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The map label for this gene is aes [H]

Identifier: 108763920

GI number: 108763920

Start: 798169

End: 799119

Strand: Direct

Name: aes [H]

Synonym: MXAN_0700

Alternate gene names: 108763920

Gene position: 798169-799119 (Clockwise)

Preceding gene: 108762394

Following gene: 108760293

Centisome position: 8.73

GC content: 67.09

Gene sequence:

>951_bases
ATGAACCTGGACCCTTCCACGCAGAAGTTCGTCTCGGACCTGGCGGCCTCGAACAGCCCGCCGCTGTACACCCTGACGCC
AGAGCAGGCACGGGAAGTCCTCCTCGAGGCGCAGTCGATTCCAGTGCCCATGCCAGACGCGGACGTGGAGGAGCGCAAGC
TGCCCGTGGGGCCCAGGGGCTCCGTCCGGACGCTGCTCTTCCGGCCGAAGGGGAGCAAGGAGCGCCTGCCGGTGCTGATG
TTCGTCCACGGCGCGGGCTGGGTGATGGGAGATGCCAGGACGCACGAGCGGCTGGTGCGGGAGCTGGTCAAGGGGGCGAA
TGTCGCAGCGGTGTTCGTGGACTATGGCCGCTCGCCTGAGAACAAGTTCCCCGTGGCCATCGAAGAGGCCTATGCCGCGA
CGAAGTACGTGGCGGAGCACCCGGACGAATTCAACGTGGATGCCCGCCGCATGGCGCTGGTGGGAGACAGCGTCGGAGGG
AACATGGCGACCGTCGTCAGTATGCTCGCGAAGGAGCGCGGGGGGCCGTCCATCCGCTTCCAGGTGCTCTTCTATCCCGT
CACCGACGCCAACTTCGACAACGGCTCCTACCAGGAGTTCGCGGAGGGGCCGTGGCTGACGCGCAAGGCGATGAAGTGGT
TCTGGGATGCCTATCTTCCGGAGGCCGGCAAGCGCGTGGACCCACATGTTTCCCCGCTGCGGGCGTCACTGGACCAGCTC
AACGGCTTGCCCCCCGCGCTGGTCATCACCGACGAGAACGACGTGCTGCGTGACGAGGGCGAGGCCTACGCGGCGAAGCT
CTCCGAGGCGGGTGTCAACGTCACTCAGGTCCGCTTCCTGGGGACGCATCACGACTTCGTCATGCTCAACGCGCTGGCGC
AGACGCCGGCGGCGCGAGGCGCCATCGCGCTGACCACCACCAAGCTGCGCGAGTGGCTGCACGCGAACTGA

Upstream 100 bases:

>100_bases
CCGCGTCGGGTCCTTGCCCGCATGGTTGCTGACGCTCGCGCCCGGGTTGGCACCTTTCCGGCGGCGTCCGTCCCGGGCGC
CGGACCAAGGAGCAGCGCGC

Downstream 100 bases:

>100_bases
GCCAGCCTTTCATTTCTGAGAGCCGGCCGGGGAGACTGGCGCGCGCATGACGGGCGCCGGCTCTCCAAGGCAATGGCTGC
ATGACGACTGCCTCTCAATC

Product: putative lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNLDPSTQKFVSDLAASNSPPLYTLTPEQAREVLLEAQSIPVPMPDADVEERKLPVGPRGSVRTLLFRPKGSKERLPVLM
FVHGAGWVMGDARTHERLVRELVKGANVAAVFVDYGRSPENKFPVAIEEAYAATKYVAEHPDEFNVDARRMALVGDSVGG
NMATVVSMLAKERGGPSIRFQVLFYPVTDANFDNGSYQEFAEGPWLTRKAMKWFWDAYLPEAGKRVDPHVSPLRASLDQL
NGLPPALVITDENDVLRDEGEAYAAKLSEAGVNVTQVRFLGTHHDFVMLNALAQTPAARGAIALTTTKLREWLHAN

Sequences:

>Translated_316_residues
MNLDPSTQKFVSDLAASNSPPLYTLTPEQAREVLLEAQSIPVPMPDADVEERKLPVGPRGSVRTLLFRPKGSKERLPVLM
FVHGAGWVMGDARTHERLVRELVKGANVAAVFVDYGRSPENKFPVAIEEAYAATKYVAEHPDEFNVDARRMALVGDSVGG
NMATVVSMLAKERGGPSIRFQVLFYPVTDANFDNGSYQEFAEGPWLTRKAMKWFWDAYLPEAGKRVDPHVSPLRASLDQL
NGLPPALVITDENDVLRDEGEAYAAKLSEAGVNVTQVRFLGTHHDFVMLNALAQTPAARGAIALTTTKLREWLHAN
>Mature_316_residues
MNLDPSTQKFVSDLAASNSPPLYTLTPEQAREVLLEAQSIPVPMPDADVEERKLPVGPRGSVRTLLFRPKGSKERLPVLM
FVHGAGWVMGDARTHERLVRELVKGANVAAVFVDYGRSPENKFPVAIEEAYAATKYVAEHPDEFNVDARRMALVGDSVGG
NMATVVSMLAKERGGPSIRFQVLFYPVTDANFDNGSYQEFAEGPWLTRKAMKWFWDAYLPEAGKRVDPHVSPLRASLDQL
NGLPPALVITDENDVLRDEGEAYAAKLSEAGVNVTQVRFLGTHHDFVMLNALAQTPAARGAIALTTTKLREWLHAN

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=274, Percent_Identity=27.7372262773723, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI206597554, Length=273, Percent_Identity=27.1062271062271, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI61966717, Length=290, Percent_Identity=25.5172413793103, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI68051721, Length=277, Percent_Identity=25.6317689530686, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI68299767, Length=273, Percent_Identity=26.007326007326, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI226423947, Length=285, Percent_Identity=24.5614035087719, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786682, Length=240, Percent_Identity=25, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=252, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI72001146, Length=125, Percent_Identity=34.4, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=125, Percent_Identity=32, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=139, Percent_Identity=29.4964028776978, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34808; Mature: 34808

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDPSTQKFVSDLAASNSPPLYTLTPEQAREVLLEAQSIPVPMPDADVEERKLPVGPRG
CCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCC
SVRTLLFRPKGSKERLPVLMFVHGAGWVMGDARTHERLVRELVKGANVAAVFVDYGRSPE
CEEEEEECCCCCCCCCCEEEEEECCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCC
NKFPVAIEEAYAATKYVAEHPDEFNVDARRMALVGDSVGGNMATVVSMLAKERGGPSIRF
CCCCEEHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHHHHCCCCCEEE
QVLFYPVTDANFDNGSYQEFAEGPWLTRKAMKWFWDAYLPEAGKRVDPHVSPLRASLDQL
EEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
NGLPPALVITDENDVLRDEGEAYAAKLSEAGVNVTQVRFLGTHHDFVMLNALAQTPAARG
CCCCCEEEEECCCCHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCC
AIALTTTKLREWLHAN
EEEEEHHHHHHHHCCC
>Mature Secondary Structure
MNLDPSTQKFVSDLAASNSPPLYTLTPEQAREVLLEAQSIPVPMPDADVEERKLPVGPRG
CCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCC
SVRTLLFRPKGSKERLPVLMFVHGAGWVMGDARTHERLVRELVKGANVAAVFVDYGRSPE
CEEEEEECCCCCCCCCCEEEEEECCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCC
NKFPVAIEEAYAATKYVAEHPDEFNVDARRMALVGDSVGGNMATVVSMLAKERGGPSIRF
CCCCEEHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHHHHCCCCCEEE
QVLFYPVTDANFDNGSYQEFAEGPWLTRKAMKWFWDAYLPEAGKRVDPHVSPLRASLDQL
EEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
NGLPPALVITDENDVLRDEGEAYAAKLSEAGVNVTQVRFLGTHHDFVMLNALAQTPAARG
CCCCCEEEEECCCCHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCC
AIALTTTKLREWLHAN
EEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA