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The map label for this gene is fadB2 [H]

Identifier: 108797617

GI number: 108797617

Start: 701114

End: 701974

Strand: Direct

Name: fadB2 [H]

Synonym: Mmcs_0637

Alternate gene names: 108797617

Gene position: 701114-701974 (Clockwise)

Preceding gene: 108797616

Following gene: 108797621

Centisome position: 12.29

GC content: 67.02

Gene sequence:

>861_bases
GTGAGCAATCCCATCGAACGAGTAGGCGTGATCGGCGCGGGGCAGATGGGCGCCGGCATCGCCGAGGTGTCCGCCCGCGC
GGGCGTCGAGGTACTGGTCTTCGAGACCACCGACGCGCTGGTCACCGCGGGTCGCAACCGCATCACCAAATCGTTGGAGC
GTGGGGTGAGCGCGGGCAAGGTCACCGATCGGGAGAAGTCGGCGGCGCTCGGGAAGCTGAGCTTCACCACCGACCTCGCC
GACATGGCGGACCGCCAGCTCGTCATCGAGGCGATCATCGAGGATGTGGCGATCAAGGCCAAGGTGTTCGCCGAACTCGA
CAAGGTCATCACCGACCCCGACGCGGTGCTGGCGTCGAACACCTCGAGCATCCCCATCATGAAACTCGCTGCGGCGACGC
AGAATTCGGGCCGAGTGCTGGGCCTGCACTTCTTCAACCCGGTGCCGGTGCTGCCGCTGGTCGAGCTGGTCAGCACGCTG
ACGACCACCGATGCGGCGATCGCGCGGACCGAGGCGTTCGCCAGCGACGTGCTCGGTAAGCAGGTGGTGCGCTGCAGCGA
CCGGTCGGGCTTCGTGGTCAACGCGCTGCTGGTGCCGTATCTGCTGTCGGCGATCCGGATGGTCGAGGCGGGCTTCGCCA
CCGTCGAAGATGTCGACAAGGCGGTCGTGGCCGGGCTGTCGCATCCGATGGGCCCGCTGCGGCTGAGCGACCTGATCGGC
CTGGACACCATGAAGCTGATCGCCGACGCGATGTTCGACGAGCTCAAGGACGCGCACTACGCCCCGCCGCCGCTGCTGCT
GCGCATGGTCGAGGCGGGTCAGCTGGGCAAGAAGTCGGGCCAAGGCTTCTACTCCTACTAG

Upstream 100 bases:

>100_bases
GTTCCACTAAGTCGTTGACCGCCGAGCGCACGGTTGTTGTACGCAAACGTCGAATAGACGTGACAACAACCGTGCGCTCG
TCGTCTGTTAGGAAGTAGCA

Downstream 100 bases:

>100_bases
CGCCGAGTGCACGCTTGTTGTACGCATCGGCCGAGTAGGGCCGTCAACAACCGTGCGCTCGACGAGCGGCCGTCACCCGG
TCGATGATCTCGGCCCGGCG

Product: 3-hydroxybutyryl-CoA dehydrogenase

Products: NA

Alternate protein names: Beta-hydroxybutyryl-CoA dehydrogenase; BHBD [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSNPIERVGVIGAGQMGAGIAEVSARAGVEVLVFETTDALVTAGRNRITKSLERGVSAGKVTDREKSAALGKLSFTTDLA
DMADRQLVIEAIIEDVAIKAKVFAELDKVITDPDAVLASNTSSIPIMKLAAATQNSGRVLGLHFFNPVPVLPLVELVSTL
TTTDAAIARTEAFASDVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLIG
LDTMKLIADAMFDELKDAHYAPPPLLLRMVEAGQLGKKSGQGFYSY

Sequences:

>Translated_286_residues
MSNPIERVGVIGAGQMGAGIAEVSARAGVEVLVFETTDALVTAGRNRITKSLERGVSAGKVTDREKSAALGKLSFTTDLA
DMADRQLVIEAIIEDVAIKAKVFAELDKVITDPDAVLASNTSSIPIMKLAAATQNSGRVLGLHFFNPVPVLPLVELVSTL
TTTDAAIARTEAFASDVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLIG
LDTMKLIADAMFDELKDAHYAPPPLLLRMVEAGQLGKKSGQGFYSY
>Mature_285_residues
SNPIERVGVIGAGQMGAGIAEVSARAGVEVLVFETTDALVTAGRNRITKSLERGVSAGKVTDREKSAALGKLSFTTDLAD
MADRQLVIEAIIEDVAIKAKVFAELDKVITDPDAVLASNTSSIPIMKLAAATQNSGRVLGLHFFNPVPVLPLVELVSTLT
TTDAAIARTEAFASDVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLIGL
DTMKLIADAMFDELKDAHYAPPPLLLRMVEAGQLGKKSGQGFYSY

Specific function: Catalyzes the NAD-dependent dehydration of beta- hydroxybutyryl-CoA to acetoacetyl-CoA in vitro at pH 10. Also catalyzes the reverse reaction albeit in a lower pH range of 5.5- 6.5. The reverse reaction is able to use NADPH as well as NADH [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI296179429, Length=290, Percent_Identity=40.6896551724138, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI296179427, Length=307, Percent_Identity=38.4364820846906, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI20127408, Length=285, Percent_Identity=31.5789473684211, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI261878539, Length=299, Percent_Identity=31.438127090301, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI68989263, Length=299, Percent_Identity=31.438127090301, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI115430219, Length=255, Percent_Identity=28.2352941176471, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787661, Length=283, Percent_Identity=39.9293286219081, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788682, Length=227, Percent_Identity=37.0044052863436, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1790281, Length=266, Percent_Identity=32.7067669172932, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17549919, Length=290, Percent_Identity=40.3448275862069, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17553560, Length=292, Percent_Identity=38.013698630137, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17563036, Length=295, Percent_Identity=37.9661016949153, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17558304, Length=284, Percent_Identity=32.7464788732394, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25144276, Length=284, Percent_Identity=33.0985915492958, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17508951, Length=284, Percent_Identity=33.0985915492958, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17508953, Length=284, Percent_Identity=33.0985915492958, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71985923, Length=285, Percent_Identity=29.4736842105263, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71985930, Length=287, Percent_Identity=28.2229965156794, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI115533614, Length=175, Percent_Identity=33.7142857142857, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193203623, Length=175, Percent_Identity=33.1428571428571, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583077, Length=287, Percent_Identity=33.1010452961672, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24583079, Length=287, Percent_Identity=33.1010452961672, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19921000, Length=287, Percent_Identity=33.1010452961672, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45549573, Length=269, Percent_Identity=28.996282527881, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20129993, Length=248, Percent_Identity=25, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022694
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N [H]

EC number: =1.1.1.157 [H]

Molecular weight: Translated: 30211; Mature: 30080

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPIERVGVIGAGQMGAGIAEVSARAGVEVLVFETTDALVTAGRNRITKSLERGVSAGK
CCCCHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEECCHHHHHCCHHHHHHHHHHCCCCCC
VTDREKSAALGKLSFTTDLADMADRQLVIEAIIEDVAIKAKVFAELDKVITDPDAVLASN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
TSSIPIMKLAAATQNSGRVLGLHFFNPVPVLPLVELVSTLTTTDAAIARTEAFASDVLGK
CCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLIG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHC
LDTMKLIADAMFDELKDAHYAPPPLLLRMVEAGQLGKKSGQGFYSY
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNPIERVGVIGAGQMGAGIAEVSARAGVEVLVFETTDALVTAGRNRITKSLERGVSAGK
CCCHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEECCHHHHHCCHHHHHHHHHHCCCCCC
VTDREKSAALGKLSFTTDLADMADRQLVIEAIIEDVAIKAKVFAELDKVITDPDAVLASN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
TSSIPIMKLAAATQNSGRVLGLHFFNPVPVLPLVELVSTLTTTDAAIARTEAFASDVLGK
CCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLIG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHC
LDTMKLIADAMFDELKDAHYAPPPLLLRMVEAGQLGKKSGQGFYSY
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]