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The map label for this gene is priA [H]

Identifier: 108763692

GI number: 108763692

Start: 1679934

End: 1682321

Strand: Direct

Name: priA [H]

Synonym: MXAN_1428

Alternate gene names: 108763692

Gene position: 1679934-1682321 (Clockwise)

Preceding gene: 108763419

Following gene: 108762878

Centisome position: 18.38

GC content: 70.98

Gene sequence:

>2388_bases
ATGGAACAGCGACCCTTGTGGAGCGGGGCGGACGCGGCGCCCGCGAAGGCCCCCACGACGCGGGGTTCCAGGGCGGTGCG
CACCTCCGGTGTGAAAAGCCGCCGGGAACTGTCCGAGCCCGCCGCCGCCGCTACAGTGCGCGCCGAAGTGAGCACTCCCG
TCCTGGCCTCCATCGCCGTTGGCCGCCCCGTCCGGGGCGAGTTCACCTACACCCTGCCGGACGAGCTGGCGGGCCGCCTG
GAGCCGGGGCAGCGCGTGCTGGTGCCCTTCGGCCGGGGCACGGCGCTGGGCTTCTACCTGGGGCCCGCGTCTCCGCCGAC
TGGCGAGAAGGTGCGGCTCAAGGCCGTCCAGCGCGTGCTGGAGGACTCTCCGTCCTTGCCCAAGGACCTCATCGCGCTGC
TGCGCTTCGCGGCCGTGCACTACCGCTATCCGCTGGGTGAGGTGATTCGCGGCGCGCTGCCGCCTGGCCTGTCGAAGGCT
GTGGATGAGAAGGAAGCCAGGCCGGACGTGCAGCACTTCGCGGTGGCGCTCGTCAACGAGGTGCCGCCGGAGCTGTCTCG
CGCGCCCGCCCAGTCCGCCGCGCTCGCGTATCTGCTGGCCGTGGGAGGCAGCGCGCCGCTGGAAGAGGTCGCCCACGCGA
TTCCAGGCGCGCGCGAGACGCTGAAGAAGCTGGCCACGCGCGGCTTCGCCCGGCTGGAGGAGAAGACGGTGGAGGCCAGC
GTGAAGGAGGGCCTGATTCAGGGCCGTCCGGACCACCTCACGCCGGAGCAGGACGCGGCGGGCGTGGTGCTGCGCGCGGC
CCTGGACGCGGCCACCTTCCAGCCCTTCCTCCTGCACGGCGTCACCGGCAGCGGGAAGACGGAGGTGTACCTGCGCGCGG
CCGAGCATGCGCTGTCGCTGGGGAAGGGCAGCCTCATCCTGGTGCCGGAAATCGCGCTGACGCCGCAGCTGGTGGGCCGC
TTCCGCAGCCGCTTCGGCGCGGAGGTGGCGGTGCTGCACTCGGCGCTGAAGGACCGGGAGCGGCTCTTCCACTGGCAGGC
GCTGCGCCGCGGCGACGTGAAGATTGCCGTGGGCGTGCGCTCGGCGGTGTTCGCGCCGGTGGACAACCTGGGGCTCATCG
TCGTTGACGAGGAGCACGACCCGTCCTTCAAGCAGGAGGAGAAGCTGCGCTACCAGGCGCGCGACCTGGCCGTCGTGCGC
GGCAAGCAGGCCGGCGCGGTGGTGGTGCTGGGTTCGGCAACGCCCGCGCTGGAGACGCTGGAGAACGTGAAGCGCGGGCG
CTACCAGCTCCTGGAGCTGAAGCACCGCGTGGATGACCGGCCCATGCCCACCATCGAGCTGGTGGACCTGCGCGTGGAGC
GTCCGCGTGAGGGCGTGGTGACGGAAGAGGCGCCCATCCTCAGTCCTCCGTTGCTGGAGGCGATGGCGGAGACGATTGGC
CGCGGTCAGCAGGTCATCCTCTTCCTCAACCGCCGGGGCCACAGCACCGTCCTTCTGTGTGAGGTGTGTGGCCTGTCGCT
CAAGTGCAGCGAGTGTGACGTGTGCCTCACGCACCACCGCTCGCAGAACCGGGTGGTGTGCCACTACTGCGGCCTGGCCA
TGCCGGTGCCGGAGCAGTGTCTGGAGTGCACGGGCCCCATGCTCAAGCTGGGCATCGGCACCGAGCGTGTGGAGGCGGAG
GTGCTGGAGCGCATCCCCACCGCGCGTGTCGCCCGGTTGGACCGGGACTCGGCCAGCAGCGCCGAGCGGCTGACGGAGAT
GCTGGCCTCGTTCGCGCGCCGGGAACTGGATGTCCTGGTGGGCACGCAGATGGTGGCCAAGGGGCACGACTTTCCAGGCG
TGACGCTGGTGTGTGTCGTCATGGCGGACACGTCCCTCTCCATCCCGGACTTCCGTGCCGCCGAGCGCACCTTCCATCTG
CTCACCCAGGTGTCCGGCCGCGCGGGCCGGGGCAAGGACCCGGGCCGGGTGCTGGTGCAGACCTACAATCCGGACGCGGA
GCCGGTGCGGCGCGTGCTGGCCCATGACTTCGACGGCTTCGCCAATCAAGAACTGGAGTGGCGCAAGGCGCTGGCCTACC
CTCCCTATTCACGCATGGCCGCCGTCCGCCTGGAGGGGGAGCACCCCGAACAGACGGCCAGTGTGGCGCGCTCCCTGGGG
AACATCGTCTCCCGGCACATGCCCCCTGCGTCCGCGGGGGTCCGCATGCTGGGGCCGGCCCTGGCGCCCATCTCCCGCAT
CCGTGGCAAGACGCGCTGGCAGCTCCTCTTGAAGGGGCCAACGCATACGGCGCTCGCCCCGCTGCTCGCCCGGCTGGAAG
CGGCCCTGGTCGATGTTCCTTCCGCGGTGAAGGTCGTGATCGACGTGGATCCGGGGGCCATGCTGTAG

Upstream 100 bases:

>100_bases
GTCCCACGGCGGGACCCGGTGGGGAATGTCAGACCCTTCGCGCACTGTGGACCCCGGTAGCAGGACGGATGGGGCCGGGG
TGGTGGTTGAAGGGAGTGTC

Downstream 100 bases:

>100_bases
ACTCGGTGCCCCCCCATGGGCGCACCGGTCCTTCTCGTCCACGACGACATCGCCAATATCGCCGCCGTGCGGCGCCTGCT
CACGCGTGAAGGGTACGAGG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MEQRPLWSGADAAPAKAPTTRGSRAVRTSGVKSRRELSEPAAAATVRAEVSTPVLASIAVGRPVRGEFTYTLPDELAGRL
EPGQRVLVPFGRGTALGFYLGPASPPTGEKVRLKAVQRVLEDSPSLPKDLIALLRFAAVHYRYPLGEVIRGALPPGLSKA
VDEKEARPDVQHFAVALVNEVPPELSRAPAQSAALAYLLAVGGSAPLEEVAHAIPGARETLKKLATRGFARLEEKTVEAS
VKEGLIQGRPDHLTPEQDAAGVVLRAALDAATFQPFLLHGVTGSGKTEVYLRAAEHALSLGKGSLILVPEIALTPQLVGR
FRSRFGAEVAVLHSALKDRERLFHWQALRRGDVKIAVGVRSAVFAPVDNLGLIVVDEEHDPSFKQEEKLRYQARDLAVVR
GKQAGAVVVLGSATPALETLENVKRGRYQLLELKHRVDDRPMPTIELVDLRVERPREGVVTEEAPILSPPLLEAMAETIG
RGQQVILFLNRRGHSTVLLCEVCGLSLKCSECDVCLTHHRSQNRVVCHYCGLAMPVPEQCLECTGPMLKLGIGTERVEAE
VLERIPTARVARLDRDSASSAERLTEMLASFARRELDVLVGTQMVAKGHDFPGVTLVCVVMADTSLSIPDFRAAERTFHL
LTQVSGRAGRGKDPGRVLVQTYNPDAEPVRRVLAHDFDGFANQELEWRKALAYPPYSRMAAVRLEGEHPEQTASVARSLG
NIVSRHMPPASAGVRMLGPALAPISRIRGKTRWQLLLKGPTHTALAPLLARLEAALVDVPSAVKVVIDVDPGAML

Sequences:

>Translated_795_residues
MEQRPLWSGADAAPAKAPTTRGSRAVRTSGVKSRRELSEPAAAATVRAEVSTPVLASIAVGRPVRGEFTYTLPDELAGRL
EPGQRVLVPFGRGTALGFYLGPASPPTGEKVRLKAVQRVLEDSPSLPKDLIALLRFAAVHYRYPLGEVIRGALPPGLSKA
VDEKEARPDVQHFAVALVNEVPPELSRAPAQSAALAYLLAVGGSAPLEEVAHAIPGARETLKKLATRGFARLEEKTVEAS
VKEGLIQGRPDHLTPEQDAAGVVLRAALDAATFQPFLLHGVTGSGKTEVYLRAAEHALSLGKGSLILVPEIALTPQLVGR
FRSRFGAEVAVLHSALKDRERLFHWQALRRGDVKIAVGVRSAVFAPVDNLGLIVVDEEHDPSFKQEEKLRYQARDLAVVR
GKQAGAVVVLGSATPALETLENVKRGRYQLLELKHRVDDRPMPTIELVDLRVERPREGVVTEEAPILSPPLLEAMAETIG
RGQQVILFLNRRGHSTVLLCEVCGLSLKCSECDVCLTHHRSQNRVVCHYCGLAMPVPEQCLECTGPMLKLGIGTERVEAE
VLERIPTARVARLDRDSASSAERLTEMLASFARRELDVLVGTQMVAKGHDFPGVTLVCVVMADTSLSIPDFRAAERTFHL
LTQVSGRAGRGKDPGRVLVQTYNPDAEPVRRVLAHDFDGFANQELEWRKALAYPPYSRMAAVRLEGEHPEQTASVARSLG
NIVSRHMPPASAGVRMLGPALAPISRIRGKTRWQLLLKGPTHTALAPLLARLEAALVDVPSAVKVVIDVDPGAML
>Mature_795_residues
MEQRPLWSGADAAPAKAPTTRGSRAVRTSGVKSRRELSEPAAAATVRAEVSTPVLASIAVGRPVRGEFTYTLPDELAGRL
EPGQRVLVPFGRGTALGFYLGPASPPTGEKVRLKAVQRVLEDSPSLPKDLIALLRFAAVHYRYPLGEVIRGALPPGLSKA
VDEKEARPDVQHFAVALVNEVPPELSRAPAQSAALAYLLAVGGSAPLEEVAHAIPGARETLKKLATRGFARLEEKTVEAS
VKEGLIQGRPDHLTPEQDAAGVVLRAALDAATFQPFLLHGVTGSGKTEVYLRAAEHALSLGKGSLILVPEIALTPQLVGR
FRSRFGAEVAVLHSALKDRERLFHWQALRRGDVKIAVGVRSAVFAPVDNLGLIVVDEEHDPSFKQEEKLRYQARDLAVVR
GKQAGAVVVLGSATPALETLENVKRGRYQLLELKHRVDDRPMPTIELVDLRVERPREGVVTEEAPILSPPLLEAMAETIG
RGQQVILFLNRRGHSTVLLCEVCGLSLKCSECDVCLTHHRSQNRVVCHYCGLAMPVPEQCLECTGPMLKLGIGTERVEAE
VLERIPTARVARLDRDSASSAERLTEMLASFARRELDVLVGTQMVAKGHDFPGVTLVCVVMADTSLSIPDFRAAERTFHL
LTQVSGRAGRGKDPGRVLVQTYNPDAEPVRRVLAHDFDGFANQELEWRKALAYPPYSRMAAVRLEGEHPEQTASVARSLG
NIVSRHMPPASAGVRMLGPALAPISRIRGKTRWQLLLKGPTHTALAPLLARLEAALVDVPSAVKVVIDVDPGAML

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=744, Percent_Identity=38.3064516129032, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 86044; Mature: 86044

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQRPLWSGADAAPAKAPTTRGSRAVRTSGVKSRRELSEPAAAATVRAEVSTPVLASIAV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHCCCHHHHEEEHHCCCCHHHHHHC
GRPVRGEFTYTLPDELAGRLEPGQRVLVPFGRGTALGFYLGPASPPTGEKVRLKAVQRVL
CCCCCCCEEEECCHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
EDSPSLPKDLIALLRFAAVHYRYPLGEVIRGALPPGLSKAVDEKEARPDVQHFAVALVNE
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
VPPELSRAPAQSAALAYLLAVGGSAPLEEVAHAIPGARETLKKLATRGFARLEEKTVEAS
CCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKEGLIQGRPDHLTPEQDAAGVVLRAALDAATFQPFLLHGVTGSGKTEVYLRAAEHALSL
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCEEEEEEHHHHHHC
GKGSLILVPEIALTPQLVGRFRSRFGAEVAVLHSALKDRERLFHWQALRRGDVKIAVGVR
CCCCEEEECCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECH
SAVFAPVDNLGLIVVDEEHDPSFKQEEKLRYQARDLAVVRGKQAGAVVVLGSATPALETL
HHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHHH
ENVKRGRYQLLELKHRVDDRPMPTIELVDLRVERPREGVVTEEAPILSPPLLEAMAETIG
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHC
RGQQVILFLNRRGHSTVLLCEVCGLSLKCSECDVCLTHHRSQNRVVCHYCGLAMPVPEQC
CCCEEEEEEECCCCCEEEEEEHHCCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHH
LECTGPMLKLGIGTERVEAEVLERIPTARVARLDRDSASSAERLTEMLASFARRELDVLV
HHCCCCEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GTQMVAKGHDFPGVTLVCVVMADTSLSIPDFRAAERTFHLLTQVSGRAGRGKDPGRVLVQ
HHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
TYNPDAEPVRRVLAHDFDGFANQELEWRKALAYPPYSRMAAVRLEGEHPEQTASVARSLG
ECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHH
NIVSRHMPPASAGVRMLGPALAPISRIRGKTRWQLLLKGPTHTALAPLLARLEAALVDVP
HHHHHCCCCHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC
SAVKVVIDVDPGAML
CEEEEEEECCCCCCC
>Mature Secondary Structure
MEQRPLWSGADAAPAKAPTTRGSRAVRTSGVKSRRELSEPAAAATVRAEVSTPVLASIAV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHCCCHHHHEEEHHCCCCHHHHHHC
GRPVRGEFTYTLPDELAGRLEPGQRVLVPFGRGTALGFYLGPASPPTGEKVRLKAVQRVL
CCCCCCCEEEECCHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
EDSPSLPKDLIALLRFAAVHYRYPLGEVIRGALPPGLSKAVDEKEARPDVQHFAVALVNE
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
VPPELSRAPAQSAALAYLLAVGGSAPLEEVAHAIPGARETLKKLATRGFARLEEKTVEAS
CCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKEGLIQGRPDHLTPEQDAAGVVLRAALDAATFQPFLLHGVTGSGKTEVYLRAAEHALSL
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCEEEEEEHHHHHHC
GKGSLILVPEIALTPQLVGRFRSRFGAEVAVLHSALKDRERLFHWQALRRGDVKIAVGVR
CCCCEEEECCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECH
SAVFAPVDNLGLIVVDEEHDPSFKQEEKLRYQARDLAVVRGKQAGAVVVLGSATPALETL
HHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHHH
ENVKRGRYQLLELKHRVDDRPMPTIELVDLRVERPREGVVTEEAPILSPPLLEAMAETIG
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHC
RGQQVILFLNRRGHSTVLLCEVCGLSLKCSECDVCLTHHRSQNRVVCHYCGLAMPVPEQC
CCCEEEEEEECCCCCEEEEEEHHCCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHH
LECTGPMLKLGIGTERVEAEVLERIPTARVARLDRDSASSAERLTEMLASFARRELDVLV
HHCCCCEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GTQMVAKGHDFPGVTLVCVVMADTSLSIPDFRAAERTFHLLTQVSGRAGRGKDPGRVLVQ
HHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
TYNPDAEPVRRVLAHDFDGFANQELEWRKALAYPPYSRMAAVRLEGEHPEQTASVARSLG
ECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHH
NIVSRHMPPASAGVRMLGPALAPISRIRGKTRWQLLLKGPTHTALAPLLARLEAALVDVP
HHHHHCCCCHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC
SAVKVVIDVDPGAML
CEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]