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The map label for this gene is pleD [H]

Identifier: 108763957

GI number: 108763957

Start: 6680774

End: 6681736

Strand: Reverse

Name: pleD [H]

Synonym: MXAN_5366

Alternate gene names: 108763957

Gene position: 6681736-6680774 (Counterclockwise)

Preceding gene: 108758836

Following gene: 108763090

Centisome position: 73.11

GC content: 69.89

Gene sequence:

>963_bases
GTGAAATCACCATTTTGCGGGGACACTGTGAACTCCAGGCCCTTCACCCTGCTGGTAGTGGACGACTCGCAGTCGACGTT
GCCTCGGTTGGCCCGTGCGCTGGGCCCGGAGGATTTCGCCCTCCGAATCACCGCCCACGGCCCGGAGGTGCCGAGGTTGG
CGCGGGAGGTATCGCTGGTGGTGCTCTGCCCGGGCGAGGACACCCAGGCCAGCGTGGGGTTGCTCGAACGCCTGATACCC
GAGGAAGGCCCGGTGGGGCCCCCCGTGGTGCTCGTGGCCCCACCCGAACCCAGGGCATTCTGGCTGGAGGCCCTGCGCCG
CGGCGCTGAGGTCGTCTTGGACCCATGGACGCCGGATGAACTGGTGGCCCGGGTGCATCGCGCCCTGGCCGCGCAGGCCC
GGATGGCGTCGCTCGCCGCCCAGGTGAGCGAGCTGCAGCGGCTGTCGTCGACGGACGGCCTCACGGGCCTCCACAACCAC
CGGCATTTCCAGGAGCGGCTGAGGGAGGAGTTCCGCCGTGCCCAGCGTTATGACGACTCCCTGTCGCTCATCCTGCTGGA
CTTGGATTACTTCAAGGAAATCAACGACAAGTACGGCCACTCCGCGGGCGACGGCGTGCTGCGCGAGGTGGCCGCCGCGC
TCACCCGGGGCGTGCGCGAGACGGACCTGGTGGCCCGCTATGGCGGCGAGGAGTTCGCGGTGCTGCTGCCCCGCACCCAC
CTCACCGGGGCGCTCACCGTCGCCGAGCGCGTCCGCCGCGAGCTGCGCGCCCTGCGGCTGGAGGTGTCGGACAGCCTGCA
AGTCACCGCGTCATTGGGGGTTTCCAGCTTCCCCCACCGCACCGTCCTCACCCCGGAGCAGCTGCTCCTGACGGCCGACG
AGGCCCTCTACCAGGCCAAACGGGACGGCAGGGATCGCATCTGCCTACATCCCCAGCTCCCGGTCGGTCCGCTCGGAACC
TAG

Upstream 100 bases:

>100_bases
GACCTCGGCGGGCGGACCTGCGAAGTTGGCGGCTCGCTAGCAGAGAATCCGGTTTTTATCCAATTGCAGGGGTCGCATAT
CGTCGCATGCCTATGATACC

Downstream 100 bases:

>100_bases
CCCGGGTCAAAGACCCGGGGTCTATGTTGACCCATTTGGTGGGGATGGGTCTAATTGGACCTGGCGTTTTTTCGCGCCAT
GGAAATGTCAAGGCCCGTGA

Product: response regulator/GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKSPFCGDTVNSRPFTLLVVDDSQSTLPRLARALGPEDFALRITAHGPEVPRLAREVSLVVLCPGEDTQASVGLLERLIP
EEGPVGPPVVLVAPPEPRAFWLEALRRGAEVVLDPWTPDELVARVHRALAAQARMASLAAQVSELQRLSSTDGLTGLHNH
RHFQERLREEFRRAQRYDDSLSLILLDLDYFKEINDKYGHSAGDGVLREVAAALTRGVRETDLVARYGGEEFAVLLPRTH
LTGALTVAERVRRELRALRLEVSDSLQVTASLGVSSFPHRTVLTPEQLLLTADEALYQAKRDGRDRICLHPQLPVGPLGT

Sequences:

>Translated_320_residues
MKSPFCGDTVNSRPFTLLVVDDSQSTLPRLARALGPEDFALRITAHGPEVPRLAREVSLVVLCPGEDTQASVGLLERLIP
EEGPVGPPVVLVAPPEPRAFWLEALRRGAEVVLDPWTPDELVARVHRALAAQARMASLAAQVSELQRLSSTDGLTGLHNH
RHFQERLREEFRRAQRYDDSLSLILLDLDYFKEINDKYGHSAGDGVLREVAAALTRGVRETDLVARYGGEEFAVLLPRTH
LTGALTVAERVRRELRALRLEVSDSLQVTASLGVSSFPHRTVLTPEQLLLTADEALYQAKRDGRDRICLHPQLPVGPLGT
>Mature_320_residues
MKSPFCGDTVNSRPFTLLVVDDSQSTLPRLARALGPEDFALRITAHGPEVPRLAREVSLVVLCPGEDTQASVGLLERLIP
EEGPVGPPVVLVAPPEPRAFWLEALRRGAEVVLDPWTPDELVARVHRALAAQARMASLAAQVSELQRLSSTDGLTGLHNH
RHFQERLREEFRRAQRYDDSLSLILLDLDYFKEINDKYGHSAGDGVLREVAAALTRGVRETDLVARYGGEEFAVLLPRTH
LTGALTVAERVRRELRALRLEVSDSLQVTASLGVSSFPHRTVLTPEQLLLTADEALYQAKRDGRDRICLHPQLPVGPLGT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=175, Percent_Identity=36.5714285714286, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=41.3173652694611, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=38.6075949367089, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=41.0714285714286, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=38.5093167701863, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787262, Length=198, Percent_Identity=29.2929292929293, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=32.7485380116959, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=31.9767441860465, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=34.9693251533742, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788085, Length=211, Percent_Identity=26.5402843601896, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87081977, Length=188, Percent_Identity=32.4468085106383, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=174, Percent_Identity=31.6091954022989, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=25.7668711656442, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=29.4797687861272, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35309; Mature: 35309

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPFCGDTVNSRPFTLLVVDDSQSTLPRLARALGPEDFALRITAHGPEVPRLAREVSLV
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCEEE
VLCPGEDTQASVGLLERLIPEEGPVGPPVVLVAPPEPRAFWLEALRRGAEVVLDPWTPDE
EEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCHHH
LVARVHRALAAQARMASLAAQVSELQRLSSTDGLTGLHNHRHFQERLREEFRRAQRYDDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
LSLILLDLDYFKEINDKYGHSAGDGVLREVAAALTRGVRETDLVARYGGEEFAVLLPRTH
CEEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCH
LTGALTVAERVRRELRALRLEVSDSLQVTASLGVSSFPHRTVLTPEQLLLTADEALYQAK
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RDGRDRICLHPQLPVGPLGT
HCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKSPFCGDTVNSRPFTLLVVDDSQSTLPRLARALGPEDFALRITAHGPEVPRLAREVSLV
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCEEE
VLCPGEDTQASVGLLERLIPEEGPVGPPVVLVAPPEPRAFWLEALRRGAEVVLDPWTPDE
EEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCHHH
LVARVHRALAAQARMASLAAQVSELQRLSSTDGLTGLHNHRHFQERLREEFRRAQRYDDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
LSLILLDLDYFKEINDKYGHSAGDGVLREVAAALTRGVRETDLVARYGGEEFAVLLPRTH
CEEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCH
LTGALTVAERVRRELRALRLEVSDSLQVTASLGVSSFPHRTVLTPEQLLLTADEALYQAK
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RDGRDRICLHPQLPVGPLGT
HCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA