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The map label for this gene is paaF [C]

Identifier: 108763919

GI number: 108763919

Start: 6898475

End: 6899254

Strand: Direct

Name: paaF [C]

Synonym: MXAN_5549

Alternate gene names: 108763919

Gene position: 6898475-6899254 (Clockwise)

Preceding gene: 108757404

Following gene: 108758863

Centisome position: 75.48

GC content: 67.56

Gene sequence:

>780_bases
ATGAGCAACTTCATCACGGAAGAGACCGAAGGGTTCGTGCGGAAGATTGGACTGAACCGCCCTGAGAAGCGGAACGCGAT
GAACATCGCGCTCATCGAGCAACTCTCGGGTGCGCTGGGGCGGGCCGAGGCCGACGAGAACATTCGCGTGTGCGTGCTCT
TCGCTCACGGCACCGTGTTCACGGCGGGGCTGGACCTCATGGATGTGTTTCCCAGGCTGGGTGACGCCCAGACGCTGTTC
AGCGCGGCGGGCATCGACCCGTGGGCCACGCATGGCCCCGCGAGGACGAAGCCGCTCATCATCGCGGTGCATGGCAAGTG
CCTCACGCTCGGTATCGAACTGATGCTCGCTGCCGACATCACGGTGGCTTCCGAGGACGCCACCTTCGAGCAGATTGAAA
TCGACCGCGGCATCTTCCCCTTCGGCGGGGGCACGGCCCGCTGGGTGCGGACCATGGGTTGGGGCAACGCGATGCAGTAC
CTGCTGACGGGTGATGCGCTCGACGTGCGTGAGGCGCATCGGTTGGGGCTCGTGCAGCGTGTCGTCTCGCGCGAGGCCCT
GATGGAGACGGCGATGGGCCTCGCGGCCCGCATCGCGTCGAAGCCGCCCCTCGCCATCAAGGCGACCATCGAGAGTGCGC
GGACGGCCGTCCTCGAAGGGGAGCGCGCCGCGGCGGCGAAGCTCCTGCCGGCCATCCAGCAGCTCGCGACGACCGAGGAT
GTGCAGGAGGCGCTCTCCGCCCACTTCGAACGCCGGCCCGCGACCTTCCGCGGCCGATGA

Upstream 100 bases:

>100_bases
ACTTCGGCTGGTTCCGCTCCAGCATGCCCGAGGTCGTCTGGGTGGAGGCGGCGGATTGGCTCGAACAGACGAAGTGACTT
CACCACCCAACGAGGGACTC

Downstream 100 bases:

>100_bases
CAGGCTCAGGAGGCCGGGGCATGCCAGCGCATCCAGAGCCCCATCACGGGGAGCCCACCTCGCTCCGCGCGTCCGACGGA
TTCTCGCTCGGCGCGAGCCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSNFITEETEGFVRKIGLNRPEKRNAMNIALIEQLSGALGRAEADENIRVCVLFAHGTVFTAGLDLMDVFPRLGDAQTLF
SAAGIDPWATHGPARTKPLIIAVHGKCLTLGIELMLAADITVASEDATFEQIEIDRGIFPFGGGTARWVRTMGWGNAMQY
LLTGDALDVREAHRLGLVQRVVSREALMETAMGLAARIASKPPLAIKATIESARTAVLEGERAAAAKLLPAIQQLATTED
VQEALSAHFERRPATFRGR

Sequences:

>Translated_259_residues
MSNFITEETEGFVRKIGLNRPEKRNAMNIALIEQLSGALGRAEADENIRVCVLFAHGTVFTAGLDLMDVFPRLGDAQTLF
SAAGIDPWATHGPARTKPLIIAVHGKCLTLGIELMLAADITVASEDATFEQIEIDRGIFPFGGGTARWVRTMGWGNAMQY
LLTGDALDVREAHRLGLVQRVVSREALMETAMGLAARIASKPPLAIKATIESARTAVLEGERAAAAKLLPAIQQLATTED
VQEALSAHFERRPATFRGR
>Mature_258_residues
SNFITEETEGFVRKIGLNRPEKRNAMNIALIEQLSGALGRAEADENIRVCVLFAHGTVFTAGLDLMDVFPRLGDAQTLFS
AAGIDPWATHGPARTKPLIIAVHGKCLTLGIELMLAADITVASEDATFEQIEIDRGIFPFGGGTARWVRTMGWGNAMQYL
LTGDALDVREAHRLGLVQRVVSREALMETAMGLAARIASKPPLAIKATIESARTAVLEGERAAAAKLLPAIQQLATTEDV
QEALSAHFERRPATFRGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=32.9457364341085, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI70995211, Length=263, Percent_Identity=30.4182509505703, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI31542718, Length=191, Percent_Identity=28.2722513089005, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI221136756, Length=210, Percent_Identity=27.1428571428571, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136753, Length=210, Percent_Identity=27.1428571428571, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221307494, Length=210, Percent_Identity=27.1428571428571, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI4757966, Length=225, Percent_Identity=25.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI51538581, Length=225, Percent_Identity=25.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI25453481, Length=225, Percent_Identity=25.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI51538573, Length=225, Percent_Identity=25.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4502327, Length=273, Percent_Identity=28.9377289377289, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757968, Length=182, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI49355787, Length=182, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI260274832, Length=247, Percent_Identity=25.5060728744939, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45643119, Length=247, Percent_Identity=25.5060728744939, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI260275230, Length=247, Percent_Identity=25.5060728744939, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=35.9073359073359, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1787660, Length=270, Percent_Identity=32.962962962963, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI221142681, Length=274, Percent_Identity=30.6569343065693, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=26.1538461538462, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=30.5263157894737, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=25.6916996047431, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=237, Percent_Identity=36.2869198312236, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=30.6772908366534, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=219, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=232, Percent_Identity=29.7413793103448, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=27.734375, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=204, Percent_Identity=29.9019607843137, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=204, Percent_Identity=24.0196078431373, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=26.3440860215054, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=32.1705426356589, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=32.1705426356589, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI19920382, Length=259, Percent_Identity=30.1158301158301, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24650670, Length=221, Percent_Identity=33.4841628959276, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357171, Length=215, Percent_Identity=29.3023255813954, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=29.1338582677165, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28019; Mature: 27887

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFITEETEGFVRKIGLNRPEKRNAMNIALIEQLSGALGRAEADENIRVCVLFAHGTVF
CCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEE
TAGLDLMDVFPRLGDAQTLFSAAGIDPWATHGPARTKPLIIAVHGKCLTLGIELMLAADI
ECCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHEEECE
TVASEDATFEQIEIDRGIFPFGGGTARWVRTMGWGNAMQYLLTGDALDVREAHRLGLVQR
EEECCCCCHHHEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHH
VVSREALMETAMGLAARIASKPPLAIKATIESARTAVLEGERAAAAKLLPAIQQLATTED
HHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VQEALSAHFERRPATFRGR
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SNFITEETEGFVRKIGLNRPEKRNAMNIALIEQLSGALGRAEADENIRVCVLFAHGTVF
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEE
TAGLDLMDVFPRLGDAQTLFSAAGIDPWATHGPARTKPLIIAVHGKCLTLGIELMLAADI
ECCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHEEECE
TVASEDATFEQIEIDRGIFPFGGGTARWVRTMGWGNAMQYLLTGDALDVREAHRLGLVQR
EEECCCCCHHHEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHH
VVSREALMETAMGLAARIASKPPLAIKATIESARTAVLEGERAAAAKLLPAIQQLATTED
HHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VQEALSAHFERRPATFRGR
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA