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The map label for this gene is nylA [H]

Identifier: 108763797

GI number: 108763797

Start: 3323279

End: 3324733

Strand: Reverse

Name: nylA [H]

Synonym: MXAN_2852

Alternate gene names: 108763797

Gene position: 3324733-3323279 (Counterclockwise)

Preceding gene: 108761029

Following gene: 108758370

Centisome position: 36.38

GC content: 72.03

Gene sequence:

>1455_bases
ATGAACCTGCATGACTACAGCCGCTTTGACGCAGTGGGATTGGCGGAGCTGGTACGTCGGAAGGAAGTCACGCCCGAAGA
GCTGCTCCGGGTGGCGGTGGAGGCCATCCACGCCGTCAACCCGGCACTGAACGCCGTCATCGACACGCGAGAGGGCGACG
CCAGCGAGGCGCTGAAGCAAGGCATTCCCGAGGGCCCCTTCCGAGGCGTTCCCTTCCTCATCAAGGACATTGGCCTGCAC
GCCGCGGGTGTGCCCACCGACATGGGCAGCCGGATGGCCCAGGGGACGGTGTTCCCCCATGACAGCGCGTTGATGGCGCG
GTACCGGCGCGCGGGCCTGGTGCTGCTGGGCCGGACGAACGCACCGGAGTTCGGCAACAACGCCACCACGGAGCCGGTGC
TGCACGGCCCCACGCGCAATCCGTGGGACGTGAAGCGCAGCCCGGGCGGCTCCAGCGGCGGCTCGGCGGCGGCGGTGGCG
GCGGGCATCGTCCCGGTGGCCCACGGCAACGATGGTGGGGGCTCGCTGCGCATCCCCGCGGCGCTTTGCGGCGTCTTCGG
GCTGAAGCCCACGCGTGGCCGCAACTCATTGGGGCCGGACGCGGGTGACTTCATCTTCGGCATGGGCATCGAGCACGTGC
TGTCCCGCAGCGTGCGCGACAGCGCGGCGATGCTGGACGCCACCCAGGGGCCGGAGGTGGGCGACCCGTACTTCGCCCCG
CCGCCGCAGCGCCCATACCTGGAGGAAGTGGGCCGCGCGCCGGGCCGGCTGCGCATCGCGCTGATGACGGAAGCCCCCAT
GGGCTCACCGGCGAGCGCCGAATGTGTGGAGGCCGCTCGAGCGGCGGCGCGCCTGTGTACGGAGCTGGGCCATGACGTCG
TCGAGGCCGCGCCCGAGCACGACGGCCAACTGCTGCACGAGGCCGTCTGCACCGTCTGGTCGGCGACCATGGCGTCCCTG
GTGGGCATGCTGGGCCAGTTCTCCGGCCGCGACCCGGGACCGGAGCTGCTGGAGGCCACCACCTGGGCGGCGGTGCGCCA
CGGCCGCGAGCTGAAGGCCACGGACCTGCAACGGGCGCTCGGGGTGTTCAACCTCGTGTCACGTCAGGTGGGCGCCTTCT
TCCAGCAGTATGACGTACTGCTGTCGCCCACGGTGGCGGCGCCGCCCTTCCCGCTGGGCCTGCTGGACGCCAACGCACCC
CGGACGGCCCGCGAGTGGTACGACCACGCGTTCGGCCACTGCCCCTTTACCGCGCTCTTCAACGTCACCGGGCAGCCCGC
CATGTCCGTGCCGCTGCACTGGAGCGCGGAGGGGCTGCCCATCGGCGTCCAGTTCGCCGGCCGGTATGCCGCCGAGGCCA
CGCTGTTCCAACTGGCGGGCCAGCTCGAGCAGGCGCGGCCCTGGGCCCAGCGGACGCCACCGGTGCACGTCACCCGGCCC
GCGGAGTCGCCGTGA

Upstream 100 bases:

>100_bases
GGCGACGGGCTGAGGTGCCTGGAGCGCGACCCGGCAATCAAGAATATCCTGCCTACCGCGACCGATCCGCCGCACGTCCG
CCGGCGCCAGTGAGGGATTC

Downstream 100 bases:

>100_bases
GGCGCGCGGTGGGCCCCTGAAGAAACGCGGAGGCCCACCGCGAACCCGCCAGGTCAGTCGTAGCGGCTGGTGAGCTTCAG
CGTCCACGAGCGCAGCGTGC

Product: amidase family protein

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNLHDYSRFDAVGLAELVRRKEVTPEELLRVAVEAIHAVNPALNAVIDTREGDASEALKQGIPEGPFRGVPFLIKDIGLH
AAGVPTDMGSRMAQGTVFPHDSALMARYRRAGLVLLGRTNAPEFGNNATTEPVLHGPTRNPWDVKRSPGGSSGGSAAAVA
AGIVPVAHGNDGGGSLRIPAALCGVFGLKPTRGRNSLGPDAGDFIFGMGIEHVLSRSVRDSAAMLDATQGPEVGDPYFAP
PPQRPYLEEVGRAPGRLRIALMTEAPMGSPASAECVEAARAAARLCTELGHDVVEAAPEHDGQLLHEAVCTVWSATMASL
VGMLGQFSGRDPGPELLEATTWAAVRHGRELKATDLQRALGVFNLVSRQVGAFFQQYDVLLSPTVAAPPFPLGLLDANAP
RTAREWYDHAFGHCPFTALFNVTGQPAMSVPLHWSAEGLPIGVQFAGRYAAEATLFQLAGQLEQARPWAQRTPPVHVTRP
AESP

Sequences:

>Translated_484_residues
MNLHDYSRFDAVGLAELVRRKEVTPEELLRVAVEAIHAVNPALNAVIDTREGDASEALKQGIPEGPFRGVPFLIKDIGLH
AAGVPTDMGSRMAQGTVFPHDSALMARYRRAGLVLLGRTNAPEFGNNATTEPVLHGPTRNPWDVKRSPGGSSGGSAAAVA
AGIVPVAHGNDGGGSLRIPAALCGVFGLKPTRGRNSLGPDAGDFIFGMGIEHVLSRSVRDSAAMLDATQGPEVGDPYFAP
PPQRPYLEEVGRAPGRLRIALMTEAPMGSPASAECVEAARAAARLCTELGHDVVEAAPEHDGQLLHEAVCTVWSATMASL
VGMLGQFSGRDPGPELLEATTWAAVRHGRELKATDLQRALGVFNLVSRQVGAFFQQYDVLLSPTVAAPPFPLGLLDANAP
RTAREWYDHAFGHCPFTALFNVTGQPAMSVPLHWSAEGLPIGVQFAGRYAAEATLFQLAGQLEQARPWAQRTPPVHVTRP
AESP
>Mature_484_residues
MNLHDYSRFDAVGLAELVRRKEVTPEELLRVAVEAIHAVNPALNAVIDTREGDASEALKQGIPEGPFRGVPFLIKDIGLH
AAGVPTDMGSRMAQGTVFPHDSALMARYRRAGLVLLGRTNAPEFGNNATTEPVLHGPTRNPWDVKRSPGGSSGGSAAAVA
AGIVPVAHGNDGGGSLRIPAALCGVFGLKPTRGRNSLGPDAGDFIFGMGIEHVLSRSVRDSAAMLDATQGPEVGDPYFAP
PPQRPYLEEVGRAPGRLRIALMTEAPMGSPASAECVEAARAAARLCTELGHDVVEAAPEHDGQLLHEAVCTVWSATMASL
VGMLGQFSGRDPGPELLEATTWAAVRHGRELKATDLQRALGVFNLVSRQVGAFFQQYDVLLSPTVAAPPFPLGLLDANAP
RTAREWYDHAFGHCPFTALFNVTGQPAMSVPLHWSAEGLPIGVQFAGRYAAEATLFQLAGQLEQARPWAQRTPPVHVTRP
AESP

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=27.2545090180361, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI195972892, Length=240, Percent_Identity=29.1666666666667, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI166795287, Length=443, Percent_Identity=25.9593679458239, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=25.8130081300813, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=32.0855614973262, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=203, Percent_Identity=31.5270935960591, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=205, Percent_Identity=33.1707317073171, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=201, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=201, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=296, Percent_Identity=27.7027027027027, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=473, Percent_Identity=23.6786469344609, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=411, Percent_Identity=25.5474452554745, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648113, Length=449, Percent_Identity=32.0712694877506, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356731, Length=496, Percent_Identity=25, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=25.4509018036072, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078093, Length=519, Percent_Identity=26.7822736030828, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI45550774, Length=195, Percent_Identity=32.3076923076923, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648435, Length=195, Percent_Identity=32.3076923076923, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648437, Length=195, Percent_Identity=32.3076923076923, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652985, Length=204, Percent_Identity=33.8235294117647, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922090, Length=204, Percent_Identity=33.8235294117647, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652981, Length=204, Percent_Identity=33.8235294117647, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652983, Length=204, Percent_Identity=33.8235294117647, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648441, Length=126, Percent_Identity=34.9206349206349, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648439, Length=126, Percent_Identity=34.9206349206349, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51318; Mature: 51318

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHDYSRFDAVGLAELVRRKEVTPEELLRVAVEAIHAVNPALNAVIDTREGDASEALKQ
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHEECCCCCHHHHHHC
GIPEGPFRGVPFLIKDIGLHAAGVPTDMGSRMAQGTVFPHDSALMARYRRAGLVLLGRTN
CCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECCC
APEFGNNATTEPVLHGPTRNPWDVKRSPGGSSGGSAAAVAAGIVPVAHGNDGGGSLRIPA
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCCCEEHHH
ALCGVFGLKPTRGRNSLGPDAGDFIFGMGIEHVLSRSVRDSAAMLDATQGPEVGDPYFAP
HHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PPQRPYLEEVGRAPGRLRIALMTEAPMGSPASAECVEAARAAARLCTELGHDVVEAAPEH
CCCCCHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
DGQLLHEAVCTVWSATMASLVGMLGQFSGRDPGPELLEATTWAAVRHGRELKATDLQRAL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
GVFNLVSRQVGAFFQQYDVLLSPTVAAPPFPLGLLDANAPRTAREWYDHAFGHCPFTALF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHH
NVTGQPAMSVPLHWSAEGLPIGVQFAGRYAAEATLFQLAGQLEQARPWAQRTPPVHVTRP
CCCCCCCEECCCEECCCCCEEEHEECCHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECC
AESP
CCCC
>Mature Secondary Structure
MNLHDYSRFDAVGLAELVRRKEVTPEELLRVAVEAIHAVNPALNAVIDTREGDASEALKQ
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHEECCCCCHHHHHHC
GIPEGPFRGVPFLIKDIGLHAAGVPTDMGSRMAQGTVFPHDSALMARYRRAGLVLLGRTN
CCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECCC
APEFGNNATTEPVLHGPTRNPWDVKRSPGGSSGGSAAAVAAGIVPVAHGNDGGGSLRIPA
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCCCEEHHH
ALCGVFGLKPTRGRNSLGPDAGDFIFGMGIEHVLSRSVRDSAAMLDATQGPEVGDPYFAP
HHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PPQRPYLEEVGRAPGRLRIALMTEAPMGSPASAECVEAARAAARLCTELGHDVVEAAPEH
CCCCCHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
DGQLLHEAVCTVWSATMASLVGMLGQFSGRDPGPELLEATTWAAVRHGRELKATDLQRAL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
GVFNLVSRQVGAFFQQYDVLLSPTVAAPPFPLGLLDANAPRTAREWYDHAFGHCPFTALF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHH
NVTGQPAMSVPLHWSAEGLPIGVQFAGRYAAEATLFQLAGQLEQARPWAQRTPPVHVTRP
CCCCCCCEECCCEECCCCCEEEHEECCHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECC
AESP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]