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The map label for this gene is pksF [H]

Identifier: 108763709

GI number: 108763709

Start: 4799268

End: 4800521

Strand: Reverse

Name: pksF [H]

Synonym: MXAN_3941

Alternate gene names: 108763709

Gene position: 4800521-4799268 (Counterclockwise)

Preceding gene: 108762541

Following gene: 108757680

Centisome position: 52.52

GC content: 69.78

Gene sequence:

>1254_bases
ATGTCCACCTCACCTGTGCAGGAGCTGGTTGTCTCGGGGTTCGGGGTCACCTCCGCCATTGGCCAGGGGGCCGCGTCCTT
CACCTCGGCGCTGCTGGAGGGCGCGGCACGGTTCCGGGTGATGGAGCGGCCGGGCCGTCAGCATCAGGCCAACGGGCAGA
CGACGGCCCACCTGGGGGCGGAAATCGCCTCGCTGGCCGTGCCCGAAGGCGTCACCCCACAACTGTGGCGCTCGGCCACG
TTTTCGGGGCAGGCCGCACTGGTGACCGTCCACGAGGCCTGGAACGCGGCGCGCCTCCAGGCCGTCCCCGGACACCGGAT
TGGATTGGTGGTGGGGGGCACCAACGTGCAGCAGCGCGACCTGGTGCTGATGCAAGACGCCTATCGCGAGCGGGTGCCCT
TTCTGCGGGCGGCCTACGGGTCGACCTTCATGGACACCGACCTCGTGGGCCTCTGCACGCAGCAGTTCGCCATCCACGGG
ATGTCCTTCACGGTGGGAGGCGCATCGGCCAGTGGCCTGCTGGCGGTCATCCAGGCCGCGGAGGCGGTGCTCTCAAGAAG
GGTGGACGTTTGCATCGCCGTGGGGGCGCTGATGGACGTCTCCTACTGGGAATGCCAGGGCCTGCGGGCCATGGGCGCGA
TGGGCACCGACCGGTTCGCGCGGGAGCCGGAGCGTGCCTGCCGGCCCTTCGACCGGGAGAGTGATGGCTTCATCTTTGGA
GAGGCGTGCGGCGCCGTGGTGGTTGAGTCTGCGGAGCACGCTCGGCGACGCGGGGTGACTCCTCGCGGCATCCTGTCGGG
CTGGGCCATGCAGTTGGACGCGAGCCGCGGCCCGTTGTCGTCCATCGAAGGGGAGTCGCAGGTGATTGGGGCTGCGCTGC
GGCACGCGGACCTCGCGCCGGAGCGGGTGGACTACGTGAATCCTCACGGCAGCGGTTCGCGTCAGGGGGATGCCATCGAG
CTGGGGGCCTTGAAGGCGTGCGGCCTGACGCACGCCCGGGTCAACACCACGAAGTCCATCACCGGGCATGGCCTGTCCTC
GGCGGGTGCCGTGGGGCTCATCGCCACGCTGGTCCAGTTGGAGCAGGGCCGGCTGCACCCGTCCTTGAACCTGGTGGACC
CGATTGATTCATCGTTCCGCTGGGTGGGGGCCACCGCGGAGGCCCAGTCCCTCCAGAACGCGCTGGTGCTCGCCTACGGC
TTCGGCGGCATCAACACCGCTGTCGCCGTGCGCCGGAGCGCCACGGAGAGCTGA

Upstream 100 bases:

>100_bases
CCGGCCACCGCCGCGCCCGCGAAGTGGGCGAATGCCTGAGCCCTCCGGAGCGTCGTTGAAATCCTCGGCCGGTGGGCCGT
CCGGCTGCTGAGACCACTGA

Downstream 100 bases:

>100_bases
CACGCCCATGCAAGCCGCTTCCCCTCCGCACCGCGACTACCAGACGCTCCGGGTCCGCTTCGAGGCGCAGACCTGTTTTC
TCCAGCTCCACCGGCCGGAT

Product: polyketide beta-ketoacyl:acyl carrier protein synthase

Products: NA

Alternate protein names: Malonyl acyl-carrier-protein decarboxylase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSTSPVQELVVSGFGVTSAIGQGAASFTSALLEGAARFRVMERPGRQHQANGQTTAHLGAEIASLAVPEGVTPQLWRSAT
FSGQAALVTVHEAWNAARLQAVPGHRIGLVVGGTNVQQRDLVLMQDAYRERVPFLRAAYGSTFMDTDLVGLCTQQFAIHG
MSFTVGGASASGLLAVIQAAEAVLSRRVDVCIAVGALMDVSYWECQGLRAMGAMGTDRFAREPERACRPFDRESDGFIFG
EACGAVVVESAEHARRRGVTPRGILSGWAMQLDASRGPLSSIEGESQVIGAALRHADLAPERVDYVNPHGSGSRQGDAIE
LGALKACGLTHARVNTTKSITGHGLSSAGAVGLIATLVQLEQGRLHPSLNLVDPIDSSFRWVGATAEAQSLQNALVLAYG
FGGINTAVAVRRSATES

Sequences:

>Translated_417_residues
MSTSPVQELVVSGFGVTSAIGQGAASFTSALLEGAARFRVMERPGRQHQANGQTTAHLGAEIASLAVPEGVTPQLWRSAT
FSGQAALVTVHEAWNAARLQAVPGHRIGLVVGGTNVQQRDLVLMQDAYRERVPFLRAAYGSTFMDTDLVGLCTQQFAIHG
MSFTVGGASASGLLAVIQAAEAVLSRRVDVCIAVGALMDVSYWECQGLRAMGAMGTDRFAREPERACRPFDRESDGFIFG
EACGAVVVESAEHARRRGVTPRGILSGWAMQLDASRGPLSSIEGESQVIGAALRHADLAPERVDYVNPHGSGSRQGDAIE
LGALKACGLTHARVNTTKSITGHGLSSAGAVGLIATLVQLEQGRLHPSLNLVDPIDSSFRWVGATAEAQSLQNALVLAYG
FGGINTAVAVRRSATES
>Mature_416_residues
STSPVQELVVSGFGVTSAIGQGAASFTSALLEGAARFRVMERPGRQHQANGQTTAHLGAEIASLAVPEGVTPQLWRSATF
SGQAALVTVHEAWNAARLQAVPGHRIGLVVGGTNVQQRDLVLMQDAYRERVPFLRAAYGSTFMDTDLVGLCTQQFAIHGM
SFTVGGASASGLLAVIQAAEAVLSRRVDVCIAVGALMDVSYWECQGLRAMGAMGTDRFAREPERACRPFDRESDGFIFGE
ACGAVVVESAEHARRRGVTPRGILSGWAMQLDASRGPLSSIEGESQVIGAALRHADLAPERVDYVNPHGSGSRQGDAIEL
GALKACGLTHARVNTTKSITGHGLSSAGAVGLIATLVQLEQGRLHPSLNLVDPIDSSFRWVGATAEAQSLQNALVLAYGF
GGINTAVAVRRSATES

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It decarboxylates selectively the malonyl group attached on the acyl-carrier-protein AcpK (Mal-AcpK) [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=309, Percent_Identity=29.126213592233, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI41872631, Length=272, Percent_Identity=26.8382352941176, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI223972627, Length=136, Percent_Identity=33.8235294117647, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788663, Length=421, Percent_Identity=26.8408551068884, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787337, Length=420, Percent_Identity=25.7142857142857, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17550940, Length=262, Percent_Identity=25.5725190839695, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI212642053, Length=274, Percent_Identity=25.9124087591241, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71983541, Length=224, Percent_Identity=25.8928571428571, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71983535, Length=224, Percent_Identity=25.8928571428571, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320904, Length=315, Percent_Identity=26.3492063492064, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644341, Length=298, Percent_Identity=26.510067114094, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 24024 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 30,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: =4.1.1.87 [H]

Molecular weight: Translated: 43772; Mature: 43641

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSPVQELVVSGFGVTSAIGQGAASFTSALLEGAARFRVMERPGRQHQANGQTTAHLGA
CCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EIASLAVPEGVTPQLWRSATFSGQAALVTVHEAWNAARLQAVPGHRIGLVVGGTNVQQRD
HHHHHCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCEEEEEECCCCCCHHH
LVLMQDAYRERVPFLRAAYGSTFMDTDLVGLCTQQFAIHGMSFTVGGASASGLLAVIQAA
HHHHHHHHHHHCCHHHHHHCCCHHCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
EAVLSRRVDVCIAVGALMDVSYWECQGLRAMGAMGTDRFAREPERACRPFDRESDGFIFG
HHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCHHHHCCHHHHCCCCCCCCCCEEEC
EACGAVVVESAEHARRRGVTPRGILSGWAMQLDASRGPLSSIEGESQVIGAALRHADLAP
CCCCHHHHHHHHHHHHCCCCCHHHHHCHHEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
ERVDYVNPHGSGSRQGDAIELGALKACGLTHARVNTTKSITGHGLSSAGAVGLIATLVQL
CHHCCCCCCCCCCCCCCEEEECCHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EQGRLHPSLNLVDPIDSSFRWVGATAEAQSLQNALVLAYGFGGINTAVAVRRSATES
HHCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
STSPVQELVVSGFGVTSAIGQGAASFTSALLEGAARFRVMERPGRQHQANGQTTAHLGA
CCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EIASLAVPEGVTPQLWRSATFSGQAALVTVHEAWNAARLQAVPGHRIGLVVGGTNVQQRD
HHHHHCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCEEEEEECCCCCCHHH
LVLMQDAYRERVPFLRAAYGSTFMDTDLVGLCTQQFAIHGMSFTVGGASASGLLAVIQAA
HHHHHHHHHHHCCHHHHHHCCCHHCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
EAVLSRRVDVCIAVGALMDVSYWECQGLRAMGAMGTDRFAREPERACRPFDRESDGFIFG
HHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCHHHHCCHHHHCCCCCCCCCCEEEC
EACGAVVVESAEHARRRGVTPRGILSGWAMQLDASRGPLSSIEGESQVIGAALRHADLAP
CCCCHHHHHHHHHHHHCCCCCHHHHHCHHEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
ERVDYVNPHGSGSRQGDAIELGALKACGLTHARVNTTKSITGHGLSSAGAVGLIATLVQL
CHHCCCCCCCCCCCCCCEEEECCHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EQGRLHPSLNLVDPIDSSFRWVGATAEAQSLQNALVLAYGFGGINTAVAVRRSATES
HHCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]