Click here to switch to the map view.

The map label for this gene is fadE25 [H]

Identifier: 108797021

GI number: 108797021

Start: 50958

End: 53174

Strand: Direct

Name: fadE25 [H]

Synonym: Mmcs_0040

Alternate gene names: 108797021

Gene position: 50958-53174 (Clockwise)

Preceding gene: 108797020

Following gene: 108797022

Centisome position: 0.89

GC content: 71.76

Gene sequence:

>2217_bases
ATGCCGATCGCCCTGACCCCGGAACAGCGGGCGCTCTCCGACGCGGTGCGCGAGTTCGCCGCCCGCCACGCCCCGGTCGA
GAAGACCCGCCAGGCCTTCGACGACCTGGCCGCCGGCAACGTCCCGACCTGGTGGGATGACCTGGTGTCCGCCGGATTCC
ACGCCGTCCACCTGCCCGAGCGCGTGGGCGGGCAGGGCGGGGACCTGGTCGACACCGCCTGTGTGGTCGAGGCGGCCGCG
CAGGCGATGCTGCCCGGCCCGCTGCTGTCCACGGTGGCCACGGGCTCCGTCGCGCTGCTCGCCGACACGGTCACGTCCGA
ATCGCTGCTACGCGACCTCGCGGGCGGCACGACCGCGGCCGTCATCCTCCCCAATGACGCTGCGCTGCAGGCGGTATCGA
CACCCGACGGGTGGGAGGTCTCGGGTGTCTCCCCGGTGGTGCTCGGCGTGGGCGCCGCACGCCTGCTGCTGGCCGCGGCA
CACACCGAGGACGGCGGACTGATCTGGTTCGTCGCCGACGCCGACGCCGCGGTGACCGAATGCGAGCGGGGGACCGACCT
CACCACCGACGTGGCCCGCGTCCGGTTCGACGGGCACCAGGTGGCGTCCGGGGCCAGACTGACCGGGATCGACACCGAGC
GCGCCCGGTGCGCCGTCGCGGCGCTGACGGCGTCCGCGTCGGCGGGCACCATCCGGTGGTGCGTCGACGCCGTCACCGAA
CATCTGCGCACCCGTGAACAATTCGGCAAACCGATCGGCACGTTCCAGGCGTTGCAGCACCGCGCCGCGGACCTGCTGAT
CAGCAGCGAACTGGCCACCGCCGCCGCATGGGATGCGGTGCGCTCCGCCGACGAACCCGCCGAGCAGCACGTGATCGCCG
CGGCGACCGCCGTCCTGATGGCGGCCGCGCCCGCGCCCGACCTCGTCCTGGACACCCTGACGATGTTCGGCGCCATCGGT
TTCACCTGGGAACACGACCTGCACCTCTACTGGCGGCGCGCCACGGCGCTGGCGGCGTCGCTCGGTCCGGCGACGCGGTG
GAGCAGGCGACTCGGTGAGCTCACCCGGACCACCGCCAGGGACGTCACCGTGCGACTCGGTGACGCCGACCCCGAGTTCC
GGGCCCGCGTCAGCGACGTCCTGGACCGCGCGTCGACACTGCCCAACGACGGACCCCGCCCGCAGGGCGACTACGACCAG
TTCGCGACCGGCCCCCAGCGCACCGCCATCGCCGAAGCAGGTCTGATCGCCCCGCACTACCCGGCGCCCTGGGGACTCGA
CGCCACACCGCTGCAGCAGTTGATCATCGACGAGGAGTTCGCGGCCCGGCCGGCCCTGACCCGCCCGTCACTCGGCATCG
CCGAATGGATCGTCCCCTCGCTGATCAGCGCGGCCGGGCAGGAGGTGCGCGACCGCCTCATCCCCCCGACCCTGCGCGGT
GAGACGGCCTGGTGCCAGTTGTTCAGCGAACCGTCGGCCGGATCCGATCTGGCGTCGCTGACCACCAGGGCCACCAGGGT
GGACGGCGGCTGGAAGATCAACGGCCACAAGATCTGGACCTCGTCGGCGCAGCACGCGCACTTCGGCGCGCTGCTCGCCC
GCACCGATCCCGACGCCCGCAAGCATCGCGGCATCGGCTACTTCGTCGTCGACATGAGCTCCGAAGGTGTTGAGGTGCAA
CCTATCCGGCAGGTCACCGGAGACGCGCACTTCAACGAGGTGTTCCTCACCGACGTCTTCGTGCCCGACGAGATGTTGCT
CGGCGAACCGACCGGCGGCTGGAATCTGGCCATCGCCACGATGGCGCAGGAGCGGGTGGCCATCGGCAACTACGTGCACA
ACGACCGGGCGGTGGCCCTGCGCGCCCTGGCTCGCACGCCCGGACCCGACCAGGACGCGGCGCTGCGCGCGCTGGGCGAC
ATCGACGCCTACGCCGGATCGATCAAGGCGCTGGGTGTGCGCGAGACGCTGCGGCTGCTCGACGGTCAGCACGCCGGGCC
GGCATCGAGCATCGCGAAGGTCGCCACCGCGGCCATGCTGCGGCGCACGTTCGAGGCCACCCTTCAGTTGACCGGTGAGA
CCGCGCTGCTCGAGGACACCGATCCGCCTGTGGTGCAGCCGTACCTGCACCTGCCGGCCGAACTCGTCGGCGGCGGCACC
AGGGAGATCCAGCTCAACATCATCGCCCAGATGGTGCTCGGGCTACCTCGTGGATGA

Upstream 100 bases:

>100_bases
GCGAACTCGGATGGCGTCCGGCACCCACGACGGATGCCCTCGCCGAGGCCGCGGAGTTCTTCGTCGGCAACCGCCGCCGA
CCCGCCGAGGACCGCCGCTG

Downstream 100 bases:

>100_bases
CAGGGAGACACGGATGGGATTGCGTGGTGAGGCGGCGATCGTCGGCTATGTCGAGTTGCCGCCGGAGCGGCTGAACAAGG
CCGGCCCGGCACCGTTCCTC

Product: acyl-CoA dehydrogenase-like protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MPIALTPEQRALSDAVREFAARHAPVEKTRQAFDDLAAGNVPTWWDDLVSAGFHAVHLPERVGGQGGDLVDTACVVEAAA
QAMLPGPLLSTVATGSVALLADTVTSESLLRDLAGGTTAAVILPNDAALQAVSTPDGWEVSGVSPVVLGVGAARLLLAAA
HTEDGGLIWFVADADAAVTECERGTDLTTDVARVRFDGHQVASGARLTGIDTERARCAVAALTASASAGTIRWCVDAVTE
HLRTREQFGKPIGTFQALQHRAADLLISSELATAAAWDAVRSADEPAEQHVIAAATAVLMAAAPAPDLVLDTLTMFGAIG
FTWEHDLHLYWRRATALAASLGPATRWSRRLGELTRTTARDVTVRLGDADPEFRARVSDVLDRASTLPNDGPRPQGDYDQ
FATGPQRTAIAEAGLIAPHYPAPWGLDATPLQQLIIDEEFAARPALTRPSLGIAEWIVPSLISAAGQEVRDRLIPPTLRG
ETAWCQLFSEPSAGSDLASLTTRATRVDGGWKINGHKIWTSSAQHAHFGALLARTDPDARKHRGIGYFVVDMSSEGVEVQ
PIRQVTGDAHFNEVFLTDVFVPDEMLLGEPTGGWNLAIATMAQERVAIGNYVHNDRAVALRALARTPGPDQDAALRALGD
IDAYAGSIKALGVRETLRLLDGQHAGPASSIAKVATAAMLRRTFEATLQLTGETALLEDTDPPVVQPYLHLPAELVGGGT
REIQLNIIAQMVLGLPRG

Sequences:

>Translated_738_residues
MPIALTPEQRALSDAVREFAARHAPVEKTRQAFDDLAAGNVPTWWDDLVSAGFHAVHLPERVGGQGGDLVDTACVVEAAA
QAMLPGPLLSTVATGSVALLADTVTSESLLRDLAGGTTAAVILPNDAALQAVSTPDGWEVSGVSPVVLGVGAARLLLAAA
HTEDGGLIWFVADADAAVTECERGTDLTTDVARVRFDGHQVASGARLTGIDTERARCAVAALTASASAGTIRWCVDAVTE
HLRTREQFGKPIGTFQALQHRAADLLISSELATAAAWDAVRSADEPAEQHVIAAATAVLMAAAPAPDLVLDTLTMFGAIG
FTWEHDLHLYWRRATALAASLGPATRWSRRLGELTRTTARDVTVRLGDADPEFRARVSDVLDRASTLPNDGPRPQGDYDQ
FATGPQRTAIAEAGLIAPHYPAPWGLDATPLQQLIIDEEFAARPALTRPSLGIAEWIVPSLISAAGQEVRDRLIPPTLRG
ETAWCQLFSEPSAGSDLASLTTRATRVDGGWKINGHKIWTSSAQHAHFGALLARTDPDARKHRGIGYFVVDMSSEGVEVQ
PIRQVTGDAHFNEVFLTDVFVPDEMLLGEPTGGWNLAIATMAQERVAIGNYVHNDRAVALRALARTPGPDQDAALRALGD
IDAYAGSIKALGVRETLRLLDGQHAGPASSIAKVATAAMLRRTFEATLQLTGETALLEDTDPPVVQPYLHLPAELVGGGT
REIQLNIIAQMVLGLPRG
>Mature_737_residues
PIALTPEQRALSDAVREFAARHAPVEKTRQAFDDLAAGNVPTWWDDLVSAGFHAVHLPERVGGQGGDLVDTACVVEAAAQ
AMLPGPLLSTVATGSVALLADTVTSESLLRDLAGGTTAAVILPNDAALQAVSTPDGWEVSGVSPVVLGVGAARLLLAAAH
TEDGGLIWFVADADAAVTECERGTDLTTDVARVRFDGHQVASGARLTGIDTERARCAVAALTASASAGTIRWCVDAVTEH
LRTREQFGKPIGTFQALQHRAADLLISSELATAAAWDAVRSADEPAEQHVIAAATAVLMAAAPAPDLVLDTLTMFGAIGF
TWEHDLHLYWRRATALAASLGPATRWSRRLGELTRTTARDVTVRLGDADPEFRARVSDVLDRASTLPNDGPRPQGDYDQF
ATGPQRTAIAEAGLIAPHYPAPWGLDATPLQQLIIDEEFAARPALTRPSLGIAEWIVPSLISAAGQEVRDRLIPPTLRGE
TAWCQLFSEPSAGSDLASLTTRATRVDGGWKINGHKIWTSSAQHAHFGALLARTDPDARKHRGIGYFVVDMSSEGVEVQP
IRQVTGDAHFNEVFLTDVFVPDEMLLGEPTGGWNLAIATMAQERVAIGNYVHNDRAVALRALARTPGPDQDAALRALGDI
DAYAGSIKALGVRETLRLLDGQHAGPASSIAKVATAAMLRRTFEATLQLTGETALLEDTDPPVVQPYLHLPAELVGGGTR
EIQLNIIAQMVLGLPRG

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI4557233, Length=153, Percent_Identity=28.1045751633987, Blast_Score=68, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17506239, Length=360, Percent_Identity=24.7222222222222, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17570075, Length=271, Percent_Identity=25.4612546125461, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508101, Length=215, Percent_Identity=29.3023255813954, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32563615, Length=199, Percent_Identity=29.1457286432161, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17551932, Length=284, Percent_Identity=22.5352112676056, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356377, Length=150, Percent_Identity=30, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 78206; Mature: 78075

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIALTPEQRALSDAVREFAARHAPVEKTRQAFDDLAAGNVPTWWDDLVSAGFHAVHLPE
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECHH
RVGGQGGDLVDTACVVEAAAQAMLPGPLLSTVATGSVALLADTVTSESLLRDLAGGTTAA
HHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCEEE
VILPNDAALQAVSTPDGWEVSGVSPVVLGVGAARLLLAAAHTEDGGLIWFVADADAAVTE
EEECCHHHEEEECCCCCCEECCCCHHHHHHHHHHHHHHHEECCCCCEEEEEECCCHHHHH
CERGTDLTTDVARVRFDGHQVASGARLTGIDTERARCAVAALTASASAGTIRWCVDAVTE
HHCCCCCHHHHHHEEECCHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHH
HLRTREQFGKPIGTFQALQHRAADLLISSELATAAAWDAVRSADEPAEQHVIAAATAVLM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAAPAPDLVLDTLTMFGAIGFTWEHDLHLYWRRATALAASLGPATRWSRRLGELTRTTAR
HCCCCCHHHHHHHHHHHCCCCEEECCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
DVTVRLGDADPEFRARVSDVLDRASTLPNDGPRPQGDYDQFATGPQRTAIAEAGLIAPHY
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCC
PAPWGLDATPLQQLIIDEEFAARPALTRPSLGIAEWIVPSLISAAGQEVRDRLIPPTLRG
CCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ETAWCQLFSEPSAGSDLASLTTRATRVDGGWKINGHKIWTSSAQHAHFGALLARTDPDAR
CHHHHHEECCCCCCCHHHHHHHHHEECCCCEEECCEEEECCCCCCHHHHHEEECCCCHHH
KHRGIGYFVVDMSSEGVEVQPIRQVTGDAHFNEVFLTDVFVPDEMLLGEPTGGWNLAIAT
HHCCCEEEEEECCCCCCEEEHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCEEEEEH
MAQERVAIGNYVHNDRAVALRALARTPGPDQDAALRALGDIDAYAGSIKALGVRETLRLL
HHHHHHHHCCHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHCCCHHHHHHHH
DGQHAGPASSIAKVATAAMLRRTFEATLQLTGETALLEDTDPPVVQPYLHLPAELVGGGT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCCCHHHHCCHHHHCCCC
REIQLNIIAQMVLGLPRG
EEEHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PIALTPEQRALSDAVREFAARHAPVEKTRQAFDDLAAGNVPTWWDDLVSAGFHAVHLPE
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECHH
RVGGQGGDLVDTACVVEAAAQAMLPGPLLSTVATGSVALLADTVTSESLLRDLAGGTTAA
HHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCEEE
VILPNDAALQAVSTPDGWEVSGVSPVVLGVGAARLLLAAAHTEDGGLIWFVADADAAVTE
EEECCHHHEEEECCCCCCEECCCCHHHHHHHHHHHHHHHEECCCCCEEEEEECCCHHHHH
CERGTDLTTDVARVRFDGHQVASGARLTGIDTERARCAVAALTASASAGTIRWCVDAVTE
HHCCCCCHHHHHHEEECCHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHH
HLRTREQFGKPIGTFQALQHRAADLLISSELATAAAWDAVRSADEPAEQHVIAAATAVLM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAAPAPDLVLDTLTMFGAIGFTWEHDLHLYWRRATALAASLGPATRWSRRLGELTRTTAR
HCCCCCHHHHHHHHHHHCCCCEEECCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
DVTVRLGDADPEFRARVSDVLDRASTLPNDGPRPQGDYDQFATGPQRTAIAEAGLIAPHY
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCC
PAPWGLDATPLQQLIIDEEFAARPALTRPSLGIAEWIVPSLISAAGQEVRDRLIPPTLRG
CCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ETAWCQLFSEPSAGSDLASLTTRATRVDGGWKINGHKIWTSSAQHAHFGALLARTDPDAR
CHHHHHEECCCCCCCHHHHHHHHHEECCCCEEECCEEEECCCCCCHHHHHEEECCCCHHH
KHRGIGYFVVDMSSEGVEVQPIRQVTGDAHFNEVFLTDVFVPDEMLLGEPTGGWNLAIAT
HHCCCEEEEEECCCCCCEEEHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCEEEEEH
MAQERVAIGNYVHNDRAVALRALARTPGPDQDAALRALGDIDAYAGSIKALGVRETLRLL
HHHHHHHHCCHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHCCCHHHHHHHH
DGQHAGPASSIAKVATAAMLRRTFEATLQLTGETALLEDTDPPVVQPYLHLPAELVGGGT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCCCHHHHCCHHHHCCCC
REIQLNIIAQMVLGLPRG
EEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]