIAVDAIAPSKDLLRRMGRAEFVRADIRNPFIAKVIRN
GDVDTVVHAAAASYAPRAGGRAALKELNVMGAIQLFAACQKAPAVRRVILKSTSEVYGSSPRDPVMFTEDSDARRPPGEG
FARDSIDIEGYARGLGRRRPDIGVTILRLANMIGPAMDTALSRYLAGPVVPTVLGQDPRLQLLHEQDALGALERATTAGK
SGTFNIGASGIIMMSQAIRRSGRVALPVPRSALSLVDSLRRATRYTELDREQLNYLSYGRVMDTTRMREVLEYHPKWTTA
EAFDDYVRGRGLTPIVDPRWVRSVEDRAVGVAQRWGR

Sequences:

>Translated_357_residues
MVDSSTPGDVGSYPKVVLVTGACRFLGGYLIARLAQNPLINHVIAVDAIAPSKDLLRRMGRAEFVRADIRNPFIAKVIRN
GDVDTVVHAAAASYAPRAGGRAALKELNVMGAIQLFAACQKAPAVRRVILKSTSEVYGSSPRDPVMFTEDSDARRPPGEG
FARDSIDIEGYARGLGRRRPDIGVTILRLANMIGPAMDTALSRYLAGPVVPTVLGQDPRLQLLHEQDALGALERATTAGK
SGTFNIGASGIIMMSQAIRRSGRVALPVPRSALSLVDSLRRATRYTELDREQLNYLSYGRVMDTTRMREVLEYHPKWTTA
EAFDDYVRGRGLTPIVDPRWVRSVEDRAVGVAQRWGR
>Mature_357_residues
MVDSSTPGDVGSYPKVVLVTGACRFLGGYLIARLAQNPLINHVIAVDAIAPSKDLLRRMGRAEFVRADIRNPFIAKVIRN
GDVDTVVHAAAASYAPRAGGRAALKELNVMGAIQLFAACQKAPAVRRVILKSTSEVYGSSPRDPVMFTEDSDARRPPGEG
FARDSIDIEGYARGLGRRRPDIGVTILRLANMIGPAMDTALSRYLAGPVVPTVLGQDPRLQLLHEQDALGALERATTAGK
SGTFNIGASGIIMMSQAIRRSGRVALPVPRSALSLVDSLRRATRYTELDREQLNYLSYGRVMDTTRMREVLEYHPKWTTA
EAFDDYVRGRGLTPIVDPRWVRSVEDRAVGVAQRWGR

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 38994; Mature: 38994

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDSSTPGDVGSYPKVVLVTGACRFLGGYLIARLAQNPLINHVIAVDAIAPSKDLLRRMG
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHC
RAEFVRADIRNPFIAKVIRNGDVDTVVHAAAASYAPRAGGRAALKELNVMGAIQLFAACQ
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
KAPAVRRVILKSTSEVYGSSPRDPVMFTEDSDARRPPGEGFARDSIDIEGYARGLGRRRP
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
DIGVTILRLANMIGPAMDTALSRYLAGPVVPTVLGQDPRLQLLHEQDALGALERATTAGK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
SGTFNIGASGIIMMSQAIRRSGRVALPVPRSALSLVDSLRRATRYTELDREQLNYLSYGR
CCCEECCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMDTTRMREVLEYHPKWTTAEAFDDYVRGRGLTPIVDPRWVRSVEDRAVGVAQRWGR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVDSSTPGDVGSYPKVVLVTGACRFLGGYLIARLAQNPLINHVIAVDAIAPSKDLLRRMG
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHC
RAEFVRADIRNPFIAKVIRNGDVDTVVHAAAASYAPRAGGRAALKELNVMGAIQLFAACQ
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
KAPAVRRVILKSTSEVYGSSPRDPVMFTEDSDARRPPGEGFARDSIDIEGYARGLGRRRP
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
DIGVTILRLANMIGPAMDTALSRYLAGPVVPTVLGQDPRLQLLHEQDALGALERATTAGK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
SGTFNIGASGIIMMSQAIRRSGRVALPVPRSALSLVDSLRRATRYTELDREQLNYLSYGR
CCCEECCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMDTTRMREVLEYHPKWTTAEAFDDYVRGRGLTPIVDPRWVRSVEDRAVGVAQRWGR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]